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Gene information
General information
TC014514 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 4336921..4338132
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014514
    iBeetle screen iB_02296
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014514 RA
      MMLTLPFRN FIKKAANRT LQNSLLLRA KSTKEEKSE 
      AEKSQEAAL EEKLFLVDE NDKVIGHAS KRECHQVKK 
      DGEIPLHRA FSVFLFSSH GDLLLQRRS EEKITYPGL 
      YTNSCCSHP IVDIEGEGE ECDALGVKK AAQRRLGFE 
      LGIPKDKLP LEKFNYITR VHYKDEGNG EWGEHEIDY 
      VLFFQGDVP VNPNPNEIS EISYVPRHE LDQHLPTLG 
      TLTPWFHLI LKHRLKLWW DNLDKIDKI IDHEKILKL 
      GFPK
    CDS sequence
    • >TC014514 RA
      atgatgctg actctcccc tttagaaat tttataaag 
      aaagctgca aatcgcact ttgcaaaac agtctttta 
      ctgcgggcc aagtcgacc aaggaagag aaaagtgag 
      gctgagaag agccaagaa gctgctctc gaagagaaa 
      ctttttctg gttgatgag aatgataaa gttataggg 
      catgcctct aagagggag tgccaccaa gtgaagaaa 
      gacggcgaa attccgctg catagggcc tttagtgtg 
      tttctgttt agcagccat ggagatttg ctgttgcaa 
      aggcgctct gaagaaaaa atcacatac cctggactg 
      tacacgaat tcgtgttgt agtcacccc atcgtggac 
      attgaaggg gagggagag gagtgcgac gctttgggg 
      gtgaaaaag gcggctcag aggaggtta gggtttgag 
      ttggggatt cccaaagac aagttacct ctagagaag 
      tttaattat atcacgaga gtgcactat aaggacgaa 
      gggaatgga gaatggggc gaacacgag attgattac 
      gtcttgttc ttccaaggt gatgtccct gtgaatccg 
      aatccgaac gaaatctca gaaattagc tacgtcccg 
      agacacgaa ttagaccag catttacca accctaggg 
      accctaaca ccctggttt cacctaatc ctcaagcac 
      agactcaaa ctttggtgg gataattta gacaaaata 
      gataaaatc atcgatcac gagaaaatc ctcaagtta 
      ggattcccg aagtaa
    mRNA sequence
    • >TC014514 RA
      tatcacctc cgacactgg gcagaattg tattgttag 
      ttgcataac ggttgatgc ttcgataat ttgacattt 
      gtcattctg tggtccaat ttaaccaat tttcttggg 
      caaaaactc gtaaaatta tcgacattg aaacaattt 
      acaagtgcg gcttaaaac cagtgtaat taaagtgtt 
      atgtgtttg ctgagtgaa ccaaaaaaa ccataaaaa 
      gctctcttg tgtgttggt tataaatca tgatgctga 
      ctctcccct ttagaaatt ttataaaga aagctgcaa 
      atcgcactt tgcaaaaca gtcttttac tgcgggcca 
      agtcgacca aggaagaga aaagtgagg ctgagaaga 
      gccaagaag ctgctctcg aagagaaac tttttctgg 
      ttgatgaga atgataaag ttatagggc atgcctcta 
      agagggagt gccaccaag tgaagaaag acggcgaaa 
      ttccgctgc atagggcct ttagtgtgt ttctgttta 
      gcagccatg gagatttgc tgttgcaaa ggcgctctg 
      aagaaaaaa tcacatacc ctggactgt acacgaatt 
      cgtgttgta gtcacccca tcgtggaca ttgaagggg 
      agggagagg agtgcgacg ctttggggg tgaaaaagg 
      cggctcaga ggaggttag ggtttgagt tggggattc 
      ccaaagaca agttacctc tagagaagt ttaattata 
      tcacgagag tgcactata aggacgaag ggaatggag 
      aatggggcg aacacgaga ttgattacg tcttgttct 
      tccaaggtg atgtccctg tgaatccga atccgaacg 
      aaatctcag aaattagct acgtcccga gacacgaat 
      tagaccagc atttaccaa ccctaggga ccctaacac 
      cctggtttc acctaatcc tcaagcaca gactcaaac 
      tttggtggg ataatttag acaaaatag ataaaatca 
      tcgatcacg agaaaatcc tcaagttag gattcccga 
      agtaaattt aactactta taaagggcc ttaatctaa 
      gtggaaagt tgattaaaa agcggttgt tttattggc 
      ttatattct attaatctg tcccaattt aatttttta 
      taatgtaaa tatataaca agggtcaac aataaagct 
      tgttaaaat at