Status
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Gene information
General information
TC014403 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10151492..10156749
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014403
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02284

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014403 RA
      MDSSKKYDR PSPEETANP LSKLFFAWT LPFFKYGYS 
      NDLQLKDLY NCAKYDLSG KLGDDIERN WLLEVKSSL 
      KNNKKPSLL RTIRQTFIT PYSLTGVIL CLQCAVLKM 
      FQPIVLAWY INYYGVNYN DRSSGTGWG LATGVILLA 
      FVNIMIIHH CNLYSQRIG MRSRIATCS LIYRKLLRL 
      NRTSLGQVA VGKLVNLLS NDVQRFDIT STFLHYIWI 
      MPIQAIIAF YVMYRSVGL AALSGLGAI TLQAVFLQG 
      YLSRLQGIY RMQIAQKTD NRVKLMNEI TAGIQVIKM 
      YAWEKPFEK VVALARKYE INIVAKTSY IRGFSIALM 
      VFTERTASY LTLITFVLM GNVLSADVV FSVAQLFNT 
      VQLYMCILY PLGISTFAE AKTSVKRIE EFLLLEENE 
      TGADLSVTQ SEKNGSIRL DKVNASWTP NPIVHTLMD 
      LNIEINPGT LCCVVGPVG AGKSSLLQL LLKELPANS 
      GRVSLTGNI SYASQEPWL FVSSVRNNI LFGQQYLKN 
      RYKDVVKVC ALETDFEQF PHGDKTLVG ERGTSLSGG 
      QRARVNLAR AVYRDADIY LFDDPLSAV DAHVGKHLF 
      NECIVKYLK DKTRVLVTH QLQFLRQAD LIIVLNNGQ 
      VEKIGTFAE LSENELNNL KLEQPEEVA TKEQEMQQR 
      QRLMSIASH NSTVPDDDD EIEAQETEE LMEKGAIPT 
      STYLDYYRS GGSIAVLLF LVVLLIIAQ ASCNASDLW 
      LTYWTNLEQ KSPAIVNGD SNTTEMPFS GTNDTNTEP 
      PTELNTTEI ISTALPPVF NLTDPNEPL SRATYIWVY 
      SILILFSII LTTSRSLLF FKICMNASR RLHNLMFSN 
      VLQAPMRFF DTNPSGRIL NRFSKDMGA MDELLPRAT 
      IEAIQIFLV MSGILLMVF IVTPWMIAV AVVLGGLFY 
      WFRVIYLAT AQDVKRLEG ITRAPVFSH VSASLYGLP 
      TIRAASAQN MIIKEFDLL QDQHTGSWF MFIASSEAF 
      GLYLDIIST VFLAIVTYQ FLIFDDGTT KRGNVGLVI 
      SQSLILTGM LQYGVRQTA EVASNMTSV ERILQYTKL 
      DKEGPFESL PTKKPSRDW PQQGRVVFK NVYLRYIPN 
      DPPVLKNLS FEIQPGEKV GIVGRTGAG KSSLISALF 
      RLTKIEGAI EIDGVNTQF IGLNDLRSN ISIIPQEPV 
      LFSNTVRYN LDPFEKCDD KTLWKALEN VELKNAIDS 
      LDQRVSEGG SNFSAGQRQ LVCLARAIV RNNKVLVLD 
      EATANVDPA TDALIQTSI RKNFKECTV LTIAHRLNT 
      IMDSDKVLV MDAGEAVEF DHPHRLLQN QEGRFSRML 
      RETGKNLET QLKEVAKND YENKFGPIS TSDEQNDNK 
      EE
    CDS sequence
    • >TC014403 RA
      atggattca agtaaaaaa tacgaccgc ccatctccc 
      gaagagaca gcgaatcct ctttcgaaa ctgttcttt 
      gcctggacg ttgccgttt ttcaaatac ggctactcg 
      aatgacttg caactcaaa gatttgtac aattgtgct 
      aaatatgac ctttcgggc aagttagga gatgacatc 
      gaacgaaat tggcttctc gaagtcaaa tcctcgttg 
      aaaaacaat aaaaagccc agcttgctt cgaaccata 
      cgccagact ttcataact ccatactca ctcacggga 
      gtcattctc tgtttgcaa tgtgcagta ttgaaaatg 
      tttcagcct atagtatta gcatggtat attaattat 
      tacggggtt aattacaat gaccggtcg tccggtacg 
      gggtggggc cttgccact ggtgttatc ctattagcg 
      tttgtcaac atcatgatt atccatcac tgtaatttg 
      tattcgcaa agaatcgga atgcggagt cggatagcc 
      acatgctca ctcatttac agaaaatta ctcagactg 
      aaccggacc tcattgggg caagtagca gtcggaaaa 
      ttagtaaat ttgctatca aacgacgta caaagattc 
      gatattact tcaactttc ctccattat atttggatc 
      atgcctata caagcaatc attgccttt tacgtaatg 
      taccgaagt gtcgggctt gccgccctg tccggcctc 
      ggtgctata accctccaa gccgtcttc ttgcaaggc 
      tacttgagc cgacttcag gggatttac cgaatgcaa 
      atagcacaa aaaaccgac aatcgagtc aaactcatg 
      aacgagata acagccggc atacaagtc atcaaaatg 
      tacgcttgg gaaaaaccc ttcgaaaaa gtcgtagcc 
      ttagccaga aaatacgaa atcaacatc gtggccaaa 
      acctcctac attcggggt ttttcaatc gcgttgatg 
      gtcttcaca gaacgcaca gcttcgtac ttaaccctc 
      atcactttc gtacttatg ggcaacgta ttgagcgca 
      gatgtggtt ttttccgtt gcccaactt ttcaacacc 
      gttcaactc tacatgtgt atattatat cctctgggc 
      atttcgact tttgccgaa gccaaaact tcagtcaag 
      cgaattgag gagttcctg ctccttgaa gagaatgaa 
      actggggcc gacttatcg gtaacacaa tccgaaaaa 
      aatggttct atcagacta gataaagta aacgccagt 
      tggactcct aaccccatt gtgcacact ctcatggat 
      ctgaatatc gagatcaat cctgggact ttatgctgc 
      gttgtaggg ccggtaggt gcgggcaaa agctcactt 
      ttgcaactc ctcctcaag gaattaccg gcgaactca 
      ggtcgcgtt agtctcacc ggaaatata tcctatgcc 
      tcgcaagag ccttggctt ttcgtgtcc tcggtccgt 
      aataacatt cttttcgga caacaatac ttgaaaaac 
      cgctacaag gacgtggtc aaagtttgt gctttagaa 
      accgatttc gaacaattt ccacacggc gataagact 
      ctagtaggt gagagggga acctccctt agtggaggc 
      caaagagcg agggttaat ttagccaga gctgtgtac 
      agagacgcc gatatttac ctcttcgat gaccccctc 
      tccgccgtg gatgcccat gtgggcaaa cacttgttc 
      aacgaatgc atcgtgaag tatttaaaa gacaaaact 
      cgagttttg gtaacgcac caattgcag tttttgcga 
      caagctgac ctcatcata gttttaaac aatggtcaa 
      gttgagaaa atcggaact tttgccgaa ctttccgaa 
      aatgaactg aacaatcta aagttggag caacctgag 
      gaagttgcc accaaagaa caagaaatg caacaaaga 
      cagagactt atgtccatt gcgtcacac aatagcaca 
      gttcctgac gacgatgac gaaattgaa gcacaggag 
      acggaagag ttgatggaa aagggcgca atcccgact 
      tccacttac ttggattat tacagatcg ggcggaagt 
      attgcagtt ttgttgttt ttggtcgtt ttattgatt 
      atagcacag gctagttgt aatgcttcc gatctttgg 
      ttgacttat tggacaaat ttggagcaa aaatcaccg 
      gcaattgta aacggagat tcaaacacc acagaaatg 
      ccattttct ggtactaat gacacaaat acagaaccg 
      ccaactgaa ctaaacaca acagaaata atatctaca 
      gcactacca ccagtattt aatttgaca gatccaaac 
      gaacccctc tcccgagct acctatatc tgggtctac 
      tcgatcctt atcctcttc tccattatt ctgacaaca 
      agccgctca ctcctcttc tttaaaatc tgtatgaac 
      gcatccaga cgtctccat aacctaatg ttcagtaat 
      gttctccaa gccccgatg cgatttttc gatactaat 
      ccctctggt cgtatctta aaccggttc tccaaagac 
      atgggggcc atggatgaa ctcctacct cgcgctaca 
      attgaagcg attcaaatc tttttggtc atgtcgggg 
      attctatta atggttttt attgtaact ccgtggatg 
      attgctgtt gctgtagtt ctgggtggt ctgttttac 
      tggttcaga gttatctat ctcgcgaca gcacaagat 
      gttaaaaga ctggaagga ataacaaga gcgccggtt 
      ttctctcac gtgtctgct tctttgtac ggccttccg 
      acaataagg gcagcaagt gcacaaaac atgataata 
      aaagaattt gacttatta caagaccaa cacacaggg 
      agttggttc atgtttatt gcttccagt gaggcattc 
      ggactttac ctcgatata atcagtaca gtgtttttg 
      gcaattgtg acgtatcag tttcttatt tttgacgat 
      ggaacaacc aaacgtgga aacgtaggt ctagtcatt 
      tctcaaagc ctgattcta acggggatg ttacaatac 
      ggcgtccgg caaacagcg gaagttgcc agtaacatg 
      actagcgtt gaacgtatt ttgcaatac actaaactg 
      gataaagaa ggtcctttt gaatcatta cccactaaa 
      aaaccaagt cgggattgg ccacaacaa ggccgtgtc 
      gtcttcaaa aacgtgtat ctgcggtac atccctaac 
      gatccccca gttttgaaa aacctcagt ttcgaaata 
      caaccaggg gaaaaagtc ggtatcgtg gggcgcacc 
      ggcgctggc aagtcatcg ttaatatcc gcccttttt 
      cgccttacg aaaatcgaa ggagccatt gaaatcgat 
      ggagtcaac actcaattc atcggtttg aatgaccta 
      aggtccaac atttcaatc attcctcag gagccagtt 
      ctcttctcg aatacagta cgctacaat cttgaccca 
      tttgaaaaa tgtgatgac aagacttta tggaaggct 
      ttagaaaac gtcgaactc aaaaatgcc atagacagc 
      ttagaccaa agagtgagc gagggaggt tccaatttt 
      agtgccggc caacgccag cttgtctgt cttgccaga 
      gctatagtc aggaataac aaagtgctg gttctggat 
      gaagcaacc gctaatgtt gatccggca actgatgct 
      ctgatccaa accagtata cgtaaaaat ttcaaagag 
      tgtacagtc ctaactata gcacatcga ttgaatact 
      ataatggac tcggacaag gttttggtg atggatgca 
      ggagaagcg gtggagttt gaccaccca caccggctt 
      ttacaaaat caggaaggt cgttttagt cgcatgctt 
      cgcgaaacg gggaaaaat ttagagacc cagctgaaa 
      gaagttgca aaaaatgac tacgagaac aaattcgga 
      ccaataagt accagtgat gaacaaaat gataataag 
      gaagaatag
    mRNA sequence
    • >TC014403 RA
      gatgtgttt agttactta atgaatatg acttaaacc 
      gggcggcct taattgatt tgttctcgt gcctttgat 
      agtacttaa gccatggat tcaagtaaa aaatacgac 
      cgcccatct cccgaagag acagcgaat cctctttcg 
      aaactgttc tttgcctgg acgttgccg tttttcaaa 
      tacggctac tcgaatgac ttgcaactc aaagatttg 
      tacaattgt gctaaatat gacctttcg ggcaagtta 
      ggagatgac atcgaacga aattggctt ctcgaagtc 
      aaatcctcg ttgaaaaac aataaaaag cccagcttg 
      cttcgaacc atacgccag actttcata actccatac 
      tcactcacg ggagtcatt ctctgtttg caatgtgca 
      gtattgaaa atgtttcag cctatagta ttagcatgg 
      tatattaat tattacggg gttaattac aatgaccgg 
      tcgtccggt acggggtgg ggccttgcc actggtgtt 
      atcctatta gcgtttgtc aacatcatg attatccat 
      cactgtaat ttgtattcg caaagaatc ggaatgcgg 
      agtcggata gccacatgc tcactcatt tacagaaaa 
      ttactcaga ctgaaccgg acctcattg gggcaagta 
      gcagtcgga aaattagta aatttgcta tcaaacgac 
      gtacaaaga ttcgatatt acttcaact ttcctccat 
      tatatttgg atcatgcct atacaagca atcattgcc 
      ttttacgta atgtaccga agtgtcggg cttgccgcc 
      ctgtccggc ctcggtgct ataaccctc caagccgtc 
      ttcttgcaa ggctacttg agccgactt caggggatt 
      taccgaatg caaatagca caaaaaacc gacaatcga 
      gtcaaactc atgaacgag ataacagcc ggcatacaa 
      gtcatcaaa atgtacgct tgggaaaaa cccttcgaa 
      aaagtcgta gccttagcc agaaaatac gaaatcaac 
      atcgtggcc aaaacctcc tacattcgg ggtttttca 
      atcgcgttg atggtcttc acagaacgc acagcttcg 
      tacttaacc ctcatcact ttcgtactt atgggcaac 
      gtattgagc gcagatgtg gttttttcc gttgcccaa 
      cttttcaac accgttcaa ctctacatg tgtatatta 
      tatcctctg ggcatttcg acttttgcc gaagccaaa 
      acttcagtc aagcgaatt gaggagttc ctgctcctt 
      gaagagaat gaaactggg gccgactta tcggtaaca 
      caatccgaa aaaaatggt tctatcaga ctagataaa 
      gtaaacgcc agttggact cctaacccc attgtgcac 
      actctcatg gatctgaat atcgagatc aatcctggg 
      actttatgc tgcgttgta gggccggta ggtgcgggc 
      aaaagctca cttttgcaa ctcctcctc aaggaatta 
      ccggcgaac tcaggtcgc gttagtctc accggaaat 
      atatcctat gcctcgcaa gagccttgg cttttcgtg 
      tcctcggtc cgtaataac attcttttc ggacaacaa 
      tacttgaaa aaccgctac aaggacgtg gtcaaagtt 
      tgtgcttta gaaaccgat ttcgaacaa tttccacac 
      ggcgataag actctagta ggtgagagg ggaacctcc 
      cttagtgga ggccaaaga gcgagggtt aatttagcc 
      agagctgtg tacagagac gccgatatt tacctcttc 
      gatgacccc ctctccgcc gtggatgcc catgtgggc 
      aaacacttg ttcaacgaa tgcatcgtg aagtattta 
      aaagacaaa actcgagtt ttggtaacg caccaattg 
      cagtttttg cgacaagct gacctcatc atagtttta 
      aacaatggt caagttgag aaaatcgga acttttgcc 
      gaactttcc gaaaatgaa ctgaacaat ctaaagttg 
      gagcaacct gaggaagtt gccaccaaa gaacaagaa 
      atgcaacaa agacagaga cttatgtcc attgcgtca 
      cacaatagc acagttcct gacgacgat gacgaaatt 
      gaagcacag gagacggaa gagttgatg gaaaagggc 
      gcaatcccg acttccact tacttggat tattacaga 
      tcgggcgga agtattgca gttttgttg tttttggtc 
      gttttattg attatagca caggctagt tgtaatgct 
      tccgatctt tggttgact tattggaca aatttggag 
      caaaaatca ccggcaatt gtaaacgga gattcaaac 
      accacagaa atgccattt tctggtact aatgacaca 
      aatacagaa ccgccaact gaactaaac acaacagaa 
      ataatatct acagcacta ccaccagta tttaatttg 
      acagatcca aacgaaccc ctctcccga gctacctat 
      atctgggtc tactcgatc cttatcctc ttctccatt 
      attctgaca acaagccgc tcactcctc ttctttaaa 
      atctgtatg aacgcatcc agacgtctc cataaccta 
      atgttcagt aatgttctc caagccccg atgcgattt 
      ttcgatact aatccctct ggtcgtatc ttaaaccgg 
      ttctccaaa gacatgggg gccatggat gaactccta 
      cctcgcgct acaattgaa gcgattcaa atctttttg 
      gtcatgtcg gggattcta ttaatggtt tttattgta 
      actccgtgg atgattgct gttgctgta gttctgggt 
      ggtctgttt tactggttc agagttatc tatctcgcg 
      acagcacaa gatgttaaa agactggaa ggaataaca 
      agagcgccg gttttctct cacgtgtct gcttctttg 
      tacggcctt ccgacaata agggcagca agtgcacaa 
      aacatgata ataaaagaa tttgactta ttacaagac 
      caacacaca gggagttgg ttcatgttt attgcttcc 
      agtgaggca ttcggactt tacctcgat ataatcagt 
      acagtgttt ttggcaatt gtgacgtat cagtttctt 
      atttttgac gatggaaca accaaacgt ggaaacgta 
      ggtctagtc atttctcaa agcctgatt ctaacgggg 
      atgttacaa tacggcgtc cggcaaaca gcggaagtt 
      gccagtaac atgactagc gttgaacgt attttgcaa 
      tacactaaa ctggataaa gaaggtcct tttgaatca 
      ttacccact aaaaaacca agtcgggat tggccacaa 
      caaggccgt gtcgtcttc aaaaacgtg tatctgcgg 
      tacatccct aacgatccc ccagttttg aaaaacctc 
      agtttcgaa atacaacca ggggaaaaa gtcggtatc 
      gtggggcgc accggcgct ggcaagtca tcgttaata 
      tccgccctt tttcgcctt acgaaaatc gaaggagcc 
      attgaaatc gatggagtc aacactcaa ttcatcggt 
      ttgaatgac ctaaggtcc aacatttca atcattcct 
      caggagcca gttctcttc tcgaataca gtacgctac 
      aatcttgac ccatttgaa aaatgtgat gacaagact 
      ttatggaag gctttagaa aacgtcgaa ctcaaaaat 
      gccatagac agcttagac caaagagtg agcgaggga 
      ggttccaat tttagtgcc ggccaacgc cagcttgtc 
      tgtcttgcc agagctata gtcaggaat aacaaagtg 
      ctggttctg gatgaagca accgctaat gttgatccg 
      gcaactgat gctctgatc caaaccagt atacgtaaa 
      aatttcaaa gagtgtaca gtcctaact atagcacat 
      cgattgaat actataatg gactcggac aaggttttg 
      gtgatggat gcaggagaa gcggtggag tttgaccac 
      ccacaccgg cttttacaa aatcaggaa ggtcgtttt 
      agtcgcatg cttcgcgaa acggggaaa aatttagag 
      acccagctg aaagaagtt gcaaaaaat gactacgag 
      aacaaattc ggaccaata agtaccagt gatgaacaa 
      aatgataat aaggaagaa tagaagatt tattttttt 
      aaaacttaa taaataatt gagacgctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY