Status
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Gene information
General information
TC014602 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12276812..12279734
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014602
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02309

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014602 RA
      MADVSKAQK LASARKKFK ELKKEKKKN TAQKAPELV 
      ESPPTSREQ TPVEGLTNE PAQLPNYFS NVNFNNGYQ 
      QPTAFFDNF SQNNDPFAC ITPSNNLNG DQNHAFSVP 
      EDNKNDEIS NFQAFDSVK FDQKPSPRP QLSTVESLR 
      QLQDQMAEL ISPPENEPL GGESPLEIR NQELAELLN 
      QEKVKNEEL NASLREYQT KLSQLEAEL KDKQLNTDT 
      RVEHEVSTL REELQCHVQ TVGILVAEK AELVANLSQ 
      FQLAAKQKT AECEELQGR LKTSRSRVA DLEREVALL 
      KNEKSQYDN MGREQNSEL EKLKQSYQS VKDEKEELL 
      QDLLEAREK LKSSSESCV EFQQQIKEL SNQLSMANI 
      KIQQLTTGD QDSSEIEKL TQEKFALEK QVADLSQTV 
      KTLSKEREE SSLQYQQYA QQLNAQLSS LATRLETSQ 
      KENESLAIR EQDRIKHIG ELEKQLQNL QNDQVSFAV 
      QKPTTETKA ELEAALELV ERLKTENVE LQEENVKAA 
      NEKELLLKE LEAKGESIS ELERIVEEL RTNQPDSGK 
      LLAIMESDK VAAARAVSQ NTELKNQMD SMQEVFMKL 
      NNDKIELTE KLTIEQRNN KELFEKLQK TELHLQTLA 
      DAIEIKDKE LSHLRENSS ELDKQLLQS EQLNDRLRH 
      YEAQDHSSQ ALQNELFQA NQTIEKLNE EIKQLKVCS 
      SDVKELREN ETKDATDSN HNGIVLDKE AAMKHLEEK 
      FLKTMEDIA NLTEEKQRL EHLVLQLQG ETETIGEYV 
      ALYQQQRSL LKQKAIEKD QQLSQLAND RELMKAKLD 
      KLNELVKKL VLEKGAVPS ELLEHHHDN HLCEEHAKI 
      SNEINKIAQ NTFTVEPSD TQSTETAEE IIALLSEIK 
      TSNLVQPAE NIHHCPWCS GQLITV
    CDS sequence
    • >TC014602 RA
      atggcagat gtgtcaaaa gcccaaaaa ttggcatct 
      gctagaaaa aagttcaag gaattaaaa aaggaaaaa 
      aagaagaac actgcccag aaagcaccc gaacttgtc 
      gagtcacca cccacaagt cgcgagcag acacctgtg 
      gaaggtctc acaaatgaa ccagcgcaa cttccaaat 
      tacttcagt aatgtaaac ttcaacaac ggctatcag 
      cagccaacg gcatttttc gataatttt tcacaaaat 
      aacgaccct ttcgcgtgc atcacgccc tcaaataat 
      ctcaatggt gaccaaaac cacgcgttt tcagtacct 
      gaagacaat aaaaatgac gaaatttcc aacttccaa 
      gcctttgac agcgtcaaa tttgaccag aaaccttcc 
      ccacgcccc cagttgtcc acagttgag agtctgcgc 
      cagttgcag gaccaaatg gccgaatta ataagcccc 
      cctgaaaat gagccactg gggggcgag tcaccgcta 
      gaaattcgg aatcaagag ttggcggaa ctgctcaat 
      caggaaaag gtgaaaaat gaagaactg aacgctagt 
      ttgagagaa tatcaaacg aagctcagc caattagag 
      gccgagctt aaagacaaa caattgaac accgatact 
      agagtagag cacgaagtt agcactctt cgagaagag 
      ttacaatgt catgtccaa actgtggga attctcgtg 
      gcggaaaag gccgaatta gttgcaaat ctcagccaa 
      ttccagttg gcggcgaaa cagaaaaca gccgaatgc 
      gaggagtta caaggtcgc ttgaaaact tccagatca 
      cgtgtcgca gaccttgaa cgtgaagtt gcgctcctt 
      aagaacgaa aagtcgcaa tacgacaac atgggacgc 
      gaacaaaac tccgagttg gaaaagttg aaacagagc 
      taccagtct gtgaaagat gaaaaagag gaactgctc 
      caggatttg cttgaagct cgagaaaaa ctcaaaagt 
      agctctgaa agttgcgtc gagttccaa cagcagatc 
      aaggagttg tccaatcaa ctgtccatg gccaatatt 
      aaaatccag caactcaca actggggat caagacagc 
      agtgagatc gagaaactg actcaggag aagttcgct 
      cttgagaaa caagtcgcc gatttgagc caaacagtt 
      aaaacttta tcgaaagag agggaggag tctagtcta 
      cagtaccag caatatgcc caacagttg aacgcgcaa 
      ttgtcgagt ctggcgact cgattagag acgtctcag 
      aaggagaac gaatctctg gcgatacgc gaacaggac 
      aggattaaa cacatcggc gagttggag aaacaactg 
      caaaatttg cagaacgat caagtgtcc tttgccgtg 
      caaaaaccc accactgaa acaaaagcc gaactcgaa 
      gccgcccta gaattagtc gagagactc aaaaccgaa 
      aacgtggag ttgcaagag gagaatgtt aaagcagca 
      aacgaaaag gagttgctt ttgaaggag ttggaagcc 
      aaaggagag agtataagt gagctggag cggatcgta 
      gaggagttg aggaccaat cagcccgac agtggaaaa 
      ttattagca attatggaa agtgacaaa gtcgcagcg 
      gccagagcc gtgtctcaa aacaccgaa ttgaagaat 
      cagatggac agtatgcag gaagttttc atgaagttg 
      aataatgac aaaatcgaa ttgaccgaa aaactgaca 
      atcgagcag cgcaacaat aaggaattg ttcgaaaaa 
      ttgcaaaaa acagagctg catttgcaa actttggcc 
      gacgcgatt gaaatcaaa gataaggag ttgtcgcat 
      cttcgggag aactcgtcc gagctcgac aaacagttg 
      ctgcagagt gaacagttg aacgatagg ttgaggcat 
      tacgaggca caggatcat agttcacaa gcgttacaa 
      aacgagctg tttcaagcc aatcaaact atcgaaaaa 
      ctgaacgaa gagataaaa caattgaaa gtgtgtagt 
      agtgacgta aaagagtta cgagagaat gagacaaaa 
      gatgccact gatagcaac cacaatgga attgttttg 
      gacaaagag gcggccatg aaacatttg gaagagaaa 
      tttttgaag acgatggaa gacattgcc aatttaaca 
      gaggagaag caacgtctc gaacacctg gtgttgcaa 
      ttgcaaggt gagaccgag acgatagga gagtatgtg 
      gcgctctat caacaacag aggagtcta ttaaaacaa 
      aaagcgatt gaaaaagac cagcaattg tcacagtta 
      gccaatgac cgcgaattg atgaaagcc aagctagac 
      aaattgaac gaattggtg aaaaagctg gtccttgaa 
      aaaggagcc gttccgtcc gaattgctg gaacatcac 
      cacgacaat catctatgc gaagaacac gcgaaaatc 
      agtaacgaa atcaataaa atcgcgcag aatacgttc 
      acagtcgaa ccgagtgat actcaaagt accgaaaca 
      gcagaagaa ataatcgca ctcctgtcg gagattaaa 
      accagtaac ttggtacaa ccggctgag aatatacat 
      cattgtccg tggtgttcg ggccaactt attacggtg 
      taa
    mRNA sequence
    • >TC014602 RA
      tttgacaca tgtcaacaa aaaatatgg cagatgtgt 
      caaaagccc aaaaattgg catctgcta gaaaaaagt 
      tcaaggaat taaaaaagg aaaaaaaga agaacactg 
      cccagaaag cacccgaac ttgtcgagt caccaccca 
      caagtcgcg agcagacac ctgtggaag gtctcacaa 
      atgaaccag cgcaacttc caaattact tcagtaatg 
      taaacttca acaacggct atcagcagc caacggcat 
      ttttcgata atttttcac aaaataacg accctttcg 
      cgtgcatca cgccctcaa ataatctca atggtgacc 
      aaaaccacg cgttttcag tacctgaag acaataaaa 
      atgacgaaa tttccaact tccaagcct ttgacagcg 
      tcaaatttg accagaaac cttccccac gcccccagt 
      tgtccacag ttgagagtc tgcgccagt tgcaggacc 
      aaatggccg aattaataa gcccccctg aaaatgagc 
      cactggggg gcgagtcac cgctagaaa ttcggaatc 
      aagagttgg cggaactgc tcaatcagg aaaaggtga 
      aaaatgaag aactgaacg ctagtttga gagaatatc 
      aaacgaagc tcagccaat tagaggccg agcttaaag 
      acaaacaat tgaacaccg atactagag tagagcacg 
      aagttagca ctcttcgag aagagttac aatgtcatg 
      tccaaactg tgggaattc tcgtggcgg aaaaggccg 
      aattagttg caaatctca gccaattcc agttggcgg 
      cgaaacaga aaacagccg aatgcgagg agttacaag 
      gtcgcttga aaacttcca gatcacgtg tcgcagacc 
      ttgaacgtg aagttgcgc tccttaaga acgaaaagt 
      cgcaatacg acaacatgg gacgcgaac aaaactccg 
      agttggaaa agttgaaac agagctacc agtctgtga 
      aagatgaaa aagaggaac tgctccagg atttgcttg 
      aagctcgag aaaaactca aaagtagct ctgaaagtt 
      gcgtcgagt tccaacagc agatcaagg agttgtcca 
      atcaactgt ccatggcca atattaaaa tccagcaac 
      tcacaactg gggatcaag acagcagtg agatcgaga 
      aactgactc aggagaagt tcgctcttg agaaacaag 
      tcgccgatt tgagccaaa cagttaaaa ctttatcga 
      aagagaggg aggagtcta gtctacagt accagcaat 
      atgcccaac agttgaacg cgcaattgt cgagtctgg 
      cgactcgat tagagacgt ctcagaagg agaacgaat 
      ctctggcga tacgcgaac aggacagga ttaaacaca 
      tcggcgagt tggagaaac aactgcaaa atttgcaga 
      acgatcaag tgtcctttg ccgtgcaaa aacccacca 
      ctgaaacaa aagccgaac tcgaagccg ccctagaat 
      tagtcgaga gactcaaaa ccgaaaacg tggagttgc 
      aagaggaga atgttaaag cagcaaacg aaaaggagt 
      tgcttttga aggagttgg aagccaaag gagagagta 
      taagtgagc tggagcgga tcgtagagg agttgagga 
      ccaatcagc ccgacagtg gaaaattat tagcaatta 
      tggaaagtg acaaagtcg cagcggcca gagccgtgt 
      ctcaaaaca ccgaattga agaatcaga tggacagta 
      tgcaggaag ttttcatga agttgaata atgacaaaa 
      tcgaattga ccgaaaaac tgacaatcg agcagcgca 
      acaataagg aattgttcg aaaaattgc aaaaaacag 
      agctgcatt tgcaaactt tggccgacg cgattgaaa 
      tcaaagata aggagttgt cgcatcttc gggagaact 
      cgtccgagc tcgacaaac agttgctgc agagtgaac 
      agttgaacg ataggttga ggcattacg aggcacagg 
      atcatagtt cacaagcgt tacaaaacg agctgtttc 
      aagccaatc aaactatcg aaaaactga acgaagaga 
      taaaacaat tgaaagtgt gtagtagtg acgtaaaag 
      agttacgag agaatgaga caaaagatg ccactgata 
      gcaaccaca atggaattg ttttggaca aagaggcgg 
      ccatgaaac atttggaag agaaatttt tgaagacga 
      tggaagaca ttgccaatt taacagagg agaagcaac 
      gtctcgaac acctggtgt tgcaattgc aaggtgaga 
      ccgagacga taggagagt atgtggcgc tctatcaac 
      aacagagga gtctattaa aacaaaaag cgattgaaa 
      aagaccagc aattgtcac agttagcca atgaccgcg 
      aattgatga aagccaagc tagacaaat tgaacgaat 
      tggtgaaaa agctggtcc ttgaaaaag gagccgttc 
      cgtccgaat tgctggaac atcaccacg acaatcatc 
      tatgcgaag aacacgcga aaatcagta acgaaatca 
      ataaaatcg cgcagaata cgttcacag tcgaaccga 
      gtgatactc aaagtaccg aaacagcag aagaaataa 
      tcgcactcc tgtcggaga ttaaaacca gtaacttgg 
      tacaaccgg ctgagaata tacatcatt gtccgtggt 
      gttcgggcc aacttatta cggtgtaaa tgggatatt 
      gtgattata ttgttattt taaatagct tgattaaag 
      tttattaca taac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY