Status
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Gene information
General information
TC034705 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12526812..12532762
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034705
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02314

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034705 RA
      MSVGHQAKR QEDEEESDA PAPPDGGWG WMVVFGSFM 
IHIITDGVT YSFGVFYDE FLDYFQEGK AATAWVLSI 
LVGVTLCSG PISSSFVNR WGCRPVTIA GAIIGSICM 
IISVFAQNV TTLYFTIGI GTGFGFGLI YLPAIVSVT 
TYFEKKRSL ATGIAVCGS GFGTFIFAP IITRLLVEY 
GWRGAMLII AGIVLECIV FGALFRPLE TEEDKNLKP 
PPKITLKSI EAKVDLHVS EHNLQQIST MNGNGEIHR 
PHSLGHFSM PRGTKLLEP SKNISNGKT YEASRLALS 
QPMLALADR PFQHRYGSQ NLRKHGPLD KPDVFYQGS 
LMNIPSYRS RLDLKNREE MAFLGRRQS SISYRRRHV 
DTNKSQTTM CGCIPCSQE TKDTLKEML DFSLFHDWI 
FILFITSNF LTSIGFNIP YVYIVPKAK SMNLSAKEA 
SMLLSIIGF ANTIGRIVL GYFSDKAWV NRLMVYNWC 
LTLCGIATT FSAFCPTFY TLALFASVF GFTIGAYVG 
LTSVILVDL LGLERLTNA FGLLLLFQG IASLIGPPM 
AGALYDATL SYDPGFYLS GITISLSGV ILFFIPPIQ 
RCKDRKQER NPTDI
    CDS sequence
    • >TC034705 RA
      atgtcagtg ggtcatcaa gcgaaaagg caggaagat 
gaggaagaa agcgatgca ccggcaccc ccggacggg 
ggctggggc tggatggtg gtctttggc tcttttatg 
atacacatt atcactgat ggggtaaca tattccttt 
ggagttttc tacgacgaa ttcttagat tatttccaa 
gagggaaag gcagccaca gcgtgggtt ctttccatc 
ctagttggt gtaacactt tgttcaggt ccaatttca 
agttccttt gttaaccga tggggctgc cgtccagtg 
accatcgcc ggcgccata atcggcagc atttgcatg 
ataatatcc gtatttgcc caaaacgtc acaaccctc 
tacttcacc atcggcata ggaacaggc ttcggtttc 
ggcctcatc taccttcca gcaatagtc agcgtcacc 
acctatttc gaaaagaaa cgctctctt gccaccggc 
atagcagtc tgtggctca ggctttggc accttcatt 
ttcgctcct ataataacc cgactactt gtggagtac 
ggctggagg ggcgccatg ttaattata gccggcata 
gtcctggaa tgcattgtc ttcggcgcc ctgttccgg 
cctctggag accgaagaa gacaagaac ttgaaacca 
ccaccaaaa atcaccctc aaatcgatc gaagccaaa 
gtggacttg cacgtgtcc gaacacaac ttgcagcag 
atttcgaca atgaacgga aacggggaa atacacaga 
ccgcacagt ttgggccac ttttccatg ccgcgaggc 
accaaactc ctggagccc agcaagaac atatccaat 
gggaagacg tacgaggcg tccagactt gccctaagc 
cagccgatg ctggccctg gccgacagg cccttccag 
caccggtac gggagccag aatttgcgg aaacatggg 
cctttggac aagccggat gtgttctac cagggcagt 
ttaatgaac ataccgtcg tacaggtcg cggcttgat 
ttgaaaaat cgggaagaa atggctttt ctgggccga 
cgacagtcg tcaattagt tacaggagg cgacatgtg 
gacactaac aagtcgcaa acgacaatg tgtgggtgc 
attccctgc tcgcaagaa accaaagac acgctgaag 
gagatgcta gactttagc cttttccac gactggatt 
ttcattctc ttcatcacg agcaatttt ctgacgagc 
attggcttt aatataccg tacgtttac atcgtgccg 
aaggccaaa tcgatgaac ttgtcggcg aaagaagcg 
agcatgttg ctgtccatt atcgggttt gctaacacg 
attgggagg atcgttttg gggtacttt tccgataag 
gcttgggtt aatcgcctt atggtttac aactggtgc 
ctaacactt tgtggaata gccacgact tttagtgcc 
ttttgtccc acgttctac actttggca ctatttgcc 
agtgtgttt ggttttacg attggagcg tacgttgga 
ctcacgtct gttatcctg gtcgatttg ctaggactc 
gagcgactt acgaatgcc ttcgggctt ttgctttta 
tttcaaggg attgcttcg cttattgga ccccccatg 
gcaggtgcc ttgtatgat gcaacgctt tcgtacgat 
cccggattc tatctgtct ggaattacg atttctctc 
agtggggtc atcctgttc ttcattcct ccaatccag 
cggtgtaaa gacagaaag caggaacgg aatcccact 
gatatatag
    mRNA sequence
    • >TC034705 RA
      cccggattg tggaaaata agggattat ttattattg 
atgttttca aaatattgc atcaaggat aatcagata 
cggcatcag atggagggg cagtacttt attaccaca 
acgataaac atgacaaaa ttagtagcg gcgtcggca 
acgtcggta tcagaagta gcagagtcc caaacgcga 
cacttgtgt ccagatcca cgtgctgcg ggagtgttt 
ttattttag tcccgattc gaaaatgtc agtgggtca 
tcaagcgaa aaggcagga agatgagga agaaagcga 
tgcaccggc acccccgga cgggggctg gggctggat 
ggtggtctt tggctcttt tatgataca cattatcac 
tgatggggt aacatattc ctttggagt tttctacga 
cgaattctt agattattt ccaagaggg aaaggcagc 
cacagcgtg ggttctttc catcctagt tggtgtaac 
actttgttc aggtccaat ttcaagttc ctttgttaa 
ccgatgggg ctgccgtcc agtgaccat cgccggcgc 
cataatcgg cagcatttg catgataat atccgtatt 
tgcccaaaa cgtcacaac cctctactt caccatcgg 
cataggaac aggcttcgg tttcggcct catctacct 
tccagcaat agtcagcgt caccaccta tttcgaaaa 
gaaacgctc tcttgccac cggcatagc agtctgtgg 
ctcaggctt tggcacctt cattttcgc tcctataat 
aacccgact acttgtgga gtacggctg gaggggcgc 
catgttaat tatagccgg catagtcct ggaatgcat 
tgtcttcgg cgccctgtt ccggcctct ggagaccga 
agaagacaa gaacttgaa accaccacc aaaaatcac 
cctcaaatc gatcgaagc caaagtgga cttgcacgt 
gtccgaaca caacttgca gcagatttc gacaatgaa 
cggaaacgg ggaaataca cagaccgca cagtttggg 
ccacttttc catgccgcg aggcaccaa actcctgga 
gcccagcaa gaacatatc caatgggaa gacgtacga 
ggcgtccag acttgccct aagccagcc gatgctggc 
cctggccga caggccctt ccagcaccg gtacgggag 
ccagaattt gcggaaaca tgggccttt ggacaagcc 
ggatgtgtt ctaccaggg cagtttaat gaacatacc 
gtcgtacag gtcgcggct tgatttgaa aaatcggga 
agaaatggc ttttctggg ccgacgaca gtcgtcaat 
tagttacag gaggcgaca tgtggacac taacaagtc 
gcaaacgac aatgtgtgg gtgcattcc ctgctcgca 
agaaaccaa agacacgct gaaggagat gctagactt 
tagcctttt ccacgactg gattttcat tctcttcat 
cacgagcaa ttttctgac gagcattgg ctttaatat 
accgtacgt ttacatcgt gccgaaggc caaatcgat 
gaacttgtc ggcgaaaga agcgagcat gttgctgtc 
cattatcgg gtttgctaa cacgattgg gaggatcgt 
tttggggta cttttccga taaggcttg ggttaatcg 
ccttatggt ttacaactg gtgcctaac actttgtgg 
aatagccac gacttttag tgccttttg tcccacgtt 
ctacacttt ggcactatt tgccagtgt gtttggttt 
tacgattgg agcgtacgt tggactcac gtctgttat 
cctggtcga tttgctagg actcgagcg acttacgaa 
tgccttcgg gcttttgct tttatttca agggattgc 
ttcgcttat tggaccccc catggcagg tgccttgta 
tgatgcaac gctttcgta cgatcccgg attctatct 
gtctggaat tacgatttc tctcagtgg ggtcatcct 
gttcttcat tcctccaat ccagcggtg taaagacag 
aaagcagga acggaatcc cactgatat atagcaatt 
actgaggcc tcactttga ggaggattt tagatgtta 
taatactat ttaattttt tttattaca tatcagtca 
gtctagact ggatttgat gtgccataa ttgtctgca 
atgcagatc gatacagtg taagagttg attccggat 
aagtgttca gtgatttca ggaaaatag tgtgattga 
cattaatgt tatgtggat gcatgtggt gcgaacatt 
tttataatt taaaacttg ttatatcca tttacgcta 
gtgtaaata ttgtaaata attaaaaca tcggagacc 
aaatacatt ttaaactta agt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY