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Gene information
General information
TC014663 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 5237052..5239586
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014663
    iBeetle screen iB_02320
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014663 RA
      MAGLPKLPG LTFTDPTLT SYHLSQTFS ISNGYKIPK 
      NDYVGIGNR ELDSNSVKF ISTNDPVRF DPSLTYGRT 
      KAIPVQMFK PHYALYDQK CLTFKAFFK QGVAESPDE 
      YYRVRHVNI IYFLEDDTI TIMEPRTPN SGIDQGRLL 
      KRHRIPKSE DGEFWHWKD FGVGRNIVF YGVVYHLVD 
      CDTFTREYM ASQGLVMAE PEQLPPDPY TQNRLMKSH 
      QNNFTKTPS ADDKFRRFL EYDGKILKF KAIWDDRDS 
      EYGEIRKYE VLYFLADDT VAVKEIHTK NDGRDPYSQ 
      LLRKTKLPK IWTDRPAHF PSIYLETTD EEVTEFYQP 
      KDLRVGETI FVLGRKMLL YDCDDFTRN YFKKALCME 
      QGPPMDISE KPKPPPEKK IPPHDGIGS LEDSLQNTL 
      TFMPKPPRK DVVKQILNA NKYLRYEMK MDDIHPEDS 
      IRRFLLLYS LGDNTCKIF EPPIENSGI IGGKYLRST 
      LLPKPNTNP LDPEYYSPI DFYIGATIT VFQQRFIIT 
      GADLYVYRY MQANSSKFP CEVIENIRN YMFNKGYLK 
      DDIEKQVEE NKIDIEKAE RDAIGKNLI YTQTEMEKC 
      LERFNIKDE REDPTFQKE YVKEPLVIP EHNVPSFGI 
      QPVNNECPY PITTPPIPS LDCKSEAQR IASTEYDTE 
      EEKIKKYYD KVLREHKHI CENAEPQQP PPEDDCLAK 
      ERKGKSVKF DDNVKCE
    CDS sequence
    • >TC014663 RA
      atggcaggt ttgccgaaa ttgccgggg ctcaccttc 
      actgacccc actctgacc agttaccac ctatcccag 
      acctttagc atttcaaat gggtataaa atcccaaag 
      aacgactac gtggggata ggaaatcgg gaactggac 
      tcaaattcg gtcaaattc attagtacc aacgacccg 
      gtccgcttc gatccctcc ctgacttat ggacgaacc 
      aaagcaatt cctgtccaa atgttcaaa ccgcattac 
      gccctttat gaccaaaag tgtctcact tttaaggct 
      tttttcaag cagggggtt gcagagtcg cccgatgag 
      tactacaga gtgaggcat gtgaacata atctacttt 
      ctggaagac gatacgatc acgattatg gaaccccga 
      accccaaat agtgggatt gatcaaggc aggcttttg 
      aaacgacac cgaattccc aaaagcgaa gacggggaa 
      ttctggcat tggaaggac tttggtgtg ggcagaaat 
      attgttttc tacggagtt gtctatcat ttggtggat 
      tgcgatact tttacaagg gaatatatg gccagtcag 
      ggattggta atggctgaa cctgagcaa ttaccgcct 
      gatccctac acccaaaat agattaatg aagtcgcat 
      cagaataat tttactaaa acgccttcg gcggatgat 
      aaatttaga cgctttctg gaatatgac ggcaaaatt 
      ctcaaattt aaagcgatt tgggacgac agagactcg 
      gaatatggt gaaataagg aaatacgaa gttttatat 
      tttctagca gatgacact gttgccgtc aaagaaatt 
      cacaccaaa aatgatggc cgggatcct tactcccaa 
      cttttacgc aaaactaaa ttaccaaag atttggacc 
      gacagaccg gcccacttc ccctcaatc tatctggag 
      acaaccgac gaagaggtc acagagttc taccagcct 
      aaagactta cgcgtcgga gaaacaatt ttcgttcta 
      gggcggaaa atgctcctt tacgactgt gatgacttt 
      acgcgtaac tactttaag aaagcgctc tgcatggag 
      cagggcccc cctatggac atttcagag aagcctaaa 
      ccacccccc gagaagaaa attccccct catgacggc 
      atcggtagc ttggaggat tccctccaa aacacccta 
      acttttatg ccaaaaccg ccgcgaaaa gacgtggtt 
      aagcaaatt ctgaacgca aataaatat ctcagatac 
      gagatgaag atggatgat atacatcca gaagattca 
      attcgtagg tttttgttg ctgtattct ttgggtgac 
      aacacttgt aaaattttc gagcccccg attgaaaat 
      tcggggatt attgggggg aagtatttg aggagtact 
      ttgttgcca aaacctaac actaatcca cttgatccc 
      gaatattac tcgccgatt gatttttat attggggct 
      acgataact gtctttcag cagaggttt ataatcacg 
      ggggctgac ttgtatgtg tatcgctac atgcaagcc 
      aactcgagt aaatttccg tgcgaagtc attgaaaat 
      attcggaac tatatgttc aataaaggg tacctcaag 
      gatgatatt gagaagcaa gttgaggag aataaaatt 
      gatattgaa aaggctgaa cgagacgca atagggaaa 
      aacctgatc tacactcag accgaaatg gaaaagtgt 
      ttggaacgt tttaatatt aaagacgaa agagaagac 
      ccaactttc cagaaggaa tacgtgaaa gaaccattg 
      gtgattcca gaacacaat gttccatca ttcgggata 
      caacctgtt aataacgaa tgcccctac cctataaca 
      acaccgccg attccgagc cttgattgc aaaagtgaa 
      gcgcaacgg attgcatcc actgagtac gacacggag 
      gaggaaaaa atcaagaaa tattacgac aaagtattg 
      cgcgaacac aaacacatt tgtgagaat gctgaacca 
      caacagccg ccaccagaa gacgactgt ctggcaaaa 
      gaacgcaaa gggaaaagt gtaaaattt gatgataac 
      gtaaaatgt gaataa
    mRNA sequence
    • >TC014663 RA
      tgaaaatgg caggtttgc cgaaattgc cggggctca 
      ccttcactg accccactc tgaccagtt accacctat 
      cccagacct ttagcattt caaatgggt ataaaatcc 
      caaagaacg actacgtgg ggataggaa atcgggaac 
      tggactcaa attcggtca aattcatta gtaccaacg 
      acccggtcc gcttcgatc cctccctga cttatggac 
      gaaccaaag caattcctg tccaaatgt tcaaaccgc 
      attacgccc tttatgacc aaaagtgtc tcactttta 
      aggcttttt tcaagcagg gggttgcag agtcgcccg 
      atgagtact acagagtga ggcatgtga acataatct 
      actttctgg aagacgata cgatcacga ttatggaac 
      cccgaaccc caaatagtg ggattgatc aaggcaggc 
      ttttgaaac gacaccgaa ttcccaaaa gcgaagacg 
      gggaattct ggcattgga aggactttg gtgtgggca 
      gaaatattg ttttctacg gagttgtct atcatttgg 
      tggattgcg atactttta caagggaat atatggcca 
      gtcagggat tggtaatgg ctgaacctg agcaattac 
      cgcctgatc cctacaccc aaaatagat taatgaagt 
      cgcatcaga ataatttta ctaaaacgc cttcggcgg 
      atgataaat ttagacgct ttctggaat atgacggca 
      aaattctca aatttaaag cgatttggg acgacagag 
      actcggaat atggtgaaa taaggaaat acgaagttt 
      tatattttc tagcagatg acactgttg ccgtcaaag 
      aaattcaca ccaaaaatg atggccggg atccttact 
      cccaacttt tacgcaaaa ctaaattac caaagattt 
      ggaccgaca gaccggccc acttcccct caatctatc 
      tggagacaa ccgacgaag aggtcacag agttctacc 
      agcctaaag acttacgcg tcggagaaa caattttcg 
      ttctagggc ggaaaatgc tcctttacg actgtgatg 
      actttacgc gtaactact ttaagaaag cgctctgca 
      tggagcagg gccccccta tggacattt cagagaagc 
      ctaaaccac cccccgaga agaaaattc cccctcatg 
      acggcatcg gtagcttgg aggattccc tccaaaaca 
      ccctaactt ttatgccaa aaccgccgc gaaaagacg 
      tggttaagc aaattctga acgcaaata aatatctca 
      gatacgaga tgaagatgg atgatatac atccagaag 
      attcaattc gtaggtttt tgttgctgt attctttgg 
      gtgacaaca cttgtaaaa ttttcgagc ccccgattg 
      aaaattcgg ggattattg gggggaagt atttgagga 
      gtactttgt tgccaaaac ctaacacta atccacttg 
      atcccgaat attactcgc cgattgatt tttatattg 
      gggctacga taactgtct ttcagcaga ggtttataa 
      tcacggggg ctgacttgt atgtgtatc gctacatgc 
      aagccaact cgagtaaat ttccgtgcg aagtcattg 
      aaaatattc ggaactata tgttcaata aagggtacc 
      tcaaggatg atattgaga agcaagttg aggagaata 
      aaattgata ttgaaaagg ctgaacgag acgcaatag 
      ggaaaaacc tgatctaca ctcagaccg aaatggaaa 
      agtgtttgg aacgtttta atattaaag acgaaagag 
      aagacccaa ctttccaga aggaatacg tgaaagaac 
      cattggtga ttccagaac acaatgttc catcattcg 
      ggatacaac ctgttaata acgaatgcc cctacccta 
      taacaacac cgccgattc cgagccttg attgcaaaa 
      gtgaagcgc aacggattg catccactg agtacgaca 
      cggaggagg aaaaaatca agaaatatt acgacaaag 
      tattgcgcg aacacaaac acatttgtg agaatgctg 
      aaccacaac agccgccac cagaagacg actgtctgg 
      caaaagaac gcaaaggga aaagtgtaa aatttgatg 
      ataacgtaa aatgtgaat aataaattt ctgtaaata 
      tatttcgc