General information | |||
TC014882 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 9169954..9191106 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC014882 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02343 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
- >TC014882 RA
MNELDSLRQ EAETLKNAI RDARKAACD TSLVQATAS LEPIGRVQM RTRRTLRGH LAKIYAMHW GSDSRNLVS ASQDGKLIV WDSHTTNKV HAIPLRSSW VMTCAYAPS GSYVACGGL DNICSIYSL KTREGNVRV SRELPGHTG YLSCCRFLD DNQIVTSSG DMSCALWDI ETGQQVTSF LGHTGDVMS LSLSPDMRT FVSGACDAS AKLWDIREG LCKQTFPGH ESDINAVTF FPNGFAFAT GSDDATCRL FDIRADQEL AMYSHDNII CGITSVAFS KSGRLLLAG YDDFNCNVW DSMKTERAG ILAGHDNRV SCLGVTENG MAVGTGSWD SFLRIWN
- >TC014882 RA
atgaacgaa ttggattcc ttgagacaa gaagctgag acactcaaa aatgccatc agagatgcc cgtaaagcg gcttgcgac acatccttg gtccaggca actgccagc cttgaaccc attggaagg gtgcaaatg cgaactaga cgcaccctt cgggggcac ttggcaaaa atctacgcc atgcattgg ggttccgac agtcgtaac ttggtatcg gcctctcag gacggtaaa ctcatcgtc tgggattcc cacaccacc aacaaggtt cacgcgatc ccgttgcga tcctcatgg gtgatgaca tgcgcgtac gctccatca gggagttac gtagcatgc ggaggcctg gacaacatt tgctcgatc tacagccta aaaactcgc gaaggcaac gtacgagtg agcagagaa ctgcccggt cacaccggg tatttgtcg tgctgtcgc ttccttgat gataaccag atagtgacc agttcgggc gacatgagt tgcgccctc tgggacata gagaccggc cagcaggtc acctcattt cttggccac accggcgac gtgatgtcg ctgtcgttg tcgccagac atgcgcacg ttcgtttcg ggagcgtgc gatgcatcg gcgaagtta tgggatata cgagaagga ttatgcaag caaacgttc ccaggacac gagtcggac attaatgcc gtcacgttc tttcctaat ggttttgcg tttgcgacg ggtagtgat gacgccact tgtaggctc ttcgatatc agggccgat caggaacta gccatgtat agtcatgat aacattata tgcggaatc accagtgtc gcattcagt aaatctgga aggttactg cttgcagga tacgacgat ttcaattgt aatgtctgg gattcgatg aaaacagaa cgagcaggt atcctagcg ggccacgac aatcgagta agctgtcta ggcgtaaca gagaacgga atggcggta ggaaccgga tcttgggac agtttccta cgcatttgg aactag
- >TC014882 RA
cagaaatgg ccgttctga cgtcacgaa tcggggact ggaatcgtt accgcaagg gcaccggcg attctcgca ccgcaccca agtaggaaa gcgagagga aaattcgcg tattaaaaa cattgtagg tgtgaaata ggtgcgcga aagtgtcgt taatcgtat gtacggtaa ataaaataa tctcacatg ttcataatt agtggacga gtgaatttg tatgggtgt aagcggatg tattcgtga ttttgtgag aagcaccat aggatagta tagtcgata gtggattgg gacagacaa agccgattg gaaaagcgt tcgttgtgc catagagag tgttgggca gtatgaacg aattggatt ccttgagac aagaagctg agacactca aaaatgcca tcagagatg cccgtaaag cggcttgcg acacatcct tggtccagg caactgcca gccttgaac ccattggaa gggtgcaaa tgcgaacta gacgcaccc ttcgggggc acttggcaa aaatctacg ccatgcatt ggggttccg acagtcgta acttggtat cggcctctc aggacggta aactcatcg tctgggatt cccacacca ccaacaagg ttcacgcga tcccgttgc gatcctcat gggtgatga catgcgcgt acgctccat cagggagtt acgtagcat gcggaggcc tggacaaca tttgctcga tctacagcc taaaaactc gcgaaggca acgtacgag tgagcagag aactgcccg gtcacaccg ggtatttgt cgtgctgtc gcttccttg atgataacc agatagtga ccagttcgg gcgacatga gttgcgccc tctgggaca tagagaccg gccagcagg tcacctcat ttcttggcc acaccggcg acgtgatgt cgctgtcgt tgtcgccag acatgcgca cgttcgttt cgggagcgt gcgatgcat cggcgaagt tatgggata tacgagaag gattatgca agcaaacgt tcccaggac acgagtcgg acattaatg ccgtcacgt tctttccta atggttttg cgtttgcga cgggtagtg atgacgcca cttgtaggc tcttcgata tcagggccg atcaggaac tagccatgt atagtcatg ataacatta tatgcggaa tcaccagtg tcgcattca gtaaatctg gaaggttac tgcttgcag gatacgacg atttcaatt gtaatgtct gggattcga tgaaaacag aacgagcag gtatcctag cgggccacg acaatcgag taagctgtc taggcgtaa cagagaacg gaatggcgg taggaaccg gatcttggg acagtttcc tacgcattt ggaactaga cagcgttgc caaaaatta aatgtaatt attcgtatc tcacctgca tcacagcat tgatttgtt tttcaaaaa taacttcca tgtttcaac aagcattta aaccgtgtc gaagttcca agcatttta attattttt tgtattata ggattttat ctgttcttg ttctctccg cttatgtag tcaccttga tttttataa gagtatgta gcttgtgat ttgtttgtt tgtaaggta tctgtattt attattaca cgtatttta aagaagaaa aaaattata aataaagca gtattttta gttttttct tttttgttt ttctctttc gtttttttt tttttcaaa tgtgatgtg ggtgagatt gaaccgagt cgaggagtg tattagttc caaaacctt tggttagaa tcaagtgca aaacgcaag accaatgtt aggaaataa aagtttttg catttttac atgagcgaa tttgggttg agtgcgagt ggtggttcc caaaacaaa cagaatttt ttacacgaa attattgcc tatttgtcc atataaaaa tgcaataat tttgatttt acttctcat ggcatatgc tactagtcg gtttaccaa aaaaagcag aaaccatgt gccataacc tgaaacttt ccgatgatc atagccgtc gacatcaat cagcgtcac aaagtacca aattgattt tccgctgtg acgtcataa ccaaatgcg tttgtgtaa gaactggca ggttgtaaa aatcaaaca gttttgaga tactgcctc aatatttta acctgttaa aaacagtgt tccattaat taatttcac acttcataa ataaagagg gaaaaacac aaaaaaatc tcatcacgt gatcaaatc actcaattg tttcggtta cgaatcacc gtttcgttt tgtacatat tgagttcct tattgtatt tgcattttt aggatacag tcatgtctt taaataaaa taggtgaaa aaaaaagaa aaaatttac gataatata atactaaaa ctagcttct gcttgtcat tgattgaat gtgaaataa acagtgatg tatgtttac caactcttg acacaaaga aactgtttt ataagccaa aaatttcac ttatttgac tttgatttc atcgcactg cagtcggcg tggcgcatt cagacttcg cggcggatg cgccacgcc gcctttttt taaattaaa ttatttata acgtgtgag tgcgtgagt ggccggcgc gagtcgagg gcgcctctc gcgcctgtg ctcgaactt tgcgcaatc gcaaccaat ttacaatta acaaacaag ggatccaac agctcgtcc gttgttcga aagggctca tggattggt tgtcgttgt ggaaaccgc tctgattgg cccgacgtt tcggttttt ttatatgtg tatcagtgt ttgagcgaa acgtcgcgc gaatcgctt cgacgcatg cgcgactct tttttacat aataataat atatcatat agtgcgacc ccttcctgg cgcgaaaag ccgactcgg ccggcgcgt gcgactgtt tttatattt ataagtcat gtcacaata taaatatta aaagagaaa ttcaatttt attgtctcg ctattattt caaattatg ggcacaaaa ctgggcatt gtagcaaaa acgctgggt ttagatttt tcgagctac acttcttat ccctcgtcc cgtttttac aatgttttc gagtttgga agcgacgcg gacacgtgg tccggcaca aattctaat gccgcattt ttcggttcg gttttgttt cgcataagc gtagtcacg gagaatttt atacattct tgtggtggc atgcatttt tttttcaaa ctgtaggtt agattgtac ttttttatt gttaatgat ttaaattaa aagcgtatg gtgtatgac tactatagc aacctatat gttattgta acgtaataa ataaataaa caaaca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
