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Gene information
General information
TC014893 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9025263..9030130
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014893
    iBeetle screen iB_02344

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4

    tropharium irregular - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    Pupal morphology
    wings not closed - Number of animals showing the phenotype: 6

    wings potentiallyshortened - Number of animals showing the phenotype: 6
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014893 RA
      MAGKKIRSR TSQNSISVS SLSNRTLNV ATSSNPVRG 
      KPADELLAM TKEQLKNEC RKKGQKTTG NKIELLQRL 
      GYKMTKRRE NSTNGAAVT PDETPTGGG DLTERTRKE 
      KERKERESL VLWKRPLTT LEYSTKEVI VLLTTYGRK 
      ILEHKKIVL FVTLTLLSS YLLVKTAGP HQSYIQIVY 
      KQLLWCSYW IGLGILSSV GLGTGLHTF LLYLGPHIA 
      AVTLAAYEC ESLNFPEPP YPDEIICPD ERNPLAVSI 
      LSIMSKVRL EAMCWGAGT ALGELPPYF MARAARLSG 
      IDPDDEDDD LKEFEELKR KQNNKTELT LIEKGKLFV 
      EELVQRVGF LGILACASI PNPLFDLAG ITCGHFLVP 
      FWTFFGATL IGKAIIKMH IQKLFVIIA FNETLIVTA 
      LQWLKYIPV VGEKLQVPF KAFLDGQKS KLHQSGGGA 
      KVESGNVLS SIFEKFVVA MILYFIVSI VNSFAQSYY 
      KRIHKKKTT KVAKD
    CDS sequence
    • >TC014893 RA
      atggcaggg aaaaaaatc cgctcgaga acgtcacaa 
      aattcgatt tctgtcagt tctctcagt aatcgaaca 
      ctgaatgtg gccacgagc agcaacccc gtgaggggc 
      aaacccgcc gatgaactc cttgctatg accaaggaa 
      cagctcaag aacgagtgc aggaaaaag ggacaaaag 
      actaccggc aataagata gagctgctc caaagactc 
      ggctacaaa atgacgaaa cgtcgcgaa aactccacc 
      aatggggcg gcggtgacc ccagacgag accccaacc 
      gggggcggc gacttaacc gaacgaaca aggaaagag 
      aaagaacga aaagagagg gaaagtcta gtgttatgg 
      aaacgacca ctaactacg ctcgaatat tcgacaaaa 
      gaagtcata gttttacta acaacgtat ggtagaaaa 
      atactagaa cataagaaa atcgtccta tttgtcaca 
      ctaacgcta ctctcatcg tatctattg gtgaaaacg 
      gccggtcct caccaatca tacatccaa atcgtttat 
      aagcaactc ctctggtgt tcgtattgg ataggttta 
      ggtattttg tcatcggtg ggcttgggg acaggttta 
      cacactttc ctactttac ttgggcccc catatagcg 
      gccgtgacg ctcgctgcg tacgaatgc gagtcgtta 
      aattttcca gagccgcct tatcccgat gaaataata 
      tgtccagat gaacgaaat ccattggct gtgtctatt 
      ttaagtatc atgtcgaaa gtacgattg gaggcgatg 
      tgctggggg gcggggact gccttggga gagttgccc 
      ccttatttc atggcccgc gcggcgcga ctctccggt 
      atcgatccc gatgacgaa gacgacgat ttgaaagag 
      tttgaagag ttgaaacgg aaacaaaac aacaaaacc 
      gagctcact ttgatcgaa aagggaaaa ctgtttgtc 
      gaggagctg gtccagagg gttgggttt ttagggatt 
      ttggcgtgt gcaagtata ccgaatcca ttgtttgac 
      ttggccggg atcacgtgt ggtcatttt ttggtcccg 
      ttttggaca ttttttggg gctactctt atcggtaaa 
      gccatcatc aagatgcac atccagaaa ttgtttgtt 
      ataattgca ttcaatgaa acacttatt gttacggcc 
      ctgcaatgg ctcaagtac atacctgtc gtgggcgag 
      aagctacaa gtacctttc aaagccttc ttagatggc 
      caaaaatcg aagttgcat caaagtggc gggggagca 
      aaggtcgaa agtgggaat gttttaagt tcgattttt 
      gagaaattc gttgttgcc atgattttg tattttatc 
      gtgtcgata gtcaattcg tttgctcag agttactat 
      aagcggata cacaagaag aaaactact aaggtagca 
      aaagactag
    mRNA sequence
    • >TC014893 RA
      acttacctg ttaaaaaca aatttgaac tgtcaactg 
      tctaccaca gaatacaca ctcactcca gcgcccccc 
      tgtcatacg tcactcatc aacttttac tcttgagtt 
      tgtgtgatg tgtgctttt ctttccaat tttcttcac 
      aactaacac aaccgggag ctgcatggc agggaaaaa 
      aatccgctc gagaacgtc acaaaattc gatttctgt 
      cagttctct cagtaatcg aacactgaa tgtggccac 
      gagcagcaa ccccgtgag gggcaaacc cgccgatga 
      actccttgc tatgaccaa ggaacagct caagaacga 
      gtgcaggaa aaagggaca aaagactac cggcaataa 
      gatagagct gctccaaag actcggcta caaaatgac 
      gaaacgtcg cgaaaactc caccaatgg ggcggcggt 
      gaccccaga cgagacccc aaccggggg cggcgactt 
      aaccgaacg aacaaggaa agagaaaga acgaaaaga 
      gagggaaag tctagtgtt atggaaacg accactaac 
      tacgctcga atattcgac aaaagaagt catagtttt 
      actaacaac gtatggtag aaaaatact agaacataa 
      gaaaatcgt cctatttgt cacactaac gctactctc 
      atcgtatct attggtgaa aacggccgg tcctcacca 
      atcatacat ccaaatcgt ttataagca actcctctg 
      gtgttcgta ttggatagg tttaggtat tttgtcatc 
      ggtgggctt ggggacagg tttacacac tttcctact 
      ttacttggg cccccatat agcggccgt gacgctcgc 
      tgcgtacga atgcgagtc gttaaattt tccagagcc 
      gccttatcc cgatgaaat aatatgtcc agatgaacg 
      aaatccatt ggctgtgtc tattttaag tatcatgtc 
      gaaagtacg attggaggc gatgtgctg gggggcggg 
      gactgcctt gggagagtt gccccctta tttcatggc 
      ccgcgcggc gcgactctc cggtatcga tcccgatga 
      cgaagacga cgatttgaa agagtttga agagttgaa 
      acggaaaca aaacaacaa aaccgagct cactttgat 
      cgaaaaggg aaaactgtt tgtcgagga gctggtcca 
      gagggttgg gtttttagg gattttggc gtgtgcaag 
      tataccgaa tccattgtt tgacttggc cgggatcac 
      gtgtggtca ttttttggt cccgttttg gacattttt 
      tggggctac tcttatcgg taaagccat catcaagat 
      gcacatcca gaaattgtt tgttataat tgcattcaa 
      tgaaacact tattgttac ggccctgca atggctcaa 
      gtacatacc tgtcgtggg cgagaagct acaagtacc 
      tttcaaagc cttcttaga tggccaaaa atcgaagtt 
      gcatcaaag tggcggggg agcaaaggt cgaaagtgg 
      gaatgtttt aagttcgat ttttgagaa attcgttgt 
      tgccatgat tttgtattt tatcgtgtc gatagtcaa 
      ttcgtttgc tcagagtta ctataagcg gatacacaa 
      gaagaaaac tactaaggt agcaaaaga ctaggtcag 
      ggttatcac tttagtttg ttgttgttt ttattggag 
      gttattttt ttgttgaac ccgcagctt ccttggtta 
      atggaggga gtttcccga cttgcttca ttagtcaag 
      gaaggctgt ggttgaacc attccgatt tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY