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Gene information
General information
TC014663 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13152466..13155000
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014663
    iBeetle screen iB_02320

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014663 RA
      MAGLPKLPG LTFTDPTLT SYHLSQTFS ISNGYKIPK 
NDYVGIGNR ELDSNSVKF ISTNDPVRF DPSLTYGRT 
KAIPVQMFK PHYALYDQK CLTFKAFFK QGVAESPDE 
YYRVRHVNI IYFLEDDTI TIMEPRTPN SGIDQGRLL 
KRHRIPKSE DGEFWHWKD FGVGRNIVF YGVVYHLVD 
CDTFTREYM ASQGLVMAE PEQLPPDPY TQNRLMKSH 
QNNFTKTPS ADDKFRRFL EYDGKILKF KAIWDDRDS 
EYGEIRKYE VLYFLADDT VAVKEIHTK NDGRDPYSQ 
LLRKTKLPK IWTDRPAHF PSIYLETTD EEVTEFYQP 
KDLRVGETI FVLGRKMLL YDCDDFTRN YFKKALCME 
QGPPMDISE KPKPPPEKK IPPHDGIGS LEDSLQNTL 
TFMPKPPRK DVVKQILNA NKYLRYEMK MDDIHPEDS 
IRRFLLLYS LGDNTCKIF EPPIENSGI IGGKYLRST 
LLPKPNTNP LDPEYYSPI DFYIGATIT VFQQRFIIT 
GADLYVYRY MQANSSKFP CEVIENIRN YMFNKGYLK 
DDIEKQVEE NKIDIEKAE RDAIGKNLI YTQTEMEKC 
LERFNIKDE REDPTFQKE YVKEPLVIP EHNVPSFGI 
QPVNNECPY PITTPPIPS LDCKSEAQR IASTEYDTE 
EEKIKKYYD KVLREHKHI CENAEPQQP PPEDDCLAK 
ERKGKSVKF DDNVKCE
    CDS sequence
    • >TC014663 RA
      atggcaggt ttgccgaaa ttgccgggg ctcaccttc 
actgacccc actctgacc agttaccac ctatcccag 
acctttagc atttcaaat gggtataaa atcccaaag 
aacgactac gtggggata ggaaatcgg gaactggac 
tcaaattcg gtcaaattc attagtacc aacgacccg 
gtccgcttc gatccctcc ctgacttat ggacgaacc 
aaagcaatt cctgtccaa atgttcaaa ccgcattac 
gccctttat gaccaaaag tgtctcact tttaaggct 
tttttcaag cagggggtt gcagagtcg cccgatgag 
tactacaga gtgaggcat gtgaacata atctacttt 
ctggaagac gatacgatc acgattatg gaaccccga 
accccaaat agtgggatt gatcaaggc aggcttttg 
aaacgacac cgaattccc aaaagcgaa gacggggaa 
ttctggcat tggaaggac tttggtgtg ggcagaaat 
attgttttc tacggagtt gtctatcat ttggtggat 
tgcgatact tttacaagg gaatatatg gccagtcag 
ggattggta atggctgaa cctgagcaa ttaccgcct 
gatccctac acccaaaat agattaatg aagtcgcat 
cagaataat tttactaaa acgccttcg gcggatgat 
aaatttaga cgctttctg gaatatgac ggcaaaatt 
ctcaaattt aaagcgatt tgggacgac agagactcg 
gaatatggt gaaataagg aaatacgaa gttttatat 
tttctagca gatgacact gttgccgtc aaagaaatt 
cacaccaaa aatgatggc cgggatcct tactcccaa 
cttttacgc aaaactaaa ttaccaaag atttggacc 
gacagaccg gcccacttc ccctcaatc tatctggag 
acaaccgac gaagaggtc acagagttc taccagcct 
aaagactta cgcgtcgga gaaacaatt ttcgttcta 
gggcggaaa atgctcctt tacgactgt gatgacttt 
acgcgtaac tactttaag aaagcgctc tgcatggag 
cagggcccc cctatggac atttcagag aagcctaaa 
ccacccccc gagaagaaa attccccct catgacggc 
atcggtagc ttggaggat tccctccaa aacacccta 
acttttatg ccaaaaccg ccgcgaaaa gacgtggtt 
aagcaaatt ctgaacgca aataaatat ctcagatac 
gagatgaag atggatgat atacatcca gaagattca 
attcgtagg tttttgttg ctgtattct ttgggtgac 
aacacttgt aaaattttc gagcccccg attgaaaat 
tcggggatt attgggggg aagtatttg aggagtact 
ttgttgcca aaacctaac actaatcca cttgatccc 
gaatattac tcgccgatt gatttttat attggggct 
acgataact gtctttcag cagaggttt ataatcacg 
ggggctgac ttgtatgtg tatcgctac atgcaagcc 
aactcgagt aaatttccg tgcgaagtc attgaaaat 
attcggaac tatatgttc aataaaggg tacctcaag 
gatgatatt gagaagcaa gttgaggag aataaaatt 
gatattgaa aaggctgaa cgagacgca atagggaaa 
aacctgatc tacactcag accgaaatg gaaaagtgt 
ttggaacgt tttaatatt aaagacgaa agagaagac 
ccaactttc cagaaggaa tacgtgaaa gaaccattg 
gtgattcca gaacacaat gttccatca ttcgggata 
caacctgtt aataacgaa tgcccctac cctataaca 
acaccgccg attccgagc cttgattgc aaaagtgaa 
gcgcaacgg attgcatcc actgagtac gacacggag 
gaggaaaaa atcaagaaa tattacgac aaagtattg 
cgcgaacac aaacacatt tgtgagaat gctgaacca 
caacagccg ccaccagaa gacgactgt ctggcaaaa 
gaacgcaaa gggaaaagt gtaaaattt gatgataac 
gtaaaatgt gaataa
    mRNA sequence
    • >TC014663 RA
      tgaaaatgg caggtttgc cgaaattgc cggggctca 
ccttcactg accccactc tgaccagtt accacctat 
cccagacct ttagcattt caaatgggt ataaaatcc 
caaagaacg actacgtgg ggataggaa atcgggaac 
tggactcaa attcggtca aattcatta gtaccaacg 
acccggtcc gcttcgatc cctccctga cttatggac 
gaaccaaag caattcctg tccaaatgt tcaaaccgc 
attacgccc tttatgacc aaaagtgtc tcactttta 
aggcttttt tcaagcagg gggttgcag agtcgcccg 
atgagtact acagagtga ggcatgtga acataatct 
actttctgg aagacgata cgatcacga ttatggaac 
cccgaaccc caaatagtg ggattgatc aaggcaggc 
ttttgaaac gacaccgaa ttcccaaaa gcgaagacg 
gggaattct ggcattgga aggactttg gtgtgggca 
gaaatattg ttttctacg gagttgtct atcatttgg 
tggattgcg atactttta caagggaat atatggcca 
gtcagggat tggtaatgg ctgaacctg agcaattac 
cgcctgatc cctacaccc aaaatagat taatgaagt 
cgcatcaga ataatttta ctaaaacgc cttcggcgg 
atgataaat ttagacgct ttctggaat atgacggca 
aaattctca aatttaaag cgatttggg acgacagag 
actcggaat atggtgaaa taaggaaat acgaagttt 
tatattttc tagcagatg acactgttg ccgtcaaag 
aaattcaca ccaaaaatg atggccggg atccttact 
cccaacttt tacgcaaaa ctaaattac caaagattt 
ggaccgaca gaccggccc acttcccct caatctatc 
tggagacaa ccgacgaag aggtcacag agttctacc 
agcctaaag acttacgcg tcggagaaa caattttcg 
ttctagggc ggaaaatgc tcctttacg actgtgatg 
actttacgc gtaactact ttaagaaag cgctctgca 
tggagcagg gccccccta tggacattt cagagaagc 
ctaaaccac cccccgaga agaaaattc cccctcatg 
acggcatcg gtagcttgg aggattccc tccaaaaca 
ccctaactt ttatgccaa aaccgccgc gaaaagacg 
tggttaagc aaattctga acgcaaata aatatctca 
gatacgaga tgaagatgg atgatatac atccagaag 
attcaattc gtaggtttt tgttgctgt attctttgg 
gtgacaaca cttgtaaaa ttttcgagc ccccgattg 
aaaattcgg ggattattg gggggaagt atttgagga 
gtactttgt tgccaaaac ctaacacta atccacttg 
atcccgaat attactcgc cgattgatt tttatattg 
gggctacga taactgtct ttcagcaga ggtttataa 
tcacggggg ctgacttgt atgtgtatc gctacatgc 
aagccaact cgagtaaat ttccgtgcg aagtcattg 
aaaatattc ggaactata tgttcaata aagggtacc 
tcaaggatg atattgaga agcaagttg aggagaata 
aaattgata ttgaaaagg ctgaacgag acgcaatag 
ggaaaaacc tgatctaca ctcagaccg aaatggaaa 
agtgtttgg aacgtttta atattaaag acgaaagag 
aagacccaa ctttccaga aggaatacg tgaaagaac 
cattggtga ttccagaac acaatgttc catcattcg 
ggatacaac ctgttaata acgaatgcc cctacccta 
taacaacac cgccgattc cgagccttg attgcaaaa 
gtgaagcgc aacggattg catccactg agtacgaca 
cggaggagg aaaaaatca agaaatatt acgacaaag 
tattgcgcg aacacaaac acatttgtg agaatgctg 
aaccacaac agccgccac cagaagacg actgtctgg 
caaaagaac gcaaaggga aaagtgtaa aatttgatg 
ataacgtaa aatgtgaat aataaattt ctgtaaata 
tatttcgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY