Status
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Gene information
General information
TC014821 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450245.1: 61063..72069
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014821
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02337

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 12.5% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 37.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014821 RA
      MAPNLLGNS TLFLAETNS AEPIQIISK PGLQDVLPQ 
      VKPQISSRS SVSQYRWQI VWRNVLIFI YLHIAGIYG 
      LYYAIAQAQ WKTLLWGYL VILASGIGV TAGAHRLWA 
      HRTYKAKLP LRIYLAFCQ TVALQNDIY EWVRDHRVH 
      HKFTDTDAD PHNSNRGFF FSHMGWLLV KKHKDVFVK 
      GKTVDMSDV EADPVVRFQ RKYYIILTP ILTFVFPAI 
      VPWYFWNET PTVCFYSVA IFRYILTLH GTWLVNSAA 
      HIWGYRPYD KNINATENK SVSILAFGE GWHNYHHVF 
      PWDYKAAEL GNYRMNFTT AFLDLMSKI GQAYDLKTV 
      SVDMINKRR KRTGDGTGL VDEELLENE DKHHHHHDD 
      SIWGWGDKD MKQDDMDMV QVHNPSREK FD
    CDS sequence
    • >TC014821 RA
      atggctcct aatttgcta ggaaattca acgttattt 
      ctcgctgaa acaaattca gccgaacca atacagatt 
      attagtaaa ccaggactg caagatgta ctaccgcaa 
      gtaaagcca caaatttcc agcagatct tctgtgtcc 
      caatataga tggcagatt gtatggaga aacgttttg 
      atatttata tacttgcac attgctggt atttatgga 
      ttatattat gctattgct caagcgcaa tggaaaact 
      cttctctgg ggatattta gttattcta gcatcagga 
      attggcgta acagcagga gcgcacaga ctgtgggcc 
      catcgcaca tacaaagct aaacttcca ttacgaatt 
      tacttagct ttttgtcaa actgttgct ttacaaaat 
      gacatctac gaatgggtg cgagaccat cgagttcat 
      cacaaattc acagataca gacgctgat cctcacaac 
      tctaatcga ggattcttt ttctcacac atgggatgg 
      ttattggtt aaaaaacac aaggatgtt ttcgtcaag 
      ggcaaaact gtggatatg agtgatgtg gaagccgat 
      ccggtggta cggtttcaa agaaaatac tacataatt 
      ctaactcca attttaaca tttgttttt cctgctatc 
      gttccttgg tacttctgg aacgaaact cccactgtt 
      tgcttctat agtgtcgcc atctttaga tatattctc 
      acacttcat gggacttgg ctggtaaat agtgcagct 
      catatttgg ggctataga ccctatgac aaaaacata 
      aatgccaca gaaaacaaa agcgtttca attctcgct 
      ttcggtgaa ggatggcac aactatcat catgtcttc 
      ccttgggat tacaaagcc gcagaatta ggaaactac 
      aggatgaat tttactacg gcttttctg gatttgatg 
      tcaaaaatt ggacaggct tatgatttg aaaaccgtg 
      tcagttgat atgatcaat aagagaaga aagagaact 
      ggagatggt actggattg gttgacgag gaattgcta 
      gaaaatgaa gataaacat caccaccat catgatgac 
      agcatctgg ggatggggt gataaagat atgaaacaa 
      gatgacatg gatatggta caagtgcat aatccaagt 
      agagagaaa tttgattaa
    mRNA sequence
    • >TC014821 RA
      tttgttcaa attgtcact aaatcactc atctgcatt 
      tttctcaaa agtaaattt agtaaaaat aaacttctt 
      taaaaagtt ttagtaaca ttttcattc gacgtgaaa 
      gtgagaaaa tacattgaa aaaaacgat ggctcctaa 
      tttgctagg aaattcaac gttatttct cgctgaaac 
      aaattcagc cgaaccaat acagattat tagtaaacc 
      aggactgca agatgtact accgcaagt aaagccaca 
      aatttccag cagatcttc tgtgtccca atatagatg 
      gcagattgt atggagaaa cgttttgat atttatata 
      cttgcacat tgctggtat ttatggatt atattatgc 
      tattgctca agcgcaatg gaaaactct tctctgggg 
      atatttagt tattctagc atcaggaat tggcgtaac 
      agcaggagc gcacagact gtgggccca tcgcacata 
      caaagctaa acttccatt acgaattta cttagcttt 
      ttgtcaaac tgttgcttt acaaaatga catctacga 
      atgggtgcg agaccatcg agttcatca caaattcac 
      agatacaga cgctgatcc tcacaactc taatcgagg 
      attcttttt ctcacacat gggatggtt attggttaa 
      aaaacacaa ggatgtttt cgtcaaggg caaaactgt 
      ggatatgag tgatgtgga agccgatcc ggtggtacg 
      gtttcaaag aaaatacta cataattct aactccaat 
      tttaacatt tgtttttcc tgctatcgt tccttggta 
      cttctggaa cgaaactcc cactgtttg cttctatag 
      tgtcgccat ctttagata tattctcac acttcatgg 
      gacttggct ggtaaatag tgcagctca tatttgggg 
      ctatagacc ctatgacaa aaacataaa tgccacaga 
      aaacaaaag cgtttcaat tctcgcttt cggtgaagg 
      atggcacaa ctatcatca tgtcttccc ttgggatta 
      caaagccgc agaattagg aaactacag gatgaattt 
      tactacggc ttttctgga tttgatgtc aaaaattgg 
      acaggctta tgatttgaa aaccgtgtc agttgatat 
      gatcaataa gagaagaaa gagaactgg agatggtac 
      tggattggt tgacgagga attgctaga aaatgaaga 
      taaacatca ccaccatca tgatgacag catctgggg 
      atggggtga taaagatat gaaacaaga tgacatgga 
      tatggtaca agtgcataa tccaagtag agagaaatt 
      tgattaaaa ttgagtata tttgctgtg atgtttggt 
      taccagtaa tatttgatt ctcttgtta tttattgtt 
      atatcttta aattgtatc tatttataa tttattttt 
      tctggtggc tttatgtgt taaatattt cgccatgtt 
      gtcggtact ttgtcaagc tttatgtgt ttaaataaa 
      ttgtaacct acttgtttt aattttcat aagcctaaa 
      tctgtgtta tgtttgtca ttgtaattt ctaagttaa 
      agtatatat aaataaatg caatacttt tca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY