Status
Please wait ...
Gene information
General information
TC031161
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014782,TC014781
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 7108302..7117918
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031161
    iBeetle screen iB_02332
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031161 RA
      MEFVKHTLL FCCFALWEI EAVLNKRNG GVQSFVQNY 
      NGHFDTTIH KVSFTQKGY IQFLRYITH VPPLSDYTF 
      CIWVRSHNL TYAHPLLSY SKHEEERLI RVWISPHGT 
      DINLEILQH PIFKIHTHF VENHWYHVC QSWSSSHAS 
      WSLYLNGKL KSSGFAPQL RGSKIKGGG DIVVGQEYT 
      DFDKGLDDG IEGDVFGFN FVLSPTSHS SHGDFPSRG 
      HYSTEIPSV LSYFSVEPN RQPKLFDFF ESMWSFFNP 
      THAIKVVPK KRGHFGARV ANDIEEYSP KPTGLKLVE 
      LSYHCALGK GAPVSGKDV LISWTKTPV RVFGGAILK 
      NIKPFCTDA KGNRRRVDI IDCKHYLAR SFTPTAVDM 
      TGSTNRIDF VNFICTCSM DACFAAFDK DCDGKLNLE 
      EFWALCRAL FRNSKGVTY EIERHTLET IFNVFDKNQ 
      DGSIDRDEF VTCWNLWIK TIVKPVSAA LIIDVQNDF 
      ITGSLNITN CPAKHHGGD VIQPINNLL ETVEFETVV 
      YSYDWHPND HVSFIDNIS LRKLHESSP ISAENAKVY 
      DTVVFDREP PMVQKLWPR HCVQNTWGS DLHKDLKVV 
      ENSTKIYKG TNPDVDSYS LFWDNQKLT DTKLDSLLR 
      ARNITDLYL CGLAYDVCV ASTAKDAIA SGYRTVLLD 
      DCCRGVDLL DIEKTKNFI QENHGLVVD SSQVKEMVE 
      GNDRRPELG LKLALELQQ KRQ
    CDS sequence
    • >TC031161 RA
      atggaattt gtaaagcac acgctactt ttttgttgc 
      tttgctctt tgggagatc gaggccgtg ctgaataaa 
      cgaaacggc ggcgtccaa agctttgtt caaaattac 
      aacgggcat ttcgacacc accatacat aaggtgtct 
      ttcacgcaa aaaggctac atccagttt ttgcgatac 
      ataactcat gtgccaccc ttgagcgac tatacgttc 
      tgcatctgg gtcaggagc cacaacttg acttatgcc 
      catccccta ctttcatat tcgaagcat gaggaagag 
      cgacttatc cgcgtgtgg atttcgccc catggtacc 
      gacattaac ttggaaatc ctccagcac ccaattttc 
      aaaatccac acccacttt gtcgaaaac cactggtac 
      cacgtgtgc cagtcttgg tcctcgtca cacgcctcc 
      tggtcactt tacctcaat ggaaagtta aaatcgagc 
      ggtttcgcg cctcagtta cgaggcagc aaaatcaaa 
      ggcggcggc gatatcgtg gtggggcag gagtacacg 
      gacttcgac aagggattg gacgacggg atcgaaggg 
      gacgttttc gggttcaat ttcgtccta tcaccaacc 
      tcccactcg tcacatggc gacttcccc tcacgcggc 
      cattactcc accgaaatc ccctccgtt ttaagctac 
      ttttcggtt gaaccgaac cgacaaccg aaacttttt 
      gactttttt gaaagtatg tggtcgttt ttcaaccca 
      acgcatgcg attaaagtg gttcccaaa aaaaggggg 
      cactttggg gcaagggtt gccaacgat attgaagag 
      tactcgccc aaaccaaca gggctaaag ttggtagaa 
      ctgagctat cattgtgct ttgggcaaa ggagcgcca 
      gttagtggc aaagatgtg ttgattagt tggacgaaa 
      acgccggtt agggtgttc ggaggggcc attttgaag 
      aatataaag ccattttgc accgatgcg aagggaaat 
      cgtcgtcgc gttgatatt atcgactgt aaacactac 
      ttggcgcgt agttttacc ccaactgca gtagacatg 
      actggtagc acaaatcgc attgatttt gtgaatttt 
      atttgtacg tgcagtatg gacgcgtgc tttgctgct 
      ttcgacaag gattgcgac gggaagttg aacttggag 
      gagttctgg gcgctgtgt cgggcgctt ttccgcaac 
      agcaaaggc gtaacctac gaaatcgaa agacacact 
      ttagagaca attttcaac gtctttgac aagaaccag 
      gacgggagc atcgaccgc gacgagttc gtcacctgc 
      tggaacctc tggatcaaa acaatcgtc aaaccggtc 
      tctgcggcc ctcattatc gacgtgcaa aacgatttt 
      atcaccggg agtctcaac attacgaac tgtccggcc 
      aaacaccac ggtggagat gtcatacaa cccatcaac 
      aatttgctt gaaacggtc gaatttgaa acagtggtg 
      tattcctat gattggcac cccaatgac cacgtctcc 
      ttcattgac aacatctcc ttgagaaaa ctgcacgaa 
      agcagcccc atttcggcc gaaaacgcc aaagtctac 
      gacacggtg gtcttcgac agggagccc ccaatggtc 
      cagaagctg tggccaagg cactgtgtg caaaacacc 
      tggggctcc gacctccac aaggacctc aaggttgtg 
      gaaaactcg accaagatc tacaagggc acaaacccg 
      gacgtggac tcgtattcc ctcttctgg gacaaccag 
      aagctcacc gacactaaa ttagactcg ctgttgagg 
      gccaggaac atcacagat ttgtatttg tgtggcttg 
      gcttacgac gtctgtgtt gcttccaca gccaaagat 
      gcaattgcc agtggctat agaacagtt ctcctcgat 
      gattgttgt cgaggagtc gatctttta gacattgag 
      aaaacgaag aatttcatt caagaaaat cacggtctc 
      gttgtggat tcaagtcaa gttaaggag atggtggaa 
      gggaatgac agaaggccc gagttagga ttaaaatta 
      gctttagaa ttacagcaa aagaggcag tag
    mRNA sequence
    • >TC031161 RA
      aagatgtgc ttgattagt tgaaaaata gagtgtgtt 
      tttcagtgt gatttttta ttcaaataa aaatggaat 
      ttgtaaagc acacgctac ttttttgtt gctttgctc 
      tttgggaga tcgaggccg tgctgaata aacgaaacg 
      gcggcgtcc aaagctttg ttcaaaatt acaacgggc 
      atttcgaca ccaccatac ataaggtgt ctttcacgc 
      aaaaaggct acatccagt ttttgcgat acataactc 
      atgtgccac ccttgagcg actatacgt tctgcatct 
      gggtcagga gccacaact tgacttatg cccatcccc 
      tactttcat attcgaagc atgaggaag agcgactta 
      tccgcgtgt ggatttcgc cccatggta ccgacatta 
      acttggaaa tcctccagc acccaattt tcaaaatcc 
      acacccact ttgtcgaaa accactggt accacgtgt 
      gccagtctt ggtcctcgt cacacgcct cctggtcac 
      tttacctca atggaaagt taaaatcga gcggtttcg 
      cgcctcagt tacgaggca gcaaaatca aaggcggcg 
      gcgatatcg tggtggggc aggagtaca cggacttcg 
      acaagggat tggacgacg ggatcgaag gggacgttt 
      tcgggttca atttcgtcc tatcaccaa cctcccact 
      cgtcacatg gcgacttcc cctcacgcg gccattact 
      ccaccgaaa tcccctccg ttttaagct acttttcgg 
      ttgaaccga accgacaac cgaaacttt ttgactttt 
      ttgaaagta tgtggtcgt ttttcaacc caacgcatg 
      cgattaaag tggttccca aaaaaaggg ggcactttg 
      gggcaaggg ttgccaacg atattgaag agtactcgc 
      ccaaaccaa cagggctaa agttggtag aactgagct 
      atcattgtg ctttgggca aaggagcgc cagttagtg 
      gcaaagatg tgttgatta gttggacga aaacgccgg 
      ttagggtgt tcggagggg ccattttga agaatataa 
      agccatttt gcaccgatg cgaagggaa atcgtcgtc 
      gcgttgata ttatcgact gtaaacact acttggcgc 
      gtagtttta ccccaactg cagtagaca tgactggta 
      gcacaaatc gcattgatt ttgtgaatt ttatttgta 
      cgtgcagta tggacgcgt gctttgctg ctttcgaca 
      aggattgcg acgggaagt tgaacttgg aggagttct 
      gggcgctgt gtcgggcgc ttttccgca acagcaaag 
      gcgtaacct acgaaatcg aaagacaca ctttagaga 
      caattttca acgtctttg acaagaacc aggacggga 
      gcatcgacc gcgacgagt tcgtcacct gctggaacc 
      tctggatca aaacaatcg tcaaaccgg tctctgcgg 
      ccctcatta tcgacgtgc aaaacgatt ttatcaccg 
      ggagtctca acattacga actgtccgg ccaaacacc 
      acggtggag atgtcatac aacccatca acaatttgc 
      ttgaaacgg tcgaatttg aaacagtgg tgtattcct 
      atgattggc accccaatg accacgtct ccttcattg 
      acaacatct ccttgagaa aactgcacg aaagcagcc 
      ccatttcgg ccgaaaacg ccaaagtct acgacacgg 
      tggtcttcg acagggagc ccccaatgg tccagaagc 
      tgtggccaa ggcactgtg tgcaaaaca cctggggct 
      ccgacctcc acaaggacc tcaaggttg tggaaaact 
      cgaccaaga tctacaagg gcacaaacc cggacgtgg 
      actcgtatt ccctcttct gggacaacc agaagctca 
      ccgacacta aattagact cgctgttga gggccagga 
      acatcacag atttgtatt tgtgtggct tggcttacg 
      acgtctgtg ttgcttcca cagccaaag atgcaattg 
      ccagtggct atagaacag ttctcctcg atgattgtt 
      gtcgaggag tcgatcttt tagacattg agaaaacga 
      agaatttca ttcaagaaa atcacggtc tcgttgtgg 
      attcaagtc aagttaagg agatggtgg aagggaatg 
      acagaaggc ccgagttag gattaaaat tagctttag 
      aattacagc aaaagaggc agtagaggt tgcggaatg 
      tggcttcgt acttaattt tctatttat ttgttaaat 
      tattcttct aaatgtagg caaccaact atacgctga 
      ttgattgtt gcaaaaatg tttcaaggt tgtgtaacg 
      ttacgggtg attgcattt aattacgaa taaataagc 
      taatcatct tgacttagt accaataaa aacaattaa 
      agaaattat gatgaaacg caccaatta aacgggcac 
      accgactta gaataaact gtattaatt cat