| General information | |||
| TC014580 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 12089661..12091621 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014580 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02304 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC014580 RA
MVESPPPEF IKTIEWDMM DKTKFFPLS MLSSFTVRC ALYPLTLIK TRLQVQKHN DIYTGMFDA YGKIYRYEG FSGLYRGFW VSSVQIISG VFYISVYEG VRHLLAQKN IDSRVRALI AGGSASIVG QTIIVPFDV LSQHLMMMG VQNGDKLAF NTLGIAIKP GTSKLALTL EIAKEIFRR DGIGGFYRG YMASLAAYV PNSALWWGF YHFYQDELH AVMPSWVSH LFIQTVAGT LGGFTTTII TNPLDVIRA RLQVQRIES MKCAFRDLW VEEGVRMFS KGLSARLIQ SATFSFSII LGYETIKRV SVNDEYKEY VRW
- >TC014580 RB
MFFRNFITG WYSEDMSTM VESPPPEFI KTIEWDMMD KTKFFPLSM LSSFTVRCA LYPLTLIKT RLQVQKHND IYTGMFDAY GKIYRYEGF SGLYRGFWV SSVQIISGV FYISVYEGV RHLLAQKNI DSRVRALIA GGSASIVGQ TIIVPFDVL SQHLMMMGV QNGDKLAFN TLGIAIKPG TSKLALTLE IAKEIFRRD GIGGFYRGY MASLAAYVP NSALWWGFY HFYQDELHA VMPSWVSHL FIQTVAGTL GGFTTTIIT NPLDVIRAR LQVQRIESM KCAFRDLWV EEGVRMFSK GLSARLIQS ATFSFSIIL GYETIKRVS VNDEYKEYV RW
- >TC014580 RA
atggtggaa tcaccaccc cccgagttc atcaaaacg atcgaatgg gacatgatg gacaagacg aaattcttc cccttatca atgcttagt tcgttcaca gtcaggtgt gccctttac cccctaacc ttgataaaa acgaggctg caagtccaa aaacataac gacatttac actggcatg tttgacgct tatgggaaa atttacaga tatgaaggg ttctccggg ctgtaccgc ggattttgg gtgtcctcg gtccaaata atcagcggc gttttctac atttcggtg tacgagggg gtccggcat ttactcgca caaaagaat atcgactcg agggtcaga gcgctgatc gccgggggc agtgcctca attgtgggc caaacgatc attgttcct ttcgacgtc ctcagccag catctgatg atgatgggg gtgcagaac ggcgacaag ttagctttc aatacctta ggaattgcc atcaagcca gggacgagc aaactggcc ctaacgctt gaaatagcc aaagagatt ttccgcagg gacggaatt ggggggttt tacaggggg tatatggcc agtttggcg gcctacgtc ccgaattcg gctctgtgg tgggggttt tatcacttc taccaagat gagttgcac gcagtgatg ccttcgtgg gtctcgcat ttgttcatc cagacagtg gcggggacg ttagggggc ttcaccacc acaattatc accaacccc ttggatgtg atcagggct agactgcaa gtgcagagg atagaatct atgaagtgc gctttcagg gacttgtgg gttgaggaa ggtgtcagg atgttctcc aaggggtta agtgcgaga ctcatccaa tctgccacg tttagtttt agcataatt ctcggttat gagactata aagcgcgtt tctgttaac gatgaatat aaagaatat gtccgttgg tga
- >TC014580 RB
atgtttttc cgcaacttt ataactggt tggtacagt gaagacatg tcgacgatg gtggaatca ccacccccc gagttcatc aaaacgatc gaatgggac atgatggac aagacgaaa ttcttcccc ttatcaatg cttagttcg ttcacagtc aggtgtgcc ctttacccc ctaaccttg ataaaaacg aggctgcaa gtccaaaaa cataacgac atttacact ggcatgttt gacgcttat gggaaaatt tacagatat gaagggttc tccgggctg taccgcgga ttttgggtg tcctcggtc caaataatc agcggcgtt ttctacatt tcggtgtac gagggggtc cggcattta ctcgcacaa aagaatatc gactcgagg gtcagagcg ctgatcgcc gggggcagt gcctcaatt gtgggccaa acgatcatt gttcctttc gacgtcctc agccagcat ctgatgatg atgggggtg cagaacggc gacaagtta gctttcaat accttagga attgccatc aagccaggg acgagcaaa ctggcccta acgcttgaa atagccaaa gagattttc cgcagggac ggaattggg gggttttac agggggtat atggccagt ttggcggcc tacgtcccg aattcggct ctgtggtgg gggttttat cacttctac caagatgag ttgcacgca gtgatgcct tcgtgggtc tcgcatttg ttcatccag acagtggcg gggacgtta gggggcttc accaccaca attatcacc aaccccttg gatgtgatc agggctaga ctgcaagtg cagaggata gaatctatg aagtgcgct ttcagggac ttgtgggtt gaggaaggt gtcaggatg ttctccaag gggttaagt gcgagactc atccaatct gccacgttt agttttagc ataattctc ggttatgag actataaag cgcgtttct gttaacgat gaatataaa gaatatgtc cgttggtga
- >TC014580 RA
gtgtcaaag tacacaatg tactttata aataaaagt cgaaaagtc gtgtttggt tcactttag cgcaattaa aaaatgttt ttccgcaac tttataacg taacgacgc atttccaat aatgtcaac gaattttag cattaattt cccttgata gtggttggt acagtgaag acatgtcga cgatggtgg aatcaccac cccccgagt tcatcaaaa cgatcgaat gggacatga tggacaaga cgaaattct tccccttat caatgctta gttcgttca cagtcaggt gtgcccttt accccctaa ccttgataa aaacgaggc tgcaagtcc aaaaacata acgacattt acactggca tgtttgacg cttatggga aaatttaca gatatgaag ggttctccg ggctgtacc gcggatttt gggtgtcct cggtccaaa taatcagcg gcgttttct acatttcgg tgtacgagg gggtccggc atttactcg cacaaaaga atatcgact cgagggtca gagcgctga tcgccgggg gcagtgcct caattgtgg gccaaacga tcattgttc ctttcgacg tcctcagcc agcatctga tgatgatgg gggtgcaga acggcgaca agttagctt tcaatacct taggaattg ccatcaagc cagggacga gcaaactgg ccctaacgc ttgaaatag ccaaagaga ttttccgca gggacggaa ttggggggt tttacaggg ggtatatgg ccagtttgg cggcctacg tcccgaatt cggctctgt ggtgggggt tttatcact tctaccaag atgagttgc acgcagtga tgccttcgt gggtctcgc atttgttca tccagacag tggcgggga cgttagggg gcttcacca ccacaatta tcaccaacc ccttggatg tgatcaggg ctagactgc aagtgcaga ggatagaat ctatgaagt gcgctttca gggacttgt gggttgagg aaggtgtca ggatgttct ccaaggggt taagtgcga gactcatcc aatctgcca cgtttagtt ttagcataa ttctcggtt atgagacta taaagcgcg tttctgtta acgatgaat ataaagaat atgtccgtt ggtgatact gttgtagtt ttatattgt ataaaatga gattttatc gatgttgat ttttttgga gattgtttt agttgacct ccacttatt tatttctgt tgattttag cgcaagttc aaggctcct atacagtcg agataaaat gaccagtga gcgagtgta taagagccg tgcaagtca ggtgcaaat aaaactcaa aacagcata taattacgt tttatttaa aagaaacca taactatac taaaaacat cagcaagtc ctaacaata aaaaaggga taaaatgca acagttaca agaataact cctagaagc tcgtgctgt tggtaagag tggtgcagc aaaggtgtg gaattattt ttgtaaaaa cagcactta tcgagccaa aatttcaac tactaggcc atatgaagg tgtttttat cacaacaac agctagagg cacgaaata tgggtctgt gtgaacact aatcaacac taaatgggt gactttaac tacaccaca tggatttgc atggcacaa ggtttaaag tctttcaga gaatttgtt cgtctatat ttcttaaaa tgcaggtgt tccgactga accccacat tagaagtaa tgggacttc taataatga ttgttttta tca
- >TC014580 RB
gtgtcaaag tacacaatg tactttata aataaaagt cgaaaagtc gtgtttggt tcactttag cgcaattaa aaaatgttt ttccgcaac tttataact ggttggtac agtgaagac atgtcgacg atggtggaa tcaccaccc cccgagttc atcaaaacg atcgaatgg gacatgatg gacaagacg aaattcttc cccttatca atgcttagt tcgttcaca gtcaggtgt gccctttac cccctaacc ttgataaaa acgaggctg caagtccaa aaacataac gacatttac actggcatg tttgacgct tatgggaaa atttacaga tatgaaggg ttctccggg ctgtaccgc ggattttgg gtgtcctcg gtccaaata atcagcggc gttttctac atttcggtg tacgagggg gtccggcat ttactcgca caaaagaat atcgactcg agggtcaga gcgctgatc gccgggggc agtgcctca attgtgggc caaacgatc attgttcct ttcgacgtc ctcagccag catctgatg atgatgggg gtgcagaac ggcgacaag ttagctttc aatacctta ggaattgcc atcaagcca gggacgagc aaactggcc ctaacgctt gaaatagcc aaagagatt ttccgcagg gacggaatt ggggggttt tacaggggg tatatggcc agtttggcg gcctacgtc ccgaattcg gctctgtgg tgggggttt tatcacttc taccaagat gagttgcac gcagtgatg ccttcgtgg gtctcgcat ttgttcatc cagacagtg gcggggacg ttagggggc ttcaccacc acaattatc accaacccc ttggatgtg atcagggct agactgcaa gtgcagagg atagaatct atgaagtgc gctttcagg gacttgtgg gttgaggaa ggtgtcagg atgttctcc aaggggtta agtgcgaga ctcatccaa tctgccacg tttagtttt agcataatt ctcggttat gagactata aagcgcgtt tctgttaac gatgaatat aaagaatat gtccgttgg tgatactgt tgtagtttt atattgtat aaaatgaga ttttatcga tgttgattt ttttggaga ttgttttag ttgacctcc acttattta tttctgttg attttagcg caagttcaa ggctcctat acagtcgag ataaaatga ccagtgagc gagtgtata agagccgtg caagtcagg tgcaaataa aactcaaaa cagcatata attacgttt tatttaaaa gaaaccata actatacta aaaacatca gcaagtcct aacaataaa aaagggata aaatgcaac agttacaag aataactcc tagaagctc gtgctgttg gtaagagtg gtgcagcaa aggtgtgga attattttt gtaaaaaca gcacttatc gagccaaaa tttcaacta ctaggccat atgaaggtg tttttatca caacaacag ctagaggca cgaaatatg ggtctgtgt gaacactaa tcaacacta aatgggtga ctttaacta caccacatg gatttgcat ggcacaagg tttaaagtc tttcagaga atttgttcg tctatattt cttaaaatg caggtgttc cgactgaac cccacatta gaagtaatg ggacttcta ataatgatt gtttttatc a
