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Gene information
General information
TC014580 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12089661..12091621
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014580
    iBeetle screen iB_02304

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014580 RA
      MVESPPPEF IKTIEWDMM DKTKFFPLS MLSSFTVRC 
ALYPLTLIK TRLQVQKHN DIYTGMFDA YGKIYRYEG 
FSGLYRGFW VSSVQIISG VFYISVYEG VRHLLAQKN 
IDSRVRALI AGGSASIVG QTIIVPFDV LSQHLMMMG 
VQNGDKLAF NTLGIAIKP GTSKLALTL EIAKEIFRR 
DGIGGFYRG YMASLAAYV PNSALWWGF YHFYQDELH 
AVMPSWVSH LFIQTVAGT LGGFTTTII TNPLDVIRA 
RLQVQRIES MKCAFRDLW VEEGVRMFS KGLSARLIQ 
SATFSFSII LGYETIKRV SVNDEYKEY VRW
    • >TC014580 RB
      MFFRNFITG WYSEDMSTM VESPPPEFI KTIEWDMMD 
KTKFFPLSM LSSFTVRCA LYPLTLIKT RLQVQKHND 
IYTGMFDAY GKIYRYEGF SGLYRGFWV SSVQIISGV 
FYISVYEGV RHLLAQKNI DSRVRALIA GGSASIVGQ 
TIIVPFDVL SQHLMMMGV QNGDKLAFN TLGIAIKPG 
TSKLALTLE IAKEIFRRD GIGGFYRGY MASLAAYVP 
NSALWWGFY HFYQDELHA VMPSWVSHL FIQTVAGTL 
GGFTTTIIT NPLDVIRAR LQVQRIESM KCAFRDLWV 
EEGVRMFSK GLSARLIQS ATFSFSIIL GYETIKRVS 
VNDEYKEYV RW
    CDS sequence
    • >TC014580 RA
      atggtggaa tcaccaccc cccgagttc atcaaaacg 
atcgaatgg gacatgatg gacaagacg aaattcttc 
cccttatca atgcttagt tcgttcaca gtcaggtgt 
gccctttac cccctaacc ttgataaaa acgaggctg 
caagtccaa aaacataac gacatttac actggcatg 
tttgacgct tatgggaaa atttacaga tatgaaggg 
ttctccggg ctgtaccgc ggattttgg gtgtcctcg 
gtccaaata atcagcggc gttttctac atttcggtg 
tacgagggg gtccggcat ttactcgca caaaagaat 
atcgactcg agggtcaga gcgctgatc gccgggggc 
agtgcctca attgtgggc caaacgatc attgttcct 
ttcgacgtc ctcagccag catctgatg atgatgggg 
gtgcagaac ggcgacaag ttagctttc aatacctta 
ggaattgcc atcaagcca gggacgagc aaactggcc 
ctaacgctt gaaatagcc aaagagatt ttccgcagg 
gacggaatt ggggggttt tacaggggg tatatggcc 
agtttggcg gcctacgtc ccgaattcg gctctgtgg 
tgggggttt tatcacttc taccaagat gagttgcac 
gcagtgatg ccttcgtgg gtctcgcat ttgttcatc 
cagacagtg gcggggacg ttagggggc ttcaccacc 
acaattatc accaacccc ttggatgtg atcagggct 
agactgcaa gtgcagagg atagaatct atgaagtgc 
gctttcagg gacttgtgg gttgaggaa ggtgtcagg 
atgttctcc aaggggtta agtgcgaga ctcatccaa 
tctgccacg tttagtttt agcataatt ctcggttat 
gagactata aagcgcgtt tctgttaac gatgaatat 
aaagaatat gtccgttgg tga
    • >TC014580 RB
      atgtttttc cgcaacttt ataactggt tggtacagt 
gaagacatg tcgacgatg gtggaatca ccacccccc 
gagttcatc aaaacgatc gaatgggac atgatggac 
aagacgaaa ttcttcccc ttatcaatg cttagttcg 
ttcacagtc aggtgtgcc ctttacccc ctaaccttg 
ataaaaacg aggctgcaa gtccaaaaa cataacgac 
atttacact ggcatgttt gacgcttat gggaaaatt 
tacagatat gaagggttc tccgggctg taccgcgga 
ttttgggtg tcctcggtc caaataatc agcggcgtt 
ttctacatt tcggtgtac gagggggtc cggcattta 
ctcgcacaa aagaatatc gactcgagg gtcagagcg 
ctgatcgcc gggggcagt gcctcaatt gtgggccaa 
acgatcatt gttcctttc gacgtcctc agccagcat 
ctgatgatg atgggggtg cagaacggc gacaagtta 
gctttcaat accttagga attgccatc aagccaggg 
acgagcaaa ctggcccta acgcttgaa atagccaaa 
gagattttc cgcagggac ggaattggg gggttttac 
agggggtat atggccagt ttggcggcc tacgtcccg 
aattcggct ctgtggtgg gggttttat cacttctac 
caagatgag ttgcacgca gtgatgcct tcgtgggtc 
tcgcatttg ttcatccag acagtggcg gggacgtta 
gggggcttc accaccaca attatcacc aaccccttg 
gatgtgatc agggctaga ctgcaagtg cagaggata 
gaatctatg aagtgcgct ttcagggac ttgtgggtt 
gaggaaggt gtcaggatg ttctccaag gggttaagt 
gcgagactc atccaatct gccacgttt agttttagc 
ataattctc ggttatgag actataaag cgcgtttct 
gttaacgat gaatataaa gaatatgtc cgttggtga
    mRNA sequence
    • >TC014580 RA
      gtgtcaaag tacacaatg tactttata aataaaagt 
cgaaaagtc gtgtttggt tcactttag cgcaattaa 
aaaatgttt ttccgcaac tttataacg taacgacgc 
atttccaat aatgtcaac gaattttag cattaattt 
cccttgata gtggttggt acagtgaag acatgtcga 
cgatggtgg aatcaccac cccccgagt tcatcaaaa 
cgatcgaat gggacatga tggacaaga cgaaattct 
tccccttat caatgctta gttcgttca cagtcaggt 
gtgcccttt accccctaa ccttgataa aaacgaggc 
tgcaagtcc aaaaacata acgacattt acactggca 
tgtttgacg cttatggga aaatttaca gatatgaag 
ggttctccg ggctgtacc gcggatttt gggtgtcct 
cggtccaaa taatcagcg gcgttttct acatttcgg 
tgtacgagg gggtccggc atttactcg cacaaaaga 
atatcgact cgagggtca gagcgctga tcgccgggg 
gcagtgcct caattgtgg gccaaacga tcattgttc 
ctttcgacg tcctcagcc agcatctga tgatgatgg 
gggtgcaga acggcgaca agttagctt tcaatacct 
taggaattg ccatcaagc cagggacga gcaaactgg 
ccctaacgc ttgaaatag ccaaagaga ttttccgca 
gggacggaa ttggggggt tttacaggg ggtatatgg 
ccagtttgg cggcctacg tcccgaatt cggctctgt 
ggtgggggt tttatcact tctaccaag atgagttgc 
acgcagtga tgccttcgt gggtctcgc atttgttca 
tccagacag tggcgggga cgttagggg gcttcacca 
ccacaatta tcaccaacc ccttggatg tgatcaggg 
ctagactgc aagtgcaga ggatagaat ctatgaagt 
gcgctttca gggacttgt gggttgagg aaggtgtca 
ggatgttct ccaaggggt taagtgcga gactcatcc 
aatctgcca cgtttagtt ttagcataa ttctcggtt 
atgagacta taaagcgcg tttctgtta acgatgaat 
ataaagaat atgtccgtt ggtgatact gttgtagtt 
ttatattgt ataaaatga gattttatc gatgttgat 
ttttttgga gattgtttt agttgacct ccacttatt 
tatttctgt tgattttag cgcaagttc aaggctcct 
atacagtcg agataaaat gaccagtga gcgagtgta 
taagagccg tgcaagtca ggtgcaaat aaaactcaa 
aacagcata taattacgt tttatttaa aagaaacca 
taactatac taaaaacat cagcaagtc ctaacaata 
aaaaaggga taaaatgca acagttaca agaataact 
cctagaagc tcgtgctgt tggtaagag tggtgcagc 
aaaggtgtg gaattattt ttgtaaaaa cagcactta 
tcgagccaa aatttcaac tactaggcc atatgaagg 
tgtttttat cacaacaac agctagagg cacgaaata 
tgggtctgt gtgaacact aatcaacac taaatgggt 
gactttaac tacaccaca tggatttgc atggcacaa 
ggtttaaag tctttcaga gaatttgtt cgtctatat 
ttcttaaaa tgcaggtgt tccgactga accccacat 
tagaagtaa tgggacttc taataatga ttgttttta 
tca
    • >TC014580 RB
      gtgtcaaag tacacaatg tactttata aataaaagt 
cgaaaagtc gtgtttggt tcactttag cgcaattaa 
aaaatgttt ttccgcaac tttataact ggttggtac 
agtgaagac atgtcgacg atggtggaa tcaccaccc 
cccgagttc atcaaaacg atcgaatgg gacatgatg 
gacaagacg aaattcttc cccttatca atgcttagt 
tcgttcaca gtcaggtgt gccctttac cccctaacc 
ttgataaaa acgaggctg caagtccaa aaacataac 
gacatttac actggcatg tttgacgct tatgggaaa 
atttacaga tatgaaggg ttctccggg ctgtaccgc 
ggattttgg gtgtcctcg gtccaaata atcagcggc 
gttttctac atttcggtg tacgagggg gtccggcat 
ttactcgca caaaagaat atcgactcg agggtcaga 
gcgctgatc gccgggggc agtgcctca attgtgggc 
caaacgatc attgttcct ttcgacgtc ctcagccag 
catctgatg atgatgggg gtgcagaac ggcgacaag 
ttagctttc aatacctta ggaattgcc atcaagcca 
gggacgagc aaactggcc ctaacgctt gaaatagcc 
aaagagatt ttccgcagg gacggaatt ggggggttt 
tacaggggg tatatggcc agtttggcg gcctacgtc 
ccgaattcg gctctgtgg tgggggttt tatcacttc 
taccaagat gagttgcac gcagtgatg ccttcgtgg 
gtctcgcat ttgttcatc cagacagtg gcggggacg 
ttagggggc ttcaccacc acaattatc accaacccc 
ttggatgtg atcagggct agactgcaa gtgcagagg 
atagaatct atgaagtgc gctttcagg gacttgtgg 
gttgaggaa ggtgtcagg atgttctcc aaggggtta 
agtgcgaga ctcatccaa tctgccacg tttagtttt 
agcataatt ctcggttat gagactata aagcgcgtt 
tctgttaac gatgaatat aaagaatat gtccgttgg 
tgatactgt tgtagtttt atattgtat aaaatgaga 
ttttatcga tgttgattt ttttggaga ttgttttag 
ttgacctcc acttattta tttctgttg attttagcg 
caagttcaa ggctcctat acagtcgag ataaaatga 
ccagtgagc gagtgtata agagccgtg caagtcagg 
tgcaaataa aactcaaaa cagcatata attacgttt 
tatttaaaa gaaaccata actatacta aaaacatca 
gcaagtcct aacaataaa aaagggata aaatgcaac 
agttacaag aataactcc tagaagctc gtgctgttg 
gtaagagtg gtgcagcaa aggtgtgga attattttt 
gtaaaaaca gcacttatc gagccaaaa tttcaacta 
ctaggccat atgaaggtg tttttatca caacaacag 
ctagaggca cgaaatatg ggtctgtgt gaacactaa 
tcaacacta aatgggtga ctttaacta caccacatg 
gatttgcat ggcacaagg tttaaagtc tttcagaga 
atttgttcg tctatattt cttaaaatg caggtgttc 
cgactgaac cccacatta gaagtaatg ggacttcta 
ataatgatt gtttttatc a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY