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Gene information
General information
TC014715 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 5873278..5890446
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014715
    iBeetle screen iB_02328
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    club colour lighter - Number of animals showing the phenotype: 5


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014715 RA
      MMKHVQLSP YRSRADTVS LSSTTSYGS LSPEPLGSR 
      SSSYSSLND SSSGNLQTT IKVYAKCLR PDIEYKTLS 
      ITFQTTCKE VVATLLGKY KMKHRDPKL FYLSMEVTV 
      RKAGVRTHL ALDEEARPA VLQSCHPRG DSKFSLQTR 
      RGGLVKVHD SVLNSNSQY KSLLISART TVDELIALL 
      LNCYNSKER VEQFSLYEV NTDKEQQRK LHPDDRPLQ 
      VQQTWLPLH KCHFLVRRN PDYLPLSRR KIFWVSDLP 
      PLPQSRLFQ SATQLTQIS KSQNSLVPT NTKEETKTK 
      TAQLKSSKE YVSRNKNSD LKVFGSSSQ IAQNGSNSY 
      ADYENYFYI
    CDS sequence
    • >TC014715 RA
      atgatgaaa cacgtgcag ctttctcct tacaggagc 
      agagctgac acagtgtcc ctaagctca acgacatcc 
      tacggaagt ctcagtcct gaaccgcta ggaagtcgc 
      tcttcgagc tactcgtca ctcaatgac tcttcttca 
      ggaaattta cagacaacc ataaaagtt tatgccaaa 
      tgtttacga ccggatatc gagtacaag accctcagc 
      atcactttc caaacgacg tgtaaagaa gtagtagcg 
      acacttttg ggcaaatat aaaatgaag caccgagac 
      ccgaaactt ttctatctc agtatggaa gttacggtt 
      cgtaaagcc ggagttcga acacatctg gctttagat 
      gaggaagcg agacccgcg gtgttacaa agttgccac 
      ccaagaggc gattcgaag ttttccctc cagactcga 
      cgcggaggc ttggtaaaa gtccacgat tcggtcctc 
      aacagcaac tcgcagtat aaaagtcta ttaataagc 
      gctaggaca acagtagat gaattaata gccctgctt 
      ttgaattgt tacaacagc aaagaacgc gtggagcag 
      ttctctttg tacgaagtc aacactgat aaggagcaa 
      caaagaaaa ttacatccg gacgatagg cccctgcag 
      gtgcaacaa acttggtta cccttacac aaatgccat 
      ttcctagtc agaagaaac cccgactat ttaccgctc 
      tcgcgaaga aaaattttc tgggtctct gacttgccg 
      ccgttacct cagtccagg cttttccaa tcagccact 
      caactcacc caaatctct aagtcacag aactctctg 
      gttccgaca aacacgaag gaagaaacc aagactaaa 
      acagctcaa ctcaagtcg agcaaagag tacgtcagt 
      aggaacaaa aacagcgat ttgaaagtt ttcggaagc 
      agttcacaa atcgcacaa aacggaagc aactcgtat 
      gcggattat gagaattat ttttacatt tga
    mRNA sequence
    • >TC014715 RA
      cagcaagaa acaacggaa tttctctcc caacaccca 
      ccctgaagg aaagtgtga gaatacgaa aagcctcca 
      aacaataac actacaatg aacctgcct gcatcccac 
      tatcatcca taatctgat agaaatgca ttttatggt 
      gtctttggg acaagccga ggcagagta acgcgactc 
      aaccgtggg ttaaagctc gcagctatc agtcgcgtt 
      ttaacgttg gtgttggaa ggacgtggt gttggaaca 
      cttcggcgc agctccatg tgctgcaca cgtttctga 
      gtgcgtgtt ttcgtgaac ttttcccag ttgctgatt 
      tttgggatt accattgac tgaagggat taccaagat 
      gatgaaaca cgtgcagct ttctcctta caggagcag 
      agctgacac agtgtccct aagctcaac gacatccta 
      cggaagtct cagtcctga accgctagg aagtcgctc 
      ttcgagcta ctcgtcact caatgactc ttcttcagg 
      aaatttaca gacaaccat aaaagttta tgccaaatg 
      tttacgacc ggatatcga gtacaagac cctcagcat 
      cactttcca aacgacgtg taaagaagt agtagcgac 
      acttttggg caaatataa aatgaagca ccgagaccc 
      gaaactttt ctatctcag tatggaagt tacggttcg 
      taaagccgg agttcgaac acatctggc tttagatga 
      ggaagcgag acccgcggt gttacaaag ttgccaccc 
      aagaggcga ttcgaagtt ttccctcca gactcgacg 
      cggaggctt ggtaaaagt ccacgattc ggtcctcaa 
      cagcaactc gcagtataa aagtctatt aataagcgc 
      taggacaac agtagatga attaatagc cctgctttt 
      gaattgtta caacagcaa agaacgcgt ggagcagtt 
      ctctttgta cgaagtcaa cactgataa ggagcaaca 
      aagaaaatt acatccgga cgataggcc cctgcaggt 
      gcaacaaac ttggttacc cttacacaa atgccattt 
      cctagtcag aagaaaccc cgactattt accgctctc 
      gcgaagaaa aattttctg ggtctctga cttgccgcc 
      gttacctca gtccaggct tttccaatc agccactca 
      actcaccca aatctctaa gtcacagaa ctctctggt 
      tccgacaaa cacgaagga agaaaccaa gactaaaac 
      agctcaact caagtcgag caaagagta cgtcagtag 
      gaacaaaaa cagcgattt gaaagtttt cggaagcag 
      ttcacaaat cgcacaaaa cggaagcaa ctcgtatgc 
      ggattatga gaattattt ttacatttg aaatttttt 
      ttgttcctg tgtgagtct agttggttt ttggatgtg 
      atagatgca gcctaaaat tgattattt taaccaaag 
      atttccaga aagtctctc ttttaaatt tgaattttg 
      ttcgtcttg aagtaataa atgtttgag tgtaataat 
      ttgtggact atttggagt agttttata atcatacct 
      gattagcgc acagcgtct atttattgt ttgatagat 
      taattgtgt aatgagttt tttccttat gtgtgtagt 
      taaactaaa tgttaatct attcgtacg tctgatgtg 
      tttacccaa agactagaa ttaaaacat aattttgga 
      aacgtatct gtataacta actgtgcaa tgtgtatat 
      aaaaatacc aaagaatca ctgtaaata cctttgtat 
      gtattttaa tttaaggtt ttagaccat tttgaataa 
      aggaagtta aaaaag