Status
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Gene information
General information
TC014904 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8831254..8837011
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014904
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02347

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014904 RA
      MSSNNRPEA GGEAPNTTA SESTLLSPS STYFADEKI 
      QIPETDTSR FSFRKLWAF TGPGFLMSI AYLDPGNIE 
      SDLQSGTVA QYKLLWVLL SATMLGLLM QRLSARLGV 
      VTGLHLAEM CFRQYRRIP RLILWIMIE IAIIGSDMQ 
      EVIGTAIAI YLLSNKTIP LWGGVLITI VDTFTFLFL 
      DKYGLRKLE MFFGLLITV MGLTFGFEY ITSKPDQLQ 
      VLEGMFVPW CEGCDSRAL LQAVGIVGA VIMPHNLYL 
      HSALVKSRD IDRTKPEKV RDANLYFFV EAAIALFVS 
      FVINVFVVA VFAHGLFHK TNKEVHTLC TKYPSVNAS 
      IFPINDDYV EADLYKGGI FLGCTYGLL AMYIWAVGI 
      LAAGQSSTM TGTYAGQFT MEGFLNLQW ARWKRVLFT 
      RTIAIVPTF CLAFFSHIE DLTGMNDVL NAIMSLQLP 
      FATIPTIAF TSNPQIMGE FVNGWFNKI ASILLSILV 
      IGINTYFVV ETMNELHLH WYALVFVVI IGIFYLIFC 
      AYLVIHMAI SMGNTNLLR YHLVKKYIM APIESQLSV 
      NPVSYSRSQ SDWTLDGNA TEIARF
    CDS sequence
    • >TC014904 RA
      atgtcgtca aataaccgc ccagaggcc gggggcgaa 
      gcccccaac accacggcc agcgaatct acgctcctg 
      agcccctca tcaacgtac ttcgccgat gaaaaaatc 
      caaatcccg gagacggac acgtcacgt ttcagtttc 
      cgaaaactg tgggctttc acaggccct ggcttcctc 
      atgtctatc gcttacctt gaccctgga aacatcgag 
      agtgacctc cagagtggc acagtggcc caatataaa 
      cttctatgg gtgttgtta agtgccacg atgttgggg 
      ctcctgatg caaagactc tccgcccgc ttgggggtt 
      gtcacaggg ctacatctc gccgagatg tgtttcagg 
      caatatcgg cgaatcccc cgtttaatt ttatggatt 
      atgatcgag attgcaatc attggatcc gacatgcaa 
      gaagtaatc gggacagca atcgctatt tatttatta 
      tcgaacaaa acgattccg ctctggggc ggtgtttta 
      atcacgatt gtggacact ttcacgttc cttttcctg 
      gacaagtac gggctgagg aaactggag atgtttttc 
      gggctcttg attacagta atggggctc acgttcggt 
      ttcgagtac ataacctca aaaccggac caattgcaa 
      gtgctagaa gggatgttc gtgccctgg tgtgagggt 
      tgcgactcc agggcgctg ctccaagct gtggggatc 
      gtaggtgct gtaattatg cctcacaat ttgtatttg 
      cactcagcg ttggttaag tcgcgcgat atcgatcga 
      acaaaaccg gaaaaggtc cgtgacgca aatttgtac 
      tttttcgtc gaggcggca attgccctt tttgtcagt 
      ttcgtaata aacgtgttc gtggttgcc gtttttgcg 
      catggactt ttccacaaa acgaacaaa gaagtgcac 
      actttgtgt acgaaatat ccgtctgtg aacgcgtca 
      atttttccc attaatgac gattacgtc gaggctgat 
      ttgtacaaa ggggggatt tttttgggg tgcacttat 
      gggctcctt gcgatgtac atttgggcg gttggtatt 
      ctggccgct ggtcaaagt tcgaccatg acggggacc 
      tatgctggg caatttacc atggaaggg tttttaaac 
      ctgcaatgg gcgcgatgg aagagagtt ttgttcact 
      cgtaccatc gcaatcgtg ccgacgttt tgtcttgcc 
      ttcttcagt cacatcgag gatttgaca ggaatgaac 
      gacgtcctc aacgccatc atgagcctc caattaccc 
      ttcgccacc atcccaacc atcgccttc accagcaat 
      ccacaaatc atgggcgaa ttcgtcaat ggatggttc 
      aacaaaatc gcatcgatc ctactcagc attttggtg 
      atcggaatc aacacatat tttgtcgtt gaaacaatg 
      aacgagcta cacctccac tggtacgct ctcgttttc 
      gtcgtcata atcggaatt ttctacttg atcttttgc 
      gcatatctc gtaatccac atggccata agtatggga 
      aatacaaat cttttgcgc taccatcta gtcaagaaa 
      tacattatg gcccccatc gagtcacaa ttatccgtc 
      aatcctgtc agctattcg aggtcgcaa tcagactgg 
      accttggac ggtaatgcg acggaaatt gcgaggttt 
      tga
    mRNA sequence
    • >TC014904 RA
      tccggctct gtctgccgt cgtgtcgat tcgtccgaa 
      ttcgaacgg ttgcacttt gaggttatc gcaatgatg 
      tgaacttga ttttgccac aaatgtcgt caaataacc 
      gcccagagg ccgggggcg aagccccca acaccacgg 
      ccagcgaat ctacgctcc tgagcccct catcaacgt 
      acttcgccg atgaaaaaa tccaaatcc cggagacgg 
      acacgtcac gtttcagtt tccgaaaac tgtgggctt 
      tcacaggcc ctggcttcc tcatgtcta tcgcttacc 
      ttgaccctg gaaacatcg agagtgacc tccagagtg 
      gcacagtgg cccaatata aacttctat gggtgttgt 
      taagtgcca cgatgttgg ggctcctga tgcaaagac 
      tctccgccc gcttggggg ttgtcacag ggctacatc 
      tcgccgaga tgtgtttca ggcaatatc ggcgaatcc 
      cccgtttaa ttttatgga ttatgatcg agattgcaa 
      tcattggat ccgacatgc aagaagtaa tcgggacag 
      caatcgcta tttatttat tatcgaaca aaacgattc 
      cgctctggg gcggtgttt taatcacga ttgtggaca 
      ctttcacgt tccttttcc tggacaagt acgggctga 
      ggaaactgg agatgtttt tcgggctct tgattacag 
      taatggggc tcacgttcg gtttcgagt acataacct 
      caaaaccgg accaattgc aagtgctag aagggatgt 
      tcgtgccct ggtgtgagg gttgcgact ccagggcgc 
      tgctccaag ctgtgggga tcgtaggtg ctgtaatta 
      tgcctcaca atttgtatt tgcactcag cgttggtta 
      agtcgcgcg atatcgatc gaacaaaac cggaaaagg 
      tccgtgacg caaatttgt actttttcg tcgaggcgg 
      caattgccc tttttgtca gtttcgtaa taaacgtgt 
      tcgtggttg ccgtttttg cgcatggac ttttccaca 
      aaacgaaca aagaagtgc acactttgt gtacgaaat 
      atccgtctg tgaacgcgt caatttttc ccattaatg 
      acgattacg tcgaggctg atttgtaca aagggggga 
      tttttttgg ggtgcactt atgggctcc ttgcgatgt 
      acatttggg cggttggta ttctggccg ctggtcaaa 
      gttcgacca tgacgggga cctatgctg ggcaattta 
      ccatggaag ggtttttaa acctgcaat gggcgcgat 
      ggaagagag ttttgttca ctcgtacca tcgcaatcg 
      tgccgacgt tttgtcttg ccttcttca gtcacatcg 
      aggatttga caggaatga acgacgtcc tcaacgcca 
      tcatgagcc tccaattac ccttcgcca ccatcccaa 
      ccatcgcct tcaccagca atccacaaa tcatgggcg 
      aattcgtca atggatggt tcaacaaaa tcgcatcga 
      tcctactca gcattttgg tgatcggaa tcaacacat 
      attttgtcg ttgaaacaa tgaacgagc tacacctcc 
      actggtacg ctctcgttt tcgtcgtca taatcggaa 
      ttttctact tgatctttt gcgcatatc tcgtaatcc 
      acatggcca taagtatgg gaaatacaa atcttttgc 
      gctaccatc tagtcaaga aatacatta tggccccca 
      tcgagtcac aattatccg tcaatcctg tcagctatt 
      cgaggtcgc aatcagact ggaccttgg acggtaatg 
      cgacggaaa ttgcgaggt tttgacgtc aattttagt 
      aaatttgta gtttttttc atgatattt gtgtgatat 
      ttgcgttat gtaatgaaa tatatttta ttagaattt 
      ttcaagttc ggaatttta tttcacctg attcaccaa 
      ggattgggg tttgggtgg tattaatac gccgatcgg 
      ctgttttag ttttgtatt ttcgagtgc cacaatttt 
      gttcgaaaa cgacaatta acaataagt atatttggc 
      cttgacagt agatactgt tatcacgaa acgcaaatt 
      aagctagaa atcagtcgt tgcttttaa atacaaaat 
      aaaaggttg gtggcactt atggagaac tataactta 
      ggaaaggaa acgagatac aaatttaaa ttcagtaat 
      tacttggaa aaaatgtcc tttatactc ttaacagac 
      agaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY