Status
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Gene information
General information
TC014700 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13553080..13557278
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014700
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02324

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 12.5% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 25.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014700 RA
      MDESLSGKK KSSWWEKRT RYEKCLIVL TGLCIIVII 
      YLASNLLMR FPASTEHLK KDVCMTEKC IRSASSILE 
      KMDRTVDPC DDFYKFACG NFVEKQIIP DHKTMVSSF 
      GETSDKVSL QLLKVFEED IHEKDPHSL RLVKSIYKS 
      CMNTSQIEN EGLSFMKRE FRDLGGWPV IEPNWNEKT 
      FDWKNLLFR LKRIGWRRT FFLSMFIDT DLKNSSKRI 
      FTISQPCMP YDRYKKGFN DTAVKAGYE YLVELAVLF 
      GADRTAARA EMREVVEFQ IELAKMIIP YEERKNVSL 
      SYNPVTIRE LQRNFTTIP WLKLINNYL SPIMLTSDT 
      VVNVAVPTF LERLENFLP TVKKRVLAN YMFTRLVVA 
      STTHLPEEF RKKDLDFVR TVYGQKEAT PRWKECIWQ 
      ANRLHIAVS SIYVKKYVN KTTKYRVAE LTIDIKSSF 
      IETLKKIGW MDKKTKKHA LQKAEKMSS FIGYPDELL 
      DVNKVEDYY RGLEVDPKQ YSLRVSFNI SNFAHMKYV 
      QLLREPVLK PDWRDQAYS HFVNAFYDQ TTNSIELPA 
      AILQDSFFD PDRPQYMNY GAIGGVIGH EITHGFDDI 
      GRQYDKDGN VIDWWEPET NKTFASKSQ CLVEQYGNY 
      TIPEIEMSL NGVKNLGEN IADNGGIKI AYRGYMKWL 
      ERNKVELGL PGLPYSPRQ LFWISSAIN FCSKYKKER 
      LKQLVTLGH HAPNYFRVV VPFMNSDHF GRDFNCPLG 
      SPMNPKYKC RVW
    CDS sequence
    • >TC014700 RA
      atggacgaa tcgttgtct ggtaaaaag aaaagttcc 
      tggtgggaa aagcgcacc agatacgag aaatgccta 
      attgttcta accggattg tgtatcatc gtgataatt 
      tacctagct tcaaattta ctaatgcgg tttcctgcg 
      tccactgaa cacttgaaa aaagacgtc tgcatgacg 
      gaaaaatgc attcgttcg gcgtccagc attttggaa 
      aagatggac cgaactgtt gatccttgc gacgatttt 
      tacaaattc gcctgtggc aattttgtc gagaaacag 
      attattcct gaccacaaa acaatggtc tcgtctttc 
      ggcgaaacg agcgataag gtgagtctt caattgctc 
      aaagttttt gaagaggac atccatgag aaagaccct 
      cattctctg cgactcgtt aaatcaatt tacaaatcg 
      tgtatgaac accagtcag atcgaaaat gagggcctt 
      tctttcatg aagcgggaa tttcgggat ttgggcggc 
      tggccagtt atcgaaccc aactggaac gaaaaaacc 
      ttcgactgg aaaaatctt ttattccgt ctgaaacga 
      atcgggtgg cgacgtacg tttttcctg agcatgttc 
      attgacact gacttgaaa aactcgtcc aaaagaatt 
      ttcacaata agccaacct tgcatgccg tacgacaga 
      tacaagaaa ggctttaac gacacggct gttaaggcg 
      ggttatgag tacttggtc gaattggct gttcttttc 
      ggagctgac agaacggcc gcaagggcc gaaatgagg 
      gaagtggtc gagtttcaa atcgaactt gccaagatg 
      ataattccg tacgaagaa cggaaaaat gtctcgttg 
      agctacaat ccggttaca attcgcgaa ctccagaga 
      aatttcaca accattccg tggctcaaa ttgatcaat 
      aactactta tcgccaatt atgctcacg tctgacact 
      gttgtaaac gtggccgtg cccacattt ctggagcga 
      cttgagaac tttctgccc actgtgaag aaacgagtt 
      ttagccaat tacatgttc acgcgtctg gttgtggcc 
      tcaaccacg catttaccg gaagagttc cgaaagaag 
      gatttagac tttgtgaga actgtttac gggcagaag 
      gaagccaca cctcggtgg aaggaatgc atttggcag 
      gccaatcgt ttacacatt gctgtgtct tcaatttac 
      gtcaaaaaa tacgttaat aaaaccacc aagtaccga 
      gtggctgaa ctaacaatt gatatcaaa agttcgttc 
      atcgaaacg ttgaaaaaa atcggttgg atggacaag 
      aagacgaaa aaacacgcg ttacaaaag gccgagaaa 
      atgagctcg tttattgga tatccggac gagcttttg 
      gacgtcaat aaagtggag gattattat cgggggctg 
      gaggtggac cccaagcaa tactccctg agagtctct 
      ttcaacatt tcgaatttt gcccacatg aagtatgtt 
      caactgttg agagaaccg gtgttaaag cccgattgg 
      cgggaccag gcttattca cattttgtg aacgctttt 
      tacgaccaa accacaaac agtatcgaa ctgccggct 
      gcgatcctg caggactct ttcttcgac cccgacagg 
      ccgcagtac atgaattat ggagcaata ggcggagta 
      ataggccac gaaataact cacggcttc gatgatatc 
      ggccgacag tatgataaa gacggcaat gtgattgac 
      tggtgggag cccgaaacc aataaaact ttcgcctcg 
      aagagtcaa tgtctcgtc gaacaatac ggcaattac 
      accatcccc gaaatcgaa atgagctta aacggggtc 
      aaaaatttg ggcgagaat atcgccgat aacgggggg 
      attaagatc gcctacagg ggctacatg aagtggttg 
      gagaggaat aaagtcgag ttggggctg ccaggactg 
      ccgtacagc cccagacag ttgttctgg atctcgtcc 
      gccatcaat ttctgcagt aaatacaag aaggagaga 
      cttaaacaa ctcgtcact ttgggacat catgcgcct 
      aattatttc cgagttgtg gtgcctttt atgaattcc 
      gatcatttc gggagggac tttaattgc cctctaggc 
      tcgcccatg aaccccaaa tataagtgc agggtgtgg 
      tag
    mRNA sequence
    • >TC014700 RA
      aaaattatc caaaacccg taaagtggg gggacgtct 
      tttaagtca attattaac acaacttgc atttcacgt 
      tcgtagttt ggtggcaaa tgtgtaatt tttgaatta 
      aaatggacg aatcgttgt ctggtaaaa agaaaagtt 
      cctggtggg aaaagcgca ccagatacg agaaatgcc 
      taattgttc taaccggat tgtgtatca tcgtgataa 
      tttacctag cttcaaatt tactaatgc ggtttcctg 
      cgtccactg aacacttga aaaaagacg tctgcatga 
      cggaaaaat gcattcgtt cggcgtcca gcattttgg 
      aaaagatgg accgaactg ttgatcctt gcgacgatt 
      tttacaaat tcgcctgtg gcaattttg tcgagaaac 
      agattattc ctgaccaca aaacaatgg tctcgtctt 
      tcggcgaaa cgagcgata aggtgagtc ttcaattgc 
      tcaaagttt ttgaagagg acatccatg agaaagacc 
      ctcattctc tgcgactcg ttaaatcaa tttacaaat 
      cgtgtatga acaccagtc agatcgaaa atgagggcc 
      tttctttca tgaagcggg aatttcggg atttgggcg 
      gctggccag ttatcgaac ccaactgga acgaaaaaa 
      ccttcgact ggaaaaatc ttttattcc gtctgaaac 
      gaatcgggt ggcgacgta cgtttttcc tgagcatgt 
      tcattgaca ctgacttga aaaactcgt ccaaaagaa 
      ttttcacaa taagccaac cttgcatgc cgtacgaca 
      gatacaaga aaggcttta acgacacgg ctgttaagg 
      cgggttatg agtacttgg tcgaattgg ctgttcttt 
      tcggagctg acagaacgg ccgcaaggg ccgaaatga 
      gggaagtgg tcgagtttc aaatcgaac ttgccaaga 
      tgataattc cgtacgaag aacggaaaa atgtctcgt 
      tgagctaca atccggtta caattcgcg aactccaga 
      gaaatttca caaccattc cgtggctca aattgatca 
      ataactact tatcgccaa ttatgctca cgtctgaca 
      ctgttgtaa acgtggccg tgcccacat ttctggagc 
      gacttgaga actttctgc ccactgtga agaaacgag 
      ttttagcca attacatgt tcacgcgtc tggttgtgg 
      cctcaacca cgcatttac cggaagagt tccgaaaga 
      aggatttag actttgtga gaactgttt acgggcaga 
      aggaagcca cacctcggt ggaaggaat gcatttggc 
      aggccaatc gtttacaca ttgctgtgt cttcaattt 
      acgtcaaaa aatacgtta ataaaacca ccaagtacc 
      gagtggctg aactaacaa ttgatatca aaagttcgt 
      tcatcgaaa cgttgaaaa aaatcggtt ggatggaca 
      agaagacga aaaaacacg cgttacaaa aggccgaga 
      aaatgagct cgtttattg gatatccgg acgagcttt 
      tggacgtca ataaagtgg aggattatt atcgggggc 
      tggaggtgg accccaagc aatactccc tgagagtct 
      ctttcaaca tttcgaatt ttgcccaca tgaagtatg 
      ttcaactgt tgagagaac cggtgttaa agcccgatt 
      ggcgggacc aggcttatt cacattttg tgaacgctt 
      tttacgacc aaaccacaa acagtatcg aactgccgg 
      ctgcgatcc tgcaggact ctttcttcg accccgaca 
      ggccgcagt acatgaatt atggagcaa taggcggag 
      taataggcc acgaaataa ctcacggct tcgatgata 
      tcggccgac agtatgata aagacggca atgtgattg 
      actggtggg agcccgaaa ccaataaaa ctttcgcct 
      cgaagagtc aatgtctcg tcgaacaat acggcaatt 
      acaccatcc ccgaaatcg aaatgagct taaacgggg 
      tcaaaaatt tgggcgaga atatcgccg ataacgggg 
      ggattaaga tcgcctaca ggggctaca tgaagtggt 
      tggagagga ataaagtcg agttggggc tgccaggac 
      tgccgtaca gccccagac agttgttct ggatctcgt 
      ccgccatca atttctgca gtaaataca agaaggaga 
      gacttaaac aactcgtca ctttgggac atcatgcgc 
      ctaattatt tccgagttg tggtgcctt ttatgaatt 
      ccgatcatt tcgggaggg actttaatt gccctctag 
      gctcgccca tgaacccca aatataagt gcagggtgt 
      ggtagatac aaatttctg ttttcaaca tttaattat 
      taactgtaa taattataa gacctgttt tgtaagact 
      tgtaacctt aatataata aaatattac gataata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY