| General information | |||
| TC034705 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 12526812..12532762 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034705 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02314 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC034705 RA
MSVGHQAKR QEDEEESDA PAPPDGGWG WMVVFGSFM IHIITDGVT YSFGVFYDE FLDYFQEGK AATAWVLSI LVGVTLCSG PISSSFVNR WGCRPVTIA GAIIGSICM IISVFAQNV TTLYFTIGI GTGFGFGLI YLPAIVSVT TYFEKKRSL ATGIAVCGS GFGTFIFAP IITRLLVEY GWRGAMLII AGIVLECIV FGALFRPLE TEEDKNLKP PPKITLKSI EAKVDLHVS EHNLQQIST MNGNGEIHR PHSLGHFSM PRGTKLLEP SKNISNGKT YEASRLALS QPMLALADR PFQHRYGSQ NLRKHGPLD KPDVFYQGS LMNIPSYRS RLDLKNREE MAFLGRRQS SISYRRRHV DTNKSQTTM CGCIPCSQE TKDTLKEML DFSLFHDWI FILFITSNF LTSIGFNIP YVYIVPKAK SMNLSAKEA SMLLSIIGF ANTIGRIVL GYFSDKAWV NRLMVYNWC LTLCGIATT FSAFCPTFY TLALFASVF GFTIGAYVG LTSVILVDL LGLERLTNA FGLLLLFQG IASLIGPPM AGALYDATL SYDPGFYLS GITISLSGV ILFFIPPIQ RCKDRKQER NPTDI
- >TC034705 RA
atgtcagtg ggtcatcaa gcgaaaagg caggaagat gaggaagaa agcgatgca ccggcaccc ccggacggg ggctggggc tggatggtg gtctttggc tcttttatg atacacatt atcactgat ggggtaaca tattccttt ggagttttc tacgacgaa ttcttagat tatttccaa gagggaaag gcagccaca gcgtgggtt ctttccatc ctagttggt gtaacactt tgttcaggt ccaatttca agttccttt gttaaccga tggggctgc cgtccagtg accatcgcc ggcgccata atcggcagc atttgcatg ataatatcc gtatttgcc caaaacgtc acaaccctc tacttcacc atcggcata ggaacaggc ttcggtttc ggcctcatc taccttcca gcaatagtc agcgtcacc acctatttc gaaaagaaa cgctctctt gccaccggc atagcagtc tgtggctca ggctttggc accttcatt ttcgctcct ataataacc cgactactt gtggagtac ggctggagg ggcgccatg ttaattata gccggcata gtcctggaa tgcattgtc ttcggcgcc ctgttccgg cctctggag accgaagaa gacaagaac ttgaaacca ccaccaaaa atcaccctc aaatcgatc gaagccaaa gtggacttg cacgtgtcc gaacacaac ttgcagcag atttcgaca atgaacgga aacggggaa atacacaga ccgcacagt ttgggccac ttttccatg ccgcgaggc accaaactc ctggagccc agcaagaac atatccaat gggaagacg tacgaggcg tccagactt gccctaagc cagccgatg ctggccctg gccgacagg cccttccag caccggtac gggagccag aatttgcgg aaacatggg cctttggac aagccggat gtgttctac cagggcagt ttaatgaac ataccgtcg tacaggtcg cggcttgat ttgaaaaat cgggaagaa atggctttt ctgggccga cgacagtcg tcaattagt tacaggagg cgacatgtg gacactaac aagtcgcaa acgacaatg tgtgggtgc attccctgc tcgcaagaa accaaagac acgctgaag gagatgcta gactttagc cttttccac gactggatt ttcattctc ttcatcacg agcaatttt ctgacgagc attggcttt aatataccg tacgtttac atcgtgccg aaggccaaa tcgatgaac ttgtcggcg aaagaagcg agcatgttg ctgtccatt atcgggttt gctaacacg attgggagg atcgttttg gggtacttt tccgataag gcttgggtt aatcgcctt atggtttac aactggtgc ctaacactt tgtggaata gccacgact tttagtgcc ttttgtccc acgttctac actttggca ctatttgcc agtgtgttt ggttttacg attggagcg tacgttgga ctcacgtct gttatcctg gtcgatttg ctaggactc gagcgactt acgaatgcc ttcgggctt ttgctttta tttcaaggg attgcttcg cttattgga ccccccatg gcaggtgcc ttgtatgat gcaacgctt tcgtacgat cccggattc tatctgtct ggaattacg atttctctc agtggggtc atcctgttc ttcattcct ccaatccag cggtgtaaa gacagaaag caggaacgg aatcccact gatatatag
- >TC034705 RA
cccggattg tggaaaata agggattat ttattattg atgttttca aaatattgc atcaaggat aatcagata cggcatcag atggagggg cagtacttt attaccaca acgataaac atgacaaaa ttagtagcg gcgtcggca acgtcggta tcagaagta gcagagtcc caaacgcga cacttgtgt ccagatcca cgtgctgcg ggagtgttt ttattttag tcccgattc gaaaatgtc agtgggtca tcaagcgaa aaggcagga agatgagga agaaagcga tgcaccggc acccccgga cgggggctg gggctggat ggtggtctt tggctcttt tatgataca cattatcac tgatggggt aacatattc ctttggagt tttctacga cgaattctt agattattt ccaagaggg aaaggcagc cacagcgtg ggttctttc catcctagt tggtgtaac actttgttc aggtccaat ttcaagttc ctttgttaa ccgatgggg ctgccgtcc agtgaccat cgccggcgc cataatcgg cagcatttg catgataat atccgtatt tgcccaaaa cgtcacaac cctctactt caccatcgg cataggaac aggcttcgg tttcggcct catctacct tccagcaat agtcagcgt caccaccta tttcgaaaa gaaacgctc tcttgccac cggcatagc agtctgtgg ctcaggctt tggcacctt cattttcgc tcctataat aacccgact acttgtgga gtacggctg gaggggcgc catgttaat tatagccgg catagtcct ggaatgcat tgtcttcgg cgccctgtt ccggcctct ggagaccga agaagacaa gaacttgaa accaccacc aaaaatcac cctcaaatc gatcgaagc caaagtgga cttgcacgt gtccgaaca caacttgca gcagatttc gacaatgaa cggaaacgg ggaaataca cagaccgca cagtttggg ccacttttc catgccgcg aggcaccaa actcctgga gcccagcaa gaacatatc caatgggaa gacgtacga ggcgtccag acttgccct aagccagcc gatgctggc cctggccga caggccctt ccagcaccg gtacgggag ccagaattt gcggaaaca tgggccttt ggacaagcc ggatgtgtt ctaccaggg cagtttaat gaacatacc gtcgtacag gtcgcggct tgatttgaa aaatcggga agaaatggc ttttctggg ccgacgaca gtcgtcaat tagttacag gaggcgaca tgtggacac taacaagtc gcaaacgac aatgtgtgg gtgcattcc ctgctcgca agaaaccaa agacacgct gaaggagat gctagactt tagcctttt ccacgactg gattttcat tctcttcat cacgagcaa ttttctgac gagcattgg ctttaatat accgtacgt ttacatcgt gccgaaggc caaatcgat gaacttgtc ggcgaaaga agcgagcat gttgctgtc cattatcgg gtttgctaa cacgattgg gaggatcgt tttggggta cttttccga taaggcttg ggttaatcg ccttatggt ttacaactg gtgcctaac actttgtgg aatagccac gacttttag tgccttttg tcccacgtt ctacacttt ggcactatt tgccagtgt gtttggttt tacgattgg agcgtacgt tggactcac gtctgttat cctggtcga tttgctagg actcgagcg acttacgaa tgccttcgg gcttttgct tttatttca agggattgc ttcgcttat tggaccccc catggcagg tgccttgta tgatgcaac gctttcgta cgatcccgg attctatct gtctggaat tacgatttc tctcagtgg ggtcatcct gttcttcat tcctccaat ccagcggtg taaagacag aaagcagga acggaatcc cactgatat atagcaatt actgaggcc tcactttga ggaggattt tagatgtta taatactat ttaattttt tttattaca tatcagtca gtctagact ggatttgat gtgccataa ttgtctgca atgcagatc gatacagtg taagagttg attccggat aagtgttca gtgatttca ggaaaatag tgtgattga cattaatgt tatgtggat gcatgtggt gcgaacatt tttataatt taaaacttg ttatatcca tttacgcta gtgtaaata ttgtaaata attaaaaca tcggagacc aaatacatt ttaaactta agt
