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Gene information
General information
TC014909 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8762723..8772132
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014909
    iBeetle screen iB_02348

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014909 RA
      MTIFRKCIY LFLIFEPIF CANNKFPDD FLFGVASSA 
      YQIEGGYDS RGKTTFDHH WELNSSMVS DSSNAKIAC 
      DSYHQYQKD IELLSYLGV DFYRFSISW ARILPNGFP 
      NKINPDGIR YYNALIDGL LAKNIQPMV TMFHFDLPK 
      PLQDLGGWT NPIIADLFE EYARILFKN FGDRVKYWI 
      TINSNTWGY GDSDWPPMV DQSGFGDYL AIKNTILGH 
      AKVYHLAKG EFREQEAQI GFVVDGRWY EPGSESTQD 
      RDAAERARD FNVGLWLNP IYGSGDFPQ IVKERVKLT 
      SEKEGFAKS RLPQFTADE IKFVKNSFD FLGVNIYTS 
      FLVKDVDEQ NDKEFSWDK DVKAGVYQD VKWEGAKSD 
      WLKVTPWGV RKILSWIKQ KYDNPPIFI TENGFSDAG 
      EIEDLERVN FMKLYLKAL LEAIDRDGV NVKGYAAWS 
      LLDNFEWLV GYTEKFGLY HVDFADPGR RRTPKTSSK 
      WYKKLIERR QLDETIPSD TYKTEL
    CDS sequence
    • >TC014909 RA
      atgactatt tttcgtaaa tgtatttat ttattttta 
      atttttgaa ccaatattc tgtgcaaat aataaattt 
      cccgacgat tttctgttc ggagtcgcg agttcggct 
      tatcaaatt gaaggtggc tatgatagt agaggaaaa 
      accactttt gaccaccac tgggagctg aattcctcc 
      atggtatcc gactcctcc aatgccaaa atcgcctgt 
      gactcctac caccaatac caaaaagac atcgagctt 
      ttatcctac ttgggagtc gatttttac cggttttcc 
      atctcctgg gcccgaatt ttaccaaat gggttccct 
      aacaaaatc aatccagac ggcatccgt tactacaac 
      gccctaatc gatggtctt ttggccaaa aatatccaa 
      cccatggtc acaatgttc cactttgat ttacccaaa 
      ccccttcag gacctcggt gggtggaca aaccccata 
      attgctgac cttttcgag gaatacgct cgcattttg 
      ttcaaaaat tttggcgat agagtcaag tactggatt 
      accattaac tcgaacact tgggggtat ggtgacagt 
      gactggccc ccaatggta gaccagtcg ggatttggg 
      gattattta gccatcaag aacactatt ttgggacac 
      gctaaagtg taccatttg gccaagggc gagtttagg 
      gagcaagag gcccagatt gggttcgtg gtcgatggg 
      cgatggtac gaaccaggg agcgagtcc acccaagac 
      cgtgacgcc gcagaacgt gcaagggat tttaacgtc 
      gggttgtgg cttaatccc atttatggt agtggcgat 
      tttccccaa attgtcaaa gaaagagtg aaattaaca 
      agcgagaaa gagggcttt gccaaatca aggctaccc 
      caatttacc gcagacgaa ataaaattt gtgaaaaat 
      agtttcgat tttctgggg gttaatatc tatacgtct 
      tttctggtc aaagatgtg gatgaacaa aatgataaa 
      gagttttcg tgggataaa gacgtaaag gcgggggtt 
      tatcaagat gtgaagtgg gaaggggcg aagtctgat 
      tggttgaaa gtgacgccc tggggggtg cgaaaaatt 
      ttgagctgg attaaacaa aaatacgat aacccgcca 
      atttttatt acagagaat gggttttcg gatgcgggc 
      gaaattgag gatttagag agggttaat tttatgaag 
      ttgtatctt aaggcttta cttgaagcc attgacagg 
      gatggagtt aatgtgaaa ggctatgct gcttggagt 
      ttattagac aacttcgaa tggcttgta ggatatacg 
      gaaaagttc ggtttgtat catgtggac ttcgccgac 
      cctggtcgc aggcgaact ccgaaaact tcatcaaag 
      tggtacaag aaactgatc gagcgaaga caattggac 
      gaaacaatt ccctccgat acgtacaaa acagaacta 
      taa
    mRNA sequence
    • >TC014909 RA
      acacaacaa acatgagtt gttaatttg ttgtagtat 
      gatgtaacg tgaaggtcg tcagtttct tgttatcga 
      tcaaaatga ctatttttc gtaaatgta tttatttat 
      ttttaattt ttgaaccaa tattctgtg caaataata 
      aatttcccg acgattttc tgttcggag tcgcgagtt 
      cggcttatc aaattgaag gtggctatg atagtagag 
      gaaaaacca cttttgacc accactggg agctgaatt 
      cctccatgg tatccgact cctccaatg ccaaaatcg 
      cctgtgact cctaccacc aataccaaa aagacatcg 
      agcttttat cctacttgg gagtcgatt tttaccggt 
      tttccatct cctgggccc gaattttac caaatgggt 
      tccctaaca aaatcaatc cagacggca tccgttact 
      acaacgccc taatcgatg gtcttttgg ccaaaaata 
      tccaaccca tggtcacaa tgttccact ttgatttac 
      ccaaacccc ttcaggacc tcggtgggt ggacaaacc 
      ccataattg ctgaccttt tcgaggaat acgctcgca 
      ttttgttca aaaattttg gcgatagag tcaagtact 
      ggattacca ttaactcga acacttggg ggtatggtg 
      acagtgact ggcccccaa tggtagacc agtcgggat 
      ttggggatt atttagcca tcaagaaca ctattttgg 
      gacacgcta aagtgtacc atttggcca agggcgagt 
      ttagggagc aagaggccc agattgggt tcgtggtcg 
      atgggcgat ggtacgaac cagggagcg agtccaccc 
      aagaccgtg acgccgcag aacgtgcaa gggatttta 
      acgtcgggt tgtggctta atcccattt atggtagtg 
      gcgattttc cccaaattg tcaaagaaa gagtgaaat 
      taacaagcg agaaagagg gctttgcca aatcaaggc 
      taccccaat ttaccgcag acgaaataa aatttgtga 
      aaaatagtt tcgattttc tgggggtta atatctata 
      cgtcttttc tggtcaaag atgtggatg aacaaaatg 
      ataaagagt tttcgtggg ataaagacg taaaggcgg 
      gggtttatc aagatgtga agtgggaag gggcgaagt 
      ctgattggt tgaaagtga cgccctggg gggtgcgaa 
      aaattttga gctggatta aacaaaaat acgataacc 
      cgccaattt ttattacag agaatgggt tttcggatg 
      cgggcgaaa ttgaggatt tagagaggg ttaatttta 
      tgaagttgt atcttaagg ctttacttg aagccattg 
      acagggatg gagttaatg tgaaaggct atgctgctt 
      ggagtttat tagacaact tcgaatggc ttgtaggat 
      atacggaaa agttcggtt tgtatcatg tggacttcg 
      ccgaccctg gtcgcaggc gaactccga aaacttcat 
      caaagtggt acaagaaac tgatcgagc gaagacaat 
      tggacgaaa caattccct ccgatacgt acaaaacag 
      aactataat aaattcttg ttgtttgtt tttttttgt 
      tgttgttta ataaaataa acttgtcgt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY