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Gene information
General information
TC014815 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15431668..15436262
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014815
    iBeetle screen iB_02336

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014815 RA
      MKNLKSVSV QVIDSDIAL LLPNGVYEI KQMEPKSSR 
      LRRIGIFCV ALALFGIVG FGYFCPDKV CALSNKEAS 
      ISLEDNLGK SLSQASSKV GLSYEDMLN DDFFDMDSH 
      DVMVFLHMQ KTGGTSFGK HLVHDLDLK RPCTCPLTR 
      KRCYCFRPH SNQSWLFSR YTTGWKCGL HADWTELTS 
      CVDEKLDEL EGNVVKRRY FYITLLREP VARYLSEFR 
      HVQRGATWK GSRHFCRGR PATDDEIPP CYEGPDWGG 
      VALDEFIEC ESNLAVNRQ TRMLADLEL VGCYDKSYM 
      SKADRDRTM LISAKKNLE SMAFFGLTE YQKMSQYIF 
      EETFNLRFA VPFEQNNAT VSSSTMPTL TDEQAAKIV 
      ALNSLDIEL YKYAKELLF KRFERLRAR DSDFNKRYS 
      HLGELTARS GVTEFDWDR LVEDATVSN
    CDS sequence
    • >TC014815 RA
      atgaagaat ttgaagagc gtgtcggtg caagtaatt 
      gacagcgac atagccttg ctgttgccg aatggtgtg 
      tatgaaata aaacaaatg gagccaaaa tcttccaga 
      ttgagacgg atagggatt ttctgtgtg gctctggcc 
      ctattcggc atcgtaggt ttcggctac ttttgtcca 
      gacaaagta tgtgccctt agtaataaa gaagcatcc 
      atatcacta gaagacaat ttagggaaa tcgctctct 
      caagctagc agtaaagta ggtttaagt tatgaggat 
      atgttgaac gatgatttt ttcgatatg gattcacat 
      gatgtaatg gtgtttctc cacatgcag aagactggt 
      ggcacctcc tttggcaaa cacctcgtc cacgacctg 
      gacttgaaa cgtccatgc acttgtcca ctaacccgg 
      aagcgttgc tattgtttc cgtccgcat tccaaccaa 
      tcgtggctc ttcagccgc tacaccacc ggctggaaa 
      tgcggcctc catgccgac tggaccgaa ctaaccagt 
      tgtgtagac gaaaaactc gacgaactt gaaggtaac 
      gtcgtcaaa agacgctac ttctacatc acccttctt 
      cgagagccc gttgctcga tatctgagc gaattcaga 
      catgtccaa cgtggggcc acctggaag ggctcccga 
      cacttctgc agagggcgt ccagccacc gacgacgaa 
      ataccccca tgttacgag ggccccgat tggggcgga 
      gtcgccctt gatgagttc atcgaatgc gagagtaat 
      ttggcagtg aatcgccaa acgcgaatg ttggcagat 
      ctcgaattg gtgggttgt tatgataag agttacatg 
      tcgaaggcg gatagagac aggacgatg ttgataagc 
      gcgaaaaaa aatctcgaa tcgatggcg ttttttggt 
      ttaacagaa taccaaaaa atgtcgcag tatattttc 
      gaggagact tttaatttg aggtttgcg gtgccgttt 
      gagcaaaat aatgcgacg gtttcgagt tcaacaatg 
      ccgactttg accgacgaa caagcggca aaaattgtc 
      gcattgaat agtcttgat atcgagttg tataaatac 
      gccaaagaa ttgttgttt aaacgattc gaacggtta 
      agggcgaga gatagcgat tttaataag aggtatagt 
      catttgggt gagttgact gcaaggagt ggtgtcacg 
      gagttcgat tgggatagg ttggttgaa gatgcgacc 
      gtttcgaat tag
    mRNA sequence
    • >TC014815 RA
      cagtttatt ttttgtctg tcaatatgt ctacaagtt 
      tggcaattc cacattcta agtgcgaat ggtagtgag 
      aggaggtct tctggtaac atgttgaaa acttaacag 
      ttgcgttcg gtgtaatga ataatgaag aatttgaag 
      agcgtgtcg gtgcaagta attgacagc gacatagcc 
      ttgctgttg ccgaatggt gtgtatgaa ataaaacaa 
      atggagcca aaatcttcc agattgaga cggataggg 
      attttctgt gtggctctg gccctattc ggcatcgta 
      ggtttcggc tacttttgt ccagacaaa gtatgtgcc 
      cttagtaat aaagaagca tccatatca ctagaagac 
      aatttaggg aaatcgctc tctcaagct agcagtaaa 
      gtaggttta agttatgag gatatgttg aacgatgat 
      tttttcgat atggattca catgatgta atggtgttt 
      ctccacatg cagaagact ggtggcacc tcctttggc 
      aaacacctc gtccacgac ctggacttg aaacgtcca 
      tgcacttgt ccactaacc cggaagcgt tgctattgt 
      ttccgtccg cattccaac caatcgtgg ctcttcagc 
      cgctacacc accggctgg aaatgcggc ctccatgcc 
      gactggacc gaactaacc agttgtgta gacgaaaaa 
      ctcgacgaa cttgaaggt aacgtcgtc aaaagacgc 
      tacttctac atcaccctt cttcgagag cccgttgct 
      cgatatctg agcgaattc agacatgtc caacgtggg 
      gccacctgg aagggctcc cgacacttc tgcagaggg 
      cgtccagcc accgacgac gaaataccc ccatgttac 
      gagggcccc gattggggc ggagtcgcc cttgatgag 
      ttcatcgaa tgcgagagt aatttggca gtgaatcgc 
      caaacgcga atgttggca gatctcgaa ttggtgggt 
      tgttatgat aagagttac atgtcgaag gcggataga 
      gacaggacg atgttgata agcgcgaaa aaaaatctc 
      gaatcgatg gcgtttttt ggtttaaca gaataccaa 
      aaaatgtcg cagtatatt ttcgaggag acttttaat 
      ttgaggttt gcggtgccg tttgagcaa aataatgcg 
      acggtttcg agttcaaca atgccgact ttgaccgac 
      gaacaagcg gcaaaaatt gtcgcattg aatagtctt 
      gatatcgag ttgtataaa tacgccaaa gaattgttg 
      tttaaacga ttcgaacgg ttaagggcg agagatagc 
      gattttaat aagaggtat agtcatttg ggtgagttg 
      actgcaagg agtggtgtc acggagttc gattgggat 
      aggttggtt gaagatgcg accgtttcg aattagagt 
      gccatatta tttgtgtat cacgcacgt gtttggatt 
      taaaatggt ttgtttgag gaaatgcgg tcatcagtg 
      acgataaaa tatgaccct gtgctgatt caactataa 
      ttggagagt ttttatgtt tcgactgtc ggtttagag 
      ataagatta aaaaattaa ataattaca agaaaaagt 
      acaaattaa ccactcgtc ttcggctcg tgtcgcaaa 
      ttgtggtgt tttttattg tcactgaat gaccgctcc 
      atcgacaat aaggtaaac gggagtagc aagaacaaa 
      ggaccgttt tctattcta aaattagcg ttcaaagcc 
      gtctccaaa acatcacac ttcatcaaa tttgaaaaa 
      tgcgagcca cacatgcaa caccacatt ttctattta 
      tcactagcg ttttttagt tttgttcat tttccattt 
      gtaataaca ctgccttga gttaagcgt agatcttat 
      taatattaa tatgcgtat accaaagac gaaaataat 
      attcttaga tgcagctaa cttattttt aggattatt 
      ttagtgcaa tttcaacca ttagttttt atttaatta 
      aattaatga cagggttaa gtaagtaat cgaactgat 
      gccgtaatt ttttaagga agattattg ttgttaaga 
      caatctggt gccctatta tagtgattg ttatatttt 
      acaagtctc ttgtgtcgt gcagttgtc aatactgtg 
      tattgcatt tattttaat gatatataa cgaacatat 
      cgtctccta attatgtta ttgactgat ttaatttgt 
      aatacttgt aggtacatt tgtagtact tagttgttt 
      ttaccacaa atttaaaca gacctttag tttaataat 
      acaactctt gtaacatat atttaggtg catatatca 
      tacgagtac gtacaaaaa aatatatac tttttacaa 
      gaattacaa acatttaat tcaccctcc caaacgaca 
      catttcaag tgatggaat acttttatt gaattcatt 
      acatgatat gtgaaacgt gttcttaaa tatttgaga 
      gaaagcgta actgtcttt ttaactata attattatt 
      attacgata ttctggaaa caaatgcat tggatggag 
      atttttttt tcacattaa gtatataca ttcaagact 
      tacacattt atacacatt tatcaatgt tattatttg 
      ttcgtaaac ctattaaaa taaaataaa taattaaaa 
      aaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY