Status
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Gene information
General information
TC014478 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10906200..10908018
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014478
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02290

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva proximal constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax segment random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014478 RA
      MSASYNLKI CSFFVILAV ARGGLNPVV LIPGDGGSQ 
      VEAKLNKSA SVHYICEKT TSDFFNIWL NMELLVPLV 
      IDCWIDNIK LIYDNATRT TRNNDGVEI RIPGFGGTE 
      TVEWLDPSH ASAGAYFNS IAKTLVSLG HERNKTMKG 
      APYDFRKAP NENQQFFTD LKALIEQTY TENNNQPVI 
      IIAHSMGGP MSLFFLNQQ TQDWKDKYI RSLVTLSGA 
      WGGSMKAVK VYAIGDDLG SYVLRESVM REEQITSPS 
      LAWLLPSKL FWKPDEVLV QTSRKNFSL NNLEEFFQA 
      INFPNGWEM RKDTEKYQL DYRPPGVEV HCLYGVGVD 
      TVERLFYKP GTWLDGYPT LINGDGDGT VNRRSLEGC 
      LHWESLQKQ KVYSKQLPK VDHMQILNN KDVLSYIAN 
      LINDV
    • >TC014478 RB
      MELLVPLVI DCWIDNIKL IYDNATRTT RNNDGVEIR 
      IPGFGGTET VEWLDPSHA SAGAYFNSI AKTLVSLGH 
      ERNKTMKGA PYDFRKAPN ENQQFFTDL KALIEQTYT 
      ENNNQPVII IAHSMGGPM SLFFLNQQT QDWKDKYIR 
      SLVTLSGAW GGSMKAVKV YAIGDDLGS YVLRESVMR 
      EEQITSPSL AWLLPSKLF WKPDEVLVQ TSRKNFSLN 
      NLEEFFQAI NFPNGWEMR KDTEKYQLD YRPPGVEVH 
      CLYGVGVDT VERLFYKPG TWLDGYPTL INGDGDGTV 
      NRRSLEGCL HWESLQKQK VYSKQLPKV DHMQILNNK 
      DVLSYIANL INDV
    CDS sequence
    • >TC014478 RA
      atgtcagcc tcatataac ctcaaaatc tgtagtttt 
      ttcgtaatt ttggcggta gccagaggc ggtttaaac 
      cccgttgtg ttaatccca ggcgatggg ggctctcaa 
      gtcgaagcc aaactcaac aaatcagcc tcagtgcat 
      tacatttgt gaaaagaca acgtcggat tttttcaac 
      atatggctc aacatggag ctgctggtg cccctagtc 
      atcgactgc tggattgac aatattaaa ctgatctat 
      gataacgcc actcgaaca acgcgcaat aacgacggg 
      gtcgagatc aggatcccc ggctttggg ggcaccgag 
      actgtggag tggctggac cccagccat gcctctgca 
      ggtgcgtat ttcaacagc attgccaag accttggtc 
      agtctcggc cacgagcgc aacaaaacc atgaaaggg 
      gcaccttac gacttccgc aaagcgccc aacgaaaac 
      caacagttt tttaccgat ctcaaggcc ctaattgaa 
      caaacgtac acggaaaat aacaatcag ccggtcatt 
      atcattgca cattcgatg gggggcccc atgtctttg 
      tttttcctg aaccagcaa acgcaagac tggaaagac 
      aagtacatc aggtctttg gttacgcta agtggggcc 
      tgggggggc tccatgaaa gcggtcaaa gtctacgcg 
      attggggac gatttgggg tcgtacgtt ttgcgggag 
      agcgtcatg cgggaggag cagatcacc tcccccagc 
      ctggcgtgg ctcctcccc tccaagctc ttctggaag 
      ccggacgaa gtcttggtg caaaccagt cggaaaaat 
      ttcagtttg aataacctg gaggagttt ttccaagcg 
      attaatttc cccaacggg tgggaaatg cgcaaagac 
      actgagaag tatcagctg gattaccgg ccgccaggg 
      gttgaggtg cactgcttg tacggggtc ggggttgat 
      acggttgaa cggttgttt tacaagcca gggacgtgg 
      ttggatggg taccccact ttgatcaat ggggacggg 
      gacgggact gtgaaccgg aggtcgctt gaagggtgt 
      ctccactgg gagagcctc cagaaacaa aaagtctac 
      tcgaagcag cttcccaaa gtcgatcat atgcaaatt 
      ctcaacaat aaggacgtt ctgagctac attgccaat 
      ctcattaac gacgtgtga
    • >TC014478 RB
      atggagctg ctggtgccc ctagtcatc gactgctgg 
      attgacaat attaaactg atctatgat aacgccact 
      cgaacaacg cgcaataac gacggggtc gagatcagg 
      atccccggc tttgggggc accgagact gtggagtgg 
      ctggacccc agccatgcc tctgcaggt gcgtatttc 
      aacagcatt gccaagacc ttggtcagt ctcggccac 
      gagcgcaac aaaaccatg aaaggggca ccttacgac 
      ttccgcaaa gcgcccaac gaaaaccaa cagtttttt 
      accgatctc aaggcccta attgaacaa acgtacacg 
      gaaaataac aatcagccg gtcattatc attgcacat 
      tcgatgggg ggccccatg tctttgttt ttcctgaac 
      cagcaaacg caagactgg aaagacaag tacatcagg 
      tctttggtt acgctaagt ggggcctgg gggggctcc 
      atgaaagcg gtcaaagtc tacgcgatt ggggacgat 
      ttggggtcg tacgttttg cgggagagc gtcatgcgg 
      gaggagcag atcacctcc cccagcctg gcgtggctc 
      ctcccctcc aagctcttc tggaagccg gacgaagtc 
      ttggtgcaa accagtcgg aaaaatttc agtttgaat 
      aacctggag gagtttttc caagcgatt aatttcccc 
      aacgggtgg gaaatgcgc aaagacact gagaagtat 
      cagctggat taccggccg ccaggggtt gaggtgcac 
      tgcttgtac ggggtcggg gttgatacg gttgaacgg 
      ttgttttac aagccaggg acgtggttg gatgggtac 
      cccactttg atcaatggg gacggggac gggactgtg 
      aaccggagg tcgcttgaa gggtgtctc cactgggag 
      agcctccag aaacaaaaa gtctactcg aagcagctt 
      cccaaagtc gatcatatg caaattctc aacaataag 
      gacgttctg agctacatt gccaatctc attaacgac 
      gtgtga
    mRNA sequence
    • >TC014478 RA
      tggtttgtg acccgttca aagttcaaa aaaagattc 
      acttgtgat tgggatggt gtgatgtgt aacccgcgt 
      cagcatacc tccaaaaaa tcgtttcat ttctgttta 
      ttttgcata aaacggctc tttttgtct ttgttcagt 
      aaataaaca atccgccgc cactggtaa caaacgtca 
      aaatcatct gtggtcatt aattgtcaa ttgtatcca 
      aaatatcag tgtttttca ataagaaaa tatcatggt 
      cgatttaac tcgtcccag cgaaccgac ctgagtgag 
      tgaatttaa ttattatct tcccagtca cgccaaaat 
      taaccgtga aagcaaaaa ttcgacgat gtcagcctc 
      atataacct caaaatctg tagtttttt cgtaatttt 
      ggcggtagc cagaggcgg tttaaaccc cgttgtgtt 
      aatcccagg cgatggggg ctctcaagt cgaagccaa 
      actcaacaa atcagcctc agtgcatta catttgtga 
      aaagacaac gtcggattt tttcaacat atggctcaa 
      catggagct gctggtgcc cctagtcat cgactgctg 
      gattgacaa tattaaact gatctatga taacgccac 
      tcgaacaac gcgcaataa cgacggggt cgagatcag 
      gatccccgg ctttggggg caccgagac tgtggagtg 
      gctggaccc cagccatgc ctctgcagg tgcgtattt 
      caacagcat tgccaagac cttggtcag tctcggcca 
      cgagcgcaa caaaaccat gaaaggggc accttacga 
      cttccgcaa agcgcccaa cgaaaacca acagttttt 
      taccgatct caaggccct aattgaaca aacgtacac 
      ggaaaataa caatcagcc ggtcattat cattgcaca 
      ttcgatggg gggccccat gtctttgtt tttcctgaa 
      ccagcaaac gcaagactg gaaagacaa gtacatcag 
      gtctttggt tacgctaag tggggcctg ggggggctc 
      catgaaagc ggtcaaagt ctacgcgat tggggacga 
      tttggggtc gtacgtttt gcgggagag cgtcatgcg 
      ggaggagca gatcacctc ccccagcct ggcgtggct 
      cctcccctc caagctctt ctggaagcc ggacgaagt 
      cttggtgca aaccagtcg gaaaaattt cagtttgaa 
      taacctgga ggagttttt ccaagcgat taatttccc 
      caacgggtg ggaaatgcg caaagacac tgagaagta 
      tcagctgga ttaccggcc gccaggggt tgaggtgca 
      ctgcttgta cggggtcgg ggttgatac ggttgaacg 
      gttgtttta caagccagg gacgtggtt ggatgggta 
      ccccacttt gatcaatgg ggacgggga cgggactgt 
      gaaccggag gtcgcttga agggtgtct ccactggga 
      gagcctcca gaaacaaaa agtctactc gaagcagct 
      tcccaaagt cgatcatat gcaaattct caacaataa 
      ggacgttct gagctacat tgccaatct cattaacga 
      cgtgtgatt tagaaatta ccgaaattt taacgctgt 
      tacttccct tctcttcct gattttgag tgttttatt 
      tatgttttg tattttgtg atatgtata ttggaaata 
      acaataaaa aagattgag agtt
    • >TC014478 RB
      tggtttgtg acccgttca aagttcaaa aaaagattc 
      acttgtgat tgggatggt gtgatgtgt aacccgcgt 
      cagcatacc tccaaaaaa tcgtttcat ttctgttta 
      ttttgcata aaacggctc tttttgtct ttgttcagt 
      aaataaaca atccgccgc cactggtaa caaacgtca 
      aaatcatct gtggtcatt aattgtcaa ttgtatcca 
      aaatatcag tgtttttca ataagaaaa tatcatggt 
      cgatttaac tcgtcccag cgaaccgac ctgagcgat 
      gggggctct caagtcgaa gccaaactc aacaaatca 
      gcctcagtg cattacatt tgtgaaaag acaacgtcg 
      gattttttc aacatatgg ctcaacatg gagctgctg 
      gtgccccta gtcatcgac tgctggatt gacaatatt 
      aaactgatc tatgataac gccactcga acaacgcgc 
      aataacgac ggggtcgag atcaggatc cccggcttt 
      gggggcacc gagactgtg gagtggctg gaccccagc 
      catgcctct gcaggtgcg tatttcaac agcattgcc 
      aagaccttg gtcagtctc ggccacgag cgcaacaaa 
      accatgaaa ggggcacct tacgacttc cgcaaagcg 
      cccaacgaa aaccaacag ttttttacc gatctcaag 
      gccctaatt gaacaaacg tacacggaa aataacaat 
      cagccggtc attatcatt gcacattcg atggggggc 
      cccatgtct ttgtttttc ctgaaccag caaacgcaa 
      gactggaaa gacaagtac atcaggtct ttggttacg 
      ctaagtggg gcctggggg ggctccatg aaagcggtc 
      aaagtctac gcgattggg gacgatttg gggtcgtac 
      gttttgcgg gagagcgtc atgcgggag gagcagatc 
      acctccccc agcctggcg tggctcctc ccctccaag 
      ctcttctgg aagccggac gaagtcttg gtgcaaacc 
      agtcggaaa aatttcagt ttgaataac ctggaggag 
      tttttccaa gcgattaat ttccccaac gggtgggaa 
      atgcgcaaa gacactgag aagtatcag ctggattac 
      cggccgcca ggggttgag gtgcactgc ttgtacggg 
      gtcggggtt gatacggtt gaacggttg ttttacaag 
      ccagggacg tggttggat gggtacccc actttgatc 
      aatggggac ggggacggg actgtgaac cggaggtcg 
      cttgaaggg tgtctccac tgggagagc ctccagaaa 
      caaaaagtc tactcgaag cagcttccc aaagtcgat 
      catatgcaa attctcaac aataaggac gttctgagc 
      tacattgcc aatctcatt aacgacgtg tgatttaga 
      aattaccga aattttaac gctgttact tcccttctc 
      ttcctgatt ttgagtgtt ttatttatg ttttgtatt 
      ttgtgatat gtatattgg aaataacaa taaaaaaga 
      ttgagagtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY