| General information | |||
| TC014672 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 13351382..13357569 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014672 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02321 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
- >TC014672 RA
MHQETLRLI QVLEKTVSP DKNELEQAS TFLEQAAAA NILEFIKTL SDILRHGGN SPVARMAAG LQLKNQLTS KDPNIKQLC QQRWLSAPE EIRNYVKKN VVGALGTET NRPSSAAQC VAYIAVTEL PHHQWPDLI VTLVNNVVQ PNSTEMQKE ATLETIGYI CQEIDSDVL VTQSNDILT AIIHGMRST EPSNHVRFA ATQALLNSL EFTKANFEK ATERNFIME VVCEATQSP DTQIKVAAL QCLVKIMSL YYQYMEPYM GQALFPITL EAMKSDNDA VALQGIEFW SNVSDEEVD LSIEANEAA DAGRPPARV SRHYAKGAL QFIVPILLQ KLTKQEELD DEDDWNPSK AAGVCLMLL ATCCENEVV PHVLPFIKE NIKSENWRF RDASLMAFG SILGGLDNT TLKPLVEQA MPTLIELMY DSSVIVRDT AAWTFGRIC EIIPEAAIN ENYLKPLLE SLINGLKAE PRVAANVCW AFSGLAEAA YDAAETNED TGTPDTYML SQYFEYIIQ RLLETTDRP DGAQANLRP AAYEALMEM VKNSPKDCY VTVQKTTMV ILERLQQVL QMETHITSH NDRSQFNDL QSLLCGTLQ SVLRKVTPE DAPQISDAI MTAMLTMFN SNSCKSGGV QEDALMAVS TLVEVLGEG FIKYMDAFK PFLYIGLKN HQEYQVCGT AVGLTGDIF RALKLKALP YCDEIMTLL LENLGDQSV HRSVKPQIL SVFGDIVLS IGPEFKKYL DVVLTTLAQ ASQAQVDRD DFDMVDYLN ELREGVLDA YTGIVQGLK GDGPTPNPD VLILEPHIP FIVQFITVV AQDTFNSDA TVAVAAGLV GDLCTAFGA PLLQLLDLE PINEMLAKG RRSGTNRTR TLATWATKE LRKLKANST AAVASCAFD GFRFSLACC GLTEGNLTK VWSTASILD SHYT
- >TC014672 RA
atgcatcaa gaaacactc cgacttata caagtgcta gaaaaaact gtttctccc gataaaaat gaactggag caagcatca acatttctt gagcaagct gccgccgct aatatctta gaatttatc aagactcta tccgatatc ttgcgacat gggggcaac agtcccgtt gctcgcatg gccgccggt ctccaactg aaaaaccaa ctcacgtcc aaagatccc aacatcaaa cagttatgt caacagaga tggctgtcg gcaccggag gagatacgc aactacgtc aagaaaaac gtcgtaggg gccctgggc acggagacc aaccggcct tcgtcagcg gcccaatgc gtggcttac atcgctgtc accgaatta ccgcaccac caatggccc gacctgatc gtcacgcta gtgaacaac gtagtccaa ccaaactcc accgaaatg cagaaagaa gcaacgctg gagaccatc ggctacatc tgccaggaa atcgactca gacgtgctc gttacccaa tccaacgac atcctcacg gcgatcatc cacgggatg cgctccaca gagcccagc aatcatgtc agatttgcg gcaacgcag gcgttgtta aattcgctc gaattcact aaagctaac ttcgaaaag gcaactgaa aggaatttc atcatggag gtggtgtgc gaagccact cagtcgcca gatacgcaa attaaagtt gcagcgtta caatgtttg gttaaaatt atgtctttg tattatcaa tatatggag ccctacatg ggccaagct ctctttccc attacttta gaagcgatg aaatctgac aatgatgcc gtggcgctt caaggtatt gaattttgg tcgaatgtt agcgacgaa gaagtggat ttgtccatc gaagccaat gaggcagcg gatgcaggc cggccccca gccagagta tcacgccac tacgccaaa ggtgcgctc caatttatc gtacccatc cttctgcaa aaactaacg aagcaagaa gagctcgat gacgaagac gactggaac ccgagcaaa gctgccggt gtttgttta atgcttttg gccacatgc tgcgaaaac gaagttgtt ccacacgtt ttacctttt atcaaagaa aacataaag tctgagaat tggcgattt agagatgcg tcgcttatg gccttcggg tcgattctt ggcgggtta gataacacc actcttaaa cccttggtg gagcaagcc atgccaact ttgattgag ctgatgtat gatagtagc gttatcgtg agagatact gcggcttgg actttcggc cgaatttgc gaaattatt cccgaagcg gccattaac gagaattat cttaagccc ttgctggaa agtttgatt aatggtctt aaggctgaa ccgcgagtt gcggccaat gtctgctgg gcgttttct ggattggct gaagccgcc tacgatgca gctgaaact aatgaagac acgggaaca cccgatacg tacatgttg tcacaatac tttgagtat ataattcag cggcttttg gaaactacg gaccgtcca gacggcgct caagctaat ctcagaccg gcagcctac gaggctttg atggaaatg gtgaagaat tcaccgaaa gattgctac gtcaccgta caaaagaca acaatggtg attcttgaa cgtttgcaa caagtttta caaatggaa acacacatt acgagccac aatgatagg tcacagttt aatgatctc cagtcgttg ttgtgcgga acacttcag tcggttttg aggaaagtt acaccagag gacgcgccg caaatatcc gacgccatc atgactgct atgctgacc atgttcaac tcgaattcg tgcaaaagt ggaggtgta caagaggac gcactgatg gcagtgtca accctggtg gaagtcctc ggtgaaggt ttcatcaaa tacatggac gcgttcaaa ccgtttctt tacatcggc cttaaaaac caccaagag taccaagtt tgtggtacc gcagtcggt ctaaccggt gacattttc cgcgctctc aaactaaaa gctcttcct tattgcgac gaaatcatg actcttctg ctcgaaaat ctcggcgat caatcggtc caccgtagc gtcaagcca cagattctt tcagtgttc ggcgatatc gtcctaagt atcggcccc gaattcaaa aaatacctc gatgttgtt ctcacaaca ctagcgcag gcgtcgcag gcacaagtc gatagagat gatttcgat atggtcgat tatttgaac gaattacgc gaaggtgta cttgatgct tacacgggc attgtacag ggtttaaaa ggcgatggc ccgacacct aatcccgac gttttgatt ctcgaaccg cacattcct ttcatcgtt caatttata accgtggtg gcacaagac acgttcaat tctgatgcc acggttgcg gtggctgcc ggattggtg ggggatctc tgtacggct tttggggca ccactttta caactcctg gatttggag ccgatcaac gagatgttg gcaaaaggc agacgaagt gggacaaat cgtactaga actttggcc acttgggct acgaaggag ttgcgcaag ttgaaggct aattcgacg gccgctgta gctagttgt gctttcgat ggtttcaga ttttctttg gcatgttgt ggtttaaca gaaggaaat ctaacgaag gtgtggtca acggcatcg atattggat tcgcattat acataa
- >TC014672 RA
tgcttcaag taaattgtt acaactgtg tgaaccgct gtttgatat aatttgggt aaacaacga ttgaaattg gccgtagtt ttgaaataa cgataagga accgtcatt catacgtta gcagcagcg tatgaatat agccttcct gccagttta atcatccta catgtccat gtggtgtta gccggctga ttgcaagaa ccaattaat taaaaccgt taagtttac aatattagt cagtaattc tacaataaa atgcatcaa gaaacactc cgacttata caagtgcta gaaaaaact gtttctccc gataaaaat gaactggag caagcatca acatttctt gagcaagct gccgccgct aatatctta gaatttatc aagactcta tccgatatc ttgcgacat gggggcaac agtcccgtt gctcgcatg gccgccggt ctccaactg aaaaaccaa ctcacgtcc aaagatccc aacatcaaa cagttatgt caacagaga tggctgtcg gcaccggag gagatacgc aactacgtc aagaaaaac gtcgtaggg gccctgggc acggagacc aaccggcct tcgtcagcg gcccaatgc gtggcttac atcgctgtc accgaatta ccgcaccac caatggccc gacctgatc gtcacgcta gtgaacaac gtagtccaa ccaaactcc accgaaatg cagaaagaa gcaacgctg gagaccatc ggctacatc tgccaggaa atcgactca gacgtgctc gttacccaa tccaacgac atcctcacg gcgatcatc cacgggatg cgctccaca gagcccagc aatcatgtc agatttgcg gcaacgcag gcgttgtta aattcgctc gaattcact aaagctaac ttcgaaaag gcaactgaa aggaatttc atcatggag gtggtgtgc gaagccact cagtcgcca gatacgcaa attaaagtt gcagcgtta caatgtttg gttaaaatt atgtctttg tattatcaa tatatggag ccctacatg ggccaagct ctctttccc attacttta gaagcgatg aaatctgac aatgatgcc gtggcgctt caaggtatt gaattttgg tcgaatgtt agcgacgaa gaagtggat ttgtccatc gaagccaat gaggcagcg gatgcaggc cggccccca gccagagta tcacgccac tacgccaaa ggtgcgctc caatttatc gtacccatc cttctgcaa aaactaacg aagcaagaa gagctcgat gacgaagac gactggaac ccgagcaaa gctgccggt gtttgttta atgcttttg gccacatgc tgcgaaaac gaagttgtt ccacacgtt ttacctttt atcaaagaa aacataaag tctgagaat tggcgattt agagatgcg tcgcttatg gccttcggg tcgattctt ggcgggtta gataacacc actcttaaa cccttggtg gagcaagcc atgccaact ttgattgag ctgatgtat gatagtagc gttatcgtg agagatact gcggcttgg actttcggc cgaatttgc gaaattatt cccgaagcg gccattaac gagaattat cttaagccc ttgctggaa agtttgatt aatggtctt aaggctgaa ccgcgagtt gcggccaat gtctgctgg gcgttttct ggattggct gaagccgcc tacgatgca gctgaaact aatgaagac acgggaaca cccgatacg tacatgttg tcacaatac tttgagtat ataattcag cggcttttg gaaactacg gaccgtcca gacggcgct caagctaat ctcagaccg gcagcctac gaggctttg atggaaatg gtgaagaat tcaccgaaa gattgctac gtcaccgta caaaagaca acaatggtg attcttgaa cgtttgcaa caagtttta caaatggaa acacacatt acgagccac aatgatagg tcacagttt aatgatctc cagtcgttg ttgtgcgga acacttcag tcggttttg aggaaagtt acaccagag gacgcgccg caaatatcc gacgccatc atgactgct atgctgacc atgttcaac tcgaattcg tgcaaaagt ggaggtgta caagaggac gcactgatg gcagtgtca accctggtg gaagtcctc ggtgaaggt ttcatcaaa tacatggac gcgttcaaa ccgtttctt tacatcggc cttaaaaac caccaagag taccaagtt tgtggtacc gcagtcggt ctaaccggt gacattttc cgcgctctc aaactaaaa gctcttcct tattgcgac gaaatcatg actcttctg ctcgaaaat ctcggcgat caatcggtc caccgtagc gtcaagcca cagattctt tcagtgttc ggcgatatc gtcctaagt atcggcccc gaattcaaa aaatacctc gatgttgtt ctcacaaca ctagcgcag gcgtcgcag gcacaagtc gatagagat gatttcgat atggtcgat tatttgaac gaattacgc gaaggtgta cttgatgct tacacgggc attgtacag ggtttaaaa ggcgatggc ccgacacct aatcccgac gttttgatt ctcgaaccg cacattcct ttcatcgtt caatttata accgtggtg gcacaagac acgttcaat tctgatgcc acggttgcg gtggctgcc ggattggtg ggggatctc tgtacggct tttggggca ccactttta caactcctg gatttggag ccgatcaac gagatgttg gcaaaaggc agacgaagt gggacaaat cgtactaga actttggcc acttgggct acgaaggag ttgcgcaag ttgaaggct aattcgacg gccgctgta gctagttgt gctttcgat ggtttcaga ttttctttg gcatgttgt ggtttaaca gaaggaaat ctaacgaag gtgtggtca acggcatcg atattggat tcgcattat acataaaac caaaattct tgcagactt tagttttcg ttttggctg agtcggacc agttttgct catctctcg gttgagtaa agctggtca gctagtttg acatggtat tgttcccca atactttcg gttttcggg gtgcagagt gagtgctga tgcatatgg actcttgat aaactgtga gtgctaatc gtagctgtg aacgcttgt gaatagtgt ctgaatctt cgcctggtt tcgtgaaag gcggtttag ctcaagact gaagagtga agtggtgtc tcctctatc acttgttgc gtcttttac gttcgtctt cactttagt gttgtactt gattacaat aaaagcatt tgtctattt cacatatcg aattaatca taagtagtt atttttata atggtcttt ggtgaagaa attacctat gcactgcag tcagacaga agtaatctt aaaatcctg atttattat tgcttcttt gtttcgctg aagttgtgt gtaatttca ttacggaaa aaagcaaaa cacgctctg gtgcttctt tagattttg cgaatttat attaacttt tctcttaaa atgcgaaag actgcgata atcaaaaac tgtccagag gagcgtgtt tcggttttt ccctggttg atttttttg aaacaattt ccaattcac tgaattcat actcaggaa atttgagaa taataaatc ttgtgactg gtatttttt atgaaagat tttgtgagg cctgattgt gccttgcgt gggtggtgt atgaaaatt ttcgagttt ttcgtataa gtcgacacg ctgattaaa tatacgaaa gtgcttgaa agggagggt gggtatcgc aacaaagta aaaaagaat ctgtgattt gagaaggtt tctgtagtt gccaaaaca cgaaaaaga tacattcaa attccatta gttttttaa atgtgtata acgaaacct tctcaatgt tttcgtaat tataattct agaattata attataaat tccttgtat gtgtgatat ttttccgtg ggcaatatt gaaataaac caatttttg tgtttattt ttatcacta gtccattaa gcgaccttc gttgttttg gaaggtaca ttagcgatg ggttatttt aactattgc ccacgtctg ggttcaaag gctgtatga aatcacttg tatagtatg taagtagtg tggttgtat attttgtat gtaagaagt atgaagaat tttattatg aagtagtgt aagaatttc tactggcct aaattattt attgaagct tctaactgt tttatgcac accgagata tttaatttt aaaaagctc atttttatg gttttgtga accagccaa agtgtgatt ttaatccat gatcttttt aaaattatt tcgaattct acgaatgtt catgtgcaa aaaggttgc gttacaatt aattggaag ggttttgag tggaaatgt ttatggttg catatttta ttggttgaa tgttttgtt ttgattgtg ccatgttga ttgttaatc tgtaatcac cccaaacgc gccgatgtt tgacaatat ggttttctg tgtgttctg aagactgaa tttattaat taaatagcc tagtaaagc tcaaatgcc gtatgaaag ttgacgcaa aatgcggct tattccaaa cttggtatt gtcgaacac cggttactt ttcttcttt tcccttaaa aatgttacc atgtctagg gtgcctatt gaccaattc atcaaaatt gtattagat tttaatttt gttaagtta aattccctg cacgccttt tcagttagg atatttcac tttttaaat tccctccca aacgcttgg gatcactcc cgcagaatt gggattttt gtagttgta cggataata aatgtttcc tttcttt
