Status
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Gene information
General information
TC014767 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14818777..14828365
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014767
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02331

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014767 RA
      MWSNNGQMK NNILRHPSS IESKGFLSG NKKWNPRPA 
SWAAPNDVS IFPQAGGRA NPFASKVDP ELIFTKQER 
IGKGSFGEV FKGIDNRTT QVVAIKIID LEEAEDEIE 
DIQQEIMVL SQCDSPYVT KYFGSYLKG TKLWIIMEY 
LGGGSALDL MKAGNFEEM HIAIILREV LKGLDYLHS 
ERKLHRDIK AANVLLSEM GDVKLADFG VAGQLTNTT 
SKRNTFVGT PFWMAPEVI KQSAYDSKA DIWSLGITA 
IELAKGEPP NSELHPMRV LFLIPKNNP PQLTGSYTK 
QFKDFVEAC LNKDPENRP TAKELLKYP FIRKAKKNS 
YLIDLIDRY KKWRCTRNE ESETESENS DSEEPKQES 
DLDDPWIMT VKGNHSIKL PQEDLTPNH KIPNKIQNG 
SGPTIAKSP PKDSNLNHY RPEKATPSK QSVVQSPSD 
NRKDREKRI SRELSPLDH RGGELEIEK KLNRRREHS 
NGGKSDARS PCLSGVIYP LISELQRKH HYNGRAADS 
SHKSNDAIE ELRNAFEIA ERASPGITE SFISEMVKK 
LMPSVTEQR LSSVMEKVT RDAA
    CDS sequence
    • >TC014767 RA
      atgtggagt aataacgga caaatgaaa aacaatatt 
ttaagacac ccttcgtcg atcgaatcg aagggcttc 
ttaagtggt aataaaaag tggaatcct agaccggcc 
tcttgggcc gctcccaac gatgtcagc atttttcca 
caggcgggt ggcagagcc aacccgttc gcatcaaag 
gtcgatcca gaactgata ttcacgaaa caggagcga 
atcggcaaa ggatcattc ggcgaagtg tttaaagga 
atcgacaat cgaacgacg caggtggtc gccatcaag 
atcatagat ttagaagaa gccgaagat gaaatcgaa 
gatatccaa caggaaatt atggtgctg tcacaatgc 
gacagtcct tacgtcacg aaatacttt ggatcgtac 
ctcaaggga accaaacta tggatcatc atggagtac 
ctgggcggc ggttcagca ctggacttg atgaaagct 
gggaatttc gaagagatg cacattgcg atcatcctt 
cgtgaagtc ctcaaaggt ctcgattat ctgcacagt 
gaacggaaa ctgcatcgg gacattaaa gccgccaac 
gttttattg agcgaaatg ggcgatgtg aagttggcc 
gatttcggt gtcgccgga caactcacc aacaccaca 
agcaaacgc aacactttt gttggcacg cctttttgg 
atggcacct gaagtgatt aaacaatcg gcttatgat 
tccaaagcc gacatttgg agtttgggc ataacggcg 
atcgaatta gcaaaaggg gaaccccct aattcagag 
ttacatcca atgcgagtg ttgttcctc attccgaaa 
aataatccg ccacagttg accgggagc tacactaaa 
cagtttaag gacttcgtt gaagcgtgt ctgaataaa 
gatcccgaa aatagaccc acagccaaa gaactgctc 
aaatatcca tttattagg aaagccaag aagaattcg 
tatttaata gacctgatc gataggtac aaaaagtgg 
cgatgtacg aggaacgag gagagcgaa accgaatcg 
gaaaattcg gattcagag gagccgaaa caagagagc 
gatttggac gatccgtgg ataatgacg gtgaaaggc 
aaccattcg attaaatta ccccaagaa gacctaact 
ccaaatcac aagattcca aataaaatt cagaacggt 
tctggacca actatcgcc aaatcgccg ccgaaagat 
tcgaacttg aatcattac cggcctgaa aaggccact 
ccgtcgaaa cagtcggtt gttcagtcg ccttccgat 
aatcggaaa gacagggaa aagaggatt tcgagagag 
ttgagtccg ctggatcat cgaggagga gagttggaa 
atcgagaag aagttgaat cgaaggagg gaacattca 
aacggcggc aaaagcgac gcccgttcg ccatgtcta 
tcaggtgtc atctatcca ctaatatcc gaattgcag 
aggaagcat cactacaac ggccgcgcg gccgatagt 
tctcacaaa tcaaacgat gccattgaa gaattgaga 
aacgcgttt gagatcgca gagagagcg agtccggga 
attacggag agttttatt agcgaaatg gtgaagaag 
ttgatgccg agtgtgacg gagcagagg cttagcagt 
gttatggaa aaagttaca agagatgcg gcgtag
    mRNA sequence
    • >TC014767 RA
      gctcgcgtc gaactttaa aaactcttt tatccttcg 
cattagcat ttcgatcac acacccgat tgatgtttc 
atgctgaat gaattcgac ggaggccca cgtacacgg 
catacgaaa aggcaacac aacagtaca ccggtgcac 
ggcgacaat gtcggtcgt atttcgttg gaagtgaaa 
ggtataata cgctgtggg ttttgtttg gaaacaacg 
attacgttt agtcgtcgt ggacgcgtc gctccagga 
caatgcgcc ggcgaaaat tctagttgc gggcgtcga 
gacgcgtcg tgtaggtgt gtgtgtgtg aataagagg 
gaatttgaa cggaaaaat cgcatccga tgcagttgt 
cacagttag atgacgttg ttccgctct agaaattat 
tttgatctc ctcttcaag tcggtgttt ttcgaaatt 
tcccatgat taagtttgt gttcgccac cgagcatgt 
tggtgataa cggagtgag acagtggtt aacatgtgg 
agtaataac ggacaaatg aaaaacaat attttaaga 
cacccttcg tcgatcgaa tcgaagggc ttcttaagt 
ggtaataaa aagtggaat cctagaccg gcctcttgg 
gccgctccc aacgatgtc agcattttt ccacaggcg 
ggtggcaga gccaacccg ttcgcatca aaggtcgat 
ccagaactg atattcacg aaacaggag cgaatcggc 
aaaggatca ttcggcgaa gtgtttaaa ggaatcgac 
aatcgaacg acgcaggtg gtcgccatc aagatcata 
gatttagaa gaagccgaa gatgaaatc gaagatatc 
caacaggaa attatggtg ctgtcacaa tgcgacagt 
ccttacgtc acgaaatac tttggatcg tacctcaag 
ggaaccaaa ctatggatc atcatggag tacctgggc 
ggcggttca gcactggac ttgatgaaa gctgggaat 
ttcgaagag atgcacatt gcgatcatc cttcgtgaa 
gtcctcaaa ggtctcgat tatctgcac agtgaacgg 
aaactgcat cgggacatt aaagccgcc aacgtttta 
ttgagcgaa atgggcgat gtgaagttg gccgatttc 
ggtgtcgcc ggacaactc accaacacc acaagcaaa 
cgcaacact tttgttggc acgcctttt tggatggca 
cctgaagtg attaaacaa tcggcttat gattccaaa 
gccgacatt tggagtttg ggcataacg gcgatcgaa 
ttagcaaaa ggggaaccc cctaattca gagttacat 
ccaatgcga gtgttgttc ctcattccg aaaaataat 
ccgccacag ttgaccggg agctacact aaacagttt 
aaggacttc gttgaagcg tgtctgaat aaagatccc 
gaaaataga cccacagcc aaagaactg ctcaaatat 
ccatttatt aggaaagcc aagaagaat tcgtattta 
atagacctg atcgatagg tacaaaaag tggcgatgt 
acgaggaac gaggagagc gaaaccgaa tcggaaaat 
tcggattca gaggagccg aaacaagag agcgatttg 
gacgatccg tggataatg acggtgaaa ggcaaccat 
tcgattaaa ttaccccaa gaagaccta actccaaat 
cacaagatt ccaaataaa attcagaac ggttctgga 
ccaactatc gccaaatcg ccgccgaaa gattcgaac 
ttgaatcat taccggcct gaaaaggcc actccgtcg 
aaacagtcg gttgttcag tcgccttcc gataatcgg 
aaagacagg gaaaagagg atttcgaga gagttgagt 
ccgctggat catcgagga ggagagttg gaaatcgag 
aagaagttg aatcgaagg agggaacat tcaaacggc 
ggcaaaagc gacgcccgt tcgccatgt ctatcaggt 
gtcatctat ccactaata tccgaattg cagaggaag 
catcactac aacggccgc gcggccgat agttctcac 
aaatcaaac gatgccatt gaagaattg agaaacgcg 
tttgagatc gcagagaga gcgagtccg ggaattacg 
gagagtttt attagcgaa atggtgaag aagttgatg 
ccgagtgtg acggagcag aggcttagc agtgttatg 
gaaaaagtt acaagagat gcggcgtag gtgagagtt 
agttgagcc agatttgtc ctacatgtt aatacttac 
tgattacat gacaatatc ttatctctt gagcttatt 
acatagcac tggtagatt aagaatttg gaaatcagc 
aaccactgc atgctgttc tgtactcat ttgtggttg 
actttgtca gtttctgaa attatttta acttgttat 
tgccaatgt cccgcggga tgctgtata tatgtatat 
ctagattga attattata tcattaaga taatatttt 
ttcttgcaa tgtattttt ttagatgtg tatatacat 
actatgaac atatagtta ttactttat ttttaattt 
tacggttca gtgtcatcc agcggtcga tttttgttg 
gtgtgcaac aatgattga cttaagact tgtagtagt 
agggatgat ggtttggcg atgtctgag tgtaagtct 
agggtgtcg gttgaaagt gccctttat tcataaact 
agatcacat cagtgtcag atttttatt taatcgatt 
cagttgagt taaaggtgg tttatggcg tattctctg 
ttttggaga gagtgcggg gtttgccca cttcagtat 
tactgtgcg agccgtcag aactcgcat ttgcatgaa 
tgtgtttgc tcaggcttc tgaatccgt tcggaatac 
tgtgataga gtgaacagc atcttatgc gagtcttta 
ttcttattg aaacgcttt aatgttgaa ctgttcatg 
tcaactact tattgttga ttttaattg ttatcttat 
ttagagaat gaatcttat actttccgc atagttata 
ctgtacagt caattacaa atgactttg tattctaga 
ctaacctgt aatttaaca tggcaaatt acaacaaat 
ctgtatgag atctgcttg tcttgtaca tggtgtaca 
ttaataaaa ttatttcaa tttc

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY