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Gene information
General information
TC014471 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 2804435..2806884
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014471
    iBeetle screen iB_02289
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Fertility
    egg chamber number increased - Number of animals showing the phenotype: 3

    egg chamber size decreased - Number of animals showing the phenotype: 3

    egg chamber shape irregular - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber degenerated - Number of animals showing the phenotype: 4

    Comment:
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014471 RA
      MSGEQEGIR YRRAQSVTR AEEIQAVKQ KVRKSQPDK 
      PIHKPRDSL LSWNSGFEN FTGFVNWAF LLLSIGGLR 
      LLLENFIKY GIRVDPVQW FYILTGQDE QGTEHPSIV 
      LILYSVVPI IFCLLTEKA LAVEIISRR MGMMMHVVN 
      LLVLIFLPM VVIHYKDGF SLVGASTVT TLYSVLFLK 
      LWSYVQVNL WCRNARQSS NNKSLRRQS LSYNNLPKD 
      EDSNKNHKD SKDDLSNTE RLLVQYPDN LNLRDLFYF 
      LCAPTLCYE LNFPRTERI RKRFLLKRI FEVLAGIQL 
      ILCIFQQYM IPSVKNSLI PFSNMDVAL ASERLLKLA 
      IPNHLAWLC MFYILFNSW LNLLGEILH FADRNFYGD 
      WWNANNIDT FWRTWNLPV HRWALRHLY FPLVELGYG 
      KQVAGITVF FISAFFHEY MVSVPLKTY KIWAFMGMM 
      GQIPLSNIS KFMERSYGA RMGNIVVWA SLIIGQPLC 
      IMMYYHDYV ITHYGQSLL EDYGHV
    CDS sequence
    • >TC014471 RA
      atgtcaggt gaacaagaa ggtatcaga tacagaaga 
      gcacaaagt gttacaaga gccgaagag atacaagcc 
      gtcaaacaa aaagtcaga aaatcgcag cccgataag 
      ccgatacac aaaccgcgg gattccctg ctatcatgg 
      aattcgggc ttcgagaat ttcacaggg ttcgtcaat 
      tgggccttt ttgctgtta tcaatcggg gggctgcgc 
      ctcctactc gagaatttc atcaagtat ggcattcga 
      gtggacccg gtccagtgg ttttacata ctcaccggc 
      caggatgaa caaggaacg gagcatccg tccatagtt 
      ttgatactc tattcggtg gttcccata attttttgc 
      cttctcacc gaaaaagct ctagctgtt gaaattatt 
      tcgcgtcgt atggggatg atgatgcat gtggtcaat 
      cttctagtc ctgatcttt ctaccaatg gttgtcata 
      cattacaaa gacgggttt agtttagtg ggggcttcc 
      accgttacc accctgtat agtgtctta tttttgaaa 
      ttgtggtcg tacgtccag gtgaattta tggtgccgt 
      aacgcgcgc caaagttca aacaataag agtctccgg 
      cgccagtct ctctcgtac aacaacttg ccgaaagac 
      gaagacagt aacaaaaac cacaaagac tccaaagac 
      gatttgagc aataccgag agactcctc gtgcaatac 
      cctgacaat ctaaactta cgagacttg ttttacttc 
      ctgtgcgct cccacgctg tgctacgaa ttgaacttt 
      ccgcgcacg gagcgcatc cggaaacgc ttcctcctg 
      aaacgaatc ttcgaagtt ctggcgggc atccaacta 
      atcttgtgc atcttccag caatacatg atcccgtcc 
      gtgaagaat tcgctcatt ccgttcagc aacatggac 
      gtagcgctc gcgtccgaa cgcttgctg aaactggcg 
      attcccaac cacttggca tggctgtgc atgttttac 
      attctgttc aactcgtgg cttaattta ttaggggaa 
      atactgcac tttgccgat cggaatttc tacggggat 
      tggtggaat gccaacaac attgacacg ttctggcgc 
      acgtggaat ttgccggtg catcgctgg gcgttgaga 
      cacttgtat tttccttta gtggagctg ggttacggc 
      aagcaggtc gccgggatc accgtgttt ttcatcagt 
      gccttcttc cacgagtac atggtcagc gtgccgttg 
      aagacgtac aagatctgg gccttcatg ggcatgatg 
      ggccagatc ccgctcagc aacatctcc aagttcatg 
      gagcgcagc tacggggcc cgaatgggc aatatcgtg 
      gtctgggcc tcgctgata atcgggcag ccgctctgc 
      atcatgatg tactaccac gactacgtc atcacccat 
      tacgggcag tcgctgctg gaggactac gggcatgtg 
      taa
    mRNA sequence
    • >TC014471 RA
      gataagata aagtcatgg gggtcaaag gtcgcgggt 
      tgggttagg tccgtatcc gttttttgg ggacctcct 
      gtagtaatt agtttgccc caaaactac tttccgcgt 
      gactttcat ttaaaattg ccttccaga tccacctct 
      ttcggagat tgcttcaca catggtcat ggtcgctct 
      ctttccaat tcgtaccat ttcggtata tttatctct 
      gtttaatat aattgtcgc gacgtcagt gcggcctga 
      taacccccc ttaaaccat gttagatct atgtccgtg 
      tttatttct tccatttat cagtttgag acactcgaa 
      aaagcgata aatgtgcaa gtgtcggct cgaagatgt 
      caggtgaac aagaaggta tcagataca gaagagcac 
      aaagtgtta caagagccg aagagatac aagccgtca 
      aacaaaaag tcagaaaat cgcagcccg ataagccga 
      tacacaaac cgcgggatt ccctgctat catggaatt 
      cgggcttcg agaatttca cagggttcg tcaattggg 
      cctttttgc tgttatcaa tcggggggc tgcgcctcc 
      tactcgaga atttcatca agtatggca ttcgagtgg 
      acccggtcc agtggtttt acatactca ccggccagg 
      atgaacaag gaacggagc atccgtcca tagttttga 
      tactctatt cggtggttc ccataattt tttgccttc 
      tcaccgaaa aagctctag ctgttgaaa ttatttcgc 
      gtcgtatgg ggatgatga tgcatgtgg tcaatcttc 
      tagtcctga tctttctac caatggttg tcatacatt 
      acaaagacg ggtttagtt tagtggggg cttccaccg 
      ttaccaccc tgtatagtg tcttatttt tgaaattgt 
      ggtcgtacg tccaggtga atttatggt gccgtaacg 
      cgcgccaaa gttcaaaca ataagagtc tccggcgcc 
      agtctctct cgtacaaca acttgccga aagacgaag 
      acagtaaca aaaaccaca aagactcca aagacgatt 
      tgagcaata ccgagagac tcctcgtgc aataccctg 
      acaatctaa acttacgag acttgtttt acttcctgt 
      gcgctccca cgctgtgct acgaattga actttccgc 
      gcacggagc gcatccgga aacgcttcc tcctgaaac 
      gaatcttcg aagttctgg cgggcatcc aactaatct 
      tgtgcatct tccagcaat acatgatcc cgtccgtga 
      agaattcgc tcattccgt tcagcaaca tggacgtag 
      cgctcgcgt ccgaacgct tgctgaaac tggcgattc 
      ccaaccact tggcatggc tgtgcatgt tttacattc 
      tgttcaact cgtggctta atttattag gggaaatac 
      tgcactttg ccgatcgga atttctacg gggattggt 
      ggaatgcca acaacattg acacgttct ggcgcacgt 
      ggaatttgc cggtgcatc gctgggcgt tgagacact 
      tgtattttc ctttagtgg agctgggtt acggcaagc 
      aggtcgccg ggatcaccg tgtttttca tcagtgcct 
      tcttccacg agtacatgg tcagcgtgc cgttgaaga 
      cgtacaaga tctgggcct tcatgggca tgatgggcc 
      agatcccgc tcagcaaca tctccaagt tcatggagc 
      gcagctacg gggcccgaa tgggcaata tcgtggtct 
      gggcctcgc tgataatcg ggcagccgc tctgcatca 
      tgatgtact accacgact acgtcatca cccattacg 
      ggcagtcgc tgctggagg actacgggc atgtgtaac 
      gactgatta ggtttaggt tttttattg gttaccatt 
      gtttaataa aactgtgag aaaacg