Status
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Gene information
General information
TC014520 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6382116..6385002
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014520
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
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      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_02297

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Fertility
      vitellogenic egg chamber latenot present - Number of animals showing the phenotype: 4
      ovary size decreased - Number of animals showing the phenotype: 4

      tropharium structure irregular - Number of animals showing the phenotype: 4
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      Pupal morphology
      wings shortened - Number of animals showing the phenotype: 6

      wings orientation irregular - Number of animals showing the phenotype: 6

      Adult morphology
      abdominal odoriferous glands content not present - Number of animals showing the phenotype: 4 (22 dpi)
      Days post injection



      Fertility
      egg production: number <50% of expected -
      egg chamber not present - Number of animals showing the phenotype: 3

      ovary without detectable defects - Number of animals showing the phenotype: 1
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014520 RA
        MAQAIKGAI ATHMRRLYQ GGPRLFCSF SRNNLPSEV 
        TTKYAQSTD FLLNNLKNA NSTKNVLDM VSQHNDIMN 
        NKHVIQALR SLFTLQKNG NSEMSTNEI LAHQDFRKL 
        CRKLRSQAA SIELGDIIE ALKVVSYVG VPADSTIFQ 
        VLLQLIRHN VNHLNLQQI IFLEFLLTQ TKSSPLVEA 
        LKIALPMVF EIHLPVKMD YDNAAHLAD CLYFASRNA 
        LNVESIEKL VNALMGHRE FDAKTAKSV VWSICDMEP 
        DEMFRPLMN RAIDSLIVH LDELSFNEM ETTLTKLIS 
        RFGQKCTYF YNETFYDSC ANYVIDKDL GFKQSIYIL 
        RKFGRINYH HKFMLDYMS KKVYENPEF ITKGDPLDI 
        YSIAVSTAL SEYRPVHWD TLKDLIIRS ENVANSAKK 
        EIIWIKFAA ALCLLDIYK LDVLTKCLN ENFLNALFN 
        KKRFKSDFE NYATIWQCI KLFKPELNC LLSTKFDPQ 
        VLVKHFRHN LDFPLEGAL HKALGGDKY VKTDLYSKM 
        GVEVDHVVV FRKGGYPVA MNYSEDLEF VEDIDVPSD 
        NQLVAILGL RHFGYTLNK KMRSSTNMA IRALEANGC 
        QVVPVCLEV WEGLPDFEK IPYLMQAIK ERTDSDLNV 
        TESVI
      CDS sequence
      • >TC014520 RA
        atggcacag gcaatcaag ggggcgatc gcgacgcac 
        atgagaagg ttataccaa gggggacct cgactcttt 
        tgcagtttt tcgagaaat aacctcccg tcagaagtc 
        accacgaaa tacgcccaa agcaccgat tttctcctc 
        aacaatctt aaaaatgcc aacagtacc aagaacgtc 
        ttggacatg gtctcgcaa cacaatgac atcatgaac 
        aacaaacac gttatccaa gctttacgt agcttattc 
        actttacaa aaaaacgga aattcagaa atgtccaca 
        aatgaaatt ctagcccac caagatttt cgcaaattg 
        tgtcgcaaa ttgcgctct caggctgcg agtattgag 
        ttgggtgat ataattgaa gcgttgaaa gttgtctcg 
        tatgttggg gttcccgcc gactcgact attttccaa 
        gttcttctt cagttgatc agacacaac gtgaatcat 
        ttaaatttg caacaaatt atctttctc gaatttttg 
        ttgactcag acaaagtct tctcctctg gtcgaggcg 
        ctaaaaata gccttgccc atggttttt gaaatacat 
        ttgccggtc aaaatggac tacgataat gccgcgcat 
        ttggctgat tgtttgtat ttcgcttcg cggaacgct 
        ttgaacgtc gaatcaatc gaaaaactc gtcaatgct 
        ttgatggga catcgcgaa tttgatgcg aaaaccgcc 
        aaaagtgta gtttggtca atttgcgat atggagccc 
        gatgagatg ttcaggccg ctaatgaat agagccatt 
        gattcactc attgtgcat ttagatgaa ttgagcttc 
        aacgaaatg gagactact ttgacgaaa ttaatcagt 
        cgatttggc cagaaatgc acgtacttt tacaacgag 
        acgttttac gactcgtgt gcgaactac gttattgac 
        aaggacttg ggtttcaaa cagtcgatt tatatttta 
        cgaaaattt ggacgcatt aattatcac cacaagttc 
        atgctggat tatatgtcg aaaaaagtg tacgaaaac 
        cccgaattt atcacaaaa ggggaccct ttggatatt 
        tacagcatt gctgtttcg accgctttg agcgaatat 
        cgacctgtt cattgggac acactaaaa gacctaatt 
        atacgaagt gagaatgtt gcaaattct gccaagaag 
        gaaataatt tggatcaaa ttcgctgcg gcgttatgt 
        cttctagac atctataaa ctggacgtt ctgacgaag 
        tgtttgaac gagaatttc ttaaatgcc ttgttcaat 
        aagaaaagg ttcaagtct gattttgaa aattacgcc 
        acgatttgg cagtgcatc aagttattt aaacctgaa 
        ctcaattgt ctgttgtca acaaagttt gacccacaa 
        gttctagtc aagcatttt agacacaat ttggatttt 
        cccttagaa ggggcttta cataaggcc ttaggaggg 
        gacaagtac gtcaagacg gacctttat tcgaaaatg 
        ggggtggaa gtcgatcat gtggtcgtg tttagaaaa 
        ggtggatat cctgtcgca atgaattat tcggaggat 
        ttagagttt gttgaagat attgacgtg ccgtccgac 
        aatcagtta gttgccatc ctaggttta cgacatttt 
        ggttatact ttgaacaag aaaatgagg agtagtaca 
        aatatggca atcagagcg ttggaggca aatgggtgt 
        caagtagtt cctgtgtgt ctagaagtg tgggaaggt 
        ttaccagac tttgaaaaa attccttat ttaatgcaa 
        gctattaag gaacggact gatagtgat ctgaatgtg 
        acagagagt gttatttaa
      mRNA sequence
      • >TC014520 RA
        agtttcgga aaatcgaaa ttgtttaaa aaaattata 
        aatgaaaaa tacacgatg gtgttaact aagaaaaag 
        tgatcattt cggggctta cttgttcaa acgtgaggt 
        ccataaggc gcatcctgg tctcatctc acgtgctag 
        aaccgactt gataaggtt agttttaat atcggcgta 
        cagttgcgt cgattcctc gtccaattt ctcggcata 
        gggcagtaa tttgttgta aacattctc cttttgggg 
        tttaaagcc cagtttttc gtaacgtct tttgctaac 
        attttcatc catgagcgt gcgattcgc gcaaacctc 
        gaacctatc acacacact ttaacaacc acaacgata 
        gattaacaa tgcacgatt gtttaacaa cttgctatt 
        tttattgtt ttgtactag tcatttgca gacatattt 
        gtttcatac aaagtggca ctttttcaa ccaatccgg 
        ccgattttt ccaaagtgc catctcatg ccaaccaat 
        cgaagcaat gcgcgccaa gcgccaaat tcaaaccgg 
        cgaggaagg acgtttatt taaatacga gggcgatca 
        gccaataaa tcgcctatt ttaagttgc agggcaatg 
        gcacaggca atcaagggg gcgatcgcg acgcacatg 
        agaaggtta taccaaggg ggacctcga ctcttttgc 
        agtttttcg agaaataac ctcccgtca gaagtcacc 
        acgaaatac gcccaaagc accgatttt ctcctcaac 
        aatcttaaa aatgccaac agtaccaag aacgtcttg 
        gacatggtc tcgcaacac aatgacatc atgaacaac 
        aaacacgtt atccaagct ttacgtagc ttattcact 
        ttacaaaaa aacggaaat tcagaaatg tccacaaat 
        gaaattcta gcccaccaa gattttcgc aaattgtgt 
        cgcaaattg cgctctcag gctgcgagt attgagttg 
        ggtgatata attgaagcg ttgaaagtt gtctcgtat 
        gttggggtt cccgccgac tcgactatt ttccaagtt 
        cttcttcag ttgatcaga cacaacgtg aatcattta 
        aatttgcaa caaattatc tttctcgaa tttttgttg 
        actcagaca aagtcttct cctctggtc gaggcgcta 
        aaaatagcc ttgcccatg gtttttgaa atacatttg 
        ccggtcaaa atggactac gataatgcc gcgcatttg 
        gctgattgt ttgtatttc gcttcgcgg aacgctttg 
        aacgtcgaa tcaatcgaa aaactcgtc aatgctttg 
        atgggacat cgcgaattt gatgcgaaa accgccaaa 
        agtgtagtt tggtcaatt tgcgatatg gagcccgat 
        gagatgttc aggccgcta atgaataga gccattgat 
        tcactcatt gtgcattta gatgaattg agcttcaac 
        gaaatggag actactttg acgaaatta atcagtcga 
        tttggccag aaatgcacg tacttttac aacgagacg 
        ttttacgac tcgtgtgcg aactacgtt attgacaag 
        gacttgggt ttcaaacag tcgatttat attttacga 
        aaatttgga cgcattaat tatcaccac aagttcatg 
        ctggattat atgtcgaaa aaagtgtac gaaaacccc 
        gaatttatc acaaaaggg gaccctttg gatatttac 
        agcattgct gtttcgacc gctttgagc gaatatcga 
        cctgttcat tgggacaca ctaaaagac ctaattata 
        cgaagtgag aatgttgca aattctgcc aagaaggaa 
        ataatttgg atcaaattc gctgcggcg ttatgtctt 
        ctagacatc tataaactg gacgttctg acgaagtgt 
        ttgaacgag aatttctta aatgccttg ttcaataag 
        aaaaggttc aagtctgat tttgaaaat tacgccacg 
        atttggcag tgcatcaag ttatttaaa cctgaactc 
        aattgtctg ttgtcaaca aagtttgac ccacaagtt 
        ctagtcaag cattttaga cacaatttg gattttccc 
        ttagaaggg gctttacat aaggcctta ggaggggac 
        aagtacgtc aagacggac ctttattcg aaaatgggg 
        gtggaagtc gatcatgtg gtcgtgttt agaaaaggt 
        ggatatcct gtcgcaatg aattattcg gaggattta 
        gagtttgtt gaagatatt gacgtgccg tccgacaat 
        cagttagtt gccatccta ggtttacga cattttggt 
        tatactttg aacaagaaa atgaggagt agtacaaat 
        atggcaatc agagcgttg gaggcaaat gggtgtcaa 
        gtagttcct gtgtgtcta gaagtgtgg gaaggttta 
        ccagacttt gaaaaaatt ccttattta atgcaagct 
        attaaggaa cggactgat agtgatctg aatgtgaca 
        gagagtgtt atttaaaaa caataaaat tttatatta 
        gaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY