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Gene information
General information
TC014879 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9247748..9256186
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014879
    iBeetle screen iB_02342

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014879 RA
      MAFFKRSKI PRNNTFYER PKYERQNND AAETDTTDQ 
      NYDSFGKIF MFGEFGPNS KPLKPLQNY DQLLKQHLR 
      NKTRFQDAN FPAERIALT DLPEKVVWR RPREICENA 
      KFIVDGATR FDIDQGLLG DCWFLAAIA NLTTNPKIF 
      EMVVPKNQS FSENYAGIF HFRFWQYGQ WVDIVIDDK 
      LPTINNQLV YLKAGEGNE FWPPLLEKA FAKFYGSYK 
      SIEGGRINE ALEDLCGGL SEVYDTRNP ELYEIIQMS 
      YNKGSFMGC SIPSQNDGN GKRSDGLVV NHAYSITGL 
      YTSQLKNVT IHLIRVRNP WGSVEWNGP WSDNSVCWS 
      LVPKETKQK LWVKREDGE FWMNFTDFT EKFIFIEIC 
      HLNPTFQTN TFQTNNWKV YMFEDSWGP KMAKNQYLI 
      TVRGPKETH VVIGLMQKF RRELGQDEL PLGLLLYQV 
      QKPSKLVEL KRVDPISER QLTARFPLF PGTYCIEPI 
      VVGSSAESS YLLRVYLES GDVIRIN
    CDS sequence
    • >TC014879 RA
      atggcgttc tttaaacgt agcaaaatt ccaagaaat 
      aatactttt tacgaacgt ccgaaatac gaacgccaa 
      aataacgat gcagcagaa acggacaca acagaccaa 
      aactatgac tctttcgga aaaattttt atgtttggt 
      gaattcggt ccaaattct aaaccgcta aaaccacta 
      caaaactat gaccaacta ctaaaacaa catttgcga 
      aataaaaca cgtttccag gatgcaaat tttccagct 
      gaacgcatc gctttgacc gatcttcca gagaaagtg 
      gtttggagg aggcctagg gaaatttgt gaaaacgcc 
      aaatttatc gttgatggg gccacacgt ttcgacatc 
      gaccagggc ctacttggg gactgttgg ttcctcgca 
      gctatcgca aatttgaca acaaatccg aaaattttc 
      gaaatggtt gttccgaaa aatcaaagt ttttcggaa 
      aattatgct ggaattttc cattttaga ttttggcaa 
      tacggccag tgggttgat atagtcatt gatgataaa 
      ttaccaaca atcaacaac cagttggtt tatttaaag 
      gcgggtgag gggaacgag ttttggccc cctttgttg 
      gagaaagct ttcgcaaag ttttacggt tcctataaa 
      agcatcgaa ggtgggaga attaacgaa gctttggag 
      gatttatgt ggtggactt tcggaagtt tacgacacg 
      agaaatccc gaactttac gaaataatc caaatgtcg 
      tacaataaa ggctcattt atggggtgc tcaataccc 
      tcccaaaat gatggaaac gggaaacga tccgatggt 
      ttggtggta aatcatgct tacagtatc accggtttg 
      tatacttca caattaaaa aacgtaacc attcatttg 
      atcagagtg cgaaatccg tggggaagc gttgaatgg 
      aacggtcca tggagtgac aattctgtc tgttggagt 
      ttggtcccc aaggaaact aaacagaaa ttatgggtg 
      aaaagagaa gacggcgaa ttctggatg aacttcacc 
      gattttacg gaaaaattc atcttcatc gagatctgc 
      cacctcaac cccaccttc cagaccaac accttccag 
      accaacaac tggaaggtt tacatgttt gaggactcc 
      tggggccca aaaatggcc aaaaatcag tatttgata 
      accgtaaga gggcccaaa gaaacacac gttgtgatc 
      ggtttaatg caaaaattt cgccgcgag ttgggacaa 
      gacgagttg ccacttgga ctcctcctt tatcaggtc 
      cagaaaccg tcgaaactt gtcgaacta aaacgagtg 
      gacccgatt agcgaacgc cagttaacg gccagattt 
      ccacttttt cctgggaca tactgtata gaaccgatt 
      gttgttggt tctagtgca gaaagcagt tatttgttg 
      agagtttat ctagaaagt ggagatgtg atccgtatt 
      aattaa
    mRNA sequence
    • >TC014879 RA
      tatcagatt taaaatagt ggtgttttt ctatttacg 
      tgaaatggc gttctttaa acgtagcaa aattccaag 
      aaataatac tttttacga acgtccgaa atacgaacg 
      ccaaaataa cgatgcagc agaaacgga cacaacaga 
      ccaaaacta tgactcttt cggaaaaat ttttatgtt 
      tggtgaatt cggtccaaa ttctaaacc gctaaaacc 
      actacaaaa ctatgacca actactaaa acaacattt 
      gcgaaataa aacacgttt ccaggatgc aaattttcc 
      agctgaacg catcgcttt gaccgatct tccagagaa 
      agtggtttg gaggaggcc tagggaaat ttgtgaaaa 
      cgccaaatt tatcgttga tggggccac acgtttcga 
      catcgacca gggcctact tggggactg ttggttcct 
      cgcagctat cgcaaattt gacaacaaa tccgaaaat 
      tttcgaaat ggttgttcc gaaaaatca aagtttttc 
      ggaaaatta tgctggaat tttccattt tagattttg 
      gcaatacgg ccagtgggt tgatatagt cattgatga 
      taaattacc aacaatcaa caaccagtt ggtttattt 
      aaaggcggg tgaggggaa cgagttttg gcccccttt 
      gttggagaa agctttcgc aaagtttta cggttccta 
      taaaagcat cgaaggtgg gagaattaa cgaagcttt 
      ggaggattt atgtggtgg actttcgga agtttacga 
      cacgagaaa tcccgaact ttacgaaat aatccaaat 
      gtcgtacaa taaaggctc atttatggg gtgctcaat 
      accctccca aaatgatgg aaacgggaa acgatccga 
      tggtttggt ggtaaatca tgcttacag tatcaccgg 
      tttgtatac ttcacaatt aaaaaacgt aaccattca 
      tttgatcag agtgcgaaa tccgtgggg aagcgttga 
      atggaacgg tccatggag tgacaattc tgtctgttg 
      gagtttggt ccccaagga aactaaaca gaaattatg 
      ggtgaaaag agaagacgg cgaattctg gatgaactt 
      caccgattt tacggaaaa attcatctt catcgagat 
      ctgccacct caaccccac cttccagac caacacctt 
      ccagaccaa caactggaa ggtttacat gtttgagga 
      ctcctgggg cccaaaaat ggccaaaaa tcagtattt 
      gataaccgt aagagggcc caaagaaac acacgttgt 
      gatcggttt aatgcaaaa atttcgccg cgagttggg 
      acaagacga gttgccact tggactcct cctttatca 
      ggtccagaa accgtcgaa acttgtcga actaaaacg 
      agtggaccc gattagcga acgccagtt aacggccag 
      atttccact ttttcctgg gacatactg tatagaacc 
      gattgttgt tggttctag tgcagaaag cagttattt 
      gttgagagt ttatctaga aagtggaga tgtgatccg 
      tattaatta attgaaact ggttttatt tttatttaa 
      tatattgtt ttattgtat tataattat attcaagaa 
      ttaattata cggcgctat gcataataa ttggtcaaa 
      aaatgtgaa cgcatttac ttagttatt atttattct 
      gaaagtaac agtgaggca atcagtgga agtatttca 
      ttagtctta caataaata tgttgttaa acg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY