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Gene information
General information
TC014767 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14818777..14828365
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014767
    iBeetle screen iB_02331

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014767 RA
      MWSNNGQMK NNILRHPSS IESKGFLSG NKKWNPRPA 
      SWAAPNDVS IFPQAGGRA NPFASKVDP ELIFTKQER 
      IGKGSFGEV FKGIDNRTT QVVAIKIID LEEAEDEIE 
      DIQQEIMVL SQCDSPYVT KYFGSYLKG TKLWIIMEY 
      LGGGSALDL MKAGNFEEM HIAIILREV LKGLDYLHS 
      ERKLHRDIK AANVLLSEM GDVKLADFG VAGQLTNTT 
      SKRNTFVGT PFWMAPEVI KQSAYDSKA DIWSLGITA 
      IELAKGEPP NSELHPMRV LFLIPKNNP PQLTGSYTK 
      QFKDFVEAC LNKDPENRP TAKELLKYP FIRKAKKNS 
      YLIDLIDRY KKWRCTRNE ESETESENS DSEEPKQES 
      DLDDPWIMT VKGNHSIKL PQEDLTPNH KIPNKIQNG 
      SGPTIAKSP PKDSNLNHY RPEKATPSK QSVVQSPSD 
      NRKDREKRI SRELSPLDH RGGELEIEK KLNRRREHS 
      NGGKSDARS PCLSGVIYP LISELQRKH HYNGRAADS 
      SHKSNDAIE ELRNAFEIA ERASPGITE SFISEMVKK 
      LMPSVTEQR LSSVMEKVT RDAA
    CDS sequence
    • >TC014767 RA
      atgtggagt aataacgga caaatgaaa aacaatatt 
      ttaagacac ccttcgtcg atcgaatcg aagggcttc 
      ttaagtggt aataaaaag tggaatcct agaccggcc 
      tcttgggcc gctcccaac gatgtcagc atttttcca 
      caggcgggt ggcagagcc aacccgttc gcatcaaag 
      gtcgatcca gaactgata ttcacgaaa caggagcga 
      atcggcaaa ggatcattc ggcgaagtg tttaaagga 
      atcgacaat cgaacgacg caggtggtc gccatcaag 
      atcatagat ttagaagaa gccgaagat gaaatcgaa 
      gatatccaa caggaaatt atggtgctg tcacaatgc 
      gacagtcct tacgtcacg aaatacttt ggatcgtac 
      ctcaaggga accaaacta tggatcatc atggagtac 
      ctgggcggc ggttcagca ctggacttg atgaaagct 
      gggaatttc gaagagatg cacattgcg atcatcctt 
      cgtgaagtc ctcaaaggt ctcgattat ctgcacagt 
      gaacggaaa ctgcatcgg gacattaaa gccgccaac 
      gttttattg agcgaaatg ggcgatgtg aagttggcc 
      gatttcggt gtcgccgga caactcacc aacaccaca 
      agcaaacgc aacactttt gttggcacg cctttttgg 
      atggcacct gaagtgatt aaacaatcg gcttatgat 
      tccaaagcc gacatttgg agtttgggc ataacggcg 
      atcgaatta gcaaaaggg gaaccccct aattcagag 
      ttacatcca atgcgagtg ttgttcctc attccgaaa 
      aataatccg ccacagttg accgggagc tacactaaa 
      cagtttaag gacttcgtt gaagcgtgt ctgaataaa 
      gatcccgaa aatagaccc acagccaaa gaactgctc 
      aaatatcca tttattagg aaagccaag aagaattcg 
      tatttaata gacctgatc gataggtac aaaaagtgg 
      cgatgtacg aggaacgag gagagcgaa accgaatcg 
      gaaaattcg gattcagag gagccgaaa caagagagc 
      gatttggac gatccgtgg ataatgacg gtgaaaggc 
      aaccattcg attaaatta ccccaagaa gacctaact 
      ccaaatcac aagattcca aataaaatt cagaacggt 
      tctggacca actatcgcc aaatcgccg ccgaaagat 
      tcgaacttg aatcattac cggcctgaa aaggccact 
      ccgtcgaaa cagtcggtt gttcagtcg ccttccgat 
      aatcggaaa gacagggaa aagaggatt tcgagagag 
      ttgagtccg ctggatcat cgaggagga gagttggaa 
      atcgagaag aagttgaat cgaaggagg gaacattca 
      aacggcggc aaaagcgac gcccgttcg ccatgtcta 
      tcaggtgtc atctatcca ctaatatcc gaattgcag 
      aggaagcat cactacaac ggccgcgcg gccgatagt 
      tctcacaaa tcaaacgat gccattgaa gaattgaga 
      aacgcgttt gagatcgca gagagagcg agtccggga 
      attacggag agttttatt agcgaaatg gtgaagaag 
      ttgatgccg agtgtgacg gagcagagg cttagcagt 
      gttatggaa aaagttaca agagatgcg gcgtag
    mRNA sequence
    • >TC014767 RA
      gctcgcgtc gaactttaa aaactcttt tatccttcg 
      cattagcat ttcgatcac acacccgat tgatgtttc 
      atgctgaat gaattcgac ggaggccca cgtacacgg 
      catacgaaa aggcaacac aacagtaca ccggtgcac 
      ggcgacaat gtcggtcgt atttcgttg gaagtgaaa 
      ggtataata cgctgtggg ttttgtttg gaaacaacg 
      attacgttt agtcgtcgt ggacgcgtc gctccagga 
      caatgcgcc ggcgaaaat tctagttgc gggcgtcga 
      gacgcgtcg tgtaggtgt gtgtgtgtg aataagagg 
      gaatttgaa cggaaaaat cgcatccga tgcagttgt 
      cacagttag atgacgttg ttccgctct agaaattat 
      tttgatctc ctcttcaag tcggtgttt ttcgaaatt 
      tcccatgat taagtttgt gttcgccac cgagcatgt 
      tggtgataa cggagtgag acagtggtt aacatgtgg 
      agtaataac ggacaaatg aaaaacaat attttaaga 
      cacccttcg tcgatcgaa tcgaagggc ttcttaagt 
      ggtaataaa aagtggaat cctagaccg gcctcttgg 
      gccgctccc aacgatgtc agcattttt ccacaggcg 
      ggtggcaga gccaacccg ttcgcatca aaggtcgat 
      ccagaactg atattcacg aaacaggag cgaatcggc 
      aaaggatca ttcggcgaa gtgtttaaa ggaatcgac 
      aatcgaacg acgcaggtg gtcgccatc aagatcata 
      gatttagaa gaagccgaa gatgaaatc gaagatatc 
      caacaggaa attatggtg ctgtcacaa tgcgacagt 
      ccttacgtc acgaaatac tttggatcg tacctcaag 
      ggaaccaaa ctatggatc atcatggag tacctgggc 
      ggcggttca gcactggac ttgatgaaa gctgggaat 
      ttcgaagag atgcacatt gcgatcatc cttcgtgaa 
      gtcctcaaa ggtctcgat tatctgcac agtgaacgg 
      aaactgcat cgggacatt aaagccgcc aacgtttta 
      ttgagcgaa atgggcgat gtgaagttg gccgatttc 
      ggtgtcgcc ggacaactc accaacacc acaagcaaa 
      cgcaacact tttgttggc acgcctttt tggatggca 
      cctgaagtg attaaacaa tcggcttat gattccaaa 
      gccgacatt tggagtttg ggcataacg gcgatcgaa 
      ttagcaaaa ggggaaccc cctaattca gagttacat 
      ccaatgcga gtgttgttc ctcattccg aaaaataat 
      ccgccacag ttgaccggg agctacact aaacagttt 
      aaggacttc gttgaagcg tgtctgaat aaagatccc 
      gaaaataga cccacagcc aaagaactg ctcaaatat 
      ccatttatt aggaaagcc aagaagaat tcgtattta 
      atagacctg atcgatagg tacaaaaag tggcgatgt 
      acgaggaac gaggagagc gaaaccgaa tcggaaaat 
      tcggattca gaggagccg aaacaagag agcgatttg 
      gacgatccg tggataatg acggtgaaa ggcaaccat 
      tcgattaaa ttaccccaa gaagaccta actccaaat 
      cacaagatt ccaaataaa attcagaac ggttctgga 
      ccaactatc gccaaatcg ccgccgaaa gattcgaac 
      ttgaatcat taccggcct gaaaaggcc actccgtcg 
      aaacagtcg gttgttcag tcgccttcc gataatcgg 
      aaagacagg gaaaagagg atttcgaga gagttgagt 
      ccgctggat catcgagga ggagagttg gaaatcgag 
      aagaagttg aatcgaagg agggaacat tcaaacggc 
      ggcaaaagc gacgcccgt tcgccatgt ctatcaggt 
      gtcatctat ccactaata tccgaattg cagaggaag 
      catcactac aacggccgc gcggccgat agttctcac 
      aaatcaaac gatgccatt gaagaattg agaaacgcg 
      tttgagatc gcagagaga gcgagtccg ggaattacg 
      gagagtttt attagcgaa atggtgaag aagttgatg 
      ccgagtgtg acggagcag aggcttagc agtgttatg 
      gaaaaagtt acaagagat gcggcgtag gtgagagtt 
      agttgagcc agatttgtc ctacatgtt aatacttac 
      tgattacat gacaatatc ttatctctt gagcttatt 
      acatagcac tggtagatt aagaatttg gaaatcagc 
      aaccactgc atgctgttc tgtactcat ttgtggttg 
      actttgtca gtttctgaa attatttta acttgttat 
      tgccaatgt cccgcggga tgctgtata tatgtatat 
      ctagattga attattata tcattaaga taatatttt 
      ttcttgcaa tgtattttt ttagatgtg tatatacat 
      actatgaac atatagtta ttactttat ttttaattt 
      tacggttca gtgtcatcc agcggtcga tttttgttg 
      gtgtgcaac aatgattga cttaagact tgtagtagt 
      agggatgat ggtttggcg atgtctgag tgtaagtct 
      agggtgtcg gttgaaagt gccctttat tcataaact 
      agatcacat cagtgtcag atttttatt taatcgatt 
      cagttgagt taaaggtgg tttatggcg tattctctg 
      ttttggaga gagtgcggg gtttgccca cttcagtat 
      tactgtgcg agccgtcag aactcgcat ttgcatgaa 
      tgtgtttgc tcaggcttc tgaatccgt tcggaatac 
      tgtgataga gtgaacagc atcttatgc gagtcttta 
      ttcttattg aaacgcttt aatgttgaa ctgttcatg 
      tcaactact tattgttga ttttaattg ttatcttat 
      ttagagaat gaatcttat actttccgc atagttata 
      ctgtacagt caattacaa atgactttg tattctaga 
      ctaacctgt aatttaaca tggcaaatt acaacaaat 
      ctgtatgag atctgcttg tcttgtaca tggtgtaca 
      ttaataaaa ttatttcaa tttc
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