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Gene information
General information
TC014483 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10980074..10983869
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014483
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02291

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014483 RA
      MDEIKLDLP QIKQDLLHG IVACSQRGL NHSAKWLSE 
      LHYSLAYIK STPEENVQF KDDCEGELE AYFMAKSYF 
      DLKEYDRCA HFLKKCTKP KTRFLYLYA RYFSIQKKK 
      LDSMTDTNC PPDPSENGD LTELCRELK SDYYENKLD 
      GFCLYLYGI ILKQLDMIS LAITVFVKS VNLEPLLWC 
      SWYELGKII PDKSKIFSV QLPDHWMKH FFLAHAYLE 
      QLNNDEALQ IYFELYSQG LKNSTYLMA QIAIGHHNR 
      RELFHAIDL FKEILSVDP YRLDNLDTY SNLLYVQEM 
      KTELADLAH KVVLIDKYR VETCCVIGN YYSLRSDHA 
      KAVLYFRRA LKLNPQFLS AWTLMGHEY MEMKNTNAA 
      IQSYRHAIE INNRDYRAW YGLGQTYEI LKMYFYCLY 
      YYKQAQQLK PNDSRMIIA LGETYEKLE KTENALKCY 
      YKACKVGDI EGQALIKLA KLYDKLKDE DNAAAAFTE 
      FCLRDDENK GRFNEDQTE FYSALQYLA NYFLKRGDL 
      DQAKIYANK CLENEKAKE VAKALLKEI AVKRLLTES 
      LGSDTVPMD GTCSNILPP DDDGGSDMD ITNTSTK
    • >TC014483 RB
      MDEIKLDLP QIKQDLLHG IVACSQRGL NHSAKWLSE 
      LHYSLAYIK STPEENVQF KDDCEGELE AYFMAKSYF 
      DLKEYDRCA HFLKKCTKP KTRFLYLYA RYFSIQKKK 
      LDSMTDTNC PPDPSENGD LTELCRELK SDYYENKLD 
      GFCLYLYGI ILKQLDMIS LAITVFVKS VNLEPLLWC 
      SWYELGKII PDKSKIFSV QLPDHWMKH FFLAHAYLE 
      QLNNDEALQ IYFELYSQG LKNSTYLMA QIAIGHHNR 
      RAKLLNVRH SPVIHVFAL EQNGPHNRQ NQVSDSLDL 
      LRLVTGVPQ VNRFFQNHA NATAQNRDK GHKASPDCL 
      LPETTQAFE APIHRSEGL PGVLHRPQV NLNHARFHL 
      VDAVTVRAT NSCHNLRRK VPQSPVVVI AVFANFPRN 
      DTGNDGREQ QMEVLLNVN VGVGHQAFD HSHRINQKR 
      QVSLRTCSL NPKAPSVNL TKKTCSSAD RGWGSGCSL 
      SKIGSGFGW NFLAGGWGT AGGFFCSWY SSRSFKYSD 
      IVAELFHAI DLFKEILSV DPYRLDNLD TYSNLLYVQ 
      EMKTELADL AHKVVLIDK YRVETCCVI GNYYSLRSD 
      HAKAVLYFR RALKLNPQF LSAWTLMGH EYMEMKNTN 
      AAIQSYRHA IEINNRDYR AWYGLGQTY EILKMYFYC 
      LYYYKQAQQ LKPNDSRMI IALGETYEK LEKTENALK 
      CYYKACKVG DIEGQALIK LAKLYDKLK DEDNAAAAF 
      TEFCLRDDE NKGRFNEDQ TEFYSALQY LANYFLKRG 
      DLDQAKIYA NKCLENEKA KEVAKALLK EIAVKRLLT 
      ESLGSDTVP MDGTCSNIL PPDDDGGSD MDITNTSTK
    CDS sequence
    • >TC014483 RA
      atggatgaa atcaagcta gatttgccc caaatcaag 
      caagacttg ctgcacggg attgttgca tgctcacaa 
      cggggtcta aatcacagt gcaaagtgg ttatccgaa 
      cttcactac tccctagcg tacataaaa tccactccc 
      gaggaaaat gttcagttt aaagatgat tgcgagggc 
      gaattggaa gcatatttc atggcgaag agctacttt 
      gacttgaaa gagtacgac cggtgtgcc catttcctg 
      aaaaagtgc accaaaccc aagacacgt tttttgtac 
      ctctacgcc cggtatttc tcaattcag aagaaaaaa 
      ttagacagt atgaccgac acgaattgt ccacccgat 
      ccctcggaa aacggcgac ttgaccgaa ttatgtcgg 
      gaattaaaa agcgactat tacgagaat aaattggac 
      gggttctgc ttgtacttg tacgggatt attctgaag 
      caactggac atgatttcg ttggcaatc accgttttt 
      gtcaagtcg gtgaatctg gagcccctc ttgtggtgc 
      tcttggtac gaactcggt aaaataatt cccgacaaa 
      agcaagatt ttctcagtc cagcttccc gaccactgg 
      atgaaacac ttcttcctg gcacacgca tacctcgag 
      caactgaac aacgacgaa gctttacaa atttatttc 
      gaactgtac tcacaaggg ctcaaaaac agcacctat 
      ctaatggcc cagatcgca atcggccac cacaaccgg 
      cgagaacta ttccacgcg attgacttg tttaaggag 
      attttaagt gtggatcct tacagacta gataatttg 
      gatacttac tcgaatctt ttgtacgtg caagaaatg 
      aagactgag ttggccgat ttggcgcac aaggtggtg 
      ctgattgac aagtacagg gtggagacg tgttgcgtg 
      attgggaat tactacagt ctgaggtca gatcatgcc 
      aaggctgta ctgtacttt cgcagggct ttgaagctc 
      aatccccag tttttgtca gcgtggacg ctaatgggg 
      cacgagtac atggaaatg aaaaacaca aatgcggcg 
      attcagagc tacagacac gcaattgaa attaataat 
      cgggattac cgggcttgg tacggcctc ggccaaacc 
      tacgagatc ctcaaaatg tacttctat tgcctctac 
      tattacaag caagcccaa caattaaaa cccaacgac 
      agtcgcatg attatcgcc cttggcgag acctacgaa 
      aagttagaa aagaccgaa aatgccctc aagtgttac 
      tacaaggcg tgcaaagtt ggggacatt gaaggccaa 
      gctttgatc aaactcgca aagctttac gataaatta 
      aaagacgaa gataacgcc gctgccgcg tttacagag 
      ttttgcttg cgtgacgat gagaacaag ggacggttc 
      aatgaagac caaactgag ttctacagc gctctccaa 
      tatctagcg aattatttt ttaaagagg ggagatttg 
      gaccaagca aagatttac gccaacaag tgcctggag 
      aatgagaag gcgaaagaa gttgcgaag gccttgttg 
      aaagaaatc gctgtaaaa agactacta accgaaagc 
      ttgggttca gacactgtt ccaatggat ggaacctgt 
      agtaatatt ttgcccccg gacgacgat ggaggctct 
      gatatggac attactaat acgtccacc aaataa
    • >TC014483 RB
      atggatgaa atcaagcta gatttgccc caaatcaag 
      caagacttg ctgcacggg attgttgca tgctcacaa 
      cggggtcta aatcacagt gcaaagtgg ttatccgaa 
      cttcactac tccctagcg tacataaaa tccactccc 
      gaggaaaat gttcagttt aaagatgat tgcgagggc 
      gaattggaa gcatatttc atggcgaag agctacttt 
      gacttgaaa gagtacgac cggtgtgcc catttcctg 
      aaaaagtgc accaaaccc aagacacgt tttttgtac 
      ctctacgcc cggtatttc tcaattcag aagaaaaaa 
      ttagacagt atgaccgac acgaattgt ccacccgat 
      ccctcggaa aacggcgac ttgaccgaa ttatgtcgg 
      gaattaaaa agcgactat tacgagaat aaattggac 
      gggttctgc ttgtacttg tacgggatt attctgaag 
      caactggac atgatttcg ttggcaatc accgttttt 
      gtcaagtcg gtgaatctg gagcccctc ttgtggtgc 
      tcttggtac gaactcggt aaaataatt cccgacaaa 
      agcaagatt ttctcagtc cagcttccc gaccactgg 
      atgaaacac ttcttcctg gcacacgca tacctcgag 
      caactgaac aacgacgaa gctttacaa atttatttc 
      gaactgtac tcacaaggg ctcaaaaac agcacctat 
      ctaatggcc cagatcgca atcggccac cacaaccgg 
      cgagccaaa ctccttaac gtaagacac tcccccgtc 
      attcacgtt tttgcattg gagcaaaac gggccccac 
      aaccgccag aaccaagtc agtgattcg cttgatttg 
      cttcgtctc gtgactggg gtcccccaa gtaaaccgt 
      tttttccaa aaccatgca aacgcgacg gctcaaaac 
      cgggataag gggcacaag gcaagtcct gactgtctc 
      ctccccgaa acaactcag gcctttgag gcccccata 
      caccgtagt gaggggctt ccgggggtc ttgcaccgc 
      ccgcaagta aaccttaat cacgcgcgt tttcatctg 
      gttgatgct gttactgtg cgtgcaaca aatagttgc 
      cacaacttg cgccgaaaa gtccctcaa agtccagtg 
      gttgtcata gctgtattt gctaatttt ccgcgaaac 
      gacacagga aatgacggc cgggagcag caaatggag 
      gtgctcctt aatgttaac gtgggggtt ggccatcag 
      gcttttgac cactcgcac cgaataaat caaaagaga 
      caagtttcg ttgcggacg tgctcgttg aacccgaaa 
      gccccctct gtcaattta acgaaaaaa acctgctct 
      tcggcggat agggggtgg gggtcaggg tgcagtttg 
      agtaaaatt gggtcaggt tttggctgg aatttcttg 
      gccgggggt tggggtaca gcaggcggg tttttttgt 
      tcctggtac agttccagg tcttttaaa tattccgac 
      attgttgca gaactattc cacgcgatt gacttgttt 
      aaggagatt ttaagtgtg gatccttac agactagat 
      aatttggat acttactcg aatcttttg tacgtgcaa 
      gaaatgaag actgagttg gccgatttg gcgcacaag 
      gtggtgctg attgacaag tacagggtg gagacgtgt 
      tgcgtgatt gggaattac tacagtctg aggtcagat 
      catgccaag gctgtactg tactttcgc agggctttg 
      aagctcaat ccccagttt ttgtcagcg tggacgcta 
      atggggcac gagtacatg gaaatgaaa aacacaaat 
      gcggcgatt cagagctac agacacgca attgaaatt 
      aataatcgg gattaccgg gcttggtac ggcctcggc 
      caaacctac gagatcctc aaaatgtac ttctattgc 
      ctctactat tacaagcaa gcccaacaa ttaaaaccc 
      aacgacagt cgcatgatt atcgccctt ggcgagacc 
      tacgaaaag ttagaaaag accgaaaat gccctcaag 
      tgttactac aaggcgtgc aaagttggg gacattgaa 
      ggccaagct ttgatcaaa ctcgcaaag ctttacgat 
      aaattaaaa gacgaagat aacgccgct gccgcgttt 
      acagagttt tgcttgcgt gacgatgag aacaaggga 
      cggttcaat gaagaccaa actgagttc tacagcgct 
      ctccaatat ctagcgaat tatttttta aagagggga 
      gatttggac caagcaaag atttacgcc aacaagtgc 
      ctggagaat gagaaggcg aaagaagtt gcgaaggcc 
      ttgttgaaa gaaatcgct gtaaaaaga ctactaacc 
      gaaagcttg ggttcagac actgttcca atggatgga 
      acctgtagt aatattttg cccccggac gacgatgga 
      ggctctgat atggacatt actaatacg tccaccaaa 
      taa
    mRNA sequence
    • >TC014483 RA
      atctaactg acctaacct cacctaacc tcgaaaatt 
      tgaattttt aatttgtgt aaagtgttt tttcgcgat 
      agatggatg aaatcaagc tagatttgc cccaaatca 
      agcaagact tgctgcacg ggattgttg catgctcac 
      aacggggtc taaatcaca gtgcaaagt ggttatccg 
      aacttcact actccctag cgtacataa aatccactc 
      ccgaggaaa atgttcagt ttaaagatg attgcgagg 
      gcgaattgg aagcatatt tcatggcga agagctact 
      ttgacttga aagagtacg accggtgtg cccatttcc 
      tgaaaaagt gcaccaaac ccaagacac gttttttgt 
      acctctacg cccggtatt tctcaattc agaagaaaa 
      aattagaca gtatgaccg acacgaatt gtccacccg 
      atccctcgg aaaacggcg acttgaccg aattatgtc 
      gggaattaa aaagcgact attacgaga ataaattgg 
      acgggttct gcttgtact tgtacggga ttattctga 
      agcaactgg acatgattt cgttggcaa tcaccgttt 
      ttgtcaagt cggtgaatc tggagcccc tcttgtggt 
      gctcttggt acgaactcg gtaaaataa ttcccgaca 
      aaagcaaga ttttctcag tccagcttc ccgaccact 
      ggatgaaac acttcttcc tggcacacg catacctcg 
      agcaactga acaacgacg aagctttac aaatttatt 
      tcgaactgt actcacaag ggctcaaaa acagcacct 
      atctaatgg cccagatcg caatcggcc accacaacc 
      ggcgagaac tattccacg cgattgact tgtttaagg 
      agattttaa gtgtggatc cttacagac tagataatt 
      tggatactt actcgaatc ttttgtacg tgcaagaaa 
      tgaagactg agttggccg atttggcgc acaaggtgg 
      tgctgattg acaagtaca gggtggaga cgtgttgcg 
      tgattggga attactaca gtctgaggt cagatcatg 
      ccaaggctg tactgtact ttcgcaggg ctttgaagc 
      tcaatcccc agtttttgt cagcgtgga cgctaatgg 
      ggcacgagt acatggaaa tgaaaaaca caaatgcgg 
      cgattcaga gctacagac acgcaattg aaattaata 
      atcgggatt accgggctt ggtacggcc tcggccaaa 
      cctacgaga tcctcaaaa tgtacttct attgcctct 
      actattaca agcaagccc aacaattaa aacccaacg 
      acagtcgca tgattatcg cccttggcg agacctacg 
      aaaagttag aaaagaccg aaaatgccc tcaagtgtt 
      actacaagg cgtgcaaag ttggggaca ttgaaggcc 
      aagctttga tcaaactcg caaagcttt acgataaat 
      taaaagacg aagataacg ccgctgccg cgtttacag 
      agttttgct tgcgtgacg atgagaaca agggacggt 
      tcaatgaag accaaactg agttctaca gcgctctcc 
      aatatctag cgaattatt ttttaaaga ggggagatt 
      tggaccaag caaagattt acgccaaca agtgcctgg 
      agaatgaga aggcgaaag aagttgcga aggccttgt 
      tgaaagaaa tcgctgtaa aaagactac taaccgaaa 
      gcttgggtt cagacactg ttccaatgg atggaacct 
      gtagtaata ttttgcccc cggacgacg atggaggct 
      ctgatatgg acattacta atacgtcca ccaaataat 
      atattgtgt acagattaa ctatcaata aaaatacca 
      tttctcata aaacgactt ttatttaca acctgtaca 
      actttgcgt aataaaata aaaggcata agcagtcaa 
      aaaaattaa aattgcgta accccctcg aaggaattt 
      gtgttcttc aactccgct tgttcaaat ctggcaata 
      agtccgtta acgaaagcg tcaaagcgt tccaaattt 
      gggttgttt ggaattaaa tgtccactg atgatttct 
      ttgaattct cgtctagtg ccacgaatg cgaattttt 
      ctccaattg ggtgaagag tgcagattt atcacgaat 
      ttgtgatac ctgctgtaa cattcacaa gacgatgaa 
      actgtgatc ctttcctcc tcggggact ggagacgaa 
      gttctcggg aacgacatg gtggtgaaa attctataa 
      caattaaaa aagatatga gtta
    • >TC014483 RB
      atctaactg acctaacct cacctaacc tcgaaaatt 
      tgaattttt aatttgtgt aaagtgttt tttcgcgat 
      agatggatg aaatcaagc tagatttgc cccaaatca 
      agcaagact tgctgcacg ggattgttg catgctcac 
      aacggggtc taaatcaca gtgcaaagt ggttatccg 
      aacttcact actccctag cgtacataa aatccactc 
      ccgaggaaa atgttcagt ttaaagatg attgcgagg 
      gcgaattgg aagcatatt tcatggcga agagctact 
      ttgacttga aagagtacg accggtgtg cccatttcc 
      tgaaaaagt gcaccaaac ccaagacac gttttttgt 
      acctctacg cccggtatt tctcaattc agaagaaaa 
      aattagaca gtatgaccg acacgaatt gtccacccg 
      atccctcgg aaaacggcg acttgaccg aattatgtc 
      gggaattaa aaagcgact attacgaga ataaattgg 
      acgggttct gcttgtact tgtacggga ttattctga 
      agcaactgg acatgattt cgttggcaa tcaccgttt 
      ttgtcaagt cggtgaatc tggagcccc tcttgtggt 
      gctcttggt acgaactcg gtaaaataa ttcccgaca 
      aaagcaaga ttttctcag tccagcttc ccgaccact 
      ggatgaaac acttcttcc tggcacacg catacctcg 
      agcaactga acaacgacg aagctttac aaatttatt 
      tcgaactgt actcacaag ggctcaaaa acagcacct 
      atctaatgg cccagatcg caatcggcc accacaacc 
      ggcgagcca aactcctta acgtaagac actcccccg 
      tcattcacg tttttgcat tggagcaaa acgggcccc 
      acaaccgcc agaaccaag tcagtgatt cgcttgatt 
      tgcttcgtc tcgtgactg gggtccccc aagtaaacc 
      gttttttcc aaaaccatg caaacgcga cggctcaaa 
      accgggata aggggcaca aggcaagtc ctgactgtc 
      tcctccccg aaacaactc aggcctttg aggccccca 
      tacaccgta gtgaggggc ttccggggg tcttgcacc 
      gcccgcaag taaacctta atcacgcgc gttttcatc 
      tggttgatg ctgttactg tgcgtgcaa caaatagtt 
      gccacaact tgcgccgaa aagtccctc aaagtccag 
      tggttgtca tagctgtat ttgctaatt ttccgcgaa 
      acgacacag gaaatgacg gccgggagc agcaaatgg 
      aggtgctcc ttaatgtta acgtggggg ttggccatc 
      aggcttttg accactcgc accgaataa atcaaaaga 
      gacaagttt cgttgcgga cgtgctcgt tgaacccga 
      aagccccct ctgtcaatt taacgaaaa aaacctgct 
      cttcggcgg atagggggt gggggtcag ggtgcagtt 
      tgagtaaaa ttgggtcag gttttggct ggaatttct 
      tggccgggg gttggggta cagcaggcg ggttttttt 
      gttcctggt acagttcca ggtctttta aatattccg 
      acattgttg cagaactat tccacgcga ttgacttgt 
      ttaaggaga ttttaagtg tggatcctt acagactag 
      ataatttgg atacttact cgaatcttt tgtacgtgc 
      aagaaatga agactgagt tggccgatt tggcgcaca 
      aggtggtgc tgattgaca agtacaggg tggagacgt 
      gttgcgtga ttgggaatt actacagtc tgaggtcag 
      atcatgcca aggctgtac tgtactttc gcagggctt 
      tgaagctca atccccagt ttttgtcag cgtggacgc 
      taatggggc acgagtaca tggaaatga aaaacacaa 
      atgcggcga ttcagagct acagacacg caattgaaa 
      ttaataatc gggattacc gggcttggt acggcctcg 
      gccaaacct acgagatcc tcaaaatgt acttctatt 
      gcctctact attacaagc aagcccaac aattaaaac 
      ccaacgaca gtcgcatga ttatcgccc ttggcgaga 
      cctacgaaa agttagaaa agaccgaaa atgccctca 
      agtgttact acaaggcgt gcaaagttg gggacattg 
      aaggccaag ctttgatca aactcgcaa agctttacg 
      ataaattaa aagacgaag ataacgccg ctgccgcgt 
      ttacagagt tttgcttgc gtgacgatg agaacaagg 
      gacggttca atgaagacc aaactgagt tctacagcg 
      ctctccaat atctagcga attattttt taaagaggg 
      gagatttgg accaagcaa agatttacg ccaacaagt 
      gcctggaga atgagaagg cgaaagaag ttgcgaagg 
      ccttgttga aagaaatcg ctgtaaaaa gactactaa 
      ccgaaagct tgggttcag acactgttc caatggatg 
      gaacctgta gtaatattt tgcccccgg acgacgatg 
      gaggctctg atatggaca ttactaata cgtccacca 
      aataatata ttgtgtaca gattaacta tcaataaaa 
      ataccattt ctcataaaa cgactttta tttacaacc 
      tgtacaact ttgcgtaat aaaataaaa ggcataagc 
      agtcaaaaa aattaaaat tgcgtaacc ccctcgaag 
      gaatttgtg ttcttcaac tccgcttgt tcaaatctg 
      gcaataagt ccgttaacg aaagcgtca aagcgttcc 
      aaatttggg ttgtttgga attaaatgt ccactgatg 
      atttctttg aattctcgt ctagtgcca cgaatgcga 
      atttttctc caattgggt gaagagtgc agatttatc 
      acgaatttg tgatacctg ctgtaacat tcacaagac 
      gatgaaact gtgatcctt tcctcctcg gggactgga 
      gacgaagtt ctcgggaac gacatggtg gtgaaaatt 
      ctataacaa ttaaaaaag atatgagtt a
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