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Gene information
General information
TC014596 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12206435..12208151
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014596
    iBeetle screen iB_02307

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014596 RA
      MSRMWEFQK FKGLRFTDT FLQLFSEKN SDGTKKLRF 
      KANELEQRF QEQLLPADM VYVSTFLNA HPEVVEVDL 
      SYNGIEDEG LETLCNITL SHQNNLQHL NLSHCDITP 
      AGIKVLYFI AEKYGFVKL KTLRLTGNK LGPTGGHLT 
      ALFAPFCPT LEHLDVGDT DQTADSVDC LFLQLYRSQ 
      IKILDVSRV IPCNNMTQV NAVFLSESI SELIRLNDT 
      IVEIHAQKC SFDAHDVES LILGLKLNT SLKFLDIGY 
      NNICCVGVE FLSEWLKTR PQLLGLCIA GNNIKNHGG 
      VALSFGMPF SKLRYLDIS YNHIGNEGI IAILNSIKK 
      PYMLRYFLL WGNSFSHPA NKIIQRMML STVFEQRGI 
      DVKIYEVDG VLYAARYPV DKYKHRYYG VMDHGCAVG 
      LKIKRNRVE TPDDKPRAL VNFAHIDRY PEIEKKSKQ 
      GQATDEKEK KIAKCEDVV KNEEKSGGV DESN
    CDS sequence
    • >TC014596 RA
      atgtcgcga atgtgggaa ttccaaaaa ttcaaagga 
      ctgcgtttc accgacacg ttcctccaa ctgttctcg 
      gagaaaaac tccgacggg acgaaaaaa ctccgcttc 
      aaagccaac gaattagag caaagattc caagagcag 
      ttacttcca gctgacatg gtgtacgtc tccaccttt 
      ctaaacgct catcctgag gtggttgaa gttgatctg 
      agctacaac gggatcgag gatgagggt ttagagacc 
      ttgtgcaac atcaccctc tctcatcag aataacttg 
      caacacctg aacttatcc cactgcgac attacacca 
      gcggggatc aaagtcttg tatttcatt gcagagaaa 
      tacggtttc gtcaagttg aaaacattg agactcact 
      ggaaataaa ttgggaccc actggggga cacttaact 
      gcccttttc gcccctttt tgccccact ttggagcac 
      ctggatgtc ggagacacc gaccagact gcggacagt 
      gtcgactgt cttttcctc caactgtac cgcagccaa 
      atcaaaatc ctggacgta tctcgcgtg atcccttgc 
      aataacatg actcaagtc aatgctgtt tttttgagc 
      gaaagcatc tccgaactg atccgactg aatgacacc 
      attgttgaa atacatgcc caaaaatgc agttttgat 
      gctcatgac gttgaaagt ttgattctg ggattaaag 
      cttaacact tcgttgaaa ttcctcgat atcgggtat 
      aataatatt tgttgtgtc ggggttgag tttttatca 
      gagtggctt aaaacccgc ccccagctc ttggggttg 
      tgtattgcg ggaaataat atcaaaaat cacggtggt 
      gttgcgttg agttttgga atgccgttt agcaaattg 
      cgatatttg gatatcagt tataaccac atcggtaat 
      gaaggaatc attgctatt ttgaacagt attaagaag 
      ccctacatg ctaaggtat ttcctcctg tgggggaat 
      tccttcagt caccctgct aataaaatc atacagagg 
      atgatgtta tccacagtt ttcgaacaa aggggcata 
      gatgtgaag atttatgaa gttgatggg gtgttatat 
      gccgcacga taccctgtt gataaatat aaacatcgg 
      tattatggg gttatggat catgggtgt gccgtggga 
      ctgaagatt aaaagaaat agggtcgaa acaccggac 
      gataaaccg agagcgttg gtcaatttt gcgcacatt 
      gataggtat ccggaaatt gaaaaaaag tcaaaacaa 
      gggcaagca actgatgag aaagagaaa aaaattgca 
      aaatgcgaa gacgtggtg aaaaatgag gaaaagagt 
      ggaggcgtt gacgaatca aattaa
    mRNA sequence
    • >TC014596 RA
      gtgacgttg aaaacccaa atgccaaaa actgatttt 
      ttttctaaa cctccaaaa tgtcgcgaa tgtgggaat 
      tccaaaaat tcaaaggac tgcgtttca ccgacacgt 
      tcctccaac tgttctcgg agaaaaact ccgacggga 
      cgaaaaaac tccgcttca aagccaacg aattagagc 
      aaagattcc aagagcagt tacttccag ctgacatgg 
      tgtacgtct ccacctttc taaacgctc atcctgagg 
      tggttgaag ttgatctga gctacaacg ggatcgagg 
      atgagggtt tagagacct tgtgcaaca tcaccctct 
      ctcatcaga ataacttgc aacacctga acttatccc 
      actgcgaca ttacaccag cggggatca aagtcttgt 
      atttcattg cagagaaat acggtttcg tcaagttga 
      aaacattga gactcactg gaaataaat tgggaccca 
      ctgggggac acttaactg cccttttcg ccccttttt 
      gccccactt tggagcacc tggatgtcg gagacaccg 
      accagactg cggacagtg tcgactgtc ttttcctcc 
      aactgtacc gcagccaaa tcaaaatcc tggacgtat 
      ctcgcgtga tcccttgca ataacatga ctcaagtca 
      atgctgttt ttttgagcg aaagcatct ccgaactga 
      tccgactga atgacacca ttgttgaaa tacatgccc 
      aaaaatgca gttttgatg ctcatgacg ttgaaagtt 
      tgattctgg gattaaagc ttaacactt cgttgaaat 
      tcctcgata tcgggtata ataatattt gttgtgtcg 
      gggttgagt ttttatcag agtggctta aaacccgcc 
      cccagctct tggggttgt gtattgcgg gaaataata 
      tcaaaaatc acggtggtg ttgcgttga gttttggaa 
      tgccgttta gcaaattgc gatatttgg atatcagtt 
      ataaccaca tcggtaatg aaggaatca ttgctattt 
      tgaacagta ttaagaagc cctacatgc taaggtatt 
      tcctcctgt gggggaatt ccttcagtc accctgcta 
      ataaaatca tacagagga tgatgttat ccacagttt 
      tcgaacaaa ggggcatag atgtgaaga tttatgaag 
      ttgatgggg tgttatatg ccgcacgat accctgttg 
      ataaatata aacatcggt attatgggg ttatggatc 
      atgggtgtg ccgtgggac tgaagatta aaagaaata 
      gggtcgaaa caccggacg ataaaccga gagcgttgg 
      tcaattttg cgcacattg ataggtatc cggaaattg 
      aaaaaaagt caaaacaag ggcaagcaa ctgatgaga 
      aagagaaaa aaattgcaa aatgcgaag acgtggtga 
      aaaatgagg aaaagagtg gaggcgttg acgaatcaa 
      attaaagat tgtgcacaa aattgaata tttttttgc 
      tttcgtaac gcttttgta acgcaaact cgtgaaata 
      aataaatga catccttga at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY