Status
Please wait ...
Gene information
General information
TC014506 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6522883..6527840
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014506
    iBeetle screen iB_02294

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014506 RA
      MTSIIKLQA LSGAMDESP PCYILQVDE VRILLDCGW 
      DEHFDMEII KEMRRHVHT IDAVLISYP DVAHLGALP 
      YLVGKLGLN CPIYATIPV YKMGQMFMY DLFQSHYNM 
      EDFDLFTLD DVDATFEKV IQLKYNQSV PLKGKGYGL 
      TITPLPAGH MIGGTIWKI MKVGEEDII YANDFNHKK 
      ERHLNGCEL EKLQRPSLF ITDAFNATY QQARRRARD 
      EKLMTNILQ TLRNNGNVL VAVDTAGRV LELAHMLDQ 
      LWRNKESGL LVYSLALLS NVSYNVVEF AKSQIEWMS 
      DKLMRSFEG ARNNPFQFK HLQLCHSLH ELQKVSSPK 
      VVLASSPDM ESGFSRELF LQWCSNPNN SIIITTRTS 
      PGTLARDLV DNGGNRQID LVVKRRVKL EGSELEEYQ 
      KSQREKREE NSSRDEESD SDDDIEMSV ISKGRHDIV 
      IKQEGKTSG GFFKVTKKQ YPIYPFHEE KIKCDEYGE 
      IIKPEDYKL ADVVTETED NKENVVIKK EEEVIPEVA 
      ETPSKCIVL SRTVQVNCQ VQYIDFEGR SDGESLMKI 
      LSQLRPRRV IIVRGSPES TNTIKNHCQ ENLDARVFA 
      PVRGEVVDA TTETHIYQV RLTDALVSQ LNFQKAKDA 
      EVAWLNAQI VVRESQLDA RRMNVDNEP MEVDEEESK 
      ILTLEPYGD NIPHDTVFI NELKLSEFK QILAKSNIN 
      SEFSGGVLW CSNGTLAIR RVETGRVIL EGCISEDYY 
      KVKELLYEQ YAVL
    CDS sequence
    • >TC014506 RA
      atgacttca ataatcaaa ctgcaggcc ctgtcgggg 
      gcaatggac gagtcgccc ccttgctac atcctgcaa 
      gtcgacgaa gtgcgtatt ttactggac tgtggatgg 
      gacgaacat ttcgatatg gaaattatc aaagaaatg 
      agaaggcat gttcacacg attgacgcc gtcttgatt 
      tcgtaccct gatgtggcg catttgggg gctcttcca 
      tatttagtc ggaaagttg ggcttgaat tgccctatt 
      tatgccaca ataccggtc tataagatg gggcaaatg 
      tttatgtat gacttgttt cagtctcat tataatatg 
      gaagacttt gatttattc acattggat gatgtggac 
      gccactttt gagaaagtt attcagctg aagtataat 
      cagagtgtt ccattaaaa ggcaagggg tatggattg 
      acaatcacg cccttgcct gctgggcac atgattggg 
      ggcacaatt tggaaaata atgaaggtt ggtgaagaa 
      gacattatt tacgctaat gatttcaac cacaaaaaa 
      gagaggcat ttgaacggt tgtgagtta gaaaagtta 
      caaaggccc tctctgttc atcactgat gcgtttaat 
      gctacgtac caacaagcc aggagaagg gctcgggac 
      gaaaaacta atgaccaac attttgcaa actttgagg 
      aacaacggc aacgttctg gtcgctgtg gacacagca 
      ggccgtgtt ttagaactg gcccatatg ttggaccag 
      ctttggaga aacaaggaa tcaggcctt ctagtctat 
      tctctagct ctgctaagc aatgtcagt tacaacgtt 
      gtagagttt gcaaagtct caaattgag tggatgagt 
      gataaactg atgaggagt tttgaaggc gcgagaaat 
      aatcctttc cagttcaaa catctgcaa ctgtgtcac 
      agtttacac gaactacag aaggtttca agtcctaaa 
      gttgttctc gcgagttcg cctgatatg gagagtggg 
      ttctctcgc gagttgttt cttcagtgg tgctcaaat 
      cctaataat agtataatt atcacgact aggacgtct 
      ccggggaca ttagctcga gatttagtc gataatggg 
      ggcaatcgc caaatcgat ttggttgtg aaaagacga 
      gtcaagtta gaaggtagc gaacttgaa gagtaccaa 
      aagagccaa agagaaaaa cgagaggaa aattccagc 
      agggacgag gagtcggat tcagacgat gatattgaa 
      atgagtgtc atatcaaaa gggcggcac gatattgtc 
      ataaaacaa gaaggcaag acctcaggt ggctttttc 
      aaagttacg aaaaaacaa tatcccatc taccctttt 
      cacgaagaa aaaatcaaa tgtgacgaa tacggcgaa 
      ataatcaaa ccggaagac tacaagcta gctgatgtc 
      gtcacagaa actgaagat aataaggaa aatgttgtg 
      atcaaaaaa gaagaagag gtcattcct gaggtggct 
      gagacgccg tccaagtgc atcgtactt agcaggact 
      gtccaggtt aactgtcaa gtgcaatat attgacttc 
      gaaggtcgc tcagacggg gaatccctc atgaaaatt 
      ttgtctcag ttgaggccc agaagagtt ataatcgtc 
      cgaggttcg cctgagagt actaacacg ataaaaaat 
      cactgtcag gagaatctc gatgcaaga gtgtttgct 
      ccagtcagg ggtgaagtc gtggatgcg acaactgag 
      actcatatt tatcaagtg cgtctgact gatgctctg 
      gtgtctcaa ctgaatttc cagaaggct aaagatgcc 
      gaagttgcg tggcttaac gcccagatt gtggtcagg 
      gaaagtcag ttggatgcg aggaggatg aatgtcgac 
      aacgaacca atggaagtc gatgaggaa gagtcaaaa 
      atattgact ctggagccc tacggcgac aacatcccc 
      cacgacaca gtattcatc aacgaacta aaactttcc 
      gaattcaaa caaatcctg gctaaaagc aacatcaat 
      tcagaattt tcaggcgga gttttgtgg tgcagcaac 
      ggcacttta gccatcagg agggtagaa acagggaga 
      gtgatcctc gaaggttgc atttcggaa gattattac 
      aaagtgaaa gagcttttg tatgagcag tacgcagta 
      ctgtag
    mRNA sequence
    • >TC014506 RA
      caccaaaac tgtccaaaa tatgagtta attttgcac 
      atttgtgta ttaaattaa cttctggtc acaaaacac 
      ccaatatgc tcaccgttt aataagttg gggcacacc 
      acataaaat gacttcaat aatcaaact gcaggccct 
      gtcgggggc aatggacga gtcgccccc ttgctacat 
      cctgcaagt cgacgaagt gcgtatttt actggactg 
      tggatggga cgaacattt cgatatgga aattatcaa 
      agaaatgag aaggcatgt tcacacgat tgacgccgt 
      cttgatttc gtaccctga tgtggcgca tttgggggc 
      tcttccata tttagtcgg aaagttggg cttgaattg 
      ccctattta tgccacaat accggtcta taagatggg 
      gcaaatgtt tatgtatga cttgtttca gtctcatta 
      taatatgga agactttga tttattcac attggatga 
      tgtggacgc cacttttga gaaagttat tcagctgaa 
      gtataatca gagtgttcc attaaaagg caaggggta 
      tggattgac aatcacgcc cttgcctgc tgggcacat 
      gattggggg cacaatttg gaaaataat gaaggttgg 
      tgaagaaga cattattta cgctaatga tttcaacca 
      caaaaaaga gaggcattt gaacggttg tgagttaga 
      aaagttaca aaggccctc tctgttcat cactgatgc 
      gtttaatgc tacgtacca acaagccag gagaagggc 
      tcgggacga aaaactaat gaccaacat tttgcaaac 
      tttgaggaa caacggcaa cgttctggt cgctgtgga 
      cacagcagg ccgtgtttt agaactggc ccatatgtt 
      ggaccagct ttggagaaa caaggaatc aggccttct 
      agtctattc tctagctct gctaagcaa tgtcagtta 
      caacgttgt agagtttgc aaagtctca aattgagtg 
      gatgagtga taaactgat gaggagttt tgaaggcgc 
      gagaaataa tcctttcca gttcaaaca tctgcaact 
      gtgtcacag tttacacga actacagaa ggtttcaag 
      tcctaaagt tgttctcgc gagttcgcc tgatatgga 
      gagtgggtt ctctcgcga gttgtttct tcagtggtg 
      ctcaaatcc taataatag tataattat cacgactag 
      gacgtctcc ggggacatt agctcgaga tttagtcga 
      taatggggg caatcgcca aatcgattt ggttgtgaa 
      aagacgagt caagttaga aggtagcga acttgaaga 
      gtaccaaaa gagccaaag agaaaaacg agaggaaaa 
      ttccagcag ggacgagga gtcggattc agacgatga 
      tattgaaat gagtgtcat atcaaaagg gcggcacga 
      tattgtcat aaaacaaga aggcaagac ctcaggtgg 
      ctttttcaa agttacgaa aaaacaata tcccatcta 
      cccttttca cgaagaaaa aatcaaatg tgacgaata 
      cggcgaaat aatcaaacc ggaagacta caagctagc 
      tgatgtcgt cacagaaac tgaagataa taaggaaaa 
      tgttgtgat caaaaaaga agaagaggt cattcctga 
      ggtggctga gacgccgtc caagtgcat cgtacttag 
      caggactgt ccaggttaa ctgtcaagt gcaatatat 
      tgacttcga aggtcgctc agacgggga atccctcat 
      gaaaatttt gtctcagtt gaggcccag aagagttat 
      aatcgtccg aggttcgcc tgagagtac taacacgat 
      aaaaaatca ctgtcagga gaatctcga tgcaagagt 
      gtttgctcc agtcagggg tgaagtcgt ggatgcgac 
      aactgagac tcatattta tcaagtgcg tctgactga 
      tgctctggt gtctcaact gaatttcca gaaggctaa 
      agatgccga agttgcgtg gcttaacgc ccagattgt 
      ggtcaggga aagtcagtt ggatgcgag gaggatgaa 
      tgtcgacaa cgaaccaat ggaagtcga tgaggaaga 
      gtcaaaaat attgactct ggagcccta cggcgacaa 
      catccccca cgacacagt attcatcaa cgaactaaa 
      actttccga attcaaaca aatcctggc taaaagcaa 
      catcaattc agaattttc aggcggagt tttgtggtg 
      cagcaacgg cactttagc catcaggag ggtagaaac 
      agggagagt gatcctcga aggttgcat ttcggaaga 
      ttattacaa agtgaaaga gcttttgta tgagcagta 
      cgcagtact gtagtgttt ttcataaat tttcgtacc 
      tttttgttt tgttttttt ttccttctg attcatccc 
      ctcccccac gcggtggcg tcaagtgag ttaagaata 
      tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY