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Gene information
General information
TC014798 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 7301064..7305107
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014798
    iBeetle screen iB_02334
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 83.33% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 9

    wings not closed - Number of animals showing the phenotype: 9

    female genital lobes decreased - Number of animals showing the phenotype: 9

    Adult morphology
    antenna shortened - Number of animals showing the phenotype: 5


    legs tarsus potentially fused - Number of animals showing the phenotype: 5


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014798 RA
      MASSNSGVQ QHYQPQLNT SPHHCKECG LYLNSAESL 
      EVHLQYHKE NLLNKWATQ AATSHSEET NNNNPKAIK 
      REFIPNNTI AAADSSDSM NKKSPDYSR TTPETIFGH 
      PPTPQSYQS ASSPYQNHD NSAFSPNFQ NFQLKQERT 
      SSPNYPNNY PNFAESQFF PMDNQNQQY SQEFIHKAP 
      SYRYHPYQQ YDRSQSNAQ VSSSSPAYP PQPTPSPSP 
      KQCDKCGYV CDSAAQLIE HQNLAHPPT PAYHPNHFL 
      FNNDHQPQI KSEEDAQSE ILDLDSHKV HQVYPEDEK 
      RQNGDMNGP NPHSVTALL NTWSPPQQQ QQQKMYQQQ 
      QQYMNGPQV MHNQEFMGG NTVTTTSPQ DQGYRPFEH 
      LPAQPSAPV ITSTQVPTN PPQTAVSPV TKSANWKSN 
      EARRPKTYN CTACNKWFT SSGHLKRHY NTTLHKNAV 
      KSSGQPDPA SMPISAHHH PARDSASNK EDANNSPGD 
      EDTNSMPMP APYDRPPNG EAGPSPTSL DSRGLLHGF 
      NMGPPQPHM MPLDSPQFQ MYPNGSAPH VTQPTATSN 
      ISITGEFDD LQQDSQPLP SFAQFHRYG LLTSYGNAN 
      VGGGGPATA PYCYYPDPF ESAETRTLM YKTDDDEYR 
      LTVLTVADP APPEDMAPQ HETDSLALS APPSPEKAH 
      IKRDYEEST GSPQITSTV QVAKPGLYK CIDCDKTFN 
      KACYLTQHN KTFHCGDKP FKCTRCGKR FSNEETHEE 
      HASKHAGDK PHKCDRCPK QFNHKTDLR RHMCLHTGQ 
      KPFVCDTCG KGFIRKDHM LKHCETHFR KTQNKNRG
    CDS sequence
    • >TC014798 RA
      atggcaagc agcaacagt ggcgtgcaa caacactac 
      cagccgcag ctcaacacg agccctcat cattgcaag 
      gaatgcggc ctgtatctc aactcggcc gagtccctt 
      gaagtccac ctccagtac cacaaagag aacctcctc 
      aacaaatgg gcgacccaa gcggccact tcccactcg 
      gaagaaacc aacaataat aatcccaaa gccatcaaa 
      cgtgagttt ataccaaat aatactatt gccgcggcc 
      gatagttca gactccatg aacaagaaa agtcccgac 
      tacagtcga acaaccccc gagacgatt ttcggacat 
      ccaccgacg cctcaaagt tatcaaagc gcatcttct 
      ccctatcag aaccacgac aattccgct ttttcgcca 
      aactttcaa aactttcaa ttgaaacaa gaacgtact 
      tcttcgccc aactatccc aataattac cccaatttc 
      gccgaatcg caattcttt ccaatggac aaccagaac 
      caacaatac tcgcaagaa ttcatacat aaagcccct 
      tcgtacaga taccacccc tatcaacag tacgaccgg 
      tctcaatct aacgcacag gtgtcttcc tcgagtccg 
      gcatacccg ccccagccg acaccttcc cctagcccc 
      aaacaatgc gacaagtgc ggctacgtc tgcgattca 
      gccgcccaa ctcatcgaa caccaaaac ttggcccat 
      ccaccgact cctgcctat caccccaat cactttctt 
      ttcaataac gaccaccag ccccaaatc aagagcgaa 
      gaggatgcc caaagcgag attctagac ctggactcg 
      cataaagtc caccaggtg tatcccgaa gatgagaaa 
      cgacaaaac ggcgatatg aacggacca aacccacat 
      tccgtcacg gctttgctc aatacttgg tcgccccct 
      caacagcaa caacaacaa aagatgtac cagcagcag 
      cagcaatac atgaacggg ccccaagtc atgcacaac 
      caggagttc atgggtggg aataccgtg acgacgacg 
      tcgccacaa gaccagggc taccggccg tttgagcac 
      cttccagct cagcccagc gctccggtt ataacaagc 
      actcaagtt cctacgaat ccgccccag acggcggtc 
      tcgccggtc acaaagagc gccaactgg aagtcgaac 
      gaagcccgc cgccccaag acttacaat tgcaccgct 
      tgcaacaag tggttcact agctcgggc cacctcaaa 
      cgccactac aacaccacc ttgcacaaa aacgcggtc 
      aagtccagc gggcagccc gaccccgct tccatgccc 
      atcagcgcc caccaccac ccagcgcgc gactccgcc 
      tccaacaag gaggacgcc aacaacagc cccggagac 
      gaggacacc aacagcatg cccatgccg gcgccctac 
      gacaggccc ccaaacggg gaagcaggc ccctcaccc 
      accagcctc gactcgagg ggcctgctg catggtttc 
      aacatgggg ccaccccaa ccccatatg atgcccctc 
      gacagcccc cagttccag atgtaccca aacgggtcg 
      gccccccac gttacgcag cctacggct accagcaac 
      atcagtatt actggtgaa ttcgacgac ctccagcag 
      gactcgcag ccgctgccc agcttcgcg cagttccat 
      cgctacggc ctgctgacg agctacggt aacgcgaac 
      gtggggggc gggggtccg gcaacggcc ccttactgc 
      tactacccc gaccccttc gagagcgcc gagacgcgc 
      accctcatg tacaagacc gacgacgac gagtaccgc 
      ttgactgtg ctcacggtg gccgatccg gcgccccct 
      gaagacatg gccccgcag cacgagacg gattccttg 
      gcgctgagc gcgccgccg tcgcctgag aaagcgcac 
      atcaagcgc gactacgag gagagcacc ggcagcccc 
      cagataacc tccacggta caggtggcc aaaccgggc 
      ttgtacaag tgcatcgac tgcgacaag accttcaac 
      aaggcgtgc tatttgacg cagcacaac aagaccttc 
      cattgcgga gacaagccg ttcaaatgc acgcgctgc 
      ggaaagcgc ttctccaac gaggagact cacgaagag 
      cacgcctcc aagcatgcc ggcgacaag ccacacaag 
      tgtgaccga tgtcccaag caattcaac cacaagacg 
      gacctaagg cggcacatg tgcctgcac acgggtcag 
      aaacccttc gtctgcgac acctgtggt aaggggttc 
      atccggaaa gaccacatg ctgaagcac tgcgagacg 
      cacttcagg aaaacgcag aacaagaac cgagggtga
    mRNA sequence
    • >TC014798 RA
      ctaccaccc caaaacgca ttaaaccgg gcgaagcca 
      ttggtcgaa atcggagcg gtgcggcct gtgcgagtt 
      tgcgaaaca cgcatagca cgttggacc tccgaattc 
      gagaggaac cgtgaagcc ttcagtcgg tgttttgtc 
      cggcaagag tctagtgcg gtgtgccat catcatcaa 
      agtgttgac gaccagtgc gctcataaa caaccctcc 
      ggcttcagg atggttctg caagagtca catttcgtg 
      gtcgctgct cctccgaca taacatggg ctaaacgtc 
      cgcaaagac tgtttttat ttttttgtt gcgacttta 
      tttttgttt gccgaaagt gatgcaaac tgtaaacaa 
      gcgcggttt attttctgt ttcgaatgt gatgcctcc 
      gggaataag aactattgc gatgttatg atattaaaa 
      gagtgacag ttgtatttt tttatttgg aacactttg 
      ttttgttgt ttgagaaac tttaacgcg agtgcaatg 
      gcaagcagc aacagtggc gtgcaacaa cactaccag 
      ccgcagctc aacacgagc cctcatcat tgcaaggaa 
      tgcggcctg tatctcaac tcggccgag tcccttgaa 
      gtccacctc cagtaccac aaagagaac ctcctcaac 
      aaatgggcg acccaagcg gccacttcc cactcggaa 
      gaaaccaac aataataat cccaaagcc atcaaacgt 
      gagtttata ccaaataat actattgcc gcggccgat 
      agttcagac tccatgaac aagaaaagt cccgactac 
      agtcgaaca acccccgag acgattttc ggacatcca 
      ccgacgcct caaagttat caaagcgca tcttctccc 
      tatcagaac cacgacaat tccgctttt tcgccaaac 
      tttcaaaac tttcaattg aaacaagaa cgtacttct 
      tcgcccaac tatcccaat aattacccc aatttcgcc 
      gaatcgcaa ttctttcca atggacaac cagaaccaa 
      caatactcg caagaattc atacataaa gccccttcg 
      tacagatac cacccctat caacagtac gaccggtct 
      caatctaac gcacaggtg tcttcctcg agtccggca 
      tacccgccc cagccgaca ccttcccct agccccaaa 
      caatgcgac aagtgcggc tacgtctgc gattcagcc 
      gcccaactc atcgaacac caaaacttg gcccatcca 
      ccgactcct gcctatcac cccaatcac tttcttttc 
      aataacgac caccagccc caaatcaag agcgaagag 
      gatgcccaa agcgagatt ctagacctg gactcgcat 
      aaagtccac caggtgtat cccgaagat gagaaacga 
      caaaacggc gatatgaac ggaccaaac ccacattcc 
      gtcacggct ttgctcaat acttggtcg ccccctcaa 
      cagcaacaa caacaaaag atgtaccag cagcagcag 
      caatacatg aacgggccc caagtcatg cacaaccag 
      gagttcatg ggtgggaat accgtgacg acgacgtcg 
      ccacaagac cagggctac cggccgttt gagcacctt 
      ccagctcag cccagcgct ccggttata acaagcact 
      caagttcct acgaatccg ccccagacg gcggtctcg 
      ccggtcaca aagagcgcc aactggaag tcgaacgaa 
      gcccgccgc cccaagact tacaattgc accgcttgc 
      aacaagtgg ttcactagc tcgggccac ctcaaacgc 
      cactacaac accaccttg cacaaaaac gcggtcaag 
      tccagcggg cagcccgac cccgcttcc atgcccatc 
      agcgcccac caccaccca gcgcgcgac tccgcctcc 
      aacaaggag gacgccaac aacagcccc ggagacgag 
      gacaccaac agcatgccc atgccggcg ccctacgac 
      aggccccca aacggggaa gcaggcccc tcacccacc 
      agcctcgac tcgaggggc ctgctgcat ggtttcaac 
      atggggcca ccccaaccc catatgatg cccctcgac 
      agcccccag ttccagatg tacccaaac gggtcggcc 
      ccccacgtt acgcagcct acggctacc agcaacatc 
      agtattact ggtgaattc gacgacctc cagcaggac 
      tcgcagccg ctgcccagc ttcgcgcag ttccatcgc 
      tacggcctg ctgacgagc tacggtaac gcgaacgtg 
      gggggcggg ggtccggca acggcccct tactgctac 
      taccccgac cccttcgag agcgccgag acgcgcacc 
      ctcatgtac aagaccgac gacgacgag taccgcttg 
      actgtgctc acggtggcc gatccggcg ccccctgaa 
      gacatggcc ccgcagcac gagacggat tccttggcg 
      ctgagcgcg ccgccgtcg cctgagaaa gcgcacatc 
      aagcgcgac tacgaggag agcaccggc agcccccag 
      ataacctcc acggtacag gtggccaaa ccgggcttg 
      tacaagtgc atcgactgc gacaagacc ttcaacaag 
      gcgtgctat ttgacgcag cacaacaag accttccat 
      tgcggagac aagccgttc aaatgcacg cgctgcgga 
      aagcgcttc tccaacgag gagactcac gaagagcac 
      gcctccaag catgccggc gacaagcca cacaagtgt 
      gaccgatgt cccaagcaa ttcaaccac aagacggac 
      ctaaggcgg cacatgtgc ctgcacacg ggtcagaaa 
      cccttcgtc tgcgacacc tgtggtaag gggttcatc 
      cggaaagac cacatgctg aagcactgc gagacgcac 
      ttcaggaaa acgcagaac aagaaccga gggtgagcg 
      ttgcgacac tccaaagac cgactgcac taggtgtgt 
      acgtagttt ttatttttg tcacttggc acattccta 
      cttaaatgt tgtaactat ttgaatgaa tattttatt 
      atcttgtac atatattaa ttttaatgt ttaagtttc 
      gaaagatat tttttaaaa ctatttgaa gacgatcaa 
      atgattcaa atggacaac agagtaata aacaagaaa 
      cacagagta attgctcaa gtatctttt ccgcaagtc 
      cgataataa acaatattc aaaacagca atacctcag 
      aatctcagt taaaataga tataaatat ttatatcag 
      attatattg atatctcgt ggatgtaca aactttagg 
      atcaagttt attaaagat gtataaaat aaaaagtac 
      agatcatta tagtgccgg ctgtcggcg gttcgttcc 
      ccggagtcc cggacgcgt gatccgggc accgatccg 
      cgctccggt tcatagtaa attcgaagt aataatatg 
      caataatgt tacaacgca agttttgat atttgtttt 
      ttaagatgt aataagagt tgtgagtcg ttgtgtaca 
      tacatattt acgttgtta ggtaatata ttttttcca 
      tgacgaatc tacctcagt taaagctga tgaatttgt 
      gtgccaatt attctttag ttttccagc ccttgcaag 
      ggtcatgtg ttaatgaac gattttatc tcgtgccaa 
      attttatac agacagcag caaaatctt gctatattt 
      ttaatatat gacaacatt ttgctaccg gtggcatgc 
      gtatgttac tactattag tgcaatttg tttttttaa 
      ttagttttt ttgacatga agcgccact ttcagttag 
      ggacagtta ccaaataat gttcgtagg tcgattgat 
      tgtttctgt tacggggag ttatgtgga tgtgatttg 
      ctgttatgt ttacgacta ctggcgata cttactggc 
      gcaatttta cgcatatag tcgggtatg cagttattg 
      tgtattatt tgttaagac tgattgtat gtagtaatt 
      atgttccat aggttgcta ttgtatatt tttcctgat 
      atgttacag ctgttagtc ttaagtttt taaataaat 
      gttattatg ttactttac caatgacac aaataaata 
      taaatcatt att