| General information | |||
| TC014910 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 8630625..8647692 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014910 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02349 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC014910 RA
MVRVISVHH FAGQHIQTV EQPTACCVA PPDKLLLAL TSNCIEVRD LKNESDVLF TFPSVDEVI QIVHCRNGD FVATLETKF NRQNKDTNF VRVYINWDS VAALQQSKM TSSGVSLGA SECGMVQPM RARIAGRVT PTTNQSELG SLEMIEIPV KRNPNYIEC CQVSGNLVI LSNRIINVY KFQVKTHDI SRMRFIDFE EMGFWLELT FFPQQMALC ENYIACMNR DNMYLFKLV DEGGEGVTT GEHDFNFGH GNDEPIDFA KLINDETFG STKTKLTVH LPSIVRENS IIHKHSPFT FTDREMAAK IHPNSPSDP KLPQIVNLI QLKLIPILV ENAQRQITE EFKCMILKP VYIDENFTK TKDTKTLTS DYRKCLSSV VCMVATQQE GYLHYFCEN EVESDHCVA VYPFTAPVQ RLVMEDYFL HALTETGLE SYTLRFGHK LFRSFEIVD DVNVACPSI SDAICLVGL RPFLGVEQI LLSEHNLVL LANSESSPT HSVSSNSSS NASYWTLYN LELPTPKTI FNDISILAN AHRFTSSQT YCHLMSEAH VILRVALVL KKWGAVEEN VKLIIPVKS NIDDVIETY RTSCALLAD HYIMCASEE DFKLAIPYY KMARVLPSE VLRRVKKVQ EQSNNYVTK GLMHYLKSV LLEIKHSWE ADKFFTSGS KQNFAETIL ELLENHNFE DLPNLILKS SILREYSTD KLIHILTEK LSSEGSKLT EKHLALAVL HTQKCNTSK SHESLAKIP ESELLTSLQ TNWELLFDT TQSFQGKTK IVTTFSEFA VVLITSHPD VLSESLVYL IMEKKAINL NKLIKVFLE YLPSSIGSD SSTASLVLQ KTLELYFQK YFEKFGDSD ISKIIYERG ANEAMKLLV RSYLSQLQI FQLKEDNNR RDEMSSSFS KLEREILGE NEEKSKYYF DDKLDKNSE NYLFSQFRF SYLDKMPPF QIEITSKLY EACLESYTP KDNYRPSDE ADLVLKKLQ AILSSQIVS KQVLGEVGA FLSLNENLR GSLSLQSII SSTNDAIQL LIDACPQCL LQFGKDRFT RTDEWKFLI ASIQRRILR LSQNEVLKR VCFFHKKLL KDILTHVAT SMTLDQLVQ VFPQRISTS DSDNRNSEL GYDLGYLEN ELDVLNEIQ NYEPFVIIC KETMHANQI NKLITTTGQ QLLCTLNL
- >TC014910 RA
atggttcgg gttatctca gtgcaccat ttcgccggc cagcacatt caaaccgtc gaacaaccc acggcatgt tgcgttgcg ccccccgat aagctgctc ctagccctc accagcaac tgcatcgag gtgcgggat ctgaagaac gagtccgat gtcttgttc acgttcccg tcagttgat gaggttatc cagatcgtg cactgtcgc aacggcgat tttgtggcg acgctcgag accaagttc aatcgacag aataaggac acgaacttc gtccgggtt tacattaat tgggacagt gttgcggcg ttgcaacag tcgaagatg acgagcagt ggggtgagc ttgggggcc tccgagtgc gggatggtg cagcccatg cgggcgagg atcgccggg agggtcacc cccacgacg aatcagtcg gagttgggg agtctggag atgattgaa attccagtt aaacgcaat ccgaattat atcgagtgt tgtcaagtc tctggcaat ttggtgatt ttgtcgaat cgaatcatc aatgtttat aaatttcaa gtgaagacg cacgatatt tcaaggatg aggtttatt gattttgaa gagatgggg ttttggctg gagttgacg ttttttccg caacagatg gcgttgtgt gaaaattac atagcctgc atgaatcgg gataatatg tatttgttt aagttggtg gatgaaggg ggtgagggg gtaacgacg ggggaacac gattttaat tttggacat ggcaacgac gaacccatc gactttgca aaactcatc aacgacgaa accttcggt tctacaaaa accaaactc acggtacac ctcccttcc attgttcgc gaaaacagc ataatccac aaacacagc cctttcacg ttcaccgat agagaaatg gcggccaaa atccacccc aactcgccc tccgacccc aaactaccc caaatagtc aacttgatt caactcaaa ctgatcccg attttggtc gaaaacgcc caacgccaa ataaccgaa gagttcaaa tgcatgatt ttgaagcca gtttacatt gacgaaaat ttcaccaaa accaaagac accaaaaca ctgacaagt gactaccgg aagtgtctc agcagtgtt gtgtgcatg gtggccaca caacaggaa ggctatttg cactatttt tgcgaaaat gaggtcgaa agtgaccat tgtgtggcc gtgtacccg tttacggcc cccgtgcag aggctagtc atggaagac tattttttg cacgcctta accgagacc ggccttgag agttacacg ctacgtttc ggacataaa ctcttcagg agtttcgaa atcgtggac gatgttaat gttgcatgt ccatcaatc agcgatgct atttgtcta gtggggctc cgccccttt ttgggggtg gagcaaatt ctcttgtcg gaacacaat ctagtcctc cttgcaaat tcggaaagt tcgccaact cattcggtc agttcgaac agtagttcg aacgcttcc tattggaca ttgtataat ctagaactg ccaacacct aaaactatt ttcaatgac atttcaatc ctggccaat gcgcatcga tttacgtca tcgcagacg tactgtcat ttgatgagc gaagctcac gtgatcttg agggtggcg cttgtgttg aagaaatgg ggcgcggtc gaggaaaat gtcaaattg ataattccc gtcaaaagt aacattgat gatgttatc gaaacgtac agaacgtcg tgtgccctt ttggccgac cattacata atgtgtgcc agtgaagag gattttaag ttggcaata ccgtactac aaaatggcg cgagttctt ccatccgaa gtgttacga agagtgaaa aaagtacaa gaacaatcg aataattac gtaactaaa ggtttaatg cattatttg aaatcggtt ttactggaa attaaacat agttgggaa gcggataaa ttttttacc agcggttcg aaacagaat ttcgctgaa acgattttg gagttgttg gagaatcac aattttgag gatttgcct aatttgata ttgaaaagt agcattttg agggagtat tccacggat aagttgatt cacattttg acagagaaa ttgtccagt gagggctca aaattgaca gaaaaacat ctcgcactt gccgtttta cacacccaa aagtgcaac acatcgaaa tcacacgaa tcgctagcg aaaataccc gaaagcgaa cttttaaca tctttgcaa accaactgg gagttgctt tttgacaca acccagagt tttcaaggc aaaactaaa atcgtgacg actttttcc gaatttgcc gtcgtcttg atcacctca caccctgat gttttgagc gaaagtttg gtttattta ataatggag aagaaagca atcaattta aataaacta attaaggtt tttctcgag tatttaccc tcaagtatc ggcagcgat agcagtact gctagtcta gtcctgcaa aaaacccta gaattgtat ttccagaaa tatttcgaa aaatttggc gattccgat atttcgaaa attatctac gaaagaggg gccaacgaa gcgatgaaa ttattagtt cgttcgtat ttatcgcaa ctccaaatc ttccagttg aaggaagac aataatcga agagatgaa atgtcgtca agtttcagc aaactggaa cgcgagatt ttaggcgag aatgaggaa aaatcaaag tattatttc gacgataag ttggacaaa aactcggag aattacctg ttctcccaa tttcggttc agttatctc gataaaatg ccgccgttt caaatcgag atcacatcg aagttatac gaggcatgt ctcgaaagt tacacacca aaggacaat taccgaccg agtgacgaa gccgacctt gttttgaag aaactgcaa gcgatttta tcgagtcaa atcgtctcg aaacaagtt ctaggcgaa gtgggcgcg tttttgagc ctcaacgaa aatctgaga gggagtttg agcctacaa tcgattatt tcgagcact aatgacgca atacaatta ttaatcgat gcctgtccg cagtgtttg ctccagttc ggcaaagat cggttcaca cgaaccgac gaatggaaa tttttaatt gcatcaata caaagacgc attttaagg ttaagtcag aacgaggtt ctgaaacga gtttgtttc tttcacaag aagctgctg aaagatatt ctaacacat gtggcgaca tctatgacg ttggatcag ttggttcaa gtatttccg caacgtatt agcacttct gattcggat aatcggaac agtgagctg ggttatgat ctcggttat ttggagaat gagttggat gttttgaac gaaattcaa aattatgag ccttttgtg attatttgt aaggagacg atgcacgcc aatcaaatc aacaagctt attacaacc acagggcag caattgttg tgcactctg aatttgtaa
- >TC014910 RA
gcccaaatg tcaaaacaa tcaacaaaa gttgtttaa atttgttaa aaaccatgt aattaagtg tgttttcaa cgaaataaa cccaagtgc gtgtttgtg gtgaccatt ggagtgtcc gtattagtt tgaaatggt tcgggttat ctcagtgca ccatttcgc cggccagca cattcaaac cgtcgaaca acccacggc atgttgcgt tgcgccccc cgataagct gctcctagc cctcaccag caactgcat cgaggtgcg ggatctgaa gaacgagtc cgatgtctt gttcacgtt cccgtcagt tgatgaggt tatccagat cgtgcactg tcgcaacgg cgattttgt ggcgacgct cgagaccaa gttcaatcg acagaataa ggacacgaa cttcgtccg ggtttacat taattggga cagtgttgc ggcgttgca acagtcgaa gatgacgag cagtggggt gagcttggg ggcctccga gtgcgggat ggtgcagcc catgcgggc gaggatcgc cgggagggt cacccccac gacgaatca gtcggagtt ggggagtct ggagatgat tgaaattcc agttaaacg caatccgaa ttatatcga gtgttgtca agtctctgg caatttggt gattttgtc gaatcgaat catcaatgt ttataaatt tcaagtgaa gacgcacga tatttcaag gatgaggtt tattgattt tgaagagat ggggttttg gctggagtt gacgttttt tccgcaaca gatggcgtt gtgtgaaaa ttacatagc ctgcatgaa tcgggataa tatgtattt gtttaagtt ggtggatga agggggtga gggggtaac gacggggga acacgattt taattttgg acatggcaa cgacgaacc catcgactt tgcaaaact catcaacga cgaaacctt cggttctac aaaaaccaa actcacggt acacctccc ttccattgt tcgcgaaaa cagcataat ccacaaaca cagcccttt cacgttcac cgatagaga aatggcggc caaaatcca ccccaactc gccctccga ccccaaact accccaaat agtcaactt gattcaact caaactgat cccgatttt ggtcgaaaa cgcccaacg ccaaataac cgaagagtt caaatgcat gattttgaa gccagttta cattgacga aaatttcac caaaaccaa agacaccaa aacactgac aagtgacta ccggaagtg tctcagcag tgttgtgtg catggtggc cacacaaca ggaaggcta tttgcacta tttttgcga aaatgaggt cgaaagtga ccattgtgt ggccgtgta cccgtttac ggcccccgt gcagaggct agtcatgga agactattt tttgcacgc cttaaccga gaccggcct tgagagtta cacgctacg tttcggaca taaactctt caggagttt cgaaatcgt ggacgatgt taatgttgc atgtccatc aatcagcga tgctatttg tctagtggg gctccgccc ctttttggg ggtggagca aattctctt gtcggaaca caatctagt cctccttgc aaattcgga aagttcgcc aactcattc ggtcagttc gaacagtag ttcgaacgc ttcctattg gacattgta taatctaga actgccaac acctaaaac tattttcaa tgacatttc aatcctggc caatgcgca tcgatttac gtcatcgca gacgtactg tcatttgat gagcgaagc tcacgtgat cttgagggt ggcgcttgt gttgaagaa atggggcgc ggtcgagga aaatgtcaa attgataat tcccgtcaa aagtaacat tgatgatgt tatcgaaac gtacagaac gtcgtgtgc ccttttggc cgaccatta cataatgtg tgccagtga agaggattt taagttggc aataccgta ctacaaaat ggcgcgagt tcttccatc cgaagtgtt acgaagagt gaaaaaagt acaagaaca atcgaataa ttacgtaac taaaggttt aatgcatta tttgaaatc ggttttact ggaaattaa acatagttg ggaagcgga taaattttt taccagcgg ttcgaaaca gaatttcgc tgaaacgat tttggagtt gttggagaa tcacaattt tgaggattt gcctaattt gatattgaa aagtagcat tttgaggga gtattccac ggataagtt gattcacat tttgacaga gaaattgtc cagtgaggg ctcaaaatt gacagaaaa acatctcgc acttgccgt tttacacac ccaaaagtg caacacatc gaaatcaca cgaatcgct agcgaaaat acccgaaag cgaactttt aacatcttt gcaaaccaa ctgggagtt gctttttga cacaaccca gagttttca aggcaaaac taaaatcgt gacgacttt ttccgaatt tgccgtcgt cttgatcac ctcacaccc tgatgtttt gagcgaaag tttggttta tttaataat ggagaagaa agcaatcaa tttaaataa actaattaa ggtttttct cgagtattt accctcaag tatcggcag cgatagcag tactgctag tctagtcct gcaaaaaac cctagaatt gtatttcca gaaatattt cgaaaaatt tggcgattc cgatatttc gaaaattat ctacgaaag aggggccaa cgaagcgat gaaattatt agttcgttc gtatttatc gcaactcca aatcttcca gttgaagga agacaataa tcgaagaga tgaaatgtc gtcaagttt cagcaaact ggaacgcga gattttagg cgagaatga ggaaaaatc aaagtatta tttcgacga taagttgga caaaaactc ggagaatta cctgttctc ccaatttcg gttcagtta tctcgataa aatgccgcc gtttcaaat cgagatcac atcgaagtt atacgaggc atgtctcga aagttacac accaaagga caattaccg accgagtga cgaagccga ccttgtttt gaagaaact gcaagcgat tttatcgag tcaaatcgt ctcgaaaca agttctagg cgaagtggg cgcgttttt gagcctcaa cgaaaatct gagagggag tttgagcct acaatcgat tatttcgag cactaatga cgcaataca attattaat cgatgcctg tccgcagtg tttgctcca gttcggcaa agatcggtt cacacgaac cgacgaatg gaaattttt aattgcatc aatacaaag acgcatttt aaggttaag tcagaacga ggttctgaa acgagtttg tttctttca caagaagct gctgaaaga tattctaac acatgtggc gacatctat gacgttgga tcagttggt tcaagtatt tccgcaacg tattagcac ttctgattc ggataatcg gaacagtga gctgggtta tgatctcgg ttatttgga gaatgagtt ggatgtttt gaacgaaat tcaaaatta tgagccttt tgtgattat ttgtaagga gacgatgca cgccaatca aatcaacaa gcttattac aaccacagg gcagcaatt gttgtgcac tctgaattt gtaattttg tgatttttt tatttatct atcgcttcg accaaaaat tgaaaccaa agcacaaat cggttgcga tgatgatgt tttttacac cctaatgtt atttcgttt gtgtacgag ggtggtcca aaatgtttg gtttctgat agtaatttt tggtgcgcc ctctgtctt gttatctag atttgagat tttatttat tgacgtttt ttattattt ttgggtaag ggaataata agaaacgtc attactgtg atagatttt aatccactc tttagaaaa atcaaactt gatgtgata aaataattc caataaaag tatacattt taa
