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Gene information
General information
TC014441 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10466257..10467941
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014441
    iBeetle screen iB_02286

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva anterior partiallydecreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014441 RA
      MYYLAQFCR ICIKTGVKL LDINTVDFD YVTFSEKLE 
      SCSKMVINR ESLSTQICL QCVKKLRMS YDFHNMCKQ 
      STKILQGYL TELISASNK ITPESFVNS ELRVTLTPI 
      CKNALKRKR ITKYQRCSL LKKLLLSRK NNIRKEKTP 
      RKKREPKSV YKGGLRGII DFTRNFDFG YKYDGNHNN 
      CLLSDATPL SRLHNFTNT FFLNNFAEF RNTILYIIE 
      NKDNLSESD DDDDMFDSY IPEPGEPEV KSEEVVVEP 
      DIKIKTEYD YDDSETNFS CNYYVETSY SDSEIKKEN 
      FEVEDHPIK QEPLDYNNE PQNSVQMLD KLVGDLGQQ 
      RRAFSHHSV RCRTRGNPY INPKLKQQF QFKSFQCDK 
      CSRYFKSPG YLKAHIAKI HH
    CDS sequence
    • >TC014441 RA
      atgtattat ttggcgcag ttttgtcgg atttgtata 
      aaaacgggc gtgaaattg ctggacata aacactgtt 
      gactttgac tatgtgaca ttcagtgag aaactcgag 
      tcgtgctcg aaaatggtt atcaacaga gaaagtttg 
      tcaacacag atctgcctc caatgcgtg aagaagcta 
      cgaatgtcg tacgatttc cacaacatg tgcaaacag 
      tcaacgaag atcctccaa ggatacctg accgaactc 
      ataagcgcg tcaaataaa atcactccc gaatcgttt 
      gtcaattcc gaactgcgg gtcaccctc acccccatt 
      tgtaaaaat gcgctcaag cggaaacgc atcaccaaa 
      taccagcgg tgctccctc ctcaaaaaa ctcctcctc 
      tcccggaaa aacaacatt cgcaaggag aaaaccccg 
      cggaaaaaa cgcgaaccc aagtccgtc tataaaggc 
      ggcctccgg ggcataatc gacttcacg cgaaacttc 
      gacttcggg tacaaatac gacggcaac cacaacaac 
      tgtctcctg agcgacgcc acccccttg agccgcctc 
      cacaacttc accaacacg tttttcctc aacaatttc 
      gccgagttc aggaacacc atcctctac atcatcgag 
      aataaagac aacctttcg gaaagcgac gatgacgat 
      gacatgttt gactcgtac atccctgag ccgggcgag 
      cccgaggtc aagtcggag gaagtcgtt gttgagccc 
      gacatcaaa attaagaca gagtacgac tatgacgac 
      tctgagacg aatttctcg tgtaattat tacgtggag 
      acttcatac agcgatagc gagatcaag aaagagaat 
      ttcgaagtt gaggatcac cccatcaaa caggaacct 
      ctagactat aataatgaa ccccagaac tccgtgcaa 
      atgctcgat aagttagtg ggggacttg gggcagcaa 
      agacgcgcc ttttcgcac cacagcgtg cggtgtcgg 
      acaaggggc aacccctac atcaacccc aaattgaag 
      caacaattt cagtttaaa agtttccag tgcgacaaa 
      tgcagccga tatttcaaa agtccgggc tatctcaag 
      gcgcatatc gcgaaaata caccattga
    mRNA sequence
    • >TC014441 RA
      catttatac aaaaattat aaataatgt attatttgg 
      cgcagtttt gtcggattt gtataaaaa cgggcgtga 
      aattgctgg acataaaca ctgttgact ttgactatg 
      tgacattca gtgagaaac tcgagtcgt gctcgaaaa 
      tggttatca acagagaaa gtttgtcaa cacagatct 
      gcctccaat gcgtgaaga agctacgaa tgtcgtacg 
      atttccaca acatgtgca aacagtcaa cgaagatcc 
      tccaaggat acctgaccg aactcataa gcgcgtcaa 
      ataaaatca ctcccgaat cgtttgtca attccgaac 
      tgcgggtca ccctcaccc ccatttgta aaaatgcgc 
      tcaagcgga aacgcatca ccaaatacc agcggtgct 
      ccctcctca aaaaactcc tcctctccc ggaaaaaca 
      acattcgca aggagaaaa ccccgcgga aaaaacgcg 
      aacccaagt ccgtctata aaggcggcc tccggggca 
      taatcgact tcacgcgaa acttcgact tcgggtaca 
      aatacgacg gcaaccaca acaactgtc tcctgagcg 
      acgccaccc ccttgagcc gcctccaca acttcacca 
      acacgtttt tcctcaaca atttcgccg agttcagga 
      acaccatcc tctacatca tcgagaata aagacaacc 
      tttcggaaa gcgacgatg acgatgaca tgtttgact 
      cgtacatcc ctgagccgg gcgagcccg aggtcaagt 
      cggaggaag tcgttgttg agcccgaca tcaaaatta 
      agacagagt acgactatg acgactctg agacgaatt 
      tctcgtgta attattacg tggagactt catacagcg 
      atagcgaga tcaagaaag agaatttcg aagttgagg 
      atcacccca tcaaacagg aacctctag actataata 
      atgaacccc agaactccg tgcaaatgc tcgataagt 
      tagtggggg acttggggc agcaaagac gcgcctttt 
      cgcaccaca gcgtgcggt gtcggacaa ggggcaacc 
      cctacatca accccaaat tgaagcaac aatttcagt 
      ttaaaagtt tccagtgcg acaaatgca gccgatatt 
      tcaaaagtc cgggctatc tcaaggcgc atatcgcga 
      aaatacacc attgaggag ggcgccctc tttggcggg 
      tttatagtt tccagaaat tttgttacg gaagaatga 
      tagtgattg ttaagtgaa accgtttca ctaaaaatg 
      ttcggtaag gctaaaagc agttttgtg atttaaatg 
      ttgtaaata attgtattt atgcttgcg cgcatccgc 
      gtttccaga ttttataga tttaagcct tctcgacgt 
      gatgaatcg actcatcac cgttttaat ccgaattta 
      tagatgtga gtgtaacag ctaggttag tgcaacgta 
      atcaaaagt cagtattgc aatatttgc caagcatcg 
      aaattttct tcaagttgg tgtcagttg gatattttt 
      gatattatt ggaccgttt ttacgccat ttcttgttt 
      taactgaat tgttgattg ttttatata atttttata 
      tattttttt attttatca ttgtctcaa ataaattta 
      ttcgattga g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY