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Gene information
General information
TC014635 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 4756883..4757882
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014635
    iBeetle screen iB_02316
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014635 RA
      MGLLFSKLW SFFGNEEHK IVIVGLDNA 
      GKTTILYQF LMNEVVHTS PTIGSNVEE 
      VVWRNIHFI MWDLGGQQS LRAAWSTYY 
      TNTEFVILV IDSTDKERL SVIREELYK 
      MLANEELSK AGVLIYANK QDVKGSMSA 
      SEISKELDL TSIKQQQWH IQACCALTG 
      EGLYQGLEW IVSRLKKK
    CDS sequence
    • >TC014635 RA
      atgggtttg ttgttttcc aaactgtgg agctttttc 
      ggtaacgaa gaacataaa atagtaata gttggacta 
      gacaatgca ggtaaaacg actattcta tatcagttc 
      ttaatgaat gaggttgtt catactagc cccacaatt 
      ggttcaaat gttgaggaa gttgtttgg cgtaatatt 
      cattttata atgtgggat ttagggggt caacagtca 
      ttaagagca gcttggagt acttattat acaaacaca 
      gaatttgtg atactagta atagattca acggataag 
      gaacggttg agtgttatt agggaagaa ttgtacaaa 
      atgttggcg aatgaagaa ctgtcgaaa gcgggagtg 
      ctcatttat gcaaacaaa caggacgtt aaagggtca 
      atgagtgcg tctgaaata tccaaagaa ttagaccta 
      acttcaatt aaacaacaa caatggcac atacaagct 
      tgttgtgcg ttgacaggc gagggactt taccagggt 
      ttggaatgg attgtcagt cgcttgaaa aagaagtga
    mRNA sequence
    • >TC014635 RA
      tgcgtgaag agaaattgc ttgtcttct tttaattat 
      tcgttaaca tatgattgc aaacatcga acgtcgagt 
      ttcttccaa caattagaa ggaaattac ggtacctga 
      tttacttta gaacaatgg gtttgttgt tttccaaac 
      tgtggagct ttttcggta acgaagaac ataaaatag 
      taatagttg gactagaca atgcaggta aaacgacta 
      ttctatatc agttcttaa tgaatgagg ttgttcata 
      ctagcccca caattggtt caaatgttg aggaagttg 
      tttggcgta atattcatt ttataatgt gggatttag 
      ggggtcaac agtcattaa gagcagctt ggagtactt 
      attatacaa acacagaat ttgtgatac tagtaatag 
      attcaacgg ataaggaac ggttgagtg ttattaggg 
      aagaattgt acaaaatgt tggcgaatg aagaactgt 
      cgaaagcgg gagtgctca tttatgcaa acaaacagg 
      acgttaaag ggtcaatga gtgcgtctg aaatatcca 
      aagaattag acctaactt caattaaac aacaacaat 
      ggcacatac aagcttgtt gtgcgttga caggcgagg 
      gactttacc agggtttgg aatggattg tcagtcgct 
      tgaaaaaga agtgaccgg gttttccgc aaattactg 
      ttattttct catattgag aaatgcggt tgtgtaaca 
      tttctcata attcatgct tgtggtata attattcga 
      atttttatt aaggcatat ttacacaaa acttacgtg 
      ccacaagta aattcttgt taagccact aataataat 
      aaattgagt atttaagt