Status
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Gene information
General information
TC014910 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8630625..8647692
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014910
    iBeetle screen iB_02349

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014910 RA
      MVRVISVHH FAGQHIQTV EQPTACCVA PPDKLLLAL 
      TSNCIEVRD LKNESDVLF TFPSVDEVI QIVHCRNGD 
      FVATLETKF NRQNKDTNF VRVYINWDS VAALQQSKM 
      TSSGVSLGA SECGMVQPM RARIAGRVT PTTNQSELG 
      SLEMIEIPV KRNPNYIEC CQVSGNLVI LSNRIINVY 
      KFQVKTHDI SRMRFIDFE EMGFWLELT FFPQQMALC 
      ENYIACMNR DNMYLFKLV DEGGEGVTT GEHDFNFGH 
      GNDEPIDFA KLINDETFG STKTKLTVH LPSIVRENS 
      IIHKHSPFT FTDREMAAK IHPNSPSDP KLPQIVNLI 
      QLKLIPILV ENAQRQITE EFKCMILKP VYIDENFTK 
      TKDTKTLTS DYRKCLSSV VCMVATQQE GYLHYFCEN 
      EVESDHCVA VYPFTAPVQ RLVMEDYFL HALTETGLE 
      SYTLRFGHK LFRSFEIVD DVNVACPSI SDAICLVGL 
      RPFLGVEQI LLSEHNLVL LANSESSPT HSVSSNSSS 
      NASYWTLYN LELPTPKTI FNDISILAN AHRFTSSQT 
      YCHLMSEAH VILRVALVL KKWGAVEEN VKLIIPVKS 
      NIDDVIETY RTSCALLAD HYIMCASEE DFKLAIPYY 
      KMARVLPSE VLRRVKKVQ EQSNNYVTK GLMHYLKSV 
      LLEIKHSWE ADKFFTSGS KQNFAETIL ELLENHNFE 
      DLPNLILKS SILREYSTD KLIHILTEK LSSEGSKLT 
      EKHLALAVL HTQKCNTSK SHESLAKIP ESELLTSLQ 
      TNWELLFDT TQSFQGKTK IVTTFSEFA VVLITSHPD 
      VLSESLVYL IMEKKAINL NKLIKVFLE YLPSSIGSD 
      SSTASLVLQ KTLELYFQK YFEKFGDSD ISKIIYERG 
      ANEAMKLLV RSYLSQLQI FQLKEDNNR RDEMSSSFS 
      KLEREILGE NEEKSKYYF DDKLDKNSE NYLFSQFRF 
      SYLDKMPPF QIEITSKLY EACLESYTP KDNYRPSDE 
      ADLVLKKLQ AILSSQIVS KQVLGEVGA FLSLNENLR 
      GSLSLQSII SSTNDAIQL LIDACPQCL LQFGKDRFT 
      RTDEWKFLI ASIQRRILR LSQNEVLKR VCFFHKKLL 
      KDILTHVAT SMTLDQLVQ VFPQRISTS DSDNRNSEL 
      GYDLGYLEN ELDVLNEIQ NYEPFVIIC KETMHANQI 
      NKLITTTGQ QLLCTLNL
    CDS sequence
    • >TC014910 RA
      atggttcgg gttatctca gtgcaccat ttcgccggc 
      cagcacatt caaaccgtc gaacaaccc acggcatgt 
      tgcgttgcg ccccccgat aagctgctc ctagccctc 
      accagcaac tgcatcgag gtgcgggat ctgaagaac 
      gagtccgat gtcttgttc acgttcccg tcagttgat 
      gaggttatc cagatcgtg cactgtcgc aacggcgat 
      tttgtggcg acgctcgag accaagttc aatcgacag 
      aataaggac acgaacttc gtccgggtt tacattaat 
      tgggacagt gttgcggcg ttgcaacag tcgaagatg 
      acgagcagt ggggtgagc ttgggggcc tccgagtgc 
      gggatggtg cagcccatg cgggcgagg atcgccggg 
      agggtcacc cccacgacg aatcagtcg gagttgggg 
      agtctggag atgattgaa attccagtt aaacgcaat 
      ccgaattat atcgagtgt tgtcaagtc tctggcaat 
      ttggtgatt ttgtcgaat cgaatcatc aatgtttat 
      aaatttcaa gtgaagacg cacgatatt tcaaggatg 
      aggtttatt gattttgaa gagatgggg ttttggctg 
      gagttgacg ttttttccg caacagatg gcgttgtgt 
      gaaaattac atagcctgc atgaatcgg gataatatg 
      tatttgttt aagttggtg gatgaaggg ggtgagggg 
      gtaacgacg ggggaacac gattttaat tttggacat 
      ggcaacgac gaacccatc gactttgca aaactcatc 
      aacgacgaa accttcggt tctacaaaa accaaactc 
      acggtacac ctcccttcc attgttcgc gaaaacagc 
      ataatccac aaacacagc cctttcacg ttcaccgat 
      agagaaatg gcggccaaa atccacccc aactcgccc 
      tccgacccc aaactaccc caaatagtc aacttgatt 
      caactcaaa ctgatcccg attttggtc gaaaacgcc 
      caacgccaa ataaccgaa gagttcaaa tgcatgatt 
      ttgaagcca gtttacatt gacgaaaat ttcaccaaa 
      accaaagac accaaaaca ctgacaagt gactaccgg 
      aagtgtctc agcagtgtt gtgtgcatg gtggccaca 
      caacaggaa ggctatttg cactatttt tgcgaaaat 
      gaggtcgaa agtgaccat tgtgtggcc gtgtacccg 
      tttacggcc cccgtgcag aggctagtc atggaagac 
      tattttttg cacgcctta accgagacc ggccttgag 
      agttacacg ctacgtttc ggacataaa ctcttcagg 
      agtttcgaa atcgtggac gatgttaat gttgcatgt 
      ccatcaatc agcgatgct atttgtcta gtggggctc 
      cgccccttt ttgggggtg gagcaaatt ctcttgtcg 
      gaacacaat ctagtcctc cttgcaaat tcggaaagt 
      tcgccaact cattcggtc agttcgaac agtagttcg 
      aacgcttcc tattggaca ttgtataat ctagaactg 
      ccaacacct aaaactatt ttcaatgac atttcaatc 
      ctggccaat gcgcatcga tttacgtca tcgcagacg 
      tactgtcat ttgatgagc gaagctcac gtgatcttg 
      agggtggcg cttgtgttg aagaaatgg ggcgcggtc 
      gaggaaaat gtcaaattg ataattccc gtcaaaagt 
      aacattgat gatgttatc gaaacgtac agaacgtcg 
      tgtgccctt ttggccgac cattacata atgtgtgcc 
      agtgaagag gattttaag ttggcaata ccgtactac 
      aaaatggcg cgagttctt ccatccgaa gtgttacga 
      agagtgaaa aaagtacaa gaacaatcg aataattac 
      gtaactaaa ggtttaatg cattatttg aaatcggtt 
      ttactggaa attaaacat agttgggaa gcggataaa 
      ttttttacc agcggttcg aaacagaat ttcgctgaa 
      acgattttg gagttgttg gagaatcac aattttgag 
      gatttgcct aatttgata ttgaaaagt agcattttg 
      agggagtat tccacggat aagttgatt cacattttg 
      acagagaaa ttgtccagt gagggctca aaattgaca 
      gaaaaacat ctcgcactt gccgtttta cacacccaa 
      aagtgcaac acatcgaaa tcacacgaa tcgctagcg 
      aaaataccc gaaagcgaa cttttaaca tctttgcaa 
      accaactgg gagttgctt tttgacaca acccagagt 
      tttcaaggc aaaactaaa atcgtgacg actttttcc 
      gaatttgcc gtcgtcttg atcacctca caccctgat 
      gttttgagc gaaagtttg gtttattta ataatggag 
      aagaaagca atcaattta aataaacta attaaggtt 
      tttctcgag tatttaccc tcaagtatc ggcagcgat 
      agcagtact gctagtcta gtcctgcaa aaaacccta 
      gaattgtat ttccagaaa tatttcgaa aaatttggc 
      gattccgat atttcgaaa attatctac gaaagaggg 
      gccaacgaa gcgatgaaa ttattagtt cgttcgtat 
      ttatcgcaa ctccaaatc ttccagttg aaggaagac 
      aataatcga agagatgaa atgtcgtca agtttcagc 
      aaactggaa cgcgagatt ttaggcgag aatgaggaa 
      aaatcaaag tattatttc gacgataag ttggacaaa 
      aactcggag aattacctg ttctcccaa tttcggttc 
      agttatctc gataaaatg ccgccgttt caaatcgag 
      atcacatcg aagttatac gaggcatgt ctcgaaagt 
      tacacacca aaggacaat taccgaccg agtgacgaa 
      gccgacctt gttttgaag aaactgcaa gcgatttta 
      tcgagtcaa atcgtctcg aaacaagtt ctaggcgaa 
      gtgggcgcg tttttgagc ctcaacgaa aatctgaga 
      gggagtttg agcctacaa tcgattatt tcgagcact 
      aatgacgca atacaatta ttaatcgat gcctgtccg 
      cagtgtttg ctccagttc ggcaaagat cggttcaca 
      cgaaccgac gaatggaaa tttttaatt gcatcaata 
      caaagacgc attttaagg ttaagtcag aacgaggtt 
      ctgaaacga gtttgtttc tttcacaag aagctgctg 
      aaagatatt ctaacacat gtggcgaca tctatgacg 
      ttggatcag ttggttcaa gtatttccg caacgtatt 
      agcacttct gattcggat aatcggaac agtgagctg 
      ggttatgat ctcggttat ttggagaat gagttggat 
      gttttgaac gaaattcaa aattatgag ccttttgtg 
      attatttgt aaggagacg atgcacgcc aatcaaatc 
      aacaagctt attacaacc acagggcag caattgttg 
      tgcactctg aatttgtaa
    mRNA sequence
    • >TC014910 RA
      gcccaaatg tcaaaacaa tcaacaaaa gttgtttaa 
      atttgttaa aaaccatgt aattaagtg tgttttcaa 
      cgaaataaa cccaagtgc gtgtttgtg gtgaccatt 
      ggagtgtcc gtattagtt tgaaatggt tcgggttat 
      ctcagtgca ccatttcgc cggccagca cattcaaac 
      cgtcgaaca acccacggc atgttgcgt tgcgccccc 
      cgataagct gctcctagc cctcaccag caactgcat 
      cgaggtgcg ggatctgaa gaacgagtc cgatgtctt 
      gttcacgtt cccgtcagt tgatgaggt tatccagat 
      cgtgcactg tcgcaacgg cgattttgt ggcgacgct 
      cgagaccaa gttcaatcg acagaataa ggacacgaa 
      cttcgtccg ggtttacat taattggga cagtgttgc 
      ggcgttgca acagtcgaa gatgacgag cagtggggt 
      gagcttggg ggcctccga gtgcgggat ggtgcagcc 
      catgcgggc gaggatcgc cgggagggt cacccccac 
      gacgaatca gtcggagtt ggggagtct ggagatgat 
      tgaaattcc agttaaacg caatccgaa ttatatcga 
      gtgttgtca agtctctgg caatttggt gattttgtc 
      gaatcgaat catcaatgt ttataaatt tcaagtgaa 
      gacgcacga tatttcaag gatgaggtt tattgattt 
      tgaagagat ggggttttg gctggagtt gacgttttt 
      tccgcaaca gatggcgtt gtgtgaaaa ttacatagc 
      ctgcatgaa tcgggataa tatgtattt gtttaagtt 
      ggtggatga agggggtga gggggtaac gacggggga 
      acacgattt taattttgg acatggcaa cgacgaacc 
      catcgactt tgcaaaact catcaacga cgaaacctt 
      cggttctac aaaaaccaa actcacggt acacctccc 
      ttccattgt tcgcgaaaa cagcataat ccacaaaca 
      cagcccttt cacgttcac cgatagaga aatggcggc 
      caaaatcca ccccaactc gccctccga ccccaaact 
      accccaaat agtcaactt gattcaact caaactgat 
      cccgatttt ggtcgaaaa cgcccaacg ccaaataac 
      cgaagagtt caaatgcat gattttgaa gccagttta 
      cattgacga aaatttcac caaaaccaa agacaccaa 
      aacactgac aagtgacta ccggaagtg tctcagcag 
      tgttgtgtg catggtggc cacacaaca ggaaggcta 
      tttgcacta tttttgcga aaatgaggt cgaaagtga 
      ccattgtgt ggccgtgta cccgtttac ggcccccgt 
      gcagaggct agtcatgga agactattt tttgcacgc 
      cttaaccga gaccggcct tgagagtta cacgctacg 
      tttcggaca taaactctt caggagttt cgaaatcgt 
      ggacgatgt taatgttgc atgtccatc aatcagcga 
      tgctatttg tctagtggg gctccgccc ctttttggg 
      ggtggagca aattctctt gtcggaaca caatctagt 
      cctccttgc aaattcgga aagttcgcc aactcattc 
      ggtcagttc gaacagtag ttcgaacgc ttcctattg 
      gacattgta taatctaga actgccaac acctaaaac 
      tattttcaa tgacatttc aatcctggc caatgcgca 
      tcgatttac gtcatcgca gacgtactg tcatttgat 
      gagcgaagc tcacgtgat cttgagggt ggcgcttgt 
      gttgaagaa atggggcgc ggtcgagga aaatgtcaa 
      attgataat tcccgtcaa aagtaacat tgatgatgt 
      tatcgaaac gtacagaac gtcgtgtgc ccttttggc 
      cgaccatta cataatgtg tgccagtga agaggattt 
      taagttggc aataccgta ctacaaaat ggcgcgagt 
      tcttccatc cgaagtgtt acgaagagt gaaaaaagt 
      acaagaaca atcgaataa ttacgtaac taaaggttt 
      aatgcatta tttgaaatc ggttttact ggaaattaa 
      acatagttg ggaagcgga taaattttt taccagcgg 
      ttcgaaaca gaatttcgc tgaaacgat tttggagtt 
      gttggagaa tcacaattt tgaggattt gcctaattt 
      gatattgaa aagtagcat tttgaggga gtattccac 
      ggataagtt gattcacat tttgacaga gaaattgtc 
      cagtgaggg ctcaaaatt gacagaaaa acatctcgc 
      acttgccgt tttacacac ccaaaagtg caacacatc 
      gaaatcaca cgaatcgct agcgaaaat acccgaaag 
      cgaactttt aacatcttt gcaaaccaa ctgggagtt 
      gctttttga cacaaccca gagttttca aggcaaaac 
      taaaatcgt gacgacttt ttccgaatt tgccgtcgt 
      cttgatcac ctcacaccc tgatgtttt gagcgaaag 
      tttggttta tttaataat ggagaagaa agcaatcaa 
      tttaaataa actaattaa ggtttttct cgagtattt 
      accctcaag tatcggcag cgatagcag tactgctag 
      tctagtcct gcaaaaaac cctagaatt gtatttcca 
      gaaatattt cgaaaaatt tggcgattc cgatatttc 
      gaaaattat ctacgaaag aggggccaa cgaagcgat 
      gaaattatt agttcgttc gtatttatc gcaactcca 
      aatcttcca gttgaagga agacaataa tcgaagaga 
      tgaaatgtc gtcaagttt cagcaaact ggaacgcga 
      gattttagg cgagaatga ggaaaaatc aaagtatta 
      tttcgacga taagttgga caaaaactc ggagaatta 
      cctgttctc ccaatttcg gttcagtta tctcgataa 
      aatgccgcc gtttcaaat cgagatcac atcgaagtt 
      atacgaggc atgtctcga aagttacac accaaagga 
      caattaccg accgagtga cgaagccga ccttgtttt 
      gaagaaact gcaagcgat tttatcgag tcaaatcgt 
      ctcgaaaca agttctagg cgaagtggg cgcgttttt 
      gagcctcaa cgaaaatct gagagggag tttgagcct 
      acaatcgat tatttcgag cactaatga cgcaataca 
      attattaat cgatgcctg tccgcagtg tttgctcca 
      gttcggcaa agatcggtt cacacgaac cgacgaatg 
      gaaattttt aattgcatc aatacaaag acgcatttt 
      aaggttaag tcagaacga ggttctgaa acgagtttg 
      tttctttca caagaagct gctgaaaga tattctaac 
      acatgtggc gacatctat gacgttgga tcagttggt 
      tcaagtatt tccgcaacg tattagcac ttctgattc 
      ggataatcg gaacagtga gctgggtta tgatctcgg 
      ttatttgga gaatgagtt ggatgtttt gaacgaaat 
      tcaaaatta tgagccttt tgtgattat ttgtaagga 
      gacgatgca cgccaatca aatcaacaa gcttattac 
      aaccacagg gcagcaatt gttgtgcac tctgaattt 
      gtaattttg tgatttttt tatttatct atcgcttcg 
      accaaaaat tgaaaccaa agcacaaat cggttgcga 
      tgatgatgt tttttacac cctaatgtt atttcgttt 
      gtgtacgag ggtggtcca aaatgtttg gtttctgat 
      agtaatttt tggtgcgcc ctctgtctt gttatctag 
      atttgagat tttatttat tgacgtttt ttattattt 
      ttgggtaag ggaataata agaaacgtc attactgtg 
      atagatttt aatccactc tttagaaaa atcaaactt 
      gatgtgata aaataattc caataaaag tatacattt 
      taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY