Status
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Gene information
General information
TC014672 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13351382..13357569
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014672
    iBeetle screen iB_02321

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014672 RA
      MHQETLRLI QVLEKTVSP DKNELEQAS TFLEQAAAA 
      NILEFIKTL SDILRHGGN SPVARMAAG LQLKNQLTS 
      KDPNIKQLC QQRWLSAPE EIRNYVKKN VVGALGTET 
      NRPSSAAQC VAYIAVTEL PHHQWPDLI VTLVNNVVQ 
      PNSTEMQKE ATLETIGYI CQEIDSDVL VTQSNDILT 
      AIIHGMRST EPSNHVRFA ATQALLNSL EFTKANFEK 
      ATERNFIME VVCEATQSP DTQIKVAAL QCLVKIMSL 
      YYQYMEPYM GQALFPITL EAMKSDNDA VALQGIEFW 
      SNVSDEEVD LSIEANEAA DAGRPPARV SRHYAKGAL 
      QFIVPILLQ KLTKQEELD DEDDWNPSK AAGVCLMLL 
      ATCCENEVV PHVLPFIKE NIKSENWRF RDASLMAFG 
      SILGGLDNT TLKPLVEQA MPTLIELMY DSSVIVRDT 
      AAWTFGRIC EIIPEAAIN ENYLKPLLE SLINGLKAE 
      PRVAANVCW AFSGLAEAA YDAAETNED TGTPDTYML 
      SQYFEYIIQ RLLETTDRP DGAQANLRP AAYEALMEM 
      VKNSPKDCY VTVQKTTMV ILERLQQVL QMETHITSH 
      NDRSQFNDL QSLLCGTLQ SVLRKVTPE DAPQISDAI 
      MTAMLTMFN SNSCKSGGV QEDALMAVS TLVEVLGEG 
      FIKYMDAFK PFLYIGLKN HQEYQVCGT AVGLTGDIF 
      RALKLKALP YCDEIMTLL LENLGDQSV HRSVKPQIL 
      SVFGDIVLS IGPEFKKYL DVVLTTLAQ ASQAQVDRD 
      DFDMVDYLN ELREGVLDA YTGIVQGLK GDGPTPNPD 
      VLILEPHIP FIVQFITVV AQDTFNSDA TVAVAAGLV 
      GDLCTAFGA PLLQLLDLE PINEMLAKG RRSGTNRTR 
      TLATWATKE LRKLKANST AAVASCAFD GFRFSLACC 
      GLTEGNLTK VWSTASILD SHYT
    CDS sequence
    • >TC014672 RA
      atgcatcaa gaaacactc cgacttata caagtgcta 
      gaaaaaact gtttctccc gataaaaat gaactggag 
      caagcatca acatttctt gagcaagct gccgccgct 
      aatatctta gaatttatc aagactcta tccgatatc 
      ttgcgacat gggggcaac agtcccgtt gctcgcatg 
      gccgccggt ctccaactg aaaaaccaa ctcacgtcc 
      aaagatccc aacatcaaa cagttatgt caacagaga 
      tggctgtcg gcaccggag gagatacgc aactacgtc 
      aagaaaaac gtcgtaggg gccctgggc acggagacc 
      aaccggcct tcgtcagcg gcccaatgc gtggcttac 
      atcgctgtc accgaatta ccgcaccac caatggccc 
      gacctgatc gtcacgcta gtgaacaac gtagtccaa 
      ccaaactcc accgaaatg cagaaagaa gcaacgctg 
      gagaccatc ggctacatc tgccaggaa atcgactca 
      gacgtgctc gttacccaa tccaacgac atcctcacg 
      gcgatcatc cacgggatg cgctccaca gagcccagc 
      aatcatgtc agatttgcg gcaacgcag gcgttgtta 
      aattcgctc gaattcact aaagctaac ttcgaaaag 
      gcaactgaa aggaatttc atcatggag gtggtgtgc 
      gaagccact cagtcgcca gatacgcaa attaaagtt 
      gcagcgtta caatgtttg gttaaaatt atgtctttg 
      tattatcaa tatatggag ccctacatg ggccaagct 
      ctctttccc attacttta gaagcgatg aaatctgac 
      aatgatgcc gtggcgctt caaggtatt gaattttgg 
      tcgaatgtt agcgacgaa gaagtggat ttgtccatc 
      gaagccaat gaggcagcg gatgcaggc cggccccca 
      gccagagta tcacgccac tacgccaaa ggtgcgctc 
      caatttatc gtacccatc cttctgcaa aaactaacg 
      aagcaagaa gagctcgat gacgaagac gactggaac 
      ccgagcaaa gctgccggt gtttgttta atgcttttg 
      gccacatgc tgcgaaaac gaagttgtt ccacacgtt 
      ttacctttt atcaaagaa aacataaag tctgagaat 
      tggcgattt agagatgcg tcgcttatg gccttcggg 
      tcgattctt ggcgggtta gataacacc actcttaaa 
      cccttggtg gagcaagcc atgccaact ttgattgag 
      ctgatgtat gatagtagc gttatcgtg agagatact 
      gcggcttgg actttcggc cgaatttgc gaaattatt 
      cccgaagcg gccattaac gagaattat cttaagccc 
      ttgctggaa agtttgatt aatggtctt aaggctgaa 
      ccgcgagtt gcggccaat gtctgctgg gcgttttct 
      ggattggct gaagccgcc tacgatgca gctgaaact 
      aatgaagac acgggaaca cccgatacg tacatgttg 
      tcacaatac tttgagtat ataattcag cggcttttg 
      gaaactacg gaccgtcca gacggcgct caagctaat 
      ctcagaccg gcagcctac gaggctttg atggaaatg 
      gtgaagaat tcaccgaaa gattgctac gtcaccgta 
      caaaagaca acaatggtg attcttgaa cgtttgcaa 
      caagtttta caaatggaa acacacatt acgagccac 
      aatgatagg tcacagttt aatgatctc cagtcgttg 
      ttgtgcgga acacttcag tcggttttg aggaaagtt 
      acaccagag gacgcgccg caaatatcc gacgccatc 
      atgactgct atgctgacc atgttcaac tcgaattcg 
      tgcaaaagt ggaggtgta caagaggac gcactgatg 
      gcagtgtca accctggtg gaagtcctc ggtgaaggt 
      ttcatcaaa tacatggac gcgttcaaa ccgtttctt 
      tacatcggc cttaaaaac caccaagag taccaagtt 
      tgtggtacc gcagtcggt ctaaccggt gacattttc 
      cgcgctctc aaactaaaa gctcttcct tattgcgac 
      gaaatcatg actcttctg ctcgaaaat ctcggcgat 
      caatcggtc caccgtagc gtcaagcca cagattctt 
      tcagtgttc ggcgatatc gtcctaagt atcggcccc 
      gaattcaaa aaatacctc gatgttgtt ctcacaaca 
      ctagcgcag gcgtcgcag gcacaagtc gatagagat 
      gatttcgat atggtcgat tatttgaac gaattacgc 
      gaaggtgta cttgatgct tacacgggc attgtacag 
      ggtttaaaa ggcgatggc ccgacacct aatcccgac 
      gttttgatt ctcgaaccg cacattcct ttcatcgtt 
      caatttata accgtggtg gcacaagac acgttcaat 
      tctgatgcc acggttgcg gtggctgcc ggattggtg 
      ggggatctc tgtacggct tttggggca ccactttta 
      caactcctg gatttggag ccgatcaac gagatgttg 
      gcaaaaggc agacgaagt gggacaaat cgtactaga 
      actttggcc acttgggct acgaaggag ttgcgcaag 
      ttgaaggct aattcgacg gccgctgta gctagttgt 
      gctttcgat ggtttcaga ttttctttg gcatgttgt 
      ggtttaaca gaaggaaat ctaacgaag gtgtggtca 
      acggcatcg atattggat tcgcattat acataa
    mRNA sequence
    • >TC014672 RA
      tgcttcaag taaattgtt acaactgtg tgaaccgct 
      gtttgatat aatttgggt aaacaacga ttgaaattg 
      gccgtagtt ttgaaataa cgataagga accgtcatt 
      catacgtta gcagcagcg tatgaatat agccttcct 
      gccagttta atcatccta catgtccat gtggtgtta 
      gccggctga ttgcaagaa ccaattaat taaaaccgt 
      taagtttac aatattagt cagtaattc tacaataaa 
      atgcatcaa gaaacactc cgacttata caagtgcta 
      gaaaaaact gtttctccc gataaaaat gaactggag 
      caagcatca acatttctt gagcaagct gccgccgct 
      aatatctta gaatttatc aagactcta tccgatatc 
      ttgcgacat gggggcaac agtcccgtt gctcgcatg 
      gccgccggt ctccaactg aaaaaccaa ctcacgtcc 
      aaagatccc aacatcaaa cagttatgt caacagaga 
      tggctgtcg gcaccggag gagatacgc aactacgtc 
      aagaaaaac gtcgtaggg gccctgggc acggagacc 
      aaccggcct tcgtcagcg gcccaatgc gtggcttac 
      atcgctgtc accgaatta ccgcaccac caatggccc 
      gacctgatc gtcacgcta gtgaacaac gtagtccaa 
      ccaaactcc accgaaatg cagaaagaa gcaacgctg 
      gagaccatc ggctacatc tgccaggaa atcgactca 
      gacgtgctc gttacccaa tccaacgac atcctcacg 
      gcgatcatc cacgggatg cgctccaca gagcccagc 
      aatcatgtc agatttgcg gcaacgcag gcgttgtta 
      aattcgctc gaattcact aaagctaac ttcgaaaag 
      gcaactgaa aggaatttc atcatggag gtggtgtgc 
      gaagccact cagtcgcca gatacgcaa attaaagtt 
      gcagcgtta caatgtttg gttaaaatt atgtctttg 
      tattatcaa tatatggag ccctacatg ggccaagct 
      ctctttccc attacttta gaagcgatg aaatctgac 
      aatgatgcc gtggcgctt caaggtatt gaattttgg 
      tcgaatgtt agcgacgaa gaagtggat ttgtccatc 
      gaagccaat gaggcagcg gatgcaggc cggccccca 
      gccagagta tcacgccac tacgccaaa ggtgcgctc 
      caatttatc gtacccatc cttctgcaa aaactaacg 
      aagcaagaa gagctcgat gacgaagac gactggaac 
      ccgagcaaa gctgccggt gtttgttta atgcttttg 
      gccacatgc tgcgaaaac gaagttgtt ccacacgtt 
      ttacctttt atcaaagaa aacataaag tctgagaat 
      tggcgattt agagatgcg tcgcttatg gccttcggg 
      tcgattctt ggcgggtta gataacacc actcttaaa 
      cccttggtg gagcaagcc atgccaact ttgattgag 
      ctgatgtat gatagtagc gttatcgtg agagatact 
      gcggcttgg actttcggc cgaatttgc gaaattatt 
      cccgaagcg gccattaac gagaattat cttaagccc 
      ttgctggaa agtttgatt aatggtctt aaggctgaa 
      ccgcgagtt gcggccaat gtctgctgg gcgttttct 
      ggattggct gaagccgcc tacgatgca gctgaaact 
      aatgaagac acgggaaca cccgatacg tacatgttg 
      tcacaatac tttgagtat ataattcag cggcttttg 
      gaaactacg gaccgtcca gacggcgct caagctaat 
      ctcagaccg gcagcctac gaggctttg atggaaatg 
      gtgaagaat tcaccgaaa gattgctac gtcaccgta 
      caaaagaca acaatggtg attcttgaa cgtttgcaa 
      caagtttta caaatggaa acacacatt acgagccac 
      aatgatagg tcacagttt aatgatctc cagtcgttg 
      ttgtgcgga acacttcag tcggttttg aggaaagtt 
      acaccagag gacgcgccg caaatatcc gacgccatc 
      atgactgct atgctgacc atgttcaac tcgaattcg 
      tgcaaaagt ggaggtgta caagaggac gcactgatg 
      gcagtgtca accctggtg gaagtcctc ggtgaaggt 
      ttcatcaaa tacatggac gcgttcaaa ccgtttctt 
      tacatcggc cttaaaaac caccaagag taccaagtt 
      tgtggtacc gcagtcggt ctaaccggt gacattttc 
      cgcgctctc aaactaaaa gctcttcct tattgcgac 
      gaaatcatg actcttctg ctcgaaaat ctcggcgat 
      caatcggtc caccgtagc gtcaagcca cagattctt 
      tcagtgttc ggcgatatc gtcctaagt atcggcccc 
      gaattcaaa aaatacctc gatgttgtt ctcacaaca 
      ctagcgcag gcgtcgcag gcacaagtc gatagagat 
      gatttcgat atggtcgat tatttgaac gaattacgc 
      gaaggtgta cttgatgct tacacgggc attgtacag 
      ggtttaaaa ggcgatggc ccgacacct aatcccgac 
      gttttgatt ctcgaaccg cacattcct ttcatcgtt 
      caatttata accgtggtg gcacaagac acgttcaat 
      tctgatgcc acggttgcg gtggctgcc ggattggtg 
      ggggatctc tgtacggct tttggggca ccactttta 
      caactcctg gatttggag ccgatcaac gagatgttg 
      gcaaaaggc agacgaagt gggacaaat cgtactaga 
      actttggcc acttgggct acgaaggag ttgcgcaag 
      ttgaaggct aattcgacg gccgctgta gctagttgt 
      gctttcgat ggtttcaga ttttctttg gcatgttgt 
      ggtttaaca gaaggaaat ctaacgaag gtgtggtca 
      acggcatcg atattggat tcgcattat acataaaac 
      caaaattct tgcagactt tagttttcg ttttggctg 
      agtcggacc agttttgct catctctcg gttgagtaa 
      agctggtca gctagtttg acatggtat tgttcccca 
      atactttcg gttttcggg gtgcagagt gagtgctga 
      tgcatatgg actcttgat aaactgtga gtgctaatc 
      gtagctgtg aacgcttgt gaatagtgt ctgaatctt 
      cgcctggtt tcgtgaaag gcggtttag ctcaagact 
      gaagagtga agtggtgtc tcctctatc acttgttgc 
      gtcttttac gttcgtctt cactttagt gttgtactt 
      gattacaat aaaagcatt tgtctattt cacatatcg 
      aattaatca taagtagtt atttttata atggtcttt 
      ggtgaagaa attacctat gcactgcag tcagacaga 
      agtaatctt aaaatcctg atttattat tgcttcttt 
      gtttcgctg aagttgtgt gtaatttca ttacggaaa 
      aaagcaaaa cacgctctg gtgcttctt tagattttg 
      cgaatttat attaacttt tctcttaaa atgcgaaag 
      actgcgata atcaaaaac tgtccagag gagcgtgtt 
      tcggttttt ccctggttg atttttttg aaacaattt 
      ccaattcac tgaattcat actcaggaa atttgagaa 
      taataaatc ttgtgactg gtatttttt atgaaagat 
      tttgtgagg cctgattgt gccttgcgt gggtggtgt 
      atgaaaatt ttcgagttt ttcgtataa gtcgacacg 
      ctgattaaa tatacgaaa gtgcttgaa agggagggt 
      gggtatcgc aacaaagta aaaaagaat ctgtgattt 
      gagaaggtt tctgtagtt gccaaaaca cgaaaaaga 
      tacattcaa attccatta gttttttaa atgtgtata 
      acgaaacct tctcaatgt tttcgtaat tataattct 
      agaattata attataaat tccttgtat gtgtgatat 
      ttttccgtg ggcaatatt gaaataaac caatttttg 
      tgtttattt ttatcacta gtccattaa gcgaccttc 
      gttgttttg gaaggtaca ttagcgatg ggttatttt 
      aactattgc ccacgtctg ggttcaaag gctgtatga 
      aatcacttg tatagtatg taagtagtg tggttgtat 
      attttgtat gtaagaagt atgaagaat tttattatg 
      aagtagtgt aagaatttc tactggcct aaattattt 
      attgaagct tctaactgt tttatgcac accgagata 
      tttaatttt aaaaagctc atttttatg gttttgtga 
      accagccaa agtgtgatt ttaatccat gatcttttt 
      aaaattatt tcgaattct acgaatgtt catgtgcaa 
      aaaggttgc gttacaatt aattggaag ggttttgag 
      tggaaatgt ttatggttg catatttta ttggttgaa 
      tgttttgtt ttgattgtg ccatgttga ttgttaatc 
      tgtaatcac cccaaacgc gccgatgtt tgacaatat 
      ggttttctg tgtgttctg aagactgaa tttattaat 
      taaatagcc tagtaaagc tcaaatgcc gtatgaaag 
      ttgacgcaa aatgcggct tattccaaa cttggtatt 
      gtcgaacac cggttactt ttcttcttt tcccttaaa 
      aatgttacc atgtctagg gtgcctatt gaccaattc 
      atcaaaatt gtattagat tttaatttt gttaagtta 
      aattccctg cacgccttt tcagttagg atatttcac 
      tttttaaat tccctccca aacgcttgg gatcactcc 
      cgcagaatt gggattttt gtagttgta cggataata 
      aatgtttcc tttcttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY