Status
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Gene information
General information
TC034748 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9505395..9520481
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034748
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02341

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034748 RA
      MGLGATLKW LVLAHVLAA ALAMAYVSS CSSGTCGQN 
      AQCSIIGGR PVCSCFKGY LGDPITYCK RAECLDNSE 
      CRGHLTCRS GRCIDPCDG TCGANALCT ARNHLPVCT 
      CPPGYTGDP FSHCRRFDP SELCHPSPC GANTHCEVV 
      NETPTCKCL PGYHGSPIS GCRHECDSD SECGPSMAC 
      IEFKCQNPC SQCGKNAEC DVRNHRPVC KCPKNYFGN 
      PLVSCQPEC YGDRDCPAG RPACFYGIC KNPCDGVCG 
      VGANCELRG LTPICSCPR DMTGDPFIH CRPFDKRDL 
      CEPNPCGDN ARCEPGHDR TGKERPVCT CHPGYVGDP 
      LVSCRPGEC TEDSHCPDS KACIDYKCQ NPCVGQCGV 
      NANCNPRRH IAVCTCAEG FNGDALTQC HRIQGGT
    • >TC034748 RB
      MGLGATLKW LVLAHVLAA ALGEPSYYH QRGYPYYTN 
      SYTYYTNPF RQYYNYYNS PAYTKYRSW DNYYNYKSY 
      SRPTSYDDD TVYVTDMYG NTRASSARS LRAMAYVSS 
      CSSGTCGQN AQCSIIGGR PVCSCFKGY LGDPITYCK 
      RAECLDNSE CRGHLTCRS GRCIDPCDG TCGANALCT 
      ARNHLPVCT CPPGYTGDP FSHCRRFDP SELCHPSPC 
      GANTHCEVV NETPTCKCL PGYHGSPIS GCRHECDSD 
      SECGPSMAC IEFKCQNPC SQCGKNAEC DVRNHRPVC 
      KCPKNYFGN PLVSCQPEC YGDRDCPAG RPACFYGIC 
      KNPCDGVCG VGANCELRG LTPICSCPR DMTGDPFIH 
      CRPFDKRDL CEPNPCGDN ARCEPGHDR TGKERPVCT 
      CHPGYVGDP LVSCRPGEC TEDSHCPDS KACIDYKCQ 
      NPCVGQCGV NANCNPRRH IAVCTCAEG FNGDALTQC 
      HRIQGGT
    CDS sequence
    • >TC034748 RA
      atggggttg ggtgcgact ttgaagtgg ctggttctg 
      gcccatgtg ctggctgca gccctagcc atggcctac 
      gtttcgtcg tgttcaagc ggcacttgc ggccaaaac 
      gcccaatgt tccatcatt ggaggacgt ccagtgtgc 
      tcctgcttc aaagggtat cttggagac cccatcact 
      tactgcaaa agggccgaa tgtctcgac aattccgaa 
      tgtcggggg catttgaca tgtcgcagt gggcgttgc 
      attgaccct tgcgatggt acttgcgga gctaatgcc 
      ctttgtacc gccagaaac cacctcccg gtgtgcaca 
      tgccctccg ggttataca ggagaccct ttttcgcac 
      tgccgacgt ttcgacccc tctgaatta tgccaccca 
      agcccatgt ggtgccaac acccattgc gaagtggtc 
      aacgagacg cccacctgc aagtgcctt ccggggtac 
      catggctca ccgatcagt gggtgtcgc catgagtgc 
      gacagtgac agcgaatgt gggccttct atggcttgt 
      atcgagttt aaatgtcaa aatccgtgt tcacaatgt 
      ggcaaaaat gccgaatgt gatgtcagg aatcaccga 
      ccggtttgc aaatgtccg aaaaattat ttcgggaat 
      cccctggtg tcctgccaa cctgagtgt tacggagat 
      agggattgt ccagcagga aggccagct tgcttctat 
      gggatttgt aaaaatccc tgcgatgga gtctgtgga 
      gttggggcc aattgtgag ttaagggga ttgactcca 
      atttgctcc tgtccgagg gatatgacg ggagatccc 
      ttcattcac tgccggccg ttcgacaaa cgtgactta 
      tgtgaacca aacccttgt ggggacaac gcgcgttgt 
      gagccaggc cacgaccga acaggaaaa gaacgtcca 
      gtctgcacc tgccatcca ggttacgta ggagacccc 
      ttggtgtcc tgtcgtcct ggcgaatgc accgaggac 
      tcccactgt cccgattcc aaagcctgc atcgattac 
      aaatgccaa aatccgtgt gtagggcaa tgtggggtc 
      aacgctaat tgtaatcct aggagacat attgctgtg 
      tgtacgtgt gccgaaggg ttcaatggg gacgctctg 
      actcagtgc cataggatt caagggggg acttaa
    • >TC034748 RB
      atggggttg ggtgcgact ttgaagtgg ctggttctg 
      gcccatgtg ctggctgca gccctaggt gagccttca 
      tactaccac caaagaggg tacccgtac tacacaaac 
      agttacact tactacacc aatccgttc agacagtat 
      tacaattat tacaacagt ccggcttat actaagtac 
      agaagttgg gacaattat tacaattac aaaagctac 
      agccgaccg acgagctat gatgatgat acggtttat 
      gttactgat atgtatggg aacaccaga gcgtcgtcg 
      gccaggagt ttgagagcc atggcctac gtttcgtcg 
      tgttcaagc ggcacttgc ggccaaaac gcccaatgt 
      tccatcatt ggaggacgt ccagtgtgc tcctgcttc 
      aaagggtat cttggagac cccatcact tactgcaaa 
      agggccgaa tgtctcgac aattccgaa tgtcggggg 
      catttgaca tgtcgcagt gggcgttgc attgaccct 
      tgcgatggt acttgcgga gctaatgcc ctttgtacc 
      gccagaaac cacctcccg gtgtgcaca tgccctccg 
      ggttataca ggagaccct ttttcgcac tgccgacgt 
      ttcgacccc tctgaatta tgccaccca agcccatgt 
      ggtgccaac acccattgc gaagtggtc aacgagacg 
      cccacctgc aagtgcctt ccggggtac catggctca 
      ccgatcagt gggtgtcgc catgagtgc gacagtgac 
      agcgaatgt gggccttct atggcttgt atcgagttt 
      aaatgtcaa aatccgtgt tcacaatgt ggcaaaaat 
      gccgaatgt gatgtcagg aatcaccga ccggtttgc 
      aaatgtccg aaaaattat ttcgggaat cccctggtg 
      tcctgccaa cctgagtgt tacggagat agggattgt 
      ccagcagga aggccagct tgcttctat gggatttgt 
      aaaaatccc tgcgatgga gtctgtgga gttggggcc 
      aattgtgag ttaagggga ttgactcca atttgctcc 
      tgtccgagg gatatgacg ggagatccc ttcattcac 
      tgccggccg ttcgacaaa cgtgactta tgtgaacca 
      aacccttgt ggggacaac gcgcgttgt gagccaggc 
      cacgaccga acaggaaaa gaacgtcca gtctgcacc 
      tgccatcca ggttacgta ggagacccc ttggtgtcc 
      tgtcgtcct ggcgaatgc accgaggac tcccactgt 
      cccgattcc aaagcctgc atcgattac aaatgccaa 
      aatccgtgt gtagggcaa tgtggggtc aacgctaat 
      tgtaatcct aggagacat attgctgtg tgtacgtgt 
      gccgaaggg ttcaatggg gacgctctg actcagtgc 
      cataggatt caagggggg acttaa
    mRNA sequence
    • >TC034748 RA
      gtatatttt tcaccttag tgattcctt tttccacca 
      ttgtcaagc gaccatttc tcgaccgga cttgtagtt 
      gttgttgtg attttaagt gaggtggac tttgttgta 
      tttttcatt aaaaaaaaa ttagatggg gttgggtgc 
      gactttgaa gtggctggt tctggccca tgtgctggc 
      tgcagccct agccatggc ctacgtttc gtcgtgttc 
      aagcggcac ttgcggcca aaacgccca atgttccat 
      cattggagg acgtccagt gtgctcctg cttcaaagg 
      gtatcttgg agaccccat cacttactg caaaagggc 
      cgaatgtct cgacaattc cgaatgtcg ggggcattt 
      gacatgtcg cagtgggcg ttgcattga cccttgcga 
      tggtacttg cggagctaa tgccctttg taccgccag 
      aaaccacct cccggtgtg cacatgccc tccgggtta 
      tacaggaga ccctttttc gcactgccg acgtttcga 
      cccctctga attatgcca cccaagccc atgtggtgc 
      caacaccca ttgcgaagt ggtcaacga gacgcccac 
      ctgcaagtg ccttccggg gtaccatgg ctcaccgat 
      cagtgggtg tcgccatga gtgcgacag tgacagcga 
      atgtgggcc ttctatggc ttgtatcga gtttaaatg 
      tcaaaatcc gtgttcaca atgtggcaa aaatgccga 
      atgtgatgt caggaatca ccgaccggt ttgcaaatg 
      tccgaaaaa ttatttcgg gaatcccct ggtgtcctg 
      ccaacctga gtgttacgg agataggga ttgtccagc 
      aggaaggcc agcttgctt ctatgggat ttgtaaaaa 
      tccctgcga tggagtctg tggagttgg ggccaattg 
      tgagttaag gggattgac tccaatttg ctcctgtcc 
      gagggatat gacgggaga tcccttcat tcactgccg 
      gccgttcga caaacgtga cttatgtga accaaaccc 
      ttgtgggga caacgcgcg ttgtgagcc aggccacga 
      ccgaacagg aaaagaacg tccagtctg cacctgcca 
      tccaggtta cgtaggaga ccccttggt gtcctgtcg 
      tcctggcga atgcaccga ggactccca ctgtcccga 
      ttccaaagc ctgcatcga ttacaaatg ccaaaatcc 
      gtgtgtagg gcaatgtgg ggtcaacgc taattgtaa 
      tcctaggag acatattgc tgtgtgtac gtgtgccga 
      agggttcaa tggggacgc tctgactca gtgccatag 
      gattcaagg ggggactta aatcactgc caaataacg 
      tgtaatttt atttaacga aacaataaa cacttcttc 
      taact
    • >TC034748 RB
      gtatatttt tcaccttag tgattcctt tttccacca 
      ttgtcaagc gaccatttc tcgaccgga cttgtagtt 
      gttgttgtg attttaagt gaggtggac tttgttgta 
      tttttcatt aaaaaaaaa ttagatggg gttgggtgc 
      gactttgaa gtggctggt tctggccca tgtgctggc 
      tgcagccct aggtgagcc ttcatacta ccaccaaag 
      agggtaccc gtactacac aaacagtta cacttacta 
      caccaatcc gttcagaca gtattacaa ttattacaa 
      cagtccggc ttatactaa gtacagaag ttgggacaa 
      ttattacaa ttacaaaag ctacagccg accgacgag 
      ctatgatga tgatacggt ttatgttac tgatatgta 
      tgggaacac cagagcgtc gtcggccag gagtttgag 
      agccatggc ctacgtttc gtcgtgttc aagcggcac 
      ttgcggcca aaacgccca atgttccat cattggagg 
      acgtccagt gtgctcctg cttcaaagg gtatcttgg 
      agaccccat cacttactg caaaagggc cgaatgtct 
      cgacaattc cgaatgtcg ggggcattt gacatgtcg 
      cagtgggcg ttgcattga cccttgcga tggtacttg 
      cggagctaa tgccctttg taccgccag aaaccacct 
      cccggtgtg cacatgccc tccgggtta tacaggaga 
      ccctttttc gcactgccg acgtttcga cccctctga 
      attatgcca cccaagccc atgtggtgc caacaccca 
      ttgcgaagt ggtcaacga gacgcccac ctgcaagtg 
      ccttccggg gtaccatgg ctcaccgat cagtgggtg 
      tcgccatga gtgcgacag tgacagcga atgtgggcc 
      ttctatggc ttgtatcga gtttaaatg tcaaaatcc 
      gtgttcaca atgtggcaa aaatgccga atgtgatgt 
      caggaatca ccgaccggt ttgcaaatg tccgaaaaa 
      ttatttcgg gaatcccct ggtgtcctg ccaacctga 
      gtgttacgg agataggga ttgtccagc aggaaggcc 
      agcttgctt ctatgggat ttgtaaaaa tccctgcga 
      tggagtctg tggagttgg ggccaattg tgagttaag 
      gggattgac tccaatttg ctcctgtcc gagggatat 
      gacgggaga tcccttcat tcactgccg gccgttcga 
      caaacgtga cttatgtga accaaaccc ttgtgggga 
      caacgcgcg ttgtgagcc aggccacga ccgaacagg 
      aaaagaacg tccagtctg cacctgcca tccaggtta 
      cgtaggaga ccccttggt gtcctgtcg tcctggcga 
      atgcaccga ggactccca ctgtcccga ttccaaagc 
      ctgcatcga ttacaaatg ccaaaatcc gtgtgtagg 
      gcaatgtgg ggtcaacgc taattgtaa tcctaggag 
      acatattgc tgtgtgtac gtgtgccga agggttcaa 
      tggggacgc tctgactca gtgccatag gattcaagg 
      ggggactta aatcactgc caaataacg tgtaatttt 
      atttaacga aacaataaa cacttcttc taact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY