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Gene information
General information
TC014601 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12274142..12276043
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014601
    iBeetle screen iB_02308

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 66.67% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014601 RA
      MGYKICMVS DFFFPNMGG VEEHIYNLS QCLLLEGHK 
      VVVMTHSYG DCVGIRYKT NGLKVYYLP IRVFYNQSI 
      LPTMICNVP LIRNILIRE QIEIIHGHS AFSALAHEA 
      LVIGNLMGL RTVFTDHSL FGFADASAV ITNKLLQMS 
      LTDCNHWIC VSHTGKENT VLRAKVSSK RVSVIPNAV 
      DTCAFMPDP SQRSSEFIT IVVVSRLVY RKGVDLTAQ 
      IISEMCSKH DKINFLIAG DGPKRGLLE EIRERQGLH 
      DRVTLLGSL EHSLVRNVL NQGHIFLNT SLTEAYCMA 
      IVEAVSCGL QVVSTRVGG IPEVLPEDL IYLTEPNVP 
      SLIKTLETA IDDLKNNKV NCPYLCNLR IKNYYNWEN 
      VSKRTEIVY QKVMQERNK TLQEQLWSY KTSGVWPFL 
      LAVSLCYLM LQFLEYIYP REYIDIAKD YNYKKKSKE 
      KIY
    CDS sequence
    • >TC014601 RA
      atgggttac aaaatttgt atggtttct gattttttc 
      ttccccaac atgggcggg gtcgaggaa cacatttat 
      aacctctcc cagtgtttg ctccttgag gggcacaaa 
      gttgtggtt atgacacac agttacggc gattgtgtc 
      ggaattcgt tacaaaacc aacggtctg aaggtctat 
      taccttcca attagggtg ttttataac cagtcgatt 
      ctgccgaca atgatatgc aatgtgccc ctaattcgc 
      aacattttg ataagggaa caaattgag ataattcac 
      ggccattcg gcattttca gcccttgca cacgaggcg 
      ctggtaatc ggaaattta atgggatta aggactgtc 
      tttacggac cacagtttg tttggtttt gctgacgct 
      agtgcggtt ataacaaat aaattgtta caaatgtcg 
      ttaacagat tgcaatcac tggatttgt gtttcacac 
      actgggaaa gaaaatacg gtgctgcgg gcaaaagtc 
      agcagtaaa agggtttct gtgattcca aacgcagtt 
      gatacatgt gcatttatg ccggatccc agccaaaga 
      tcgagcgaa tttatcaca atagtcgtg gtttcaaga 
      ctagtttac cgcaaaggc gtggatttg acggcccaa 
      attatttca gaaatgtgc agtaaacac gataaaatc 
      aacttttta atcgcaggc gacggaccc aagcgcgga 
      ttgcttgag gaaatacgg gaaaggcaa gggttgcac 
      gatagggtc acccttttg ggtagcctg gagcattcc 
      cttgttcgc aatgtacta aaccaaggg catattttc 
      ctcaacact tccctgact gaagcgtat tgtatggct 
      atagtagaa gcggtttca tgcggatta caagtcgtt 
      tcgacccga gttggggga atcccagaa gtgttgcct 
      gaagatttg atttacttg actgaacca aacgtccct 
      tctcttatt aaaaccctc gaaaccgca attgatgat 
      ttgaaaaat aacaaagtt aattgtccg tacttgtgt 
      aatttgagg ataaaaaat tattacaac tgggagaac 
      gtttcaaaa cggacggaa atcgtgtat cagaaagtt 
      atgcaagaa aggaataaa acattacag gagcaatta 
      tggagttac aaaacaagt ggtgtttgg ccattttta 
      ctggcggtc tctttgtgt tatctaatg ttgcagttt 
      ttggagtac atataccca agggaatac atcgatatc 
      gccaaggat tataattat aagaaaaaa agtaaagag 
      aagatttat tag
    mRNA sequence
    • >TC014601 RA
      ctaaaaatg tcaagataa cctcaaata tgggttaca 
      aaatttgta tggtttctg attttttct tccccaaca 
      tgggcgggg tcgaggaac acatttata acctctccc 
      agtgtttgc tccttgagg ggcacaaag ttgtggtta 
      tgacacaca gttacggcg attgtgtcg gaattcgtt 
      acaaaacca acggtctga aggtctatt accttccaa 
      ttagggtgt tttataacc agtcgattc tgccgacaa 
      tgatatgca atgtgcccc taattcgca acattttga 
      taagggaac aaattgaga taattcacg gccattcgg 
      cattttcag cccttgcac acgaggcgc tggtaatcg 
      gaaatttaa tgggattaa ggactgtct ttacggacc 
      acagtttgt ttggttttg ctgacgcta gtgcggtta 
      taacaaata aattgttac aaatgtcgt taacagatt 
      gcaatcact ggatttgtg tttcacaca ctgggaaag 
      aaaatacgg tgctgcggg caaaagtca gcagtaaaa 
      gggtttctg tgattccaa acgcagttg atacatgtg 
      catttatgc cggatccca gccaaagat cgagcgaat 
      ttatcacaa tagtcgtgg tttcaagac tagtttacc 
      gcaaaggcg tggatttga cggcccaaa ttatttcag 
      aaatgtgca gtaaacacg ataaaatca actttttaa 
      tcgcaggcg acggaccca agcgcggat tgcttgagg 
      aaatacggg aaaggcaag ggttgcacg atagggtca 
      cccttttgg gtagcctgg agcattccc ttgttcgca 
      atgtactaa accaagggc atattttcc tcaacactt 
      ccctgactg aagcgtatt gtatggcta tagtagaag 
      cggtttcat gcggattac aagtcgttt cgacccgag 
      ttgggggaa tcccagaag tgttgcctg aagatttga 
      tttacttga ctgaaccaa acgtccctt ctcttatta 
      aaaccctcg aaaccgcaa ttgatgatt tgaaaaata 
      acaaagtta attgtccgt acttgtgta atttgagga 
      taaaaaatt attacaact gggagaacg tttcaaaac 
      ggacggaaa tcgtgtatc agaaagtta tgcaagaaa 
      ggaataaaa cattacagg agcaattat ggagttaca 
      aaacaagtg gtgtttggc catttttac tggcggtct 
      ctttgtgtt atctaatgt tgcagtttt tggagtaca 
      tatacccaa gggaataca tcgatatcg ccaaggatt 
      ataattata agaaaaaaa gtaaagaga agatttatt 
      agttgtgtc ctgtaaata aaatttttt attaccaaa 
      ctgtctttc tctcatact tataattaa aacataagt 
      tcaagtctc agagtcaac tgtcttcag ttagttgat 
      gaaactatc aggtgaaga aattcttaa catcattgt 
      agataaata gaaaaaaca aaaatatta aacatgcac 
      acttacctc agagttttg tagtacagt ttgattttt 
      cctcatcgt caatgaact gcaaaaaaa ttaaacaca 
      ttatatcaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY