Status
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Gene information
General information
TC014465 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10680279..10684566
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014465
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02288

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber complete not present - Number of animals showing the phenotype: 5
    vitellogenic egg chamber late completenot present - Number of animals showing the phenotype: 5

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    Pupal morphology
    eyes decreased - Number of animals showing the phenotype: 4

    ommatidia irregular - Number of animals showing the phenotype: 4
    Adult morphology
    eyes decreased - Number of animals showing the phenotype: 4


    ommatidia irregular - Number of animals showing the phenotype: 4

    elytra distalpigmentation/tanning decreased - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014465 RA
      MADTGYKNR IQNFKNKGK DQDEMRRRR NEVTVELRK 
      NKREETLQK RRNVPVVDS TDEDDIEKS LSTTNLEEL 
      VAQAAAIDN PPLQLMAIQ TARKLLSSD RNPPIDALI 
      SSGILPVLV MCLERYDEP SLQFEAAWA LTNIASGTS 
      AQTNRVVQA GAVPLFLKL LHSPQQNVC EQAVWALGN 
      IIGDGPQLR DYVIELGVV NPLLSFIKP DVPISFLRN 
      VTWVIVNLC RNKDPPPPI QTIRELLPA LNALIHHTD 
      TNILVDTVW ALSYLTDGG NEQIQMVID SGVVPKLIP 
      LLSHKEVKV QTAALRAVG NIVTGTDEQ TQVVLNCDA 
      LSHFPALLS HSKEKICKE AVWFLSNIT AGNQMQVQA 
      VIDAGLLPN IINNLSKGD FQTQKEAAW AISNLTIGG 
      NKEQVATLI REGVIPPFC DLLSCKDAQ VIQVVLDGI 
      NNMLKMAGA EVDQLCTMI EECNGLDKI EMLQNHDNI 
      EIYKIAYDI IEQYFSGDA DDDANLAPT AAETGFQFD 
      PNTNVPNEG FKF
    CDS sequence
    • >TC014465 RA
      atggcggat acaggttac aagaatcgc atccagaat 
      ttcaagaac aagggaaaa gaccaagat gaaatgcga 
      agacggcgc aacgaggtc accgtggaa ttgcgcaag 
      aacaagcgc gaggagacc ctccagaag cgccgcaat 
      gtccccgtg gtggactcc accgacgag gacgacatc 
      gagaagtcg ctctccacc acgaacttg gaagaactg 
      gtggcgcaa gcggccgct atcgacaac cccccgctg 
      cagctgatg gcgatccaa accgcgcgg aagttgctg 
      tcttcagac aggaacccc ccgatcgac gccctcatc 
      tccagcggg atcctgccg gtcctggtg atgtgtctc 
      gaacggtat gacgagccc tcgttgcag tttgaggcc 
      gcctgggcg ctcacgaac atcgcctcg ggcacgtcc 
      gcgcagacc aacagggtg gtgcaagcg ggggccgtg 
      cccctcttc ctgaagctg ctgcactcg ccccagcag 
      aacgtctgt gaacaggcc gtttgggcc ctggggaat 
      atcatcggc gatgggccg cagttaaga gactatgtt 
      atcgagctc ggtgttgtt aatccactt ctctcgttc 
      attaaaccc gacgttccg atttcgttc ttgaggaac 
      gtcacctgg gttattgtt aatttgtgt aggaacaag 
      gatcccccg ccgcctata cagactatt agggagttg 
      ttacccgcc ttgaatgcg cttattcac cacacagat 
      accaatatt ttagtcgac acggtctgg gcattaagt 
      tacctcacc gacggcggc aacgaacaa attcaaatg 
      gtgatcgac agcggcgtc gtgcccaaa ctcatccct 
      ctcctcagc cacaaggag gtgaaagtg cagacggcg 
      gccctccgt gccgtcgga aacatcgtc accggaacg 
      gacgagcaa acccaggtg gtcctcaac tgcgacgcc 
      ttgtcccac ttccctgcc ctcctctcc cactccaaa 
      gagaagatc tgtaaagag gccgtctgg ttcctgtct 
      aacatcacc gccggtaac cagatgcaa gtccaggcc 
      gtgattgac gccggcctc ctgcccaac atcatcaac 
      aacctcagc aagggcgat tttcaaacg cagaaagag 
      gcggcgtgg gccatcagt aatctaacc attggcgga 
      aacaaggaa caggtggcc acactgatc cgcgaaggg 
      gtgattccc ccgttctgc gacctcctc agttgtaaa 
      gatgcgcaa gtgattcag gtcgttttg gacgggatt 
      aacaacatg ctgaagatg gcgggagcg gaggtggac 
      cagttgtgt accatgatt gaggagtgc aatggctta 
      gataaaatc gaaatgttg caaaatcat gacaatata 
      gaaatttat aaaattgca tatgacatc atcgaacag 
      tactttagt ggtgatgcg gatgatgat gcaaactta 
      gcaccgacc gccgctgaa accggattc caattcgat 
      ccaaatacc aacgtaccg aacgaggga ttcaaattc 
      tga
    mRNA sequence
    • >TC014465 RA
      gccaacttt aaaaactga aagttttgc cgaaattat 
      tgcaaataa aggtgtagt gtccattga aacaataaa 
      tttggcttg attttgagc ctacagtga aaagttgtg 
      gttttaatg ttaataagg tttagattg tgttaattt 
      tcccgatca gctgtcctt ccgttgtgt ttgccaccg 
      tgcgcgcaa cctgtgcaa ccgatcttt gtagcaaat 
      gggtatgaa aaaaatacc caaattacg ttaaatcag 
      tcggtaatt ttgtgataa tcgcgtccg aacgtttcc 
      cccttcatt tttcatgtt atcataacg ccaaatcga 
      gtcttttac gttttaaga gcgccaccc ggcttttcc 
      tcgtgtttt catcagccc tccattgtc aaatctatc 
      aaaatggcg gatacaggt tacaagaat cgcatccag 
      aatttcaag aacaaggga aaagaccaa gatgaaatg 
      cgaagacgg cgcaacgag gtcaccgtg gaattgcgc 
      aagaacaag cgcgaggag accctccag aagcgccgc 
      aatgtcccc gtggtggac tccaccgac gaggacgac 
      atcgagaag tcgctctcc accacgaac ttggaagaa 
      ctggtggcg caagcggcc gctatcgac aaccccccg 
      ctgcagctg atggcgatc caaaccgcg cggaagttg 
      ctgtcttca gacaggaac cccccgatc gacgccctc 
      atctccagc gggatcctg ccggtcctg gtgatgtgt 
      ctcgaacgg tatgacgag ccctcgttg cagtttgag 
      gccgcctgg gcgctcacg aacatcgcc tcgggcacg 
      tccgcgcag accaacagg gtggtgcaa gcgggggcc 
      gtgcccctc ttcctgaag ctgctgcac tcgccccag 
      cagaacgtc tgtgaacag gccgtttgg gccctgggg 
      aatatcatc ggcgatggg ccgcagtta agagactat 
      gttatcgag ctcggtgtt gttaatcca cttctctcg 
      ttcattaaa cccgacgtt ccgatttcg ttcttgagg 
      aacgtcacc tgggttatt gttaatttg tgtaggaac 
      aaggatccc ccgccgcct atacagact attagggag 
      ttgttaccc gccttgaat gcgcttatt caccacaca 
      gataccaat attttagtc gacacggtc tgggcatta 
      agttacctc accgacggc ggcaacgaa caaattcaa 
      atggtgatc gacagcggc gtcgtgccc aaactcatc 
      cctctcctc agccacaag gaggtgaaa gtgcagacg 
      gcggccctc cgtgccgtc ggaaacatc gtcaccgga 
      acggacgag caaacccag gtggtcctc aactgcgac 
      gccttgtcc cacttccct gccctcctc tcccactcc 
      aaagagaag atctgtaaa gaggccgtc tggttcctg 
      tctaacatc accgccggt aaccagatg caagtccag 
      gccgtgatt gacgccggc ctcctgccc aacatcatc 
      aacaacctc agcaagggc gattttcaa acgcagaaa 
      gaggcggcg tgggccatc agtaatcta accattggc 
      ggaaacaag gaacaggtg gccacactg atccgcgaa 
      ggggtgatt cccccgttc tgcgacctc ctcagttgt 
      aaagatgcg caagtgatt caggtcgtt ttggacggg 
      attaacaac atgctgaag atggcggga gcggaggtg 
      gaccagttg tgtaccatg attgaggag tgcaatggc 
      ttagataaa atcgaaatg ttgcaaaat catgacaat 
      atagaaatt tataaaatt gcatatgac atcatcgaa 
      cagtacttt agtggtgat gcggatgat gatgcaaac 
      ttagcaccg accgccgct gaaaccgga ttccaattc 
      gatccaaat accaacgta ccgaacgag ggattcaaa 
      ttctgattc atttctata ttgctcaga gtaactatt 
      ttgatctag cgcgtttgc ttcctttcc tccctaaat 
      tcttttacg gtggtttat gtacgctca ttttatacg 
      gttgtgtaa atatagaat tttttataa ataattatg 
      ctcgtaatt gctttagga tatttattt aatttaaac 
      ggaaaaata tgtcactgt attttagtt tttatttta 
      ttttgtaca tttaaatta ggtaaatat caacattaa 
      tgtaaaatt tttaaaatg accgtacat aagagacaa 
      tctgggcat gatctaata aatcaaaaa acagtaagt 
      cattaacgg gattttaac aattggaca tgtcatgcg 
      cagatttgt atattttta tgatattaa cagtttttt 
      ggtaaacat catgctata tacctgcgt gttttttaa 
      gtttacaac gtttttttt tcgttgttt gtagtcata 
      tacataatg tttatcgaa aaagaacta aataaattg 
      agaatttta attgactgg tcgcgttta attaatatg 
      attaaggct attatgtaa ttaaaaagt atagtttcg 
      tgcttacca ttgtattat ggtagttgg catcgttta 
      aatataatt taaaataaa caaagtcga atctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY