Status
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Gene information
General information
TC014633 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12644727..12650621
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014633
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02315

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014633 RA
      MAATWNNEY YIALEGRDL QANAMAVDN SGTYVLLAG 
      RRYIAIRNL DEDLEVVQK FPRQSKYDV GAAEWNPTN 
      HNRELCVIS SNQRLEVLT WRSQELIQT HSLRAHTRV 
      ITDLNWHRS DPSLLASCS VDTFIHIWD LRDARRPSL 
      SLSAIAEAS QVRWNKISS HLLATAHDG DIKIWDQRK 
      GTAPVQYVA AHLSKIHGL DWNPNVETQ LATSSQDNT 
      VKFFDMNNP RRAEYVLTT NAPVWRARF TPFGNGLVT 
      VVVPQLRRG ENSLLLWNT ANRSTPMHT FVGHRDVVL 
      EFEWRKQRA GSLDFQLIT WSKDQTLLV WKIEPFLQK 
      LCGHEPDET REEIAISNE SSEVVKIKK KTSPKIQPL 
      QQEFSLLNV HIPNLEVTA MDPVNRTCT VFANVYNFS 
      VYLQVSFPN AYPHGVPPT FQILHNSSV TEAAGAQLL 
      QTLNHLAQQ RVSKNRTCL ESCLRQMVT TLEQLSADS 
      ENDRIFDNS YTEPNVFGG YNDAYIPFP RTSGAKFCS 
      VGILVCFGR APHTRRFSG KVEATTPRA LSALETVFS 
      KRSSDHVTV SAYYYQKQR LRVKQHSRN ASKAIVHVY 
      DVSGLLLTN RQLAEEYIL DGDIATICK HNAASAAVV 
      GRWDLVQAW TLAELIACT RNKDEEWYN HPFGNQLSE 
      ALISHYAQH SDIQMAAML SCIFGKNEV NSSHQSKKS 
      LKYTNDTSP YHAIPPMDL PSDEWVFPT LKHMRSTSL 
      DNLRLNEIK NLLPKKNPS SSLHEYFKL SYAETLHRW 
      GFLYNRAEV LKYMCGPPD KHEGVEFFT HCKNCLKPD 
      LYGKHCTCS NCKKFSLNC IICHLSVRG SANCCIVCG 
      HGGHTKHLK QWFEKRDVC PSGCGCRCL TETATIFAT
    CDS sequence
    • >TC014633 RA
      atggctgcg acctggaac aatgagtat tacatcgct 
      ttggaagga agagatttg caagcaaat gctatggct 
      gttgataat tcaggaact tatgtttta ttagcaggg 
      cggaggtat attgcaata agaaattta gatgaagat 
      ctggaagtt gttcaaaaa tttccaaga cagagcaaa 
      tatgatgtt ggagccgct gaatggaac cctactaat 
      cacaaccgg gagctttgt gtgatatcg agcaatcag 
      cgcttggag gtcctgact tggcgttcg caagagcta 
      atccagacc cactccctc cgtgcccac actcgcgtc 
      ataaccgac ttaaactgg caccgcagc gaccccagc 
      cttctggcc tcctgttcc gtggacact ttcatccac 
      atctgggat ttgcgcgac gctcgccgt ccctcacta 
      tccctctct gcaatagca gaagcctct caagtgcga 
      tggaacaaa atctcatcg catctgctt gccactgca 
      cacgatggc gatatcaaa atttgggat caacgcaaa 
      ggaaccgct ccagtccag tacgtggcg gcgcattta 
      tcgaaaatc cacggcctg gactggaat cccaacgtc 
      gagacgcaa ttggcgacg tccagtcag gacaatact 
      gtgaaattt ttcgatatg aataatcca cgaagggct 
      gagtatgtt ctgacgacg aatgcgccc gtgtggagg 
      gcgaggttt acgccgttt gggaacggg ctggtgacg 
      gtggtggtg ccgcagctg cgaaggggt gaaaatagc 
      ttgttgctg tggaatacg gccaatagg tccactccc 
      atgcatacg tttgtgggg cacagggat gtggttttg 
      gagtttgag tggaggaag cagagggcg gggtcgttg 
      gattttcaa ttaattacg tggtcgaag gatcagacg 
      ttgttagtc tggaaaatc gagccgttc ctgcagaag 
      ttatgcggc cacgagccg gacgaaacc cgcgaagaa 
      atcgccatc agcaacgaa tcgtccgaa gtggtcaaa 
      atcaagaaa aaaacctca ccaaaaatc cagcccttg 
      cagcaggaa ttctccctt ctaaacgtc cacatcccc 
      aacctggag gtcacagcc atggacccc gtaaaccgc 
      acttgcacc gtcttcgcc aacgtctac aacttctcc 
      gtttacttg caagtctca ttccccaac gcttatccc 
      cacggcgtt cctccaacg ttccaaatc ctgcacaac 
      tcatcggtg accgaagcc gcaggcgcc caactcctc 
      caaaccctc aaccacttg gcccaacaa agagtatca 
      aaaaaccgc acttgtttg gagtcgtgc ctccgccag 
      atggtcaca accctcgaa cagctttcg gcggattcg 
      gaaaacgac cgaatcttt gataattcg tacacggaa 
      ccgaacgtt ttcggcggt tataacgac gcctacatt 
      ccgtttccg cgcacttcg ggggccaag ttttgttcg 
      gtggggatt ctggtgtgt ttcgggcgc gctccgcac 
      actaggagg ttctcaggg aaggtggag gcgacgact 
      ccgagggcc ttgtcggct ctggagacg gtgttttcc 
      aagaggtcg agtgatcat gtgaccgtg tctgcgtat 
      tactaccag aagcaaagg ttgagagtg aagcaacat 
      tcgaggaat gcctccaag gcgattgta catgtgtat 
      gatgtttcc gggctgtta ctgacgaat agacagttg 
      gcagaggag tatattctg gatggggat attgccacg 
      atttgcaaa cataatgcg gcaagtgct gcggttgtg 
      ggacgctgg gatttggtt caggcgtgg acgctggct 
      gaacttatt gcctgtaca aggaataag gacgaggag 
      tggtacaat catccgttt gggaatcag ctttcggaa 
      gccctaatt tcgcattat gcccaacac tctgatatc 
      cagatggca gccatgctg tcgtgcata tttgggaaa 
      aacgaagtc aactcttct caccaatca aaaaaatcg 
      ctcaaatat acgaacgac acatccccc tatcacgca 
      attcccccc atggaccta ccttccgac gaatgggta 
      tttcccaca ttgaagcac atgcgctcc acatcactg 
      gacaatctt cgtctaaac gaaatcaaa aacctttta 
      ccaaagaaa aatccgtct tcgtcgcta cacgaatat 
      ttcaagctc tcgtacgct gaaaccctt cacaggtgg 
      ggtttcttg tacaaccgt gccgaagtc ctgaagtac 
      atgtgcggg cctccagac aaacacgaa ggggttgaa 
      tttttcacc cattgtaag aattgtcta aagccggac 
      ttgtacggc aaacattgt acgtgtagt aattgtaaa 
      aaattttcg ctgaattgt attatatgc cacttgtct 
      gtccgagga tcggcgaat tgttgcatc gtttgtggg 
      catggggga cacactaaa catttgaaa cagtggttt 
      gagaaacgc gatgtttgc ccgtcgggc tgcgggtgc 
      aggtgcctt acagagaca gccacgatt tttgccact 
      taa
    mRNA sequence
    • >TC014633 RA
      tgcagagtg cctaagtcc atttctaat aacagagcg 
      tgtctttta gggtgtaaa ttagttttg aaataagca 
      tacaaattg agacacacc atggctgcg acctggaac 
      aatgagtat tacatcgct ttggaagga agagatttg 
      caagcaaat gctatggct gttgataat tcaggaact 
      tatgtttta ttagcaggg cggaggtat attgcaata 
      agaaattta gatgaagat ctggaagtt gttcaaaaa 
      tttccaaga cagagcaaa tatgatgtt ggagccgct 
      gaatggaac cctactaat cacaaccgg gagctttgt 
      gtgatatcg agcaatcag cgcttggag gtcctgact 
      tggcgttcg caagagcta atccagacc cactccctc 
      cgtgcccac actcgcgtc ataaccgac ttaaactgg 
      caccgcagc gaccccagc cttctggcc tcctgttcc 
      gtggacact ttcatccac atctgggat ttgcgcgac 
      gctcgccgt ccctcacta tccctctct gcaatagca 
      gaagcctct caagtgcga tggaacaaa atctcatcg 
      catctgctt gccactgca cacgatggc gatatcaaa 
      atttgggat caacgcaaa ggaaccgct ccagtccag 
      tacgtggcg gcgcattta tcgaaaatc cacggcctg 
      gactggaat cccaacgtc gagacgcaa ttggcgacg 
      tccagtcag gacaatact gtgaaattt ttcgatatg 
      aataatcca cgaagggct gagtatgtt ctgacgacg 
      aatgcgccc gtgtggagg gcgaggttt acgccgttt 
      gggaacggg ctggtgacg gtggtggtg ccgcagctg 
      cgaaggggt gaaaatagc ttgttgctg tggaatacg 
      gccaatagg tccactccc atgcatacg tttgtgggg 
      cacagggat gtggttttg gagtttgag tggaggaag 
      cagagggcg gggtcgttg gattttcaa ttaattacg 
      tggtcgaag gatcagacg ttgttagtc tggaaaatc 
      gagccgttc ctgcagaag ttatgcggc cacgagccg 
      gacgaaacc cgcgaagaa atcgccatc agcaacgaa 
      tcgtccgaa gtggtcaaa atcaagaaa aaaacctca 
      ccaaaaatc cagcccttg cagcaggaa ttctccctt 
      ctaaacgtc cacatcccc aacctggag gtcacagcc 
      atggacccc gtaaaccgc acttgcacc gtcttcgcc 
      aacgtctac aacttctcc gtttacttg caagtctca 
      ttccccaac gcttatccc cacggcgtt cctccaacg 
      ttccaaatc ctgcacaac tcatcggtg accgaagcc 
      gcaggcgcc caactcctc caaaccctc aaccacttg 
      gcccaacaa agagtatca aaaaaccgc acttgtttg 
      gagtcgtgc ctccgccag atggtcaca accctcgaa 
      cagctttcg gcggattcg gaaaacgac cgaatcttt 
      gataattcg tacacggaa ccgaacgtt ttcggcggt 
      tataacgac gcctacatt ccgtttccg cgcacttcg 
      ggggccaag ttttgttcg gtggggatt ctggtgtgt 
      ttcgggcgc gctccgcac actaggagg ttctcaggg 
      aaggtggag gcgacgact ccgagggcc ttgtcggct 
      ctggagacg gtgttttcc aagaggtcg agtgatcat 
      gtgaccgtg tctgcgtat tactaccag aagcaaagg 
      ttgagagtg aagcaacat tcgaggaat gcctccaag 
      gcgattgta catgtgtat gatgtttcc gggctgtta 
      ctgacgaat agacagttg gcagaggag tatattctg 
      gatggggat attgccacg atttgcaaa cataatgcg 
      gcaagtgct gcggttgtg ggacgctgg gatttggtt 
      caggcgtgg acgctggct gaacttatt gcctgtaca 
      aggaataag gacgaggag tggtacaat catccgttt 
      gggaatcag ctttcggaa gccctaatt tcgcattat 
      gcccaacac tctgatatc cagatggca gccatgctg 
      tcgtgcata tttgggaaa aacgaagtc aactcttct 
      caccaatca aaaaaatcg ctcaaatat acgaacgac 
      acatccccc tatcacgca attcccccc atggaccta 
      ccttccgac gaatgggta tttcccaca ttgaagcac 
      atgcgctcc acatcactg gacaatctt cgtctaaac 
      gaaatcaaa aacctttta ccaaagaaa aatccgtct 
      tcgtcgcta cacgaatat ttcaagctc tcgtacgct 
      gaaaccctt cacaggtgg ggtttcttg tacaaccgt 
      gccgaagtc ctgaagtac atgtgcggg cctccagac 
      aaacacgaa ggggttgaa tttttcacc cattgtaag 
      aattgtcta aagccggac ttgtacggc aaacattgt 
      acgtgtagt aattgtaaa aaattttcg ctgaattgt 
      attatatgc cacttgtct gtccgagga tcggcgaat 
      tgttgcatc gtttgtggg catggggga cacactaaa 
      catttgaaa cagtggttt gagaaacgc gatgtttgc 
      ccgtcgggc tgcgggtgc aggtgcctt acagagaca 
      gccacgatt tttgccact taaatattt attttactt 
      gtgaacgta aatagttca agtccatta actcaacaa 
      aaacataaa ggactcaat attcttgaa aaagccgct 
      tttaattcg aattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY