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Gene information
General information
TC014607 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12335604..12336912
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014607
    iBeetle screen iB_02311

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014607 RA
      MEPDEKHKS QKPAPTDDP ECIYRIYNV LSYFIVLVV 
      IGIPVWWYT TRVYRASLP LDRMFDVTL ANKTDKQFG 
      IPLSLEYDV LITIVNPDP ENLHVELAG EDLDANLQP 
      FLSKISPIA DFIVKSQWL YLTELGAIP RKIIDKYVL 
      YEEQLPHVI TPLEKKLWS HLSPRPCLN LVLYVSHCN 
      VPLFIRNSR DEDTANAFF SPRWGGIYI INPDQNSCY 
      VKNFKPDVQ LITATFISQ LGKMLKMGG TTERDVQDL 
      MRRKAEDMV DSTRRTLKS LAQLLSEIN SIVISDDVA 
      LKIQIAVDN ADVAEDLLG KNDVENALN FAKVAFKNA 
      EAAFSDPSL LALLYFPDD QKYAVYIPL FLPVMIPVL 
      MSLITVKKW YKEKKLKTE
    CDS sequence
    • >TC014607 RA
      atggaaccg gacgaaaaa cacaagagc caaaagccg 
      gcccctaca gacgatccg gaatgcatt tatcgcatt 
      tacaacgtc ctctcttac ttcatcgtc ctcgtagtg 
      atcggaatc ccggtctgg tggtacacc acccgggtc 
      tacagggcg agtctgccc ctggaccgc atgttcgac 
      gtgaccctc gccaacaaa accgacaaa cagttcgga 
      atccccctt agcctcgaa tacgacgtc cttatcaca 
      attgtaaac ccggaccca gaaaaccta cacgtggag 
      ctcgccggt gaggacctt gacgcgaat ctccagccc 
      ttcctttcc aaaatatcc ccaatcgcg gatttcatc 
      gtcaagtcg cagtggctg tatttgacc gagctgggg 
      gccatcccg cgcaaaatc atcgacaag tacgtactg 
      tacgaggag caactcccg cacgttata acccccttg 
      gagaagaaa ctctggtcg catctatcg ccccgaccg 
      tgcttgaac ctcgtcttg tacgtctcg cactgcaat 
      gtgcccctg tttatacga aacagtcgg gatgaagac 
      accgctaac gcgtttttc agccccagg tggggcggc 
      atttacatc ataaacccg gatcagaat tcttgctat 
      gttaaaaac ttcaaaccc gatgtgcaa ctcatcacg 
      gcgacgttt atatcgcaa ttagggaag atgctgaaa 
      atggggggc accacagag agggatgtt caagatttg 
      atgagacgc aaagctgaa gatatggtc gactcgact 
      cgcaggacg ttaaaatca ctggcacag cttttatcg 
      gaaataaac agtattgtg ataagcgat gacgtggcg 
      ctgaaaata caaattgcg gtggataat gctgacgtt 
      gctgaagac ttgttgggg aaaaatgat gttgaaaat 
      gcgctaaat tttgccaaa gttgcgttt aagaatgcc 
      gaggccgcg ttcagtgac ccgtcttta ctggctttg 
      ctttacttc cctgacgat caaaagtat gccgtttac 
      atccctctg ttcttacct gtaatgatc ccagtgctg 
      atgtcttta ataactgtg aaaaagtgg tacaaagag 
      aagaagtta aagactgaa taa
    mRNA sequence
    • >TC014607 RA
      acacatttg acagatgtt tgagtgaat cttaacctt 
      aaaaatgga accggacga aaaacacaa gagccaaaa 
      gccggcccc tacagacga tccggaatg catttatcg 
      catttacaa cgtcctctc ttacttcat cgtcctcgt 
      agtgatcgg aatcccggt ctggtggta caccacccg 
      ggtctacag ggcgagtct gcccctgga ccgcatgtt 
      cgacgtgac cctcgccaa caaaaccga caaacagtt 
      cggaatccc ccttagcct cgaatacga cgtccttat 
      cacaattgt aaacccgga cccagaaaa cctacacgt 
      ggagctcgc cggtgagga ccttgacgc gaatctcca 
      gcccttcct ttccaaaat atccccaat cgcggattt 
      catcgtcaa gtcgcagtg gctgtattt gaccgagct 
      gggggccat cccgcgcaa aatcatcga caagtacgt 
      actgtacga ggagcaact cccgcacgt tataacccc 
      cttggagaa gaaactctg gtcgcatct atcgccccg 
      accgtgctt gaacctcgt cttgtacgt ctcgcactg 
      caatgtgcc cctgtttat acgaaacag tcgggatga 
      agacaccgc taacgcgtt tttcagccc caggtgggg 
      cggcattta catcataaa cccggatca gaattcttg 
      ctatgttaa aaacttcaa acccgatgt gcaactcat 
      cacggcgac gtttatatc gcaattagg gaagatgct 
      gaaaatggg gggcaccac agagaggga tgttcaaga 
      tttgatgag acgcaaagc tgaagatat ggtcgactc 
      gactcgcag gacgttaaa atcactggc acagctttt 
      atcggaaat aaacagtat tgtgataag cgatgacgt 
      ggcgctgaa aatacaaat tgcggtgga taatgctga 
      cgttgctga agacttgtt ggggaaaaa tgatgttga 
      aaatgcgct aaattttgc caaagttgc gtttaagaa 
      tgccgaggc cgcgttcag tgacccgtc tttactggc 
      tttgcttta cttccctga cgatcaaaa gtatgccgt 
      ttacatccc tctgttctt acctgtaat gatcccagt 
      gctgatgtc tttaataac tgtgaaaaa gtggtacaa 
      agagaagaa gttaaagac tgaataatt ttttatttt 
      aaataaaac aagctttac aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY