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Gene information
General information
TC014386 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9983363..9987536
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014386
    iBeetle screen iB_02282

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late potentiallyshape irregular - Number of animals showing the phenotype: 2

    mature egg not present - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014386 RA
      MDHGETQQR KTHPKERTN FISNIFFIY AIKLFKKGF 
      TRDLEESDL YDVLSDYKS KKLGDQLEL EWEKQKKRR 
      KNLSIVRLL LSCYGFPYL LLGFMQLFI NVVEIIVQP 
      YALGKLVLY FSPDQTELT KTDAYYYAA IVIGLNFFQ 
      RVYKHNYYL LLAGFGIKT RAAFCSFMY RKALKLSPA 
      HMEDISIGK IVTLITKDV DTFELFIDF GNDIWIGIV 
      KTIVVSVIF YIRIGWSAF SGVGFFLLV LPLQVWLGS 
      KITAMKMNM CKKTDERLQ MTQETLSAI RIIKMYTWE 
      KIFDGKISQ ARKKEVYTT YKIFFVRFL IIVIGSLNS 
      HIAFYLILM TYIWCGNVI TAEIVYFIM GTFQNLSYG 
      LAILFPIGV YQTAELKSS IKRIGQIMK AAEIQSEKI 
      PNTEITILP KINMKNVTV SVRDAKILQ NVTLNIEKG 
      LTLLTGPVG SGKSFLLKT ILQDYQPEK GNLMVQGTV 
      SYASQEPWL FPSSIKQNI LFGQKYNEK RYNEVLKVC 
      ALIYDFELL EAGDNTIVE DRGINLSKG QQARINLAR 
      AVYKESDIY LLDDSLAAL DAHVSSFIF KECVLGFLK 
      DKLVIMVSH NVDYVKDSD VIIVMKNGQ ITQSGKLSE 
      LNTNELLET VEEKKENEV CDDETGEEE AATEETKLM 
      TDAPVQRKV YQEQKQSGA VKTAVYNKY IKFGGGYFV 
      LFLVFCIFV SAQITMSYT DKLVSDWVN LEQKISNFT 
      IQNATNTTE QVELYSQKN YIFYLYTFM TIATGVTSL 
      SRAIGLLWF SRSAALKLH KNMITTVIN ASMQFFDTN 
      FIGNILNRF SKDLITVDE SILFAFYHV FRVLLVIIG 
      IVALIAGVN PMFLIPTAI FLLILLVLR RFCLRTSRS 
      LKRLDALTR SPVVGHLNA SLEGLTTIR AFQAEEILR 
      DEFDRHQDL YTSASYILQ SSMRAFAFT MDTLCSFYI 
      SFIVVKFLF TDDDVLAGH VGLAISQAF NLTGTLQWG 
      IRQWAEMEN RMTSVERVL EYTEIKKEN KQGLELDNW 
      PSVGMVKYE NVCLTYTNS NEQVLKNIN FVANPKEKI 
      GIVGRTGAG KSSIISTLF RLYEVEGKI LIDGVDTKT 
      VSLDCLRAN ISIIPQDPV LFTGTIREN IDPTHRYKD 
      DEIWKAIET AHLKKLIPS LDFEIVEGG SNFSIGQRQ 
      LICLARAVI QKNKIIVMD EATANMDPE TDALIHKTM 
      EESFKECTV FTIAHKLQS ILRSDKVMV MDKGEIVEF 
      DEPTNLLEN KDGIFYKMV AKAGLLPQK
    CDS sequence
    • >TC014386 RA
      atggatcac ggggaaaca cagcagcgt aaaactcac 
      cccaaggaa cgaaccaac tttatttca aacatattc 
      tttatttac gccatcaaa cttttcaaa aaagggttc 
      acacgagac ttggaagag agcgaccta tacgacgtt 
      ctttcagac tacaaatcg aagaaacta ggggaccaa 
      ctcgaacta gaatgggag aaacagaag aagcggcgg 
      aaaaatcta tcaatagtt cgcctcctg ttaagttgt 
      tacgggttc ccgtacctc cttctaggt tttatgcag 
      ttgttcatc aacgtggta gaaataata gtccagccg 
      tatgctttg ggcaaacta gttttgtat ttctcgcca 
      gaccaaacc gaacttacg aagacagac gcttactac 
      tatgctgcc atagtcatt ggactgaac ttcttccag 
      cgggtgtac aagcacaac tattacctc ctcctggcc 
      ggatttggg atcaaaact cgggccgcc ttctgctcc 
      tttatgtac agaaaggcg ctgaagctg agcccggcc 
      cacatggag gacatctcg atcgggaaa atcgtgact 
      ctgattacc aaagatgtc gacacgttt gaacttttc 
      atcgacttc ggcaacgac atttggatc ggaatcgtc 
      aaaacgatc gtggttagt gtgattttt tacatcagg 
      atcggctgg tcggcgttc tccggagtc gggtttttc 
      ctcctagtt ttgccccta caagtgtgg ctcggttca 
      aaaatcacc gccatgaag atgaacatg tgtaagaaa 
      accgatgaa aggctacaa atgacacaa gaaactcta 
      tcagctatt aggattatc aaaatgtac acttgggaa 
      aagattttt gacggcaag atttcccaa gccagaaag 
      aaagaagtc tacacgact tacaagatc ttctttgtg 
      agatttttg atcatagtg attgggagt ttaaactcc 
      cacatagct ttttattta attctgatg acctacatc 
      tggtgtggt aatgtcata accgcggaa atcgtctac 
      tttatcatg ggcacgttc caaaatctg tcctacggt 
      ctggccatc ctgttcccc atcggcgtc taccaaacc 
      gctgaactt aaatcgtca atcaagcgg attggtcaa 
      atcatgaaa gcggcagaa attcaatca gagaaaatc 
      cccaacacc gaaatcaca attttgccc aaaatcaac 
      atgaaaaat gttactgtc tcagtccga gatgccaaa 
      atcctgcaa aacgttact ctgaatatt gaaaaaggg 
      ctaactctc ctcactggt cctgtagga agcggcaag 
      tcattcctc ttgaagacg atcctgcaa gactaccaa 
      cctgaaaag ggaaacttg atggtccaa ggcaccgtg 
      tcttacgcc tcccaagaa ccctggctt ttcccgtcc 
      tcaatcaaa caaaacatt ttattcggc caaaaatac 
      aacgaaaag cgctacaac gaagtgctg aaagtgtgc 
      gctttgatc tacgacttc gaactattg gaagccggt 
      gacaacacc atagtggag gatcgtgga ataaattta 
      agtaaagga caacaggcc cgcattaac ctagcgcga 
      gccgtctac aaggaaagc gacatttac ctcctggac 
      gactctctg gcggctttg gacgcccac gtgagctcg 
      tttattttc aaagagtgt gtcttgggc ttcctcaaa 
      gacaaactg gtgatcatg gtcagtcat aacgtcgac 
      tatgtgaaa gacagcgat gttatcatc gttatgaaa 
      aatgggcaa atcacgcaa agtggaaaa ctgtccgaa 
      cttaacacc aacgagctg ctggaaacc gtcgaagag 
      aaaaaggaa aatgaagta tgtgacgat gaaaccggc 
      gaagaggaa gcagcaact gaagaaacc aaactgatg 
      accgacgcg cctgtccaa agaaaagtc taccaagaa 
      cagaagcaa tctggagcc gttaaaaca gccgtctac 
      aacaagtat atcaaattc ggtggtggc tacttcgtt 
      ctgtttcta gttttttgt attttcgtg tcggcgcaa 
      atcacgatg agttacacg gacaagtta gtgagtgac 
      tgggtcaat ttggaacaa aaaatttcc aacttcact 
      atacaaaat gccacgaat accaccgaa caggtggaa 
      ctctacagt cagaaaaac tacattttc tacttgtat 
      acatttatg accatagcc acgggggtt actagtctg 
      agtcgagca attgggctt ttgtggttc tcacggagt 
      gcggccctc aagctgcac aaaaacatg atcacgact 
      gttattaac gcatcaatg cagttcttc gacacgaat 
      ttcatcgga aatatcttg aacaggttc tcgaaggat 
      ttgattaca gtcgacgag tcgatttta ttcgctttt 
      taccacgtc ttccgagtc cttttggtc ataattggg 
      attgtggca ctgatagct ggtgttaac cccatgttt 
      ttgatcccc actgccata tttctcttg atactgttg 
      gttttgaga aggttttgt cttcgaact tccaggagt 
      ttgaaacga ttggatgct ttgactagg agtccagtt 
      gttggccac cttaacgct tcgcttgaa ggcttgacc 
      acaatccgg gcgttccaa gcggaagaa attctcaga 
      gatgagttc gaccgtcac caggatctc tacacttct 
      gcttcctat attcttcaa agcagcatg agggcgttt 
      gcgttcaca atggacact ttgtgcagt ttctacatt 
      tcgtttatt gttgtcaaa tttttgttt accgatgat 
      gatgttttg gccggtcat gttggtctc gccatatca 
      caagccttt aatctgact gggacttta caatggggc 
      attcgccag tgggccgaa atggaaaac cgaatgact 
      tcggtcgaa agagtattg gaatatacc gaaattaaa 
      aaagaaaac aaacaaggc ttggaatta gacaactgg 
      ccaagtgtg ggaatggtc aagtatgag aacgtttgc 
      ctgacttat acgaattcg aacgaacaa gtcttaaaa 
      aatatcaac ttcgtggct aatcccaaa gagaagatt 
      gggattgtc ggtcgaacc ggtgccgga aaatcgtcg 
      attatttcc accttgttc cggttatac gaagtcgag 
      gggaagatt ctgatcgac ggtgtggac acgaagacc 
      gtctcgctt gactgcctc agagccaac atttccatc 
      atcccgcag gaccccgtc ctgttcacg ggaaccatc 
      cgcgaaaac atcgaccct acccatcga tacaaagac 
      gacgagatc tggaaagcc atcgaaacg gcccacttg 
      aagaaactc atcccgagc ctagacttt gagatcgtt 
      gaaggaggc tcgaatttc agtataggg caaagacag 
      ttgatttgt ctcgccaga gccgtcatc cagaagaac 
      aagatcatc gtcatggac gaagccacg gctaacatg 
      gacccggaa acggacgcc ttgatccac aaaaccatg 
      gaggagagt ttcaaggag tgtaccgtt ttcacgatc 
      gctcacaag ttgcagtcg attttgcga agtgataaa 
      gttatggtt atggacaag ggcgagatt gttgagttt 
      gacgaaccg acgaattta ttggaaaat aaggacggg 
      atcttctac aaaatggtg gcgaaggcg ggactgctg 
      ccgcagaaa tag
    mRNA sequence
    • >TC014386 RA
      aagtacgac tttcaaaat ggatcacgg ggaaacaca 
      gcagcgtaa aactcaccc caaggaacg aaccaactt 
      tatttcaaa catattctt tatttacgc catcaaact 
      tttcaaaaa agggttcac acgagactt ggaagagag 
      cgacctata cgacgttct ttcagacta caaatcgaa 
      gaaactagg ggaccaact cgaactaga atgggagaa 
      acagaagaa gcggcggaa aaatctatc aatagttcg 
      cctcctgtt aagttgtta cgggttccc gtacctcct 
      tctaggttt tatgcagtt gttcatcaa cgtggtaga 
      aataatagt ccagccgta tgctttggg caaactagt 
      tttgtattt ctcgccaga ccaaaccga acttacgaa 
      gacagacgc ttactacta tgctgccat agtcattgg 
      actgaactt cttccagcg ggtgtacaa gcacaacta 
      ttacctcct cctggccgg atttgggat caaaactcg 
      ggccgcctt ctgctcctt tatgtacag aaaggcgct 
      gaagctgag cccggccca catggagga catctcgat 
      cgggaaaat cgtgactct gattaccaa agatgtcga 
      cacgtttga acttttcat cgacttcgg caacgacat 
      ttggatcgg aatcgtcaa aacgatcgt ggttagtgt 
      gatttttta catcaggat cggctggtc ggcgttctc 
      cggagtcgg gtttttcct cctagtttt gcccctaca 
      agtgtggct cggttcaaa aatcaccgc catgaagat 
      gaacatgtg taagaaaac cgatgaaag gctacaaat 
      gacacaaga aactctatc agctattag gattatcaa 
      aatgtacac ttgggaaaa gatttttga cggcaagat 
      ttcccaagc cagaaagaa agaagtcta cacgactta 
      caagatctt ctttgtgag atttttgat catagtgat 
      tgggagttt aaactccca catagcttt ttatttaat 
      tctgatgac ctacatctg gtgtggtaa tgtcataac 
      cgcggaaat cgtctactt tatcatggg cacgttcca 
      aaatctgtc ctacggtct ggccatcct gttccccat 
      cggcgtcta ccaaaccgc tgaacttaa atcgtcaat 
      caagcggat tggtcaaat catgaaagc ggcagaaat 
      tcaatcaga gaaaatccc caacaccga aatcacaat 
      tttgcccaa aatcaacat gaaaaatgt tactgtctc 
      agtccgaga tgccaaaat cctgcaaaa cgttactct 
      gaatattga aaaagggct aactctcct cactggtcc 
      tgtaggaag cggcaagtc attcctctt gaagacgat 
      cctgcaaga ctaccaacc tgaaaaggg aaacttgat 
      ggtccaagg caccgtgtc ttacgcctc ccaagaacc 
      ctggctttt cccgtcctc aatcaaaca aaacatttt 
      attcggcca aaaatacaa cgaaaagcg ctacaacga 
      agtgctgaa agtgtgcgc tttgatcta cgacttcga 
      actattgga agccggtga caacaccat agtggagga 
      tcgtggaat aaatttaag taaaggaca acaggcccg 
      cattaacct agcgcgagc cgtctacaa ggaaagcga 
      catttacct cctggacga ctctctggc ggctttgga 
      cgcccacgt gagctcgtt tattttcaa agagtgtgt 
      cttgggctt cctcaaaga caaactggt gatcatggt 
      cagtcataa cgtcgacta tgtgaaaga cagcgatgt 
      tatcatcgt tatgaaaaa tgggcaaat cacgcaaag 
      tggaaaact gtccgaact taacaccaa cgagctgct 
      ggaaaccgt cgaagagaa aaaggaaaa tgaagtatg 
      tgacgatga aaccggcga agaggaagc agcaactga 
      agaaaccaa actgatgac cgacgcgcc tgtccaaag 
      aaaagtcta ccaagaaca gaagcaatc tggagccgt 
      taaaacagc cgtctacaa caagtatat caaattcgg 
      tggtggcta cttcgttct gtttctagt tttttgtat 
      tttcgtgtc ggcgcaaat cacgatgag ttacacgga 
      caagttagt gagtgactg ggtcaattt ggaacaaaa 
      aatttccaa cttcactat acaaaatgc cacgaatac 
      caccgaaca ggtggaact ctacagtca gaaaaacta 
      cattttcta cttgtatac atttatgac catagccac 
      gggggttac tagtctgag tcgagcaat tgggctttt 
      gtggttctc acggagtgc ggccctcaa gctgcacaa 
      aaacatgat cacgactgt tattaacgc atcaatgca 
      gttcttcga cacgaattt catcggaaa tatcttgaa 
      caggttctc gaaggattt gattacagt cgacgagtc 
      gattttatt cgcttttta ccacgtctt ccgagtcct 
      tttggtcat aattgggat tgtggcact gatagctgg 
      tgttaaccc catgttttt gatccccac tgccatatt 
      tctcttgat actgttggt tttgagaag gttttgtct 
      tcgaacttc caggagttt gaaacgatt ggatgcttt 
      gactaggag tccagttgt tggccacct taacgcttc 
      gcttgaagg cttgaccac aatccgggc gttccaagc 
      ggaagaaat tctcagaga tgagttcga ccgtcacca 
      ggatctcta cacttctgc ttcctatat tcttcaaag 
      cagcatgag ggcgtttgc gttcacaat ggacacttt 
      gtgcagttt ctacatttc gtttattgt tgtcaaatt 
      tttgtttac cgatgatga tgttttggc cggtcatgt 
      tggtctcgc catatcaca agcctttaa tctgactgg 
      gactttaca atggggcat tcgccagtg ggccgaaat 
      ggaaaaccg aatgacttc ggtcgaaag agtattgga 
      atataccga aattaaaaa agaaaacaa acaaggctt 
      ggaattaga caactggcc aagtgtggg aatggtcaa 
      gtatgagaa cgtttgcct gacttatac gaattcgaa 
      cgaacaagt cttaaaaaa tatcaactt cgtggctaa 
      tcccaaaga gaagattgg gattgtcgg tcgaaccgg 
      tgccggaaa atcgtcgat tatttccac cttgttccg 
      gttatacga agtcgaggg gaagattct gatcgacgg 
      tgtggacac gaagaccgt ctcgcttga ctgcctcag 
      agccaacat ttccatcat cccgcagga ccccgtcct 
      gttcacggg aaccatccg cgaaaacat cgaccctac 
      ccatcgata caaagacga cgagatctg gaaagccat 
      cgaaacggc ccacttgaa gaaactcat cccgagcct 
      agactttga gatcgttga aggaggctc gaatttcag 
      tatagggca aagacagtt gatttgtct cgccagagc 
      cgtcatcca gaagaacaa gatcatcgt catggacga 
      agccacggc taacatgga cccggaaac ggacgcctt 
      gatccacaa aaccatgga ggagagttt caaggagtg 
      taccgtttt cacgatcgc tcacaagtt gcagtcgat 
      tttgcgaag tgataaagt tatggttat ggacaaggg 
      cgagattgt tgagtttga cgaaccgac gaatttatt 
      ggaaaataa ggacgggat cttctacaa aatggtggc 
      gaaggcggg actgctgcc gcagaaata gctaataaa 
      aagtgtaca gcaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY