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Gene information
General information
TC014911 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid43: 3281757..3285274
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014911
    iBeetle screen iB_02350
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head capsule dorsal decreased -

    head complete not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014911 RA
      MEVLRAVLN TNLVKIPVC LTVHNRSYA RLAEVGRLD 
      APKITGKYE TGQLILHRV FGYRGVVLF PWLARVYDR 
      DLPQHRSGD VEPNPGVGK EVRGRTHTF YQVLIDQRD 
      CPYIRAQTE AVTFLGNQD SSRSLYAIP GLDYVAHED 
      IIPYTSAEK SPLHHELFD KFLAYDPDK DPPFVAQET 
      LRAWQNKNH PWLELSDVH KETTENIRV TVIPFYMGF 
      RESHSTSVY WWRYCIRLE NLGSLSVQL RERHWRIFS 
      LSGTLETVR GRGVVGQEP ALTRTLPAF QYSSHVSLQ 
      APSGHMWGT FRMEREDGY TFDCRIPPF SLESKPDIT 
      PAAPPGAL
    CDS sequence
    • >TC014911 RA
      atggaagtg ttgcgggcc gttttgaac actaatttg 
      gtgaaaatt ccggtgtgt ttgaccgtc cacaaccgc 
      tcgtatgcc aggttggcg gaggtcggg cgtttggac 
      gccccaaaa atcaccggg aagtacgag acgggccag 
      ttgatcctc caccgggtg tttgggtac cgtggggtg 
      gtgctgttc ccgtggctg gcccgtgtg tacgaccgc 
      gatttgccc cagcaccgg tcgggggac gtggagccc 
      aacccgggg gttggcaag gaggtgcgg gggcgcacg 
      cacaccttt tatcaagtt ttgatcgat cagagagac 
      tgtccttat attagagct caaaccgaa gctgtgacc 
      ttcttaggt aatcaggac tcgtccagg agcttgtat 
      gccatccct ggactggat tacgtggcg catgaagat 
      attattcca tacactagt gcggaaaaa tcgccgctg 
      catcatgag ctgtttgat aaattcctg gcttatgat 
      cccgataaa gatccgccg tttgtggct caggagact 
      ctccgagcc tggcaaaac aagaaccac ccttggcta 
      gaactgtcc gatgtccat aaagaaaca actgaaaat 
      attagagtg actgtgatt ccattttat atgggtttt 
      agggaatcc cactccacc tcagtctat tggtggagg 
      tattgtata agattagaa aatttaggt tctctcagt 
      gtccagttg cgtgaacgt cactggcgt attttttcc 
      ttgtctggt actttggaa acagtacgt gggcgtggt 
      gtagtaggc caggaacca gcgcttact aggacatta 
      ccagccttt caatacagt agtcacgtc agtctccaa 
      gcgcctagc ggccacatg tgggggact ttcaggatg 
      gagcgtgag gatgggtac actttcgat tgtcgaatc 
      ccgccattt tcgctcgag agcaagccg gacataaca 
      ccagcagcg ccccctgga gctctatag
    mRNA sequence
    • >TC014911 RA
      ttaacaaat tggaaaaat cggcgcgac cggagcgcc 
      ctctctttc gattacgta aaaatgcgg tctttgcaa 
      aactgacca aaatattat ttttatttt tggttgtgg 
      cgaacaaaa tttgcgttg aatttaccg gtgcgttgt 
      ggttttgta tcagggggc cccaacagc catgtttaa 
      ataaatgac caaagtgcg caaaatatg aaaaatgga 
      agtgttgcg ggccgtttt gaacactaa tttggtgaa 
      aattccggt gtgtttgac cgtccacaa ccgctcgta 
      tgccaggtt ggcggaggt cgggcgttt ggacgcccc 
      aaaaatcac cgggaagta cgagacggg ccagttgat 
      cctccaccg ggtgtttgg gtaccgtgg ggtggtgct 
      gttcccgtg gctggcccg tgtgtacga ccgcgattt 
      gccccagca ccggtcggg ggacgtgga gcccaaccc 
      gggggttgg caaggaggt gcgggggcg cacgcacac 
      cttttatca agttttgat cgatcagag agactgtcc 
      ttatattag agctcaaac cgaagctgt gaccttctt 
      aggtaatca ggactcgtc caggagctt gtatgccat 
      ccctggact ggattacgt ggcgcatga agatattat 
      tccatacac tagtgcgga aaaatcgcc gctgcatca 
      tgagctgtt tgataaatt cctggctta tgatcccga 
      taaagatcc gccgtttgt ggctcagga gactctccg 
      agcctggca aaacaagaa ccacccttg gctagaact 
      gtccgatgt ccataaaga aacaactga aaatattag 
      agtgactgt gattccatt ttatatggg ttttaggga 
      atcccactc cacctcagt ctattggtg gaggtattg 
      tataagatt agaaaattt aggttctct cagtgtcca 
      gttgcgtga acgtcactg gcgtatttt ttccttgtc 
      tggtacttt ggaaacagt acgtgggcg tggtgtagt 
      aggccagga accagcgct tactaggac attaccagc 
      ctttcaata cagtagtca cgtcagtct ccaagcgcc 
      tagcggcca catgtgggg gactttcag gatggagcg 
      tgaggatgg gtacacttt cgattgtcg aatcccgcc 
      attttcgct cgagagcaa gccggacat aacaccagc 
      agcgccccc tggagctct atagtgtca aatataaaa 
      tgattt