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Gene information
General information
TC014752 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 6580749..6589714
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014752
    iBeetle screen iB_02330
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014752 RA
      MASSRKKQD EKNLKTLRE LGAQPQNKY CFDCNQRGP 
      TYVNMTIGS FVCTKCSGM LRGLTPPHR VKSISMATF 
      TSEEIELLK NRGNDYCKS VWLGLYDGT LPGAEYRDE 
      QAVKDFMVD KYERKRYYM EPAQALKNG YTKTEKVKP 
      LTTLVSDPK PIKLNGNAT EFNRKRPEP GKNHSFAVD 
      FDNADIFAS NNNNTTTTQ ETFANFDDN PVFNSKMNN 
      ASMPTPTFD NFNLAQSNK VPAAPPEDK YAALKDLDN 
      ALKTQNAVD WNSSGSNGS LYSSPTPTG SMYSSPSPQ 
      SSMFGSPSQ GQFMNAFQN QDTFSNPFN GMNWGSNGN 
      FGQNLANPF REPPVRVNG FAQNFQPMF PVAANGGAW 
      GANPFKVGA TANVNSNNP FL
    CDS sequence
    • >TC014752 RA
      atggcttcg tcacgaaaa aagcaagat gagaagaac 
      ttaaaaacg ttacgcgaa ctgggggcg cagccccag 
      aacaagtac tgtttcgac tgcaaccag cgcggcccc 
      acgtacgtc aacatgaca atcggctcg tttgtgtgc 
      accaagtgc tccggaatg ctacgcggt ttgacgccg 
      ccacatcgc gtcaaatcg atctcgatg gccacattc 
      acgtcggaa gagatcgaa ctgttgaaa aatcgcggc 
      aacgactac tgcaagagt gtgtggctg ggcttgtac 
      gatggcact ttaccgggg gccgagtac agggacgaa 
      caggccgtc aaggacttc atggtcgat aaatacgaa 
      aggaagagg tattacatg gagccggcc caagccctc 
      aagaacgga tacacgaaa acggaaaaa gtgaaaccg 
      ctgacgacg ctcgtttcg gacccgaaa ccgatcaaa 
      ttgaacgga aacgcgacg gaatttaat agaaaaagg 
      ccagagccc ggcaagaat cattcgttt gctgtcgat 
      ttcgacaat gctgatatc ttcgcttcc aacaacaat 
      aacacaacg acaacgcaa gagactttt gccaatttt 
      gacgataat cccgttttc aacagcaaa atgaacaat 
      gcaagtatg ccgacgccg actttcgac aattttaat 
      ttagcacaa tcaaacaaa gtacctgca gccccgccc 
      gaagacaag tacgccgcc ctcaaagac ctcgataac 
      gctttaaag acgcaaaat gcagtcgat tggaattcg 
      tcaggttcc aatgggtcg ttgtacagc tctccaaca 
      cccaccggc tccatgtac agttcacca tctccgcaa 
      agttccatg tttgggtcg cctagtcaa ggtcagttt 
      atgaacgcg ttccagaat caggatacg ttctcgaat 
      cccttcaat ggcatgaat tgggggagt aatggaaat 
      ttcggacag aatctcgcc aatcccttc cgtgaaccg 
      ccggttaga gtcaacggt ttcgcccag aacttccag 
      cccatgttc ccggtcgct gccaacggg ggcgcatgg 
      ggggccaac cccttcaag gtgggtgcc acggctaat 
      gtgaattcc aacaatcca tttttatga
    mRNA sequence
    • >TC014752 RA
      tgcaaaaac caaaaattt tcaaaaaat ccaaaatat 
      ttcaaaaac ggctaaatt tacgtgtag agaaagctg 
      atgagtaat gaaagtccc cttgaatcg gagcagcgt 
      gtataagag tacgatgtt aatataaaa ttctctagt 
      ctatagttg taatgtgga ataagttag tgtctaaaa 
      atgaacaat gtgaagact ctgcaaggt gctaaaata 
      ctgacgttt tcagtaaat tttgttgtt tcttccttc 
      tgatattta aagtccgtt attgtggat ttgcaattt 
      cgctgaaac taactcagt tcttgccct actcactta 
      aaaaacgat gtcagaact agtgggtac atcaggaat 
      gtttcttct tccactctg tgtaattac ggaaaatga 
      ctattgtat attttctag tggtagtgc ctcactgtt 
      ctaatcatt ttgatacat agttaggaa caatataga 
      ctgacgatt tatagctta gttctcgtt aaattcagc 
      caactcctt ggtatatct gtgtacata gtccccttg 
      ttggccaat taaattgtt aatatatta tgcaaagtg 
      atttcacaa atcataaat atatcatat tacgactga 
      gcgtgtttt taactgcgc aaatccgat taccggaag 
      caatttaat ccccgaaat ccattgacc ccccttatc 
      atttccgaa aattgacgt tcgagcttt gtggaatcg 
      ccactgcag gcgttgacg gcgcctcag ctgtcacac 
      aattgtcag ttgtgccag tgttgtgga gcagaatgg 
      cttcgtcac gaaaaaagc aagatgaga agaacttaa 
      aaacgttac gcgaactgg gggcgcagc cccagaaca 
      agtactgtt tcgactgca accagcgcg gccccacgt 
      acgtcaaca tgacaatcg gctcgtttg tgtgcacca 
      agtgctccg gaatgctac gcggtttga cgccgccac 
      atcgcgtca aatcgatct cgatggcca cattcacgt 
      cggaagaga tcgaactgt tgaaaaatc gcggcaacg 
      actactgca agagtgtgt ggctgggct tgtacgatg 
      gcactttac cgggggccg agtacaggg acgaacagg 
      ccgtcaagg acttcatgg tcgataaat acgaaagga 
      agaggtatt acatggagc cggcccaag ccctcaaga 
      acggataca cgaaaacgg aaaaagtga aaccgctga 
      cgacgctcg tttcggacc cgaaaccga tcaaattga 
      acggaaacg cgacggaat ttaatagaa aaaggccag 
      agcccggca agaatcatt cgtttgctg tcgatttcg 
      acaatgctg atatcttcg cttccaaca acaataaca 
      caacgacaa cgcaagaga cttttgcca attttgacg 
      ataatcccg ttttcaaca gcaaaatga acaatgcaa 
      gtatgccga cgccgactt tcgacaatt ttaatttag 
      cacaatcaa acaaagtac ctgcagccc cgcccgaag 
      acaagtacg ccgccctca aagacctcg ataacgctt 
      taaagacgc aaaatgcag tcgattgga attcgtcag 
      gttccaatg ggtcgttgt acagctctc caacaccca 
      ccggctcca tgtacagtt caccatctc cgcaaagtt 
      ccatgtttg ggtcgccta gtcaaggtc agtttatga 
      acgcgttcc agaatcagg atacgttct cgaatccct 
      tcaatggca tgaattggg ggagtaatg gaaatttcg 
      gacagaatc tcgccaatc ccttccgtg aaccgccgg 
      ttagagtca acggtttcg cccagaact tccagccca 
      tgttcccgg tcgctgcca acgggggcg catgggggg 
      ccaacccct tcaaggtgg gtgccacgg ctaatgtga 
      attccaaca atccatttt tatgagttg tgtagtcaa 
      cgagcaacg taattttaa tctgcttta agtttctct 
      ctttctctc tgccgattg gacaagttc cgttattta 
      tagtagaag aatccacaa gtagtgaag tgtacgtaa 
      attatttct tcctttgaa ttaatccac caactgatc 
      tatttattt attttttgc ctattataa caatgtatc 
      aaagtcaaa accaaaaat gctttttgt cttttataa 
      tatagctac gtaaactaa gaatgtaac gttaagtaa 
      tgttaaagt agtagttaa tacgcttac aagaaatca 
      aaccgttag gttgccttc tgttatgat gaacaacta 
      gaatgaaca ataattttg tgtatattt atttatcgt 
      gcgttggac tttttttaa ttggaaagt ggccttgat 
      aggtttacg agactgtct tttttaata tgtgtaacg 
      atagcttct tgattttgt tttgtaata aaactattc 
      ttaattgat tgacgatgt tctgtgata aaagatgct 
      tcaatgcaa atcaacttg tattagtga aaatcgaaa 
      tttggtgtt ttttttttc tctcctccg ttacatctc 
      gtgaacgat gtgaaacag actctccgt cccggttaa 
      aataataat attaagtcg agcacaatt tatgcaatg 
      tatattgtt gtcaaaagg ttgtgtaaa tacataaag 
      aaataacac