Status
Please wait ...
Gene information
General information
TC014882 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9169954..9191106
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014882
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02343

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    egg chamber mostlynot present - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014882 RA
      MNELDSLRQ EAETLKNAI RDARKAACD TSLVQATAS 
      LEPIGRVQM RTRRTLRGH LAKIYAMHW GSDSRNLVS 
      ASQDGKLIV WDSHTTNKV HAIPLRSSW VMTCAYAPS 
      GSYVACGGL DNICSIYSL KTREGNVRV SRELPGHTG 
      YLSCCRFLD DNQIVTSSG DMSCALWDI ETGQQVTSF 
      LGHTGDVMS LSLSPDMRT FVSGACDAS AKLWDIREG 
      LCKQTFPGH ESDINAVTF FPNGFAFAT GSDDATCRL 
      FDIRADQEL AMYSHDNII CGITSVAFS KSGRLLLAG 
      YDDFNCNVW DSMKTERAG ILAGHDNRV SCLGVTENG 
      MAVGTGSWD SFLRIWN
    CDS sequence
    • >TC014882 RA
      atgaacgaa ttggattcc ttgagacaa gaagctgag 
      acactcaaa aatgccatc agagatgcc cgtaaagcg 
      gcttgcgac acatccttg gtccaggca actgccagc 
      cttgaaccc attggaagg gtgcaaatg cgaactaga 
      cgcaccctt cgggggcac ttggcaaaa atctacgcc 
      atgcattgg ggttccgac agtcgtaac ttggtatcg 
      gcctctcag gacggtaaa ctcatcgtc tgggattcc 
      cacaccacc aacaaggtt cacgcgatc ccgttgcga 
      tcctcatgg gtgatgaca tgcgcgtac gctccatca 
      gggagttac gtagcatgc ggaggcctg gacaacatt 
      tgctcgatc tacagccta aaaactcgc gaaggcaac 
      gtacgagtg agcagagaa ctgcccggt cacaccggg 
      tatttgtcg tgctgtcgc ttccttgat gataaccag 
      atagtgacc agttcgggc gacatgagt tgcgccctc 
      tgggacata gagaccggc cagcaggtc acctcattt 
      cttggccac accggcgac gtgatgtcg ctgtcgttg 
      tcgccagac atgcgcacg ttcgtttcg ggagcgtgc 
      gatgcatcg gcgaagtta tgggatata cgagaagga 
      ttatgcaag caaacgttc ccaggacac gagtcggac 
      attaatgcc gtcacgttc tttcctaat ggttttgcg 
      tttgcgacg ggtagtgat gacgccact tgtaggctc 
      ttcgatatc agggccgat caggaacta gccatgtat 
      agtcatgat aacattata tgcggaatc accagtgtc 
      gcattcagt aaatctgga aggttactg cttgcagga 
      tacgacgat ttcaattgt aatgtctgg gattcgatg 
      aaaacagaa cgagcaggt atcctagcg ggccacgac 
      aatcgagta agctgtcta ggcgtaaca gagaacgga 
      atggcggta ggaaccgga tcttgggac agtttccta 
      cgcatttgg aactag
    mRNA sequence
    • >TC014882 RA
      cagaaatgg ccgttctga cgtcacgaa tcggggact 
      ggaatcgtt accgcaagg gcaccggcg attctcgca 
      ccgcaccca agtaggaaa gcgagagga aaattcgcg 
      tattaaaaa cattgtagg tgtgaaata ggtgcgcga 
      aagtgtcgt taatcgtat gtacggtaa ataaaataa 
      tctcacatg ttcataatt agtggacga gtgaatttg 
      tatgggtgt aagcggatg tattcgtga ttttgtgag 
      aagcaccat aggatagta tagtcgata gtggattgg 
      gacagacaa agccgattg gaaaagcgt tcgttgtgc 
      catagagag tgttgggca gtatgaacg aattggatt 
      ccttgagac aagaagctg agacactca aaaatgcca 
      tcagagatg cccgtaaag cggcttgcg acacatcct 
      tggtccagg caactgcca gccttgaac ccattggaa 
      gggtgcaaa tgcgaacta gacgcaccc ttcgggggc 
      acttggcaa aaatctacg ccatgcatt ggggttccg 
      acagtcgta acttggtat cggcctctc aggacggta 
      aactcatcg tctgggatt cccacacca ccaacaagg 
      ttcacgcga tcccgttgc gatcctcat gggtgatga 
      catgcgcgt acgctccat cagggagtt acgtagcat 
      gcggaggcc tggacaaca tttgctcga tctacagcc 
      taaaaactc gcgaaggca acgtacgag tgagcagag 
      aactgcccg gtcacaccg ggtatttgt cgtgctgtc 
      gcttccttg atgataacc agatagtga ccagttcgg 
      gcgacatga gttgcgccc tctgggaca tagagaccg 
      gccagcagg tcacctcat ttcttggcc acaccggcg 
      acgtgatgt cgctgtcgt tgtcgccag acatgcgca 
      cgttcgttt cgggagcgt gcgatgcat cggcgaagt 
      tatgggata tacgagaag gattatgca agcaaacgt 
      tcccaggac acgagtcgg acattaatg ccgtcacgt 
      tctttccta atggttttg cgtttgcga cgggtagtg 
      atgacgcca cttgtaggc tcttcgata tcagggccg 
      atcaggaac tagccatgt atagtcatg ataacatta 
      tatgcggaa tcaccagtg tcgcattca gtaaatctg 
      gaaggttac tgcttgcag gatacgacg atttcaatt 
      gtaatgtct gggattcga tgaaaacag aacgagcag 
      gtatcctag cgggccacg acaatcgag taagctgtc 
      taggcgtaa cagagaacg gaatggcgg taggaaccg 
      gatcttggg acagtttcc tacgcattt ggaactaga 
      cagcgttgc caaaaatta aatgtaatt attcgtatc 
      tcacctgca tcacagcat tgatttgtt tttcaaaaa 
      taacttcca tgtttcaac aagcattta aaccgtgtc 
      gaagttcca agcatttta attattttt tgtattata 
      ggattttat ctgttcttg ttctctccg cttatgtag 
      tcaccttga tttttataa gagtatgta gcttgtgat 
      ttgtttgtt tgtaaggta tctgtattt attattaca 
      cgtatttta aagaagaaa aaaattata aataaagca 
      gtattttta gttttttct tttttgttt ttctctttc 
      gtttttttt tttttcaaa tgtgatgtg ggtgagatt 
      gaaccgagt cgaggagtg tattagttc caaaacctt 
      tggttagaa tcaagtgca aaacgcaag accaatgtt 
      aggaaataa aagtttttg catttttac atgagcgaa 
      tttgggttg agtgcgagt ggtggttcc caaaacaaa 
      cagaatttt ttacacgaa attattgcc tatttgtcc 
      atataaaaa tgcaataat tttgatttt acttctcat 
      ggcatatgc tactagtcg gtttaccaa aaaaagcag 
      aaaccatgt gccataacc tgaaacttt ccgatgatc 
      atagccgtc gacatcaat cagcgtcac aaagtacca 
      aattgattt tccgctgtg acgtcataa ccaaatgcg 
      tttgtgtaa gaactggca ggttgtaaa aatcaaaca 
      gttttgaga tactgcctc aatatttta acctgttaa 
      aaacagtgt tccattaat taatttcac acttcataa 
      ataaagagg gaaaaacac aaaaaaatc tcatcacgt 
      gatcaaatc actcaattg tttcggtta cgaatcacc 
      gtttcgttt tgtacatat tgagttcct tattgtatt 
      tgcattttt aggatacag tcatgtctt taaataaaa 
      taggtgaaa aaaaaagaa aaaatttac gataatata 
      atactaaaa ctagcttct gcttgtcat tgattgaat 
      gtgaaataa acagtgatg tatgtttac caactcttg 
      acacaaaga aactgtttt ataagccaa aaatttcac 
      ttatttgac tttgatttc atcgcactg cagtcggcg 
      tggcgcatt cagacttcg cggcggatg cgccacgcc 
      gcctttttt taaattaaa ttatttata acgtgtgag 
      tgcgtgagt ggccggcgc gagtcgagg gcgcctctc 
      gcgcctgtg ctcgaactt tgcgcaatc gcaaccaat 
      ttacaatta acaaacaag ggatccaac agctcgtcc 
      gttgttcga aagggctca tggattggt tgtcgttgt 
      ggaaaccgc tctgattgg cccgacgtt tcggttttt 
      ttatatgtg tatcagtgt ttgagcgaa acgtcgcgc 
      gaatcgctt cgacgcatg cgcgactct tttttacat 
      aataataat atatcatat agtgcgacc ccttcctgg 
      cgcgaaaag ccgactcgg ccggcgcgt gcgactgtt 
      tttatattt ataagtcat gtcacaata taaatatta 
      aaagagaaa ttcaatttt attgtctcg ctattattt 
      caaattatg ggcacaaaa ctgggcatt gtagcaaaa 
      acgctgggt ttagatttt tcgagctac acttcttat 
      ccctcgtcc cgtttttac aatgttttc gagtttgga 
      agcgacgcg gacacgtgg tccggcaca aattctaat 
      gccgcattt ttcggttcg gttttgttt cgcataagc 
      gtagtcacg gagaatttt atacattct tgtggtggc 
      atgcatttt tttttcaaa ctgtaggtt agattgtac 
      ttttttatt gttaatgat ttaaattaa aagcgtatg 
      gtgtatgac tactatagc aacctatat gttattgta 
      acgtaataa ataaataaa caaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY