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Gene information
General information
TC014861 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9620315..9628056
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014861
      iBeetle screen iB_02340

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen segment anterior potentiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      abdomen constricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014861 RA
        MLIKIIVIC SALAAFAEP QHKVHNIIL YPEKYSWCQ 
        TTPIQQVVA SPGYESVTI HNNVCVGAC YSYSIPSTQ 
        PAEPGELLG PYCDSCQPV ETKCYHVTL HADGKNTEG 
        PKTFQKRVQ IILKCSCMS CEKYHREDC EITDQTTLE 
        LPQDLFTNK SQEPKPEEP PELLDLVSN KKKRPIHNF 
        DIANTTTEQ RYQLNAKLI NLLKSIQNE GEDSNISYD 
        KEQLRELLK LIEGSEDEL SDKNLMEFV NFVNVHNSE 
        DLELDLSRL KDVLTNFQL LEKHRDFGF GQSQKNNAK 
        IEQDLKKYN LPKSFGLEE SHRHNHHLG DEVHVGLDV 
        GHLVKGPHG SMVLSPNLK VEEKLNIDS DHLKPNHDG 
        VVITYENHR KGNDILTN
      CDS sequence
      • >TC014861 RA
        atgttgatt aaaattatc gtgatttgt tcggctttg 
        gcggctttt gccgaacca caacacaag gtccataat 
        attattttg taccctgaa aaatactcc tggtgccaa 
        accaccccc attcaacag gtggtggca tcgccagga 
        tacgaatcc gtcacaatt cacaacaat gtgtgcgtg 
        ggggcctgc tacagctat agcattcct agcacacag 
        ccggccgaa cccggggaa ctactgggg ccatactgc 
        gacagctgc caaccggta gaaacaaag tgctaccat 
        gtcacccta cacgccgat ggaaaaaac accgagggc 
        cccaaaacc ttccaaaaa cgcgtccaa atcatcctc 
        aagtgttca tgcatgtcg tgcgaaaaa taccaccgt 
        gaagattgt gaaattaca gatcaaaca actttggaa 
        ttaccccag gacctcttc actaacaaa agccaagaa 
        ccgaaaccc gaggaaccc ccagaatta ctcgacctt 
        gtttcaaat aagaaaaaa cgtccaata cataatttc 
        gatattgcc aacactaca accgagcag cgctaccaa 
        ctcaacgcc aaattgata aatttattg aaaagtatt 
        caaaacgaa ggcgaggac agtaatata agctacgat 
        aaagagcag ttgagagag ttgttgaag ttgattgaa 
        gggtctgag gatgagctg agtgataaa aatttgatg 
        gagtttgtg aacttcgtc aatgtgcac aattcggag 
        gatttggag ctggatttg agtcggttg aaggacgtt 
        ttgaccaat ttccaactt ttggagaaa catagagac 
        tttggtttt ggtcagagt cagaaaaac aacgccaaa 
        atcgagcaa gatttgaag aaatataat ctaccgaaa 
        agtttcggt ttggaggag tcccacaga cacaaccac 
        cacctgggg gacgaggtg cacgtgggc ctggacgtg 
        gggcacctc gtcaagggc ccccatggc agtatggtc 
        ctctccccg aacctcaaa gtcgaggag aagctaaac 
        attgactcg gatcatctc aaaccgaac cacgacggc 
        gttgttatc acgtatgag aatcacaga aagggcaac 
        gatattcta acaaattaa
      mRNA sequence
      • >TC014861 RA
        aacaagtag attctcagt tgtttcgtg acctttcac 
        tagctatct tgatttaaa tgttgtaag gagaacggt 
        tcgcgtcgc aaggaaaat cgcagcgtt tgtggtcca 
        cagccagct ttggatgcg tgtaccatg ttgattaaa 
        attatcgtg atttgttcg gctttggcg gcttttgcc 
        gaaccacaa cacaaggtc cataatatt attttgtac 
        cctgaaaaa tactcctgg tgccaaacc acccccatt 
        caacaggtg gtggcatcg ccaggatac gaatccgtc 
        acaattcac aacaatgtg tgcgtgggg gcctgctac 
        agctatagc attcctagc acacagccg gccgaaccc 
        ggggaacta ctggggcca tactgcgac agctgccaa 
        ccggtagaa acaaagtgc taccatgtc accctacac 
        gccgatgga aaaaacacc gagggcccc aaaaccttc 
        caaaaacgc gtccaaatc atcctcaag tgttcatgc 
        atgtcgtgc gaaaaatac caccgtgaa gattgtgaa 
        attacagat caaacaact ttggaatta ccccaggac 
        ctcttcact aacaaaagc caagaaccg aaacccgag 
        gaaccccca gaattactc gaccttgtt tcaaataag 
        aaaaaacgt ccaatacat aatttcgat attgccaac 
        actacaacc gagcagcgc taccaactc aacgccaaa 
        ttgataaat ttattgaaa agtattcaa aacgaaggc 
        gaggacagt aatataagc tacgataaa gagcagttg 
        agagagttg ttgaagttg attgaaggg tctgaggat 
        gagctgagt gataaaaat ttgatggag tttgtgaac 
        ttcgtcaat gtgcacaat tcggaggat ttggagctg 
        gatttgagt cggttgaag gacgttttg accaatttc 
        caacttttg gagaaacat agagacttt ggttttggt 
        cagagtcag aaaaacaac gccaaaatc gagcaagat 
        ttgaagaaa tataatcta ccgaaaagt ttcggtttg 
        gaggagtcc cacagacac aaccaccac ctgggggac 
        gaggtgcac gtgggcctg gacgtgggg cacctcgtc 
        aagggcccc catggcagt atggtcctc tccccgaac 
        ctcaaagtc gaggagaag ctaaacatt gactcggat 
        catctcaaa ccgaaccac gacggcgtt gttatcacg 
        tatgagaat cacagaaag ggcaacgat attctaaca 
        aattaattt cgcaaattg gacgtttcg agattctag 
        tcgtgtgaa agaaaaaag atttgccgt cgctctagt 
        attagaaaa tcttaaata cgtaacttt actgtctct 
        tcaaacata cagctttaa gtagtacaa aaaaaagaa 
        tttttttgg acggtcggc ctctgaaaa aatctaaag 
        tgtacagta caataattt tagcgtcta ctaatccgg 
        gctaacaaa attattcga tagaatctg tggtatttt 
        aagctcttt tgcgtaagt tatcgaaat tgtaaaggc 
        tgtgtgctt tttttagtc aaaatattt ttacttttc 
        tttactttt gttatcata gttttatat atttttatg 
        tactaatct gttttaacg aaatataat atattccat 
        ttc
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY