Status
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Gene information
General information
TC033195
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014587,TC014588
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12140218..12143962
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033195
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02305

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033195 RA
      MRRPQSCDT FVVLPPLTQ HGVVFGKNS DRPQGEVQE 
      VVYVPATQS SEPVKCTYI EVESAGATK AVILSKPGW 
      MWGAEMGAN QCGVVIGNE AVWTGDNEG DHDPTVKRL 
      LGMDLVRLG LERGATAGE ALDVITQLL EKYGQGGPC 
      SHNDPNFTY HNSFLIADP KTAWVLETS GKHWAAVEV 
      TSGYRNISN VLTITTKID KKSEGLEEY ARSKGLWNG 
      EGEFNFCEA FSGEKKPGD ARYLAGEKL LAQHTSSNN 
      FKETDMFAI LRDKNSEIC RRCDAPFPT QGSQVSVLS 
      SSRPSVHWF TATPDPSVS VYKPFIFSP NAVISNHTK 
      CPESDKTAP HTLYSLHSQ AVKRGSDVQ TLLRNMEAD 
      CVKELEAVL ENVGDDLSE FDELLKDCV ETEWPLLNS 
      NVKMLRIKP LQVISKRFA CELKSILAA KIPKEQERI 
      KAFRKAHGK TKIGEVTVN MAYGGMRGI KGLICETSV 
      LDPHEGIRF RGLSIPECQ EKLPKAECG EQPLPEGLF 
      WLLLTGEVP TSEQVKSLS EEWASRSEL PAHVCKFLK 
      QVPKEVHPM AQLSAACSI CNTESIFKK SYASVPKGK 
      YWESIYEDC MNLIAKLTP IAALIYKHT FKGTDEIGT 
      IDSDKDWSL NFCRMLGFD NEEFVELMR LYLTIHSDH 
      EGGNVSAHT VHLVGSALS DPYLSYAAG MNGLAGPLH 
      GLANQEVLQ WLRNLQKEV GKDPTHDKI KEFIWKTLK 
      SGRVVPGYG HAVLRITDP RFTVQKQFA EKYLPDDPL 
      FKIVSLVFE VVPPILKEL GKVQNPWPN VDAHSGVLL 
      QYYGMTEMT YYTVLFAVS RALGVLACT VWDRALGLP 
      IERPKSIST ERLIKEVTG GDDKKGKKG KKCD
    CDS sequence
    • >TC033195 RA
      atgcgccgc ccccagtcg tgtgacacc ttcgtggtg 
      ttgccgccc ctaacacag cacggggtc gtcttcggc 
      aaaaactca gaccggcct caaggagag gtccaggaa 
      gtggtttac gtgcccgcg acccaatcc tcggaacct 
      gtcaaatgc acttacatc gaagtcgag tcggctggg 
      gccaccaaa gcggtcatc cttagcaag ccggggtgg 
      atgtggggc gccgaaatg ggggcaaac cagtgtggg 
      gtggtgatc gggaacgag gcggtctgg acgggcgac 
      aatgagggg gaccacgac cccacagtc aaaagactg 
      ctaggcatg gatttggtt cgactaggg ctggagaga 
      ggggccact gcgggcgaa gccctggac gttataaca 
      caattatta gaaaaatac gggcaaggg gggccttgc 
      tctcataac gaccctaat tttacctat cataattcg 
      tttctaata gctgatcct aagacggcc tgggtgctc 
      gaaacttcg gggaagcac tgggccgct gttgaagtc 
      acaagtggg tatagaaat atatcaaac gtgttgacc 
      attaccaca aaaatagat aaaaagtct gaagggcta 
      gaagagtac gctagatcc aaaggttta tggaatgga 
      gagggcgaa tttaatttt tgtgaggcc ttcagtggc 
      gaaaaaaag cccggcgat gcccgatat ttagccggc 
      gagaaacta ctagcgcag cacacaagt tcgaataat 
      ttcaaagag accgatatg tttgcaatt ctaagagac 
      aaaaattcg gagatttgc agacgctgt gacgcccct 
      ttcccgacg caaggcagc caggtctca gttttgagt 
      tcctcgcgt cctagcgtg cactggttc accgccacc 
      cccgatcct tccgtttcc gtttacaaa ccgtttatt 
      ttttcccca aatgcggtc atttccaac catacgaaa 
      tgtccagaa agcgacaaa acagcccca cataccctc 
      tactcgctc cattcccag gcggtgaaa aggggcagc 
      gacgttcag actttgctg aggaacatg gaggctgac 
      tgtgtgaag gagttggag gcggttttg gagaatgtc 
      ggtgatgat ctctccgaa ttcgacgaa ttgctcaag 
      gattgcgtc gagaccgaa tggcccctg ctcaattca 
      aacgtcaag atgcttcgc attaaaccg ctccaagta 
      atttccaag cgtttcgct tgcgaatta aagtcaatc 
      cttgccgcc aagatcccc aaagagcag gagcgcatc 
      aaagccttc cgtaaggcc catggcaag accaaaatc 
      ggcgaagtt accgtcaac atggcatac ggcggcatg 
      cggggcatc aagggcctc atctgcgag acctcggtc 
      ctggacccc catgaaggc atccgtttc cggggcctc 
      tccatcccc gagtgccag gagaagctc ccgaaggcc 
      gagtgtggc gaacagccg ttgcctgaa ggcttgttc 
      tggttgctc ctcaccggg gaagtcccc acaagtgag 
      caagtcaag tccctgtcg gaggagtgg gccagtcgc 
      tccgaattg ccggcacac gtgtgcaaa ttcctgaaa 
      caggtcccc aaggaagtg caccctatg gcgcagctc 
      tcggccgct tgctcgatt tgcaacact gagagtatt 
      ttcaaaaag tcgtacgcc tcggttccc aaggggaag 
      tactgggag agcatctac gaggattgc atgaatttg 
      attgcgaag cttacgccc atcgccgcg ctgatttac 
      aagcacacg ttcaaaggc acggacgag atagggacc 
      attgattcg gacaaggac tggagtctg aatttttgc 
      agaatgctg gggtttgac aacgaggag tttgtcgag 
      ttgatgcgc ttgtatttg actatacac agcgaccat 
      gaagggggc aatgtctcg gctcacacc gtgcatttg 
      gtggggtcc gcccttagc gacccctac ttgtcttat 
      gccgccggt atgaatggt ttggctgga ccattgcac 
      ggtctggca aaccaagaa gtactgcag tggctacgc 
      aatttgcaa aaagaagta gggaaagac cccactcat 
      gacaaaatc aaagaattc atttggaaa actctgaaa 
      tcgggtcgg gtggttccc ggctatggc cacgccgtg 
      ctgcgcatc accgatccc aggttcacc gttcagaag 
      caattcgct gagaagtat ctccccgat gacccgttg 
      ttcaaaatt gtgtcgttg gtgttcgag gtggttccc 
      ccgattttg aaggagttg gggaaagta cagaacccg 
      tggcccaat gttgatgcc cactccggt gtcctcttg 
      caatattac ggcatgact gaaatgacc tattacacc 
      gtgttgttt gcggtgtct cgtgccctg ggtgtgttg 
      gcttgcacg gtgtgggac cgcgctctc ggcctgccc 
      attgaacgg cccaagtcc atctccacc gaacgtctc 
      attaaagaa gtcacgggt ggcgacgac aaaaaaggg 
      aagaaaggg aaaaaatgt gactaa
    mRNA sequence
    • >TC033195 RA
      ttcaatttc aaccagctg gtggtgagt cagtcgctc 
      gccatttaa cccggatca gtgaccttt tctgtcaaa 
      atgcgccgc ccccagtcg tgtgacacc ttcgtggtg 
      ttgccgccc ctaacacag cacggggtc gtcttcggc 
      aaaaactca gaccggcct caaggagag gtccaggaa 
      gtggtttac gtgcccgcg acccaatcc tcggaacct 
      gtcaaatgc acttacatc gaagtcgag tcggctggg 
      gccaccaaa gcggtcatc cttagcaag ccggggtgg 
      atgtggggc gccgaaatg ggggcaaac cagtgtggg 
      gtggtgatc gggaacgag gcggtctgg acgggcgac 
      aatgagggg gaccacgac cccacagtc aaaagactg 
      ctaggcatg gatttggtt cgactaggg ctggagaga 
      ggggccact gcgggcgaa gccctggac gttataaca 
      caattatta gaaaaatac gggcaaggg gggccttgc 
      tctcataac gaccctaat tttacctat cataattcg 
      tttctaata gctgatcct aagacggcc tgggtgctc 
      gaaacttcg gggaagcac tgggccgct gttgaagtc 
      acaagtggg tatagaaat atatcaaac gtgttgacc 
      attaccaca aaaatagat aaaaagtct gaagggcta 
      gaagagtac gctagatcc aaaggttta tggaatgga 
      gagggcgaa tttaatttt tgtgaggcc ttcagtggc 
      gaaaaaaag cccggcgat gcccgatat ttagccggc 
      gagaaacta ctagcgcag cacacaagt tcgaataat 
      ttcaaagag accgatatg tttgcaatt ctaagagac 
      aaaaattcg gagatttgc agacgctgt gacgcccct 
      ttcccgacg caaggcagc caggtctca gttttgagt 
      tcctcgcgt cctagcgtg cactggttc accgccacc 
      cccgatcct tccgtttcc gtttacaaa ccgtttatt 
      ttttcccca aatgcggtc atttccaac catacgaaa 
      tgtccagaa agcgacaaa acagcccca cataccctc 
      tactcgctc cattcccag gcggtgaaa aggggcagc 
      gacgttcag actttgctg aggaacatg gaggctgac 
      tgtgtgaag gagttggag gcggttttg gagaatgtc 
      ggtgatgat ctctccgaa ttcgacgaa ttgctcaag 
      gattgcgtc gagaccgaa tggcccctg ctcaattca 
      aacgtcaag atgcttcgc attaaaccg ctccaagta 
      atttccaag cgtttcgct tgcgaatta aagtcaatc 
      cttgccgcc aagatcccc aaagagcag gagcgcatc 
      aaagccttc cgtaaggcc catggcaag accaaaatc 
      ggcgaagtt accgtcaac atggcatac ggcggcatg 
      cggggcatc aagggcctc atctgcgag acctcggtc 
      ctggacccc catgaaggc atccgtttc cggggcctc 
      tccatcccc gagtgccag gagaagctc ccgaaggcc 
      gagtgtggc gaacagccg ttgcctgaa ggcttgttc 
      tggttgctc ctcaccggg gaagtcccc acaagtgag 
      caagtcaag tccctgtcg gaggagtgg gccagtcgc 
      tccgaattg ccggcacac gtgtgcaaa ttcctgaaa 
      caggtcccc aaggaagtg caccctatg gcgcagctc 
      tcggccgct tgctcgatt tgcaacact gagagtatt 
      ttcaaaaag tcgtacgcc tcggttccc aaggggaag 
      tactgggag agcatctac gaggattgc atgaatttg 
      attgcgaag cttacgccc atcgccgcg ctgatttac 
      aagcacacg ttcaaaggc acggacgag atagggacc 
      attgattcg gacaaggac tggagtctg aatttttgc 
      agaatgctg gggtttgac aacgaggag tttgtcgag 
      ttgatgcgc ttgtatttg actatacac agcgaccat 
      gaagggggc aatgtctcg gctcacacc gtgcatttg 
      gtggggtcc gcccttagc gacccctac ttgtcttat 
      gccgccggt atgaatggt ttggctgga ccattgcac 
      ggtctggca aaccaagaa gtactgcag tggctacgc 
      aatttgcaa aaagaagta gggaaagac cccactcat 
      gacaaaatc aaagaattc atttggaaa actctgaaa 
      tcgggtcgg gtggttccc ggctatggc cacgccgtg 
      ctgcgcatc accgatccc aggttcacc gttcagaag 
      caattcgct gagaagtat ctccccgat gacccgttg 
      ttcaaaatt gtgtcgttg gtgttcgag gtggttccc 
      ccgattttg aaggagttg gggaaagta cagaacccg 
      tggcccaat gttgatgcc cactccggt gtcctcttg 
      caatattac ggcatgact gaaatgacc tattacacc 
      gtgttgttt gcggtgtct cgtgccctg ggtgtgttg 
      gcttgcacg gtgtgggac cgcgctctc ggcctgccc 
      attgaacgg cccaagtcc atctccacc gaacgtctc 
      attaaagaa gtcacgggt ggcgacgac aaaaaaggg 
      aagaaaggg aaaaaatgt gactaaatc ttttgtttc 
      aaataaaac ttcaaaacc gtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY