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Gene information
General information
TC014857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid43: 4378061..4381455
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014857
    iBeetle screen iB_02339
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014857 RA
      MSRPEHLAP PEIFYNEDE ARKYTQNSR IIDIQMQMS 
      ERAVELLLL PEDQPCYLL DIGCGSGLS GSVLEDQGH 
      FWVGMDISK AMLDVALER EVEGDVLLS DMGQGCPFR 
      AGSFDGAIS ISALQWLCN ADKKSHNPV QRIYKFFSS 
      LYACLSRSA RAVFQFYPE NSEQMELVT AQAMKAGFY 
      GGVVVDFPN STKAKKFFL VLMTGGNMP LPKGLGTEE 
      ESQGVSYIS RREHAKKAR GKPLKNSRD WILEKKERR 
      RRQMKDVRA DSKYTGRKR SGRF
    CDS sequence
    • >TC014857 RA
      atgtcgcgt cctgagcac ttggcgcca cccgaaatc 
      ttttacaac gaagatgag gcccggaag tacactcaa 
      aattctcga attattgat attcaaatg cagatgagt 
      gagcgtgca gttgagctc cttttgttg cctgaagac 
      cagccgtgt tatttgctt gatataggt tgcgggtcg 
      gggcttagc gggagcgtc cttgaggat caggggcac 
      ttctgggtc ggtatggac atatccaag gccatgtta 
      gatgtggcc cttgaaagg gaggtcgag ggggacgtt 
      ttgttgtca gacatgggg caagggtgc ccctttcgg 
      gccgggtcg ttcgatggc gcgattagc ataagcgcg 
      ttgcagtgg ttgtgcaat gctgataaa aagtcgcac 
      aatcccgtt caaaggatt tacaagttt tttagcagt 
      ctttatgcg tgtctgagc cggtcagca cgggccgtt 
      tttcagttt tatcccgaa aatagcgaa caaatggag 
      ttggttacg gcacaagca atgaaagcg gggttttat 
      ggcggggtt gttgttgat tttccgaat tcaactaaa 
      gcgaaaaag ttttttttg gtcttgatg acaggggga 
      aatatgccg ttgccgaag ggattgggg acagaggag 
      gaatcacaa ggtgtttca tacatttcg aggcgagag 
      catgccaaa aaagcgcgt ggaaaaccg ttaaagaat 
      agtcgtgac tggattttg gagaaaaaa gaacgtcga 
      cgaagacaa atgaaggac gttagggct gattcgaag 
      tacaccggg cggaaacga agcggcaga ttttga
    mRNA sequence
    • >TC014857 RA
      gtcattttc accctaact aacctcaat tctgattac 
      tttttaatc ctaagatag cacatgtgt tgttattgt 
      tttggtcct aaataaaca ccaatgtcg cgtcctgag 
      cacttggcg ccacccgaa atcttttac aacgaagat 
      gaggcccgg aagtacact caaaattct cgaattatt 
      gatattcaa atgcagatg agtgagcgt gcagttgag 
      ctccttttg ttgcctgaa gaccagccg tgttatttg 
      cttgatata ggttgcggg tcggggctt agcgggagc 
      gtccttgag gatcagggg cacttctgg gtcggtatg 
      gacatatcc aaggccatg ttagatgtg gcccttgaa 
      agggaggtc gagggggac gttttgttg tcagacatg 
      gggcaaggg tgccccttt cgggccggg tcgttcgat 
      ggcgcgatt agcataagc gcgttgcag tggttgtgc 
      aatgctgat aaaaagtcg cacaatccc gttcaaagg 
      atttacaag ttttttagc agtctttat gcgtgtctg 
      agccggtca gcacgggcc gtttttcag ttttatccc 
      gaaaatagc gaacaaatg gagttggtt acggcacaa 
      gcaatgaaa gcggggttt tatggcggg gttgttgtt 
      gattttccg aattcaact aaagcgaaa aagtttttt 
      ttggtcttg atgacaggg ggaaatatg ccgttgccg 
      aagggattg gggacagag gaggaatca caaggtgtt 
      tcatacatt tcgaggcga gagcatgcc aaaaaagcg 
      cgtggaaaa ccgttaaag aatagtcgt gactggatt 
      ttggagaaa aaagaacgt cgacgaaga caaatgaag 
      gacgttagg gctgattcg aagtacacc gggcggaaa 
      cgaagcggc agattttga tttatataa aatgtacca 
      taaataata cgaaaaata aaagagatt acttaat