Status
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Gene information
General information
TC014809 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15354238..15357115
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014809
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02335

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4
    egg chamber complete not present - Number of animals showing the phenotype: 1

    egg chamber number decreased - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014809 RA
      MSTVLNVFN VNIKLINRQ LAHCYNVRS FSYIHSKRK 
      SSKFLPVIY QSQRVYASK DVESNVSGG ILAKTLQRF 
      TALPKGQTV RECFHPGAS ALSRDALPI NTRQVVTGT 
      QMVTAMLQY IWPKDNKAV RDRVTLAVG LLIGAKVLN 
      VSVPFIFKY SIDYLNSAG TLNLETAPA TVATFATSL 
      LIAYGLARA GAAGFNELR NAVFAKVAQ HSIRRIAKN 
      VFLHLHNLD LGFHLSRQT GALSKTIDR GSRGINFVL 
      SAMVFNVVP TVFELALVS SILGLKCGA AFAGISLGC 
      VGIYAAYTL GITQWRTKF RVYMNQAEN EAGNKAIDS 
      LINYETVKY FNNEKFEAE RYDNVLKKY EAASLKTSS 
      SLALLNFGQ NAIFSAAIS GIMVLAANE IIKGNMTVG 
      DLVMVNGLL FQLSVPLGF LGSVYREVR QALIDMQTM 
      FTLMTMDPA IKSKTHAPY LHVDSKTSD IRFENVSFS 
      YGPGKDILK DLNFTIAPG KKVAIIGGS GSGKSTIVR 
      LLYRFYEPS SGRILIGNQ DIRDVDLES LRRAISIVP 
      QDSVLFHDT IKHNLHYGD LMAKEEDVI NAAKMAEIH 
      DSIIKWPQG YQTQVGERG LKLSGGEKQ RVAIARAIL 
      KNSPILVFD EATSSLDSL TEHNILTAL KRATQGRTS 
      ICIAHRLST VMDADEILV LENGQVSEV GTHESLIQN 
      RESLYYRLW RTQNHQDLG DNTRESFKN
    CDS sequence
    • >TC014809 RA
      atgtccacc gttttaaat gttttcaat gtcaatatt 
      aagttaata aatcgtcaa ttggcccat tgttacaat 
      gtcagaagc tttagttac atacattcc aaacgaaaa 
      tcgtcgaaa tttcttcca gtcatctac caaagtcag 
      cgggtttat gccagtaaa gacgttgag tcaaatgta 
      agcggtggc atcttggca aaaaccttg caacgtttt 
      acggcactt ccaaagggc caaacagtc cgagaatgt 
      tttcatccg ggagcttcg gccctgtct cgggacgcc 
      cttcctata aataccaga caggtcgta acagggacc 
      caaatggtc acagcaatg ttacaatac atttggccc 
      aaagataac aaagctgta agagatagg gtaacttta 
      gctgttgga cttctgatt ggtgccaaa gttttaaat 
      gtatcggtc cctttcatt tttaaatat tctatagat 
      tatctaaat tctgcaggc actttgaat ttggaaaca 
      gcacctgcc actgttgct acattcgcc acaagtttg 
      cttattgct tacggtctt gctcgagca ggcgctgcc 
      ggttttaac gaactgaga aatgccgtt tttgctaag 
      gttgcgcaa cattcaatt aggagaatt gcaaagaat 
      gttttttta catttgcac aacttggac ctcggtttc 
      catttaagt cgacaaacc ggtgcccta tctaaaaca 
      atagatcga gggtcgcgc ggtatcaat tttgtttta 
      tcagctatg gtttttaat gttgttcca actgttttt 
      gaattggct ctggtttcg agtattttg gggttgaaa 
      tgtggggct gctttcgca ggaatttca ttgggatgt 
      gttggtatt tatgccgct tatacgctg ggaattaca 
      caatggcgt accaagttt agagtgtac atgaatcag 
      gccgaaaat gaggcggga aataaagca attgattcg 
      ctcatcaat tacgaaacg gttaaatac tttaacaac 
      gagaagttc gaagctgaa cgttacgac aacgttttg 
      aaaaaatac gaagctgcc agtttaaaa accagttca 
      agcttggca ttgttaaat tttggccaa aacgcaatt 
      ttcagtgct gccattagt ggtatcatg gttttggca 
      gcaaatgaa ataatcaag ggaaatatg acagttggt 
      gatctagtg atggtaaac ggactatta ttccaactc 
      tcagtccct ttaggtttt ttgggtagt gtttatcgc 
      gaagtgaga caagcacta attgatatg caaacaatg 
      ttcactctc atgacaatg gatccagct attaaaagt 
      aaaacacac gcgccctat ttacacgtc gattccaaa 
      acatcggac atccgattc gaaaacgtg tctttttcg 
      tacggacca ggaaaagac attttgaaa gatttaaat 
      tttacaatt gctccgggt aaaaaagtt gccattatt 
      ggtggatca ggctcagga aaatcaaca atagtccgc 
      cttttgtat cgcttttac gaaccttcc tctggtcgt 
      atcttaatc ggcaatcaa gacattcgt gatgtggat 
      ttggaaagt ttaagacgt gcaatttca attgtccct 
      caagattct gttttgttt cacgataca atcaaacac 
      aacttgcac tacggtgat ttgatggcg aaagaggaa 
      gatgtaata aatgccgca aaaatggcc gaaattcac 
      gactcgata ataaagtgg ccccaaggc taccaaaca 
      caagtcggc gaaaggggg ctgaagctc tcagggggc 
      gagaaacaa cgggttgct atcgctcga gctattttg 
      aaaaattca ccaattctg gtctttgac gaagcgacg 
      agttcgcta gattctctc accgaacat aatatttta 
      actgctttg aagcgagcg acacaagga cggacaagt 
      atttgcata gcacacaga ttgagtacc gtaatggat 
      gctgacgag attttggtt cttgaaaat gggcaagtt 
      tctgaagtt ggtactcac gaaagttta atacaaaat 
      cgggaaagt ttgtattat cgcttgtgg aggacgcaa 
      aaccatcaa gatttgggc gataatacg agagagtcg 
      tttaaaaat tag
    mRNA sequence
    • >TC014809 RA
      tgctttttg acacttgtc taaaatgtc caccgtttt 
      aaatgtttt caatgtcaa tattaagtt aataaatcg 
      tcaattggc ccattgtta caatgtcag aagctttag 
      ttacataca ttccaaacg aaaatcgtc gaaatttct 
      tccagtcat ctaccaaag tcagcgggt ttatgccag 
      taaagacgt tgagtcaaa tgtaagcgg tggcatctt 
      ggcaaaaac cttgcaacg ttttacggc acttccaaa 
      gggccaaac agtccgaga atgttttca tccgggagc 
      ttcggccct gtctcggga cgcccttcc tataaatac 
      cagacaggt cgtaacagg gacccaaat ggtcacagc 
      aatgttaca atacatttg gcccaaaga taacaaagc 
      tgtaagaga tagggtaac tttagctgt tggacttct 
      gattggtgc caaagtttt aaatgtatc ggtcccttt 
      catttttaa atattctat agattatct aaattctgc 
      aggcacttt gaatttgga aacagcacc tgccactgt 
      tgctacatt cgccacaag tttgcttat tgcttacgg 
      tcttgctcg agcaggcgc tgccggttt taacgaact 
      gagaaatgc cgtttttgc taaggttgc gcaacattc 
      aattaggag aattgcaaa gaatgtttt tttacattt 
      gcacaactt ggacctcgg tttccattt aagtcgaca 
      aaccggtgc cctatctaa aacaataga tcgagggtc 
      gcgcggtat caattttgt tttatcagc tatggtttt 
      taatgttgt tccaactgt ttttgaatt ggctctggt 
      ttcgagtat tttggggtt gaaatgtgg ggctgcttt 
      cgcaggaat ttcattggg atgtgttgg tatttatgc 
      cgcttatac gctgggaat tacacaatg gcgtaccaa 
      gtttagagt gtacatgaa tcaggccga aaatgaggc 
      gggaaataa agcaattga ttcgctcat caattacga 
      aacggttaa atactttaa caacgagaa gttcgaagc 
      tgaacgtta cgacaacgt tttgaaaaa atacgaagc 
      tgccagttt aaaaaccag ttcaagctt ggcattgtt 
      aaattttgg ccaaaacgc aattttcag tgctgccat 
      tagtggtat catggtttt ggcagcaaa tgaaataat 
      caagggaaa tatgacagt tggtgatct agtgatggt 
      aaacggact attattcca actctcagt ccctttagg 
      ttttttggg tagtgttta tcgcgaagt gagacaagc 
      actaattga tatgcaaac aatgttcac tctcatgac 
      aatggatcc agctattaa aagtaaaac acacgcgcc 
      ctatttaca cgtcgattc caaaacatc ggacatccg 
      attcgaaaa cgtgtcttt ttcgtacgg accaggaaa 
      agacatttt gaaagattt aaattttac aattgctcc 
      gggtaaaaa agttgccat tattggtgg atcaggctc 
      aggaaaatc aacaatagt ccgcctttt gtatcgctt 
      ttacgaacc ttcctctgg tcgtatctt aatcggcaa 
      tcaagacat tcgtgatgt ggatttgga aagtttaag 
      acgtgcaat ttcaattgt ccctcaaga ttctgtttt 
      gtttcacga tacaatcaa acacaactt gcactacgg 
      tgatttgat ggcgaaaga ggaagatgt aataaatgc 
      cgcaaaaat ggccgaaat tcacgactc gataataaa 
      gtggcccca aggctacca aacacaagt cggcgaaag 
      ggggctgaa gctctcagg gggcgagaa acaacgggt 
      tgctatcgc tcgagctat tttgaaaaa ttcaccaat 
      tctggtctt tgacgaagc gacgagttc gctagattc 
      tctcaccga acataatat tttaactgc tttgaagcg 
      agcgacaca aggacggac aagtatttg catagcaca 
      cagattgag taccgtaat ggatgctga cgagatttt 
      ggttcttga aaatgggca agtttctga agttggtac 
      tcacgaaag tttaataca aaatcggga aagtttgta 
      ttatcgctt gtggaggac gcaaaacca tcaagattt 
      gggcgataa tacgagaga gtcgtttaa aaattagaa 
      acaggagtt tttgttgtt aattttatt aatgtaaat 
      actgtatat acgtatacg aagtgattt aatttaaat 
      aaaactaaa tgtggaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY