| General information | |||
| TC014767 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 14818777..14828365 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014767 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02331 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014767 RA
MWSNNGQMK NNILRHPSS IESKGFLSG NKKWNPRPA SWAAPNDVS IFPQAGGRA NPFASKVDP ELIFTKQER IGKGSFGEV FKGIDNRTT QVVAIKIID LEEAEDEIE DIQQEIMVL SQCDSPYVT KYFGSYLKG TKLWIIMEY LGGGSALDL MKAGNFEEM HIAIILREV LKGLDYLHS ERKLHRDIK AANVLLSEM GDVKLADFG VAGQLTNTT SKRNTFVGT PFWMAPEVI KQSAYDSKA DIWSLGITA IELAKGEPP NSELHPMRV LFLIPKNNP PQLTGSYTK QFKDFVEAC LNKDPENRP TAKELLKYP FIRKAKKNS YLIDLIDRY KKWRCTRNE ESETESENS DSEEPKQES DLDDPWIMT VKGNHSIKL PQEDLTPNH KIPNKIQNG SGPTIAKSP PKDSNLNHY RPEKATPSK QSVVQSPSD NRKDREKRI SRELSPLDH RGGELEIEK KLNRRREHS NGGKSDARS PCLSGVIYP LISELQRKH HYNGRAADS SHKSNDAIE ELRNAFEIA ERASPGITE SFISEMVKK LMPSVTEQR LSSVMEKVT RDAA
- >TC014767 RA
atgtggagt aataacgga caaatgaaa aacaatatt ttaagacac ccttcgtcg atcgaatcg aagggcttc ttaagtggt aataaaaag tggaatcct agaccggcc tcttgggcc gctcccaac gatgtcagc atttttcca caggcgggt ggcagagcc aacccgttc gcatcaaag gtcgatcca gaactgata ttcacgaaa caggagcga atcggcaaa ggatcattc ggcgaagtg tttaaagga atcgacaat cgaacgacg caggtggtc gccatcaag atcatagat ttagaagaa gccgaagat gaaatcgaa gatatccaa caggaaatt atggtgctg tcacaatgc gacagtcct tacgtcacg aaatacttt ggatcgtac ctcaaggga accaaacta tggatcatc atggagtac ctgggcggc ggttcagca ctggacttg atgaaagct gggaatttc gaagagatg cacattgcg atcatcctt cgtgaagtc ctcaaaggt ctcgattat ctgcacagt gaacggaaa ctgcatcgg gacattaaa gccgccaac gttttattg agcgaaatg ggcgatgtg aagttggcc gatttcggt gtcgccgga caactcacc aacaccaca agcaaacgc aacactttt gttggcacg cctttttgg atggcacct gaagtgatt aaacaatcg gcttatgat tccaaagcc gacatttgg agtttgggc ataacggcg atcgaatta gcaaaaggg gaaccccct aattcagag ttacatcca atgcgagtg ttgttcctc attccgaaa aataatccg ccacagttg accgggagc tacactaaa cagtttaag gacttcgtt gaagcgtgt ctgaataaa gatcccgaa aatagaccc acagccaaa gaactgctc aaatatcca tttattagg aaagccaag aagaattcg tatttaata gacctgatc gataggtac aaaaagtgg cgatgtacg aggaacgag gagagcgaa accgaatcg gaaaattcg gattcagag gagccgaaa caagagagc gatttggac gatccgtgg ataatgacg gtgaaaggc aaccattcg attaaatta ccccaagaa gacctaact ccaaatcac aagattcca aataaaatt cagaacggt tctggacca actatcgcc aaatcgccg ccgaaagat tcgaacttg aatcattac cggcctgaa aaggccact ccgtcgaaa cagtcggtt gttcagtcg ccttccgat aatcggaaa gacagggaa aagaggatt tcgagagag ttgagtccg ctggatcat cgaggagga gagttggaa atcgagaag aagttgaat cgaaggagg gaacattca aacggcggc aaaagcgac gcccgttcg ccatgtcta tcaggtgtc atctatcca ctaatatcc gaattgcag aggaagcat cactacaac ggccgcgcg gccgatagt tctcacaaa tcaaacgat gccattgaa gaattgaga aacgcgttt gagatcgca gagagagcg agtccggga attacggag agttttatt agcgaaatg gtgaagaag ttgatgccg agtgtgacg gagcagagg cttagcagt gttatggaa aaagttaca agagatgcg gcgtag
- >TC014767 RA
gctcgcgtc gaactttaa aaactcttt tatccttcg cattagcat ttcgatcac acacccgat tgatgtttc atgctgaat gaattcgac ggaggccca cgtacacgg catacgaaa aggcaacac aacagtaca ccggtgcac ggcgacaat gtcggtcgt atttcgttg gaagtgaaa ggtataata cgctgtggg ttttgtttg gaaacaacg attacgttt agtcgtcgt ggacgcgtc gctccagga caatgcgcc ggcgaaaat tctagttgc gggcgtcga gacgcgtcg tgtaggtgt gtgtgtgtg aataagagg gaatttgaa cggaaaaat cgcatccga tgcagttgt cacagttag atgacgttg ttccgctct agaaattat tttgatctc ctcttcaag tcggtgttt ttcgaaatt tcccatgat taagtttgt gttcgccac cgagcatgt tggtgataa cggagtgag acagtggtt aacatgtgg agtaataac ggacaaatg aaaaacaat attttaaga cacccttcg tcgatcgaa tcgaagggc ttcttaagt ggtaataaa aagtggaat cctagaccg gcctcttgg gccgctccc aacgatgtc agcattttt ccacaggcg ggtggcaga gccaacccg ttcgcatca aaggtcgat ccagaactg atattcacg aaacaggag cgaatcggc aaaggatca ttcggcgaa gtgtttaaa ggaatcgac aatcgaacg acgcaggtg gtcgccatc aagatcata gatttagaa gaagccgaa gatgaaatc gaagatatc caacaggaa attatggtg ctgtcacaa tgcgacagt ccttacgtc acgaaatac tttggatcg tacctcaag ggaaccaaa ctatggatc atcatggag tacctgggc ggcggttca gcactggac ttgatgaaa gctgggaat ttcgaagag atgcacatt gcgatcatc cttcgtgaa gtcctcaaa ggtctcgat tatctgcac agtgaacgg aaactgcat cgggacatt aaagccgcc aacgtttta ttgagcgaa atgggcgat gtgaagttg gccgatttc ggtgtcgcc ggacaactc accaacacc acaagcaaa cgcaacact tttgttggc acgcctttt tggatggca cctgaagtg attaaacaa tcggcttat gattccaaa gccgacatt tggagtttg ggcataacg gcgatcgaa ttagcaaaa ggggaaccc cctaattca gagttacat ccaatgcga gtgttgttc ctcattccg aaaaataat ccgccacag ttgaccggg agctacact aaacagttt aaggacttc gttgaagcg tgtctgaat aaagatccc gaaaataga cccacagcc aaagaactg ctcaaatat ccatttatt aggaaagcc aagaagaat tcgtattta atagacctg atcgatagg tacaaaaag tggcgatgt acgaggaac gaggagagc gaaaccgaa tcggaaaat tcggattca gaggagccg aaacaagag agcgatttg gacgatccg tggataatg acggtgaaa ggcaaccat tcgattaaa ttaccccaa gaagaccta actccaaat cacaagatt ccaaataaa attcagaac ggttctgga ccaactatc gccaaatcg ccgccgaaa gattcgaac ttgaatcat taccggcct gaaaaggcc actccgtcg aaacagtcg gttgttcag tcgccttcc gataatcgg aaagacagg gaaaagagg atttcgaga gagttgagt ccgctggat catcgagga ggagagttg gaaatcgag aagaagttg aatcgaagg agggaacat tcaaacggc ggcaaaagc gacgcccgt tcgccatgt ctatcaggt gtcatctat ccactaata tccgaattg cagaggaag catcactac aacggccgc gcggccgat agttctcac aaatcaaac gatgccatt gaagaattg agaaacgcg tttgagatc gcagagaga gcgagtccg ggaattacg gagagtttt attagcgaa atggtgaag aagttgatg ccgagtgtg acggagcag aggcttagc agtgttatg gaaaaagtt acaagagat gcggcgtag gtgagagtt agttgagcc agatttgtc ctacatgtt aatacttac tgattacat gacaatatc ttatctctt gagcttatt acatagcac tggtagatt aagaatttg gaaatcagc aaccactgc atgctgttc tgtactcat ttgtggttg actttgtca gtttctgaa attatttta acttgttat tgccaatgt cccgcggga tgctgtata tatgtatat ctagattga attattata tcattaaga taatatttt ttcttgcaa tgtattttt ttagatgtg tatatacat actatgaac atatagtta ttactttat ttttaattt tacggttca gtgtcatcc agcggtcga tttttgttg gtgtgcaac aatgattga cttaagact tgtagtagt agggatgat ggtttggcg atgtctgag tgtaagtct agggtgtcg gttgaaagt gccctttat tcataaact agatcacat cagtgtcag atttttatt taatcgatt cagttgagt taaaggtgg tttatggcg tattctctg ttttggaga gagtgcggg gtttgccca cttcagtat tactgtgcg agccgtcag aactcgcat ttgcatgaa tgtgtttgc tcaggcttc tgaatccgt tcggaatac tgtgataga gtgaacagc atcttatgc gagtcttta ttcttattg aaacgcttt aatgttgaa ctgttcatg tcaactact tattgttga ttttaattg ttatcttat ttagagaat gaatcttat actttccgc atagttata ctgtacagt caattacaa atgactttg tattctaga ctaacctgt aatttaaca tggcaaatt acaacaaat ctgtatgag atctgcttg tcttgtaca tggtgtaca ttaataaaa ttatttcaa tttc
