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Gene information
General information
TC014543 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11407841..11436294
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014543
    iBeetle screen iB_02300

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014543 RA
      MGCTVSAEE RAAIARTKQ IDQTLKEES QRAKRDVKL 
      LLLGAGESG KSTIVKQMK IIHESGFTS EDFKQYRPV 
      VYSNTIQSL VAILRAMPN LGISYGNNE REIDGKMVF 
      DVIQRMEDT EPFSEELLA AMKRLWADS GVQECFGRS 
      NEYQLNDSA KYFLDDLDR LGAKDYQPT EQDILRTRV 
      KTTGIVEVH FSFKNLNFK LFDVGGQRS ERKKWIHCF 
      EDVTAIIFC VAMSEYDQV LHEDETTNR MQESLKLFD 
      SICNNKWFT DTSIILFLN KKDLFEEKI RKSPLTICF 
      PEYAGAQEY GEAAAYIQA QFEAKNKST TKEIYCHMT 
      CATDTHNIQ FVFDAVTDV IIANNLRNC GLY
    CDS sequence
    • >TC014543 RA
      atggggtgc acggtttcg gcggaggag cgggcggcc 
      atcgcccga acgaagcag attgaccag acgctcaaa 
      gaggagagt cagagggcg aaaagagat gttaaactg 
      ttgctgtta ggcgccgga gaatcaggg aagagcacg 
      atagtcaaa caaatgaaa attatccac gaaagtggg 
      tttacaagc gaagacttc aaacaatac cgacctgtg 
      gtgtatagc aacactatt caatccctt gtggccatc 
      ctgcgggcc atgccaaac ctcggaatc agctacggc 
      aacaacgag cgagagatc gatggtaaa atggttttc 
      gacgttatc caaaggatg gaagacacg gaacctttt 
      tccgaagaa ctcctcgcc gccatgaag cggttatgg 
      gctgattcg ggggttcaa gaatgtttc ggtcgttcc 
      aacgaatac caactgaac gattccgct aaatatttc 
      ctggacgat ctggaccga ttaggtgcc aaagactat 
      caacctacg gaacaggac atcctccgc acaagagtc 
      aaaacgacg gggatcgtc gaagtgcat ttctcgttt 
      aaaaacctc aatttcaaa ttattcgat gtgggtggt 
      cagaggtca gaaagaaag aagtggatt cactgtttc 
      gaagacgtg acggcaatt attttctgc gtagcgatg 
      agcgaatat gatcaagtc ctgcacgag gatgaaacg 
      acaaaccga atgcaggag agcttgaag cttttcgat 
      tcgatctgt aacaacaag tggttcacg gacacgtct 
      ataatcctc ttccttaat aagaaagac ttgttcgag 
      gaaaaaatt aggaagtcg cctttaaca atatgtttc 
      cccgaatac gcaggtgcg caagagtat ggcgaagca 
      gcggcgtac atccaagca caattcgaa gcgaagaac 
      aaatcgaca acaaaagaa atctactgc catatgacc 
      tgtgccaca gacacgcat aatatccag ttcgtattc 
      gacgcagtc actgatgtg atcatagct aataatctg 
      cgcaactgc ggcctctac tga
    mRNA sequence
    • >TC014543 RA
      cgatgaaaa tgaaccatc tgccgatgg ccacttcga 
      tctgtcgct tcaatcttt ctcgaatca gttagcgtt 
      gccaaagcc ggagcaagt gcgcttcct ctccggaaa 
      cccgaatag aaaaatagc gcgtttccg ggccccgga 
      ctattccaa atcacagtg gattaataa gtgcttcga 
      aaccatagt gcagtgatg ttgtagcgg tctgttttg 
      ggtggtcgg tcgtttttt ctcgttgtc ggagtgaaa 
      taaagccgc caagacgtc gtgcatcga ggactgcgc 
      catggggtg cacggtttc ggcggagga gcgggcggc 
      catcgcccg aacgaagca gattgacca gacgctcaa 
      agaggagag tcagagggc gaaaagaga tgttaaact 
      gttgctgtt aggcgccgg agaatcagg gaagagcac 
      gatagtcaa acaaatgaa aattatcca cgaaagtgg 
      gtttacaag cgaagactt caaacaata ccgacctgt 
      ggtgtatag caacactat tcaatccct tgtggccat 
      cctgcgggc catgccaaa cctcggaat cagctacgg 
      caacaacga gcgagagat cgatggtaa aatggtttt 
      cgacgttat ccaaaggat ggaagacac ggaaccttt 
      ttccgaaga actcctcgc cgccatgaa gcggttatg 
      ggctgattc gggggttca agaatgttt cggtcgttc 
      caacgaata ccaactgaa cgattccgc taaatattt 
      cctggacga tctggaccg attaggtgc caaagacta 
      tcaacctac ggaacagga catcctccg cacaagagt 
      caaaacgac ggggatcgt cgaagtgca tttctcgtt 
      taaaaacct caatttcaa attattcga tgtgggtgg 
      tcagaggtc agaaagaaa gaagtggat tcactgttt 
      cgaagacgt gacggcaat tattttctg cgtagcgat 
      gagcgaata tgatcaagt cctgcacga ggatgaaac 
      gacaaaccg aatgcagga gagcttgaa gcttttcga 
      ttcgatctg taacaacaa gtggttcac ggacacgtc 
      tataatcct cttccttaa taagaaaga cttgttcga 
      ggaaaaaat taggaagtc gcctttaac aatatgttt 
      ccccgaata cgcaggtgc gcaagagta tggcgaagc 
      agcggcgta catccaagc acaattcga agcgaagaa 
      caaatcgac aacaaaaga aatctactg ccatatgac 
      ctgtgccac agacacgca taatatcca gttcgtatt 
      cgacgcagt cactgatgt gatcatagc taataatct 
      gcgcaactg cggcctcta ctgagaaac ctctcatta 
      ctattcctt ttcatacat tattgactt tagtacatg 
      gttaaacca aaaaaatac aacagacat ggtagtgcc 
      ggtgtattt gctaaacgt tgcggtatt tctcggcca 
      ggggaagtc ccggcaacg tttgctgtt ataattgta 
      ccaaagttg agtgagatg tgttctttt ctgttgatc 
      gatgacctg gttggtgag ttgacacaa tacaagggg 
      tagctttcc tgtgcttga taataacat cggttgaca 
      aacagttta tttttctgt cgatttgtt tgacctaat 
      ctgtgcaca aaataattt gtatacaag atagtgtgc 
      cattaagaa gagattgca tttatcaac gtattgtag 
      ttgctttct ttttcttct aagttattg tttttttta 
      atatattac ggttcaatg ttatttatg tttgtataa 
      ttttaatga atgaaaaga gctatattg catagattt 
      agttaccaa atttgtaaa aactaagta tgtgttaat 
      gataatgtg gaaccaaat tattactgt ctggctcaa 
      gtagcgttc atctacaat atggtaatt aatattaat 
      tataataaa cattctaac tataaagcc tacttgtgt 
      gtaattagt ttcgtcaag tataatacc aaaacatat 
      ttttctaga ttttgttat tatacttta ctgtccgaa 
      ttgaatcag gaaattatg gacctttaa agaacagat 
      gcaaatata atataatac attaatgtg tacataaaa 
      actatatgg cgctttagc agatttgga gtagcttca 
      gtaccattt cgtagtgta gccagtgca tttactttg 
      atcatactg taatttaat gacatgcat tttaagtga 
      tacattcct ctctcgact attttgtat ctcccattt 
      acgcttttt ttacaagga aaccaacaa attgtaagt 
      gtatctaat tattcactc tctaattca ctaaagcag 
      acgttattt gaatacaat catccattt tagcgcaaa 
      actagtaat tgtcgtatc agtatatca aaagtaacg 
      taaaataca taactccga caagtgggc actaagcga 
      cctttgttc cttgtttag agtttcgcc aattttgtt 
      gtgtattct atgttaatt ttgcgcata caaccaaac 
      tgttatgta aattgtagt atttaatat ctggtagta 
      tcctattga gtcatgatt gaatctcta gtcagttta 
      ttttctaat tgtgactac ggcattata acttttttg 
      taatttatt tcaaaaata aaataagat gtaacccac 
      aattgtttt aatgcaggt ttatgttgt gcgacatct 
      acacagcta gagaaaaag tagtccaaa atggacaat 
      taaacttgt aggtagggt gcggacaat tttcttact 
      ttgaatgca caaaacgtg gacgaaaaa gttgctttc 
      ctattaatt attatccaa acgattttt cacacattt 
      ttgtacttg acgtctcgt ggatttaag acatatctt 
      tcaattggt cctcggttg ttttgtctt acttcgaca 
      cgctcataa cgctacaat tactattat tattcgaat 
      taaaccatg agactgtcc aagttttag ccacatgta 
      cacgcatac tattgactt gttgctttg ttgtgaata 
      agactaatt ataagaaga ttattaaag gtacggcgc 
      tagttaaat gcagtgaat gatgtacat gttttccaa 
      tatcagtaa atgtaacat tcataactg aggttgttc 
      tctcattgt atgtttgtt tctctgtga tgtgaatac 
      gataaataa attttaccc atttcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY