| General information | |||
| TC014663 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 13152466..13155000 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014663 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02320 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
- >TC014663 RA
MAGLPKLPG LTFTDPTLT SYHLSQTFS ISNGYKIPK NDYVGIGNR ELDSNSVKF ISTNDPVRF DPSLTYGRT KAIPVQMFK PHYALYDQK CLTFKAFFK QGVAESPDE YYRVRHVNI IYFLEDDTI TIMEPRTPN SGIDQGRLL KRHRIPKSE DGEFWHWKD FGVGRNIVF YGVVYHLVD CDTFTREYM ASQGLVMAE PEQLPPDPY TQNRLMKSH QNNFTKTPS ADDKFRRFL EYDGKILKF KAIWDDRDS EYGEIRKYE VLYFLADDT VAVKEIHTK NDGRDPYSQ LLRKTKLPK IWTDRPAHF PSIYLETTD EEVTEFYQP KDLRVGETI FVLGRKMLL YDCDDFTRN YFKKALCME QGPPMDISE KPKPPPEKK IPPHDGIGS LEDSLQNTL TFMPKPPRK DVVKQILNA NKYLRYEMK MDDIHPEDS IRRFLLLYS LGDNTCKIF EPPIENSGI IGGKYLRST LLPKPNTNP LDPEYYSPI DFYIGATIT VFQQRFIIT GADLYVYRY MQANSSKFP CEVIENIRN YMFNKGYLK DDIEKQVEE NKIDIEKAE RDAIGKNLI YTQTEMEKC LERFNIKDE REDPTFQKE YVKEPLVIP EHNVPSFGI QPVNNECPY PITTPPIPS LDCKSEAQR IASTEYDTE EEKIKKYYD KVLREHKHI CENAEPQQP PPEDDCLAK ERKGKSVKF DDNVKCE
- >TC014663 RA
atggcaggt ttgccgaaa ttgccgggg ctcaccttc actgacccc actctgacc agttaccac ctatcccag acctttagc atttcaaat gggtataaa atcccaaag aacgactac gtggggata ggaaatcgg gaactggac tcaaattcg gtcaaattc attagtacc aacgacccg gtccgcttc gatccctcc ctgacttat ggacgaacc aaagcaatt cctgtccaa atgttcaaa ccgcattac gccctttat gaccaaaag tgtctcact tttaaggct tttttcaag cagggggtt gcagagtcg cccgatgag tactacaga gtgaggcat gtgaacata atctacttt ctggaagac gatacgatc acgattatg gaaccccga accccaaat agtgggatt gatcaaggc aggcttttg aaacgacac cgaattccc aaaagcgaa gacggggaa ttctggcat tggaaggac tttggtgtg ggcagaaat attgttttc tacggagtt gtctatcat ttggtggat tgcgatact tttacaagg gaatatatg gccagtcag ggattggta atggctgaa cctgagcaa ttaccgcct gatccctac acccaaaat agattaatg aagtcgcat cagaataat tttactaaa acgccttcg gcggatgat aaatttaga cgctttctg gaatatgac ggcaaaatt ctcaaattt aaagcgatt tgggacgac agagactcg gaatatggt gaaataagg aaatacgaa gttttatat tttctagca gatgacact gttgccgtc aaagaaatt cacaccaaa aatgatggc cgggatcct tactcccaa cttttacgc aaaactaaa ttaccaaag atttggacc gacagaccg gcccacttc ccctcaatc tatctggag acaaccgac gaagaggtc acagagttc taccagcct aaagactta cgcgtcgga gaaacaatt ttcgttcta gggcggaaa atgctcctt tacgactgt gatgacttt acgcgtaac tactttaag aaagcgctc tgcatggag cagggcccc cctatggac atttcagag aagcctaaa ccacccccc gagaagaaa attccccct catgacggc atcggtagc ttggaggat tccctccaa aacacccta acttttatg ccaaaaccg ccgcgaaaa gacgtggtt aagcaaatt ctgaacgca aataaatat ctcagatac gagatgaag atggatgat atacatcca gaagattca attcgtagg tttttgttg ctgtattct ttgggtgac aacacttgt aaaattttc gagcccccg attgaaaat tcggggatt attgggggg aagtatttg aggagtact ttgttgcca aaacctaac actaatcca cttgatccc gaatattac tcgccgatt gatttttat attggggct acgataact gtctttcag cagaggttt ataatcacg ggggctgac ttgtatgtg tatcgctac atgcaagcc aactcgagt aaatttccg tgcgaagtc attgaaaat attcggaac tatatgttc aataaaggg tacctcaag gatgatatt gagaagcaa gttgaggag aataaaatt gatattgaa aaggctgaa cgagacgca atagggaaa aacctgatc tacactcag accgaaatg gaaaagtgt ttggaacgt tttaatatt aaagacgaa agagaagac ccaactttc cagaaggaa tacgtgaaa gaaccattg gtgattcca gaacacaat gttccatca ttcgggata caacctgtt aataacgaa tgcccctac cctataaca acaccgccg attccgagc cttgattgc aaaagtgaa gcgcaacgg attgcatcc actgagtac gacacggag gaggaaaaa atcaagaaa tattacgac aaagtattg cgcgaacac aaacacatt tgtgagaat gctgaacca caacagccg ccaccagaa gacgactgt ctggcaaaa gaacgcaaa gggaaaagt gtaaaattt gatgataac gtaaaatgt gaataa
- >TC014663 RA
tgaaaatgg caggtttgc cgaaattgc cggggctca ccttcactg accccactc tgaccagtt accacctat cccagacct ttagcattt caaatgggt ataaaatcc caaagaacg actacgtgg ggataggaa atcgggaac tggactcaa attcggtca aattcatta gtaccaacg acccggtcc gcttcgatc cctccctga cttatggac gaaccaaag caattcctg tccaaatgt tcaaaccgc attacgccc tttatgacc aaaagtgtc tcactttta aggcttttt tcaagcagg gggttgcag agtcgcccg atgagtact acagagtga ggcatgtga acataatct actttctgg aagacgata cgatcacga ttatggaac cccgaaccc caaatagtg ggattgatc aaggcaggc ttttgaaac gacaccgaa ttcccaaaa gcgaagacg gggaattct ggcattgga aggactttg gtgtgggca gaaatattg ttttctacg gagttgtct atcatttgg tggattgcg atactttta caagggaat atatggcca gtcagggat tggtaatgg ctgaacctg agcaattac cgcctgatc cctacaccc aaaatagat taatgaagt cgcatcaga ataatttta ctaaaacgc cttcggcgg atgataaat ttagacgct ttctggaat atgacggca aaattctca aatttaaag cgatttggg acgacagag actcggaat atggtgaaa taaggaaat acgaagttt tatattttc tagcagatg acactgttg ccgtcaaag aaattcaca ccaaaaatg atggccggg atccttact cccaacttt tacgcaaaa ctaaattac caaagattt ggaccgaca gaccggccc acttcccct caatctatc tggagacaa ccgacgaag aggtcacag agttctacc agcctaaag acttacgcg tcggagaaa caattttcg ttctagggc ggaaaatgc tcctttacg actgtgatg actttacgc gtaactact ttaagaaag cgctctgca tggagcagg gccccccta tggacattt cagagaagc ctaaaccac cccccgaga agaaaattc cccctcatg acggcatcg gtagcttgg aggattccc tccaaaaca ccctaactt ttatgccaa aaccgccgc gaaaagacg tggttaagc aaattctga acgcaaata aatatctca gatacgaga tgaagatgg atgatatac atccagaag attcaattc gtaggtttt tgttgctgt attctttgg gtgacaaca cttgtaaaa ttttcgagc ccccgattg aaaattcgg ggattattg gggggaagt atttgagga gtactttgt tgccaaaac ctaacacta atccacttg atcccgaat attactcgc cgattgatt tttatattg gggctacga taactgtct ttcagcaga ggtttataa tcacggggg ctgacttgt atgtgtatc gctacatgc aagccaact cgagtaaat ttccgtgcg aagtcattg aaaatattc ggaactata tgttcaata aagggtacc tcaaggatg atattgaga agcaagttg aggagaata aaattgata ttgaaaagg ctgaacgag acgcaatag ggaaaaacc tgatctaca ctcagaccg aaatggaaa agtgtttgg aacgtttta atattaaag acgaaagag aagacccaa ctttccaga aggaatacg tgaaagaac cattggtga ttccagaac acaatgttc catcattcg ggatacaac ctgttaata acgaatgcc cctacccta taacaacac cgccgattc cgagccttg attgcaaaa gtgaagcgc aacggattg catccactg agtacgaca cggaggagg aaaaaatca agaaatatt acgacaaag tattgcgcg aacacaaac acatttgtg agaatgctg aaccacaac agccgccac cagaagacg actgtctgg caaaagaac gcaaaggga aaagtgtaa aatttgatg ataacgtaa aatgtgaat aataaattt ctgtaaata tatttcgc
