Status
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Gene information
General information
TC014798 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15216478..15220521
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014798
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02334

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 83.33% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 9

    wings not closed - Number of animals showing the phenotype: 9

    female genital lobes decreased - Number of animals showing the phenotype: 9

    Adult morphology
    antenna shortened - Number of animals showing the phenotype: 5


    legs tarsus potentially fused - Number of animals showing the phenotype: 5


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014798 RA
      MASSNSGVQ QHYQPQLNT SPHHCKECG LYLNSAESL 
EVHLQYHKE NLLNKWATQ AATSHSEET NNNNPKAIK 
REFIPNNTI AAADSSDSM NKKSPDYSR TTPETIFGH 
PPTPQSYQS ASSPYQNHD NSAFSPNFQ NFQLKQERT 
SSPNYPNNY PNFAESQFF PMDNQNQQY SQEFIHKAP 
SYRYHPYQQ YDRSQSNAQ VSSSSPAYP PQPTPSPSP 
KQCDKCGYV CDSAAQLIE HQNLAHPPT PAYHPNHFL 
FNNDHQPQI KSEEDAQSE ILDLDSHKV HQVYPEDEK 
RQNGDMNGP NPHSVTALL NTWSPPQQQ QQQKMYQQQ 
QQYMNGPQV MHNQEFMGG NTVTTTSPQ DQGYRPFEH 
LPAQPSAPV ITSTQVPTN PPQTAVSPV TKSANWKSN 
EARRPKTYN CTACNKWFT SSGHLKRHY NTTLHKNAV 
KSSGQPDPA SMPISAHHH PARDSASNK EDANNSPGD 
EDTNSMPMP APYDRPPNG EAGPSPTSL DSRGLLHGF 
NMGPPQPHM MPLDSPQFQ MYPNGSAPH VTQPTATSN 
ISITGEFDD LQQDSQPLP SFAQFHRYG LLTSYGNAN 
VGGGGPATA PYCYYPDPF ESAETRTLM YKTDDDEYR 
LTVLTVADP APPEDMAPQ HETDSLALS APPSPEKAH 
IKRDYEEST GSPQITSTV QVAKPGLYK CIDCDKTFN 
KACYLTQHN KTFHCGDKP FKCTRCGKR FSNEETHEE 
HASKHAGDK PHKCDRCPK QFNHKTDLR RHMCLHTGQ 
KPFVCDTCG KGFIRKDHM LKHCETHFR KTQNKNRG
    CDS sequence
    • >TC014798 RA
      atggcaagc agcaacagt ggcgtgcaa caacactac 
cagccgcag ctcaacacg agccctcat cattgcaag 
gaatgcggc ctgtatctc aactcggcc gagtccctt 
gaagtccac ctccagtac cacaaagag aacctcctc 
aacaaatgg gcgacccaa gcggccact tcccactcg 
gaagaaacc aacaataat aatcccaaa gccatcaaa 
cgtgagttt ataccaaat aatactatt gccgcggcc 
gatagttca gactccatg aacaagaaa agtcccgac 
tacagtcga acaaccccc gagacgatt ttcggacat 
ccaccgacg cctcaaagt tatcaaagc gcatcttct 
ccctatcag aaccacgac aattccgct ttttcgcca 
aactttcaa aactttcaa ttgaaacaa gaacgtact 
tcttcgccc aactatccc aataattac cccaatttc 
gccgaatcg caattcttt ccaatggac aaccagaac 
caacaatac tcgcaagaa ttcatacat aaagcccct 
tcgtacaga taccacccc tatcaacag tacgaccgg 
tctcaatct aacgcacag gtgtcttcc tcgagtccg 
gcatacccg ccccagccg acaccttcc cctagcccc 
aaacaatgc gacaagtgc ggctacgtc tgcgattca 
gccgcccaa ctcatcgaa caccaaaac ttggcccat 
ccaccgact cctgcctat caccccaat cactttctt 
ttcaataac gaccaccag ccccaaatc aagagcgaa 
gaggatgcc caaagcgag attctagac ctggactcg 
cataaagtc caccaggtg tatcccgaa gatgagaaa 
cgacaaaac ggcgatatg aacggacca aacccacat 
tccgtcacg gctttgctc aatacttgg tcgccccct 
caacagcaa caacaacaa aagatgtac cagcagcag 
cagcaatac atgaacggg ccccaagtc atgcacaac 
caggagttc atgggtggg aataccgtg acgacgacg 
tcgccacaa gaccagggc taccggccg tttgagcac 
cttccagct cagcccagc gctccggtt ataacaagc 
actcaagtt cctacgaat ccgccccag acggcggtc 
tcgccggtc acaaagagc gccaactgg aagtcgaac 
gaagcccgc cgccccaag acttacaat tgcaccgct 
tgcaacaag tggttcact agctcgggc cacctcaaa 
cgccactac aacaccacc ttgcacaaa aacgcggtc 
aagtccagc gggcagccc gaccccgct tccatgccc 
atcagcgcc caccaccac ccagcgcgc gactccgcc 
tccaacaag gaggacgcc aacaacagc cccggagac 
gaggacacc aacagcatg cccatgccg gcgccctac 
gacaggccc ccaaacggg gaagcaggc ccctcaccc 
accagcctc gactcgagg ggcctgctg catggtttc 
aacatgggg ccaccccaa ccccatatg atgcccctc 
gacagcccc cagttccag atgtaccca aacgggtcg 
gccccccac gttacgcag cctacggct accagcaac 
atcagtatt actggtgaa ttcgacgac ctccagcag 
gactcgcag ccgctgccc agcttcgcg cagttccat 
cgctacggc ctgctgacg agctacggt aacgcgaac 
gtggggggc gggggtccg gcaacggcc ccttactgc 
tactacccc gaccccttc gagagcgcc gagacgcgc 
accctcatg tacaagacc gacgacgac gagtaccgc 
ttgactgtg ctcacggtg gccgatccg gcgccccct 
gaagacatg gccccgcag cacgagacg gattccttg 
gcgctgagc gcgccgccg tcgcctgag aaagcgcac 
atcaagcgc gactacgag gagagcacc ggcagcccc 
cagataacc tccacggta caggtggcc aaaccgggc 
ttgtacaag tgcatcgac tgcgacaag accttcaac 
aaggcgtgc tatttgacg cagcacaac aagaccttc 
cattgcgga gacaagccg ttcaaatgc acgcgctgc 
ggaaagcgc ttctccaac gaggagact cacgaagag 
cacgcctcc aagcatgcc ggcgacaag ccacacaag 
tgtgaccga tgtcccaag caattcaac cacaagacg 
gacctaagg cggcacatg tgcctgcac acgggtcag 
aaacccttc gtctgcgac acctgtggt aaggggttc 
atccggaaa gaccacatg ctgaagcac tgcgagacg 
cacttcagg aaaacgcag aacaagaac cgagggtga
    mRNA sequence
    • >TC014798 RA
      ctaccaccc caaaacgca ttaaaccgg gcgaagcca 
ttggtcgaa atcggagcg gtgcggcct gtgcgagtt 
tgcgaaaca cgcatagca cgttggacc tccgaattc 
gagaggaac cgtgaagcc ttcagtcgg tgttttgtc 
cggcaagag tctagtgcg gtgtgccat catcatcaa 
agtgttgac gaccagtgc gctcataaa caaccctcc 
ggcttcagg atggttctg caagagtca catttcgtg 
gtcgctgct cctccgaca taacatggg ctaaacgtc 
cgcaaagac tgtttttat ttttttgtt gcgacttta 
tttttgttt gccgaaagt gatgcaaac tgtaaacaa 
gcgcggttt attttctgt ttcgaatgt gatgcctcc 
gggaataag aactattgc gatgttatg atattaaaa 
gagtgacag ttgtatttt tttatttgg aacactttg 
ttttgttgt ttgagaaac tttaacgcg agtgcaatg 
gcaagcagc aacagtggc gtgcaacaa cactaccag 
ccgcagctc aacacgagc cctcatcat tgcaaggaa 
tgcggcctg tatctcaac tcggccgag tcccttgaa 
gtccacctc cagtaccac aaagagaac ctcctcaac 
aaatgggcg acccaagcg gccacttcc cactcggaa 
gaaaccaac aataataat cccaaagcc atcaaacgt 
gagtttata ccaaataat actattgcc gcggccgat 
agttcagac tccatgaac aagaaaagt cccgactac 
agtcgaaca acccccgag acgattttc ggacatcca 
ccgacgcct caaagttat caaagcgca tcttctccc 
tatcagaac cacgacaat tccgctttt tcgccaaac 
tttcaaaac tttcaattg aaacaagaa cgtacttct 
tcgcccaac tatcccaat aattacccc aatttcgcc 
gaatcgcaa ttctttcca atggacaac cagaaccaa 
caatactcg caagaattc atacataaa gccccttcg 
tacagatac cacccctat caacagtac gaccggtct 
caatctaac gcacaggtg tcttcctcg agtccggca 
tacccgccc cagccgaca ccttcccct agccccaaa 
caatgcgac aagtgcggc tacgtctgc gattcagcc 
gcccaactc atcgaacac caaaacttg gcccatcca 
ccgactcct gcctatcac cccaatcac tttcttttc 
aataacgac caccagccc caaatcaag agcgaagag 
gatgcccaa agcgagatt ctagacctg gactcgcat 
aaagtccac caggtgtat cccgaagat gagaaacga 
caaaacggc gatatgaac ggaccaaac ccacattcc 
gtcacggct ttgctcaat acttggtcg ccccctcaa 
cagcaacaa caacaaaag atgtaccag cagcagcag 
caatacatg aacgggccc caagtcatg cacaaccag 
gagttcatg ggtgggaat accgtgacg acgacgtcg 
ccacaagac cagggctac cggccgttt gagcacctt 
ccagctcag cccagcgct ccggttata acaagcact 
caagttcct acgaatccg ccccagacg gcggtctcg 
ccggtcaca aagagcgcc aactggaag tcgaacgaa 
gcccgccgc cccaagact tacaattgc accgcttgc 
aacaagtgg ttcactagc tcgggccac ctcaaacgc 
cactacaac accaccttg cacaaaaac gcggtcaag 
tccagcggg cagcccgac cccgcttcc atgcccatc 
agcgcccac caccaccca gcgcgcgac tccgcctcc 
aacaaggag gacgccaac aacagcccc ggagacgag 
gacaccaac agcatgccc atgccggcg ccctacgac 
aggccccca aacggggaa gcaggcccc tcacccacc 
agcctcgac tcgaggggc ctgctgcat ggtttcaac 
atggggcca ccccaaccc catatgatg cccctcgac 
agcccccag ttccagatg tacccaaac gggtcggcc 
ccccacgtt acgcagcct acggctacc agcaacatc 
agtattact ggtgaattc gacgacctc cagcaggac 
tcgcagccg ctgcccagc ttcgcgcag ttccatcgc 
tacggcctg ctgacgagc tacggtaac gcgaacgtg 
gggggcggg ggtccggca acggcccct tactgctac 
taccccgac cccttcgag agcgccgag acgcgcacc 
ctcatgtac aagaccgac gacgacgag taccgcttg 
actgtgctc acggtggcc gatccggcg ccccctgaa 
gacatggcc ccgcagcac gagacggat tccttggcg 
ctgagcgcg ccgccgtcg cctgagaaa gcgcacatc 
aagcgcgac tacgaggag agcaccggc agcccccag 
ataacctcc acggtacag gtggccaaa ccgggcttg 
tacaagtgc atcgactgc gacaagacc ttcaacaag 
gcgtgctat ttgacgcag cacaacaag accttccat 
tgcggagac aagccgttc aaatgcacg cgctgcgga 
aagcgcttc tccaacgag gagactcac gaagagcac 
gcctccaag catgccggc gacaagcca cacaagtgt 
gaccgatgt cccaagcaa ttcaaccac aagacggac 
ctaaggcgg cacatgtgc ctgcacacg ggtcagaaa 
cccttcgtc tgcgacacc tgtggtaag gggttcatc 
cggaaagac cacatgctg aagcactgc gagacgcac 
ttcaggaaa acgcagaac aagaaccga gggtgagcg 
ttgcgacac tccaaagac cgactgcac taggtgtgt 
acgtagttt ttatttttg tcacttggc acattccta 
cttaaatgt tgtaactat ttgaatgaa tattttatt 
atcttgtac atatattaa ttttaatgt ttaagtttc 
gaaagatat tttttaaaa ctatttgaa gacgatcaa 
atgattcaa atggacaac agagtaata aacaagaaa 
cacagagta attgctcaa gtatctttt ccgcaagtc 
cgataataa acaatattc aaaacagca atacctcag 
aatctcagt taaaataga tataaatat ttatatcag 
attatattg atatctcgt ggatgtaca aactttagg 
atcaagttt attaaagat gtataaaat aaaaagtac 
agatcatta tagtgccgg ctgtcggcg gttcgttcc 
ccggagtcc cggacgcgt gatccgggc accgatccg 
cgctccggt tcatagtaa attcgaagt aataatatg 
caataatgt tacaacgca agttttgat atttgtttt 
ttaagatgt aataagagt tgtgagtcg ttgtgtaca 
tacatattt acgttgtta ggtaatata ttttttcca 
tgacgaatc tacctcagt taaagctga tgaatttgt 
gtgccaatt attctttag ttttccagc ccttgcaag 
ggtcatgtg ttaatgaac gattttatc tcgtgccaa 
attttatac agacagcag caaaatctt gctatattt 
ttaatatat gacaacatt ttgctaccg gtggcatgc 
gtatgttac tactattag tgcaatttg tttttttaa 
ttagttttt ttgacatga agcgccact ttcagttag 
ggacagtta ccaaataat gttcgtagg tcgattgat 
tgtttctgt tacggggag ttatgtgga tgtgatttg 
ctgttatgt ttacgacta ctggcgata cttactggc 
gcaatttta cgcatatag tcgggtatg cagttattg 
tgtattatt tgttaagac tgattgtat gtagtaatt 
atgttccat aggttgcta ttgtatatt tttcctgat 
atgttacag ctgttagtc ttaagtttt taaataaat 
gttattatg ttactttac caatgacac aaataaata 
taaatcatt att

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY