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Gene information
General information
TC014882 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9169954..9191106
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014882
    iBeetle screen iB_02343

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Fertility
    egg chamber mostlynot present - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014882 RA
      MNELDSLRQ EAETLKNAI RDARKAACD TSLVQATAS 
LEPIGRVQM RTRRTLRGH LAKIYAMHW GSDSRNLVS 
ASQDGKLIV WDSHTTNKV HAIPLRSSW VMTCAYAPS 
GSYVACGGL DNICSIYSL KTREGNVRV SRELPGHTG 
YLSCCRFLD DNQIVTSSG DMSCALWDI ETGQQVTSF 
LGHTGDVMS LSLSPDMRT FVSGACDAS AKLWDIREG 
LCKQTFPGH ESDINAVTF FPNGFAFAT GSDDATCRL 
FDIRADQEL AMYSHDNII CGITSVAFS KSGRLLLAG 
YDDFNCNVW DSMKTERAG ILAGHDNRV SCLGVTENG 
MAVGTGSWD SFLRIWN
    CDS sequence
    • >TC014882 RA
      atgaacgaa ttggattcc ttgagacaa gaagctgag 
acactcaaa aatgccatc agagatgcc cgtaaagcg 
gcttgcgac acatccttg gtccaggca actgccagc 
cttgaaccc attggaagg gtgcaaatg cgaactaga 
cgcaccctt cgggggcac ttggcaaaa atctacgcc 
atgcattgg ggttccgac agtcgtaac ttggtatcg 
gcctctcag gacggtaaa ctcatcgtc tgggattcc 
cacaccacc aacaaggtt cacgcgatc ccgttgcga 
tcctcatgg gtgatgaca tgcgcgtac gctccatca 
gggagttac gtagcatgc ggaggcctg gacaacatt 
tgctcgatc tacagccta aaaactcgc gaaggcaac 
gtacgagtg agcagagaa ctgcccggt cacaccggg 
tatttgtcg tgctgtcgc ttccttgat gataaccag 
atagtgacc agttcgggc gacatgagt tgcgccctc 
tgggacata gagaccggc cagcaggtc acctcattt 
cttggccac accggcgac gtgatgtcg ctgtcgttg 
tcgccagac atgcgcacg ttcgtttcg ggagcgtgc 
gatgcatcg gcgaagtta tgggatata cgagaagga 
ttatgcaag caaacgttc ccaggacac gagtcggac 
attaatgcc gtcacgttc tttcctaat ggttttgcg 
tttgcgacg ggtagtgat gacgccact tgtaggctc 
ttcgatatc agggccgat caggaacta gccatgtat 
agtcatgat aacattata tgcggaatc accagtgtc 
gcattcagt aaatctgga aggttactg cttgcagga 
tacgacgat ttcaattgt aatgtctgg gattcgatg 
aaaacagaa cgagcaggt atcctagcg ggccacgac 
aatcgagta agctgtcta ggcgtaaca gagaacgga 
atggcggta ggaaccgga tcttgggac agtttccta 
cgcatttgg aactag
    mRNA sequence
    • >TC014882 RA
      cagaaatgg ccgttctga cgtcacgaa tcggggact 
ggaatcgtt accgcaagg gcaccggcg attctcgca 
ccgcaccca agtaggaaa gcgagagga aaattcgcg 
tattaaaaa cattgtagg tgtgaaata ggtgcgcga 
aagtgtcgt taatcgtat gtacggtaa ataaaataa 
tctcacatg ttcataatt agtggacga gtgaatttg 
tatgggtgt aagcggatg tattcgtga ttttgtgag 
aagcaccat aggatagta tagtcgata gtggattgg 
gacagacaa agccgattg gaaaagcgt tcgttgtgc 
catagagag tgttgggca gtatgaacg aattggatt 
ccttgagac aagaagctg agacactca aaaatgcca 
tcagagatg cccgtaaag cggcttgcg acacatcct 
tggtccagg caactgcca gccttgaac ccattggaa 
gggtgcaaa tgcgaacta gacgcaccc ttcgggggc 
acttggcaa aaatctacg ccatgcatt ggggttccg 
acagtcgta acttggtat cggcctctc aggacggta 
aactcatcg tctgggatt cccacacca ccaacaagg 
ttcacgcga tcccgttgc gatcctcat gggtgatga 
catgcgcgt acgctccat cagggagtt acgtagcat 
gcggaggcc tggacaaca tttgctcga tctacagcc 
taaaaactc gcgaaggca acgtacgag tgagcagag 
aactgcccg gtcacaccg ggtatttgt cgtgctgtc 
gcttccttg atgataacc agatagtga ccagttcgg 
gcgacatga gttgcgccc tctgggaca tagagaccg 
gccagcagg tcacctcat ttcttggcc acaccggcg 
acgtgatgt cgctgtcgt tgtcgccag acatgcgca 
cgttcgttt cgggagcgt gcgatgcat cggcgaagt 
tatgggata tacgagaag gattatgca agcaaacgt 
tcccaggac acgagtcgg acattaatg ccgtcacgt 
tctttccta atggttttg cgtttgcga cgggtagtg 
atgacgcca cttgtaggc tcttcgata tcagggccg 
atcaggaac tagccatgt atagtcatg ataacatta 
tatgcggaa tcaccagtg tcgcattca gtaaatctg 
gaaggttac tgcttgcag gatacgacg atttcaatt 
gtaatgtct gggattcga tgaaaacag aacgagcag 
gtatcctag cgggccacg acaatcgag taagctgtc 
taggcgtaa cagagaacg gaatggcgg taggaaccg 
gatcttggg acagtttcc tacgcattt ggaactaga 
cagcgttgc caaaaatta aatgtaatt attcgtatc 
tcacctgca tcacagcat tgatttgtt tttcaaaaa 
taacttcca tgtttcaac aagcattta aaccgtgtc 
gaagttcca agcatttta attattttt tgtattata 
ggattttat ctgttcttg ttctctccg cttatgtag 
tcaccttga tttttataa gagtatgta gcttgtgat 
ttgtttgtt tgtaaggta tctgtattt attattaca 
cgtatttta aagaagaaa aaaattata aataaagca 
gtattttta gttttttct tttttgttt ttctctttc 
gtttttttt tttttcaaa tgtgatgtg ggtgagatt 
gaaccgagt cgaggagtg tattagttc caaaacctt 
tggttagaa tcaagtgca aaacgcaag accaatgtt 
aggaaataa aagtttttg catttttac atgagcgaa 
tttgggttg agtgcgagt ggtggttcc caaaacaaa 
cagaatttt ttacacgaa attattgcc tatttgtcc 
atataaaaa tgcaataat tttgatttt acttctcat 
ggcatatgc tactagtcg gtttaccaa aaaaagcag 
aaaccatgt gccataacc tgaaacttt ccgatgatc 
atagccgtc gacatcaat cagcgtcac aaagtacca 
aattgattt tccgctgtg acgtcataa ccaaatgcg 
tttgtgtaa gaactggca ggttgtaaa aatcaaaca 
gttttgaga tactgcctc aatatttta acctgttaa 
aaacagtgt tccattaat taatttcac acttcataa 
ataaagagg gaaaaacac aaaaaaatc tcatcacgt 
gatcaaatc actcaattg tttcggtta cgaatcacc 
gtttcgttt tgtacatat tgagttcct tattgtatt 
tgcattttt aggatacag tcatgtctt taaataaaa 
taggtgaaa aaaaaagaa aaaatttac gataatata 
atactaaaa ctagcttct gcttgtcat tgattgaat 
gtgaaataa acagtgatg tatgtttac caactcttg 
acacaaaga aactgtttt ataagccaa aaatttcac 
ttatttgac tttgatttc atcgcactg cagtcggcg 
tggcgcatt cagacttcg cggcggatg cgccacgcc 
gcctttttt taaattaaa ttatttata acgtgtgag 
tgcgtgagt ggccggcgc gagtcgagg gcgcctctc 
gcgcctgtg ctcgaactt tgcgcaatc gcaaccaat 
ttacaatta acaaacaag ggatccaac agctcgtcc 
gttgttcga aagggctca tggattggt tgtcgttgt 
ggaaaccgc tctgattgg cccgacgtt tcggttttt 
ttatatgtg tatcagtgt ttgagcgaa acgtcgcgc 
gaatcgctt cgacgcatg cgcgactct tttttacat 
aataataat atatcatat agtgcgacc ccttcctgg 
cgcgaaaag ccgactcgg ccggcgcgt gcgactgtt 
tttatattt ataagtcat gtcacaata taaatatta 
aaagagaaa ttcaatttt attgtctcg ctattattt 
caaattatg ggcacaaaa ctgggcatt gtagcaaaa 
acgctgggt ttagatttt tcgagctac acttcttat 
ccctcgtcc cgtttttac aatgttttc gagtttgga 
agcgacgcg gacacgtgg tccggcaca aattctaat 
gccgcattt ttcggttcg gttttgttt cgcataagc 
gtagtcacg gagaatttt atacattct tgtggtggc 
atgcatttt tttttcaaa ctgtaggtt agattgtac 
ttttttatt gttaatgat ttaaattaa aagcgtatg 
gtgtatgac tactatagc aacctatat gttattgta 
acgtaataa ataaataaa caaaca
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