Status
Please wait ...
Gene information
General information
TC014386 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9983363..9987536
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014386
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02282

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late potentiallyshape irregular - Number of animals showing the phenotype: 2

    mature egg not present - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014386 RA
      MDHGETQQR KTHPKERTN FISNIFFIY AIKLFKKGF 
TRDLEESDL YDVLSDYKS KKLGDQLEL EWEKQKKRR 
KNLSIVRLL LSCYGFPYL LLGFMQLFI NVVEIIVQP 
YALGKLVLY FSPDQTELT KTDAYYYAA IVIGLNFFQ 
RVYKHNYYL LLAGFGIKT RAAFCSFMY RKALKLSPA 
HMEDISIGK IVTLITKDV DTFELFIDF GNDIWIGIV 
KTIVVSVIF YIRIGWSAF SGVGFFLLV LPLQVWLGS 
KITAMKMNM CKKTDERLQ MTQETLSAI RIIKMYTWE 
KIFDGKISQ ARKKEVYTT YKIFFVRFL IIVIGSLNS 
HIAFYLILM TYIWCGNVI TAEIVYFIM GTFQNLSYG 
LAILFPIGV YQTAELKSS IKRIGQIMK AAEIQSEKI 
PNTEITILP KINMKNVTV SVRDAKILQ NVTLNIEKG 
LTLLTGPVG SGKSFLLKT ILQDYQPEK GNLMVQGTV 
SYASQEPWL FPSSIKQNI LFGQKYNEK RYNEVLKVC 
ALIYDFELL EAGDNTIVE DRGINLSKG QQARINLAR 
AVYKESDIY LLDDSLAAL DAHVSSFIF KECVLGFLK 
DKLVIMVSH NVDYVKDSD VIIVMKNGQ ITQSGKLSE 
LNTNELLET VEEKKENEV CDDETGEEE AATEETKLM 
TDAPVQRKV YQEQKQSGA VKTAVYNKY IKFGGGYFV 
LFLVFCIFV SAQITMSYT DKLVSDWVN LEQKISNFT 
IQNATNTTE QVELYSQKN YIFYLYTFM TIATGVTSL 
SRAIGLLWF SRSAALKLH KNMITTVIN ASMQFFDTN 
FIGNILNRF SKDLITVDE SILFAFYHV FRVLLVIIG 
IVALIAGVN PMFLIPTAI FLLILLVLR RFCLRTSRS 
LKRLDALTR SPVVGHLNA SLEGLTTIR AFQAEEILR 
DEFDRHQDL YTSASYILQ SSMRAFAFT MDTLCSFYI 
SFIVVKFLF TDDDVLAGH VGLAISQAF NLTGTLQWG 
IRQWAEMEN RMTSVERVL EYTEIKKEN KQGLELDNW 
PSVGMVKYE NVCLTYTNS NEQVLKNIN FVANPKEKI 
GIVGRTGAG KSSIISTLF RLYEVEGKI LIDGVDTKT 
VSLDCLRAN ISIIPQDPV LFTGTIREN IDPTHRYKD 
DEIWKAIET AHLKKLIPS LDFEIVEGG SNFSIGQRQ 
LICLARAVI QKNKIIVMD EATANMDPE TDALIHKTM 
EESFKECTV FTIAHKLQS ILRSDKVMV MDKGEIVEF 
DEPTNLLEN KDGIFYKMV AKAGLLPQK
    CDS sequence
    • >TC014386 RA
      atggatcac ggggaaaca cagcagcgt aaaactcac 
cccaaggaa cgaaccaac tttatttca aacatattc 
tttatttac gccatcaaa cttttcaaa aaagggttc 
acacgagac ttggaagag agcgaccta tacgacgtt 
ctttcagac tacaaatcg aagaaacta ggggaccaa 
ctcgaacta gaatgggag aaacagaag aagcggcgg 
aaaaatcta tcaatagtt cgcctcctg ttaagttgt 
tacgggttc ccgtacctc cttctaggt tttatgcag 
ttgttcatc aacgtggta gaaataata gtccagccg 
tatgctttg ggcaaacta gttttgtat ttctcgcca 
gaccaaacc gaacttacg aagacagac gcttactac 
tatgctgcc atagtcatt ggactgaac ttcttccag 
cgggtgtac aagcacaac tattacctc ctcctggcc 
ggatttggg atcaaaact cgggccgcc ttctgctcc 
tttatgtac agaaaggcg ctgaagctg agcccggcc 
cacatggag gacatctcg atcgggaaa atcgtgact 
ctgattacc aaagatgtc gacacgttt gaacttttc 
atcgacttc ggcaacgac atttggatc ggaatcgtc 
aaaacgatc gtggttagt gtgattttt tacatcagg 
atcggctgg tcggcgttc tccggagtc gggtttttc 
ctcctagtt ttgccccta caagtgtgg ctcggttca 
aaaatcacc gccatgaag atgaacatg tgtaagaaa 
accgatgaa aggctacaa atgacacaa gaaactcta 
tcagctatt aggattatc aaaatgtac acttgggaa 
aagattttt gacggcaag atttcccaa gccagaaag 
aaagaagtc tacacgact tacaagatc ttctttgtg 
agatttttg atcatagtg attgggagt ttaaactcc 
cacatagct ttttattta attctgatg acctacatc 
tggtgtggt aatgtcata accgcggaa atcgtctac 
tttatcatg ggcacgttc caaaatctg tcctacggt 
ctggccatc ctgttcccc atcggcgtc taccaaacc 
gctgaactt aaatcgtca atcaagcgg attggtcaa 
atcatgaaa gcggcagaa attcaatca gagaaaatc 
cccaacacc gaaatcaca attttgccc aaaatcaac 
atgaaaaat gttactgtc tcagtccga gatgccaaa 
atcctgcaa aacgttact ctgaatatt gaaaaaggg 
ctaactctc ctcactggt cctgtagga agcggcaag 
tcattcctc ttgaagacg atcctgcaa gactaccaa 
cctgaaaag ggaaacttg atggtccaa ggcaccgtg 
tcttacgcc tcccaagaa ccctggctt ttcccgtcc 
tcaatcaaa caaaacatt ttattcggc caaaaatac 
aacgaaaag cgctacaac gaagtgctg aaagtgtgc 
gctttgatc tacgacttc gaactattg gaagccggt 
gacaacacc atagtggag gatcgtgga ataaattta 
agtaaagga caacaggcc cgcattaac ctagcgcga 
gccgtctac aaggaaagc gacatttac ctcctggac 
gactctctg gcggctttg gacgcccac gtgagctcg 
tttattttc aaagagtgt gtcttgggc ttcctcaaa 
gacaaactg gtgatcatg gtcagtcat aacgtcgac 
tatgtgaaa gacagcgat gttatcatc gttatgaaa 
aatgggcaa atcacgcaa agtggaaaa ctgtccgaa 
cttaacacc aacgagctg ctggaaacc gtcgaagag 
aaaaaggaa aatgaagta tgtgacgat gaaaccggc 
gaagaggaa gcagcaact gaagaaacc aaactgatg 
accgacgcg cctgtccaa agaaaagtc taccaagaa 
cagaagcaa tctggagcc gttaaaaca gccgtctac 
aacaagtat atcaaattc ggtggtggc tacttcgtt 
ctgtttcta gttttttgt attttcgtg tcggcgcaa 
atcacgatg agttacacg gacaagtta gtgagtgac 
tgggtcaat ttggaacaa aaaatttcc aacttcact 
atacaaaat gccacgaat accaccgaa caggtggaa 
ctctacagt cagaaaaac tacattttc tacttgtat 
acatttatg accatagcc acgggggtt actagtctg 
agtcgagca attgggctt ttgtggttc tcacggagt 
gcggccctc aagctgcac aaaaacatg atcacgact 
gttattaac gcatcaatg cagttcttc gacacgaat 
ttcatcgga aatatcttg aacaggttc tcgaaggat 
ttgattaca gtcgacgag tcgatttta ttcgctttt 
taccacgtc ttccgagtc cttttggtc ataattggg 
attgtggca ctgatagct ggtgttaac cccatgttt 
ttgatcccc actgccata tttctcttg atactgttg 
gttttgaga aggttttgt cttcgaact tccaggagt 
ttgaaacga ttggatgct ttgactagg agtccagtt 
gttggccac cttaacgct tcgcttgaa ggcttgacc 
acaatccgg gcgttccaa gcggaagaa attctcaga 
gatgagttc gaccgtcac caggatctc tacacttct 
gcttcctat attcttcaa agcagcatg agggcgttt 
gcgttcaca atggacact ttgtgcagt ttctacatt 
tcgtttatt gttgtcaaa tttttgttt accgatgat 
gatgttttg gccggtcat gttggtctc gccatatca 
caagccttt aatctgact gggacttta caatggggc 
attcgccag tgggccgaa atggaaaac cgaatgact 
tcggtcgaa agagtattg gaatatacc gaaattaaa 
aaagaaaac aaacaaggc ttggaatta gacaactgg 
ccaagtgtg ggaatggtc aagtatgag aacgtttgc 
ctgacttat acgaattcg aacgaacaa gtcttaaaa 
aatatcaac ttcgtggct aatcccaaa gagaagatt 
gggattgtc ggtcgaacc ggtgccgga aaatcgtcg 
attatttcc accttgttc cggttatac gaagtcgag 
gggaagatt ctgatcgac ggtgtggac acgaagacc 
gtctcgctt gactgcctc agagccaac atttccatc 
atcccgcag gaccccgtc ctgttcacg ggaaccatc 
cgcgaaaac atcgaccct acccatcga tacaaagac 
gacgagatc tggaaagcc atcgaaacg gcccacttg 
aagaaactc atcccgagc ctagacttt gagatcgtt 
gaaggaggc tcgaatttc agtataggg caaagacag 
ttgatttgt ctcgccaga gccgtcatc cagaagaac 
aagatcatc gtcatggac gaagccacg gctaacatg 
gacccggaa acggacgcc ttgatccac aaaaccatg 
gaggagagt ttcaaggag tgtaccgtt ttcacgatc 
gctcacaag ttgcagtcg attttgcga agtgataaa 
gttatggtt atggacaag ggcgagatt gttgagttt 
gacgaaccg acgaattta ttggaaaat aaggacggg 
atcttctac aaaatggtg gcgaaggcg ggactgctg 
ccgcagaaa tag
    mRNA sequence
    • >TC014386 RA
      aagtacgac tttcaaaat ggatcacgg ggaaacaca 
gcagcgtaa aactcaccc caaggaacg aaccaactt 
tatttcaaa catattctt tatttacgc catcaaact 
tttcaaaaa agggttcac acgagactt ggaagagag 
cgacctata cgacgttct ttcagacta caaatcgaa 
gaaactagg ggaccaact cgaactaga atgggagaa 
acagaagaa gcggcggaa aaatctatc aatagttcg 
cctcctgtt aagttgtta cgggttccc gtacctcct 
tctaggttt tatgcagtt gttcatcaa cgtggtaga 
aataatagt ccagccgta tgctttggg caaactagt 
tttgtattt ctcgccaga ccaaaccga acttacgaa 
gacagacgc ttactacta tgctgccat agtcattgg 
actgaactt cttccagcg ggtgtacaa gcacaacta 
ttacctcct cctggccgg atttgggat caaaactcg 
ggccgcctt ctgctcctt tatgtacag aaaggcgct 
gaagctgag cccggccca catggagga catctcgat 
cgggaaaat cgtgactct gattaccaa agatgtcga 
cacgtttga acttttcat cgacttcgg caacgacat 
ttggatcgg aatcgtcaa aacgatcgt ggttagtgt 
gatttttta catcaggat cggctggtc ggcgttctc 
cggagtcgg gtttttcct cctagtttt gcccctaca 
agtgtggct cggttcaaa aatcaccgc catgaagat 
gaacatgtg taagaaaac cgatgaaag gctacaaat 
gacacaaga aactctatc agctattag gattatcaa 
aatgtacac ttgggaaaa gatttttga cggcaagat 
ttcccaagc cagaaagaa agaagtcta cacgactta 
caagatctt ctttgtgag atttttgat catagtgat 
tgggagttt aaactccca catagcttt ttatttaat 
tctgatgac ctacatctg gtgtggtaa tgtcataac 
cgcggaaat cgtctactt tatcatggg cacgttcca 
aaatctgtc ctacggtct ggccatcct gttccccat 
cggcgtcta ccaaaccgc tgaacttaa atcgtcaat 
caagcggat tggtcaaat catgaaagc ggcagaaat 
tcaatcaga gaaaatccc caacaccga aatcacaat 
tttgcccaa aatcaacat gaaaaatgt tactgtctc 
agtccgaga tgccaaaat cctgcaaaa cgttactct 
gaatattga aaaagggct aactctcct cactggtcc 
tgtaggaag cggcaagtc attcctctt gaagacgat 
cctgcaaga ctaccaacc tgaaaaggg aaacttgat 
ggtccaagg caccgtgtc ttacgcctc ccaagaacc 
ctggctttt cccgtcctc aatcaaaca aaacatttt 
attcggcca aaaatacaa cgaaaagcg ctacaacga 
agtgctgaa agtgtgcgc tttgatcta cgacttcga 
actattgga agccggtga caacaccat agtggagga 
tcgtggaat aaatttaag taaaggaca acaggcccg 
cattaacct agcgcgagc cgtctacaa ggaaagcga 
catttacct cctggacga ctctctggc ggctttgga 
cgcccacgt gagctcgtt tattttcaa agagtgtgt 
cttgggctt cctcaaaga caaactggt gatcatggt 
cagtcataa cgtcgacta tgtgaaaga cagcgatgt 
tatcatcgt tatgaaaaa tgggcaaat cacgcaaag 
tggaaaact gtccgaact taacaccaa cgagctgct 
ggaaaccgt cgaagagaa aaaggaaaa tgaagtatg 
tgacgatga aaccggcga agaggaagc agcaactga 
agaaaccaa actgatgac cgacgcgcc tgtccaaag 
aaaagtcta ccaagaaca gaagcaatc tggagccgt 
taaaacagc cgtctacaa caagtatat caaattcgg 
tggtggcta cttcgttct gtttctagt tttttgtat 
tttcgtgtc ggcgcaaat cacgatgag ttacacgga 
caagttagt gagtgactg ggtcaattt ggaacaaaa 
aatttccaa cttcactat acaaaatgc cacgaatac 
caccgaaca ggtggaact ctacagtca gaaaaacta 
cattttcta cttgtatac atttatgac catagccac 
gggggttac tagtctgag tcgagcaat tgggctttt 
gtggttctc acggagtgc ggccctcaa gctgcacaa 
aaacatgat cacgactgt tattaacgc atcaatgca 
gttcttcga cacgaattt catcggaaa tatcttgaa 
caggttctc gaaggattt gattacagt cgacgagtc 
gattttatt cgcttttta ccacgtctt ccgagtcct 
tttggtcat aattgggat tgtggcact gatagctgg 
tgttaaccc catgttttt gatccccac tgccatatt 
tctcttgat actgttggt tttgagaag gttttgtct 
tcgaacttc caggagttt gaaacgatt ggatgcttt 
gactaggag tccagttgt tggccacct taacgcttc 
gcttgaagg cttgaccac aatccgggc gttccaagc 
ggaagaaat tctcagaga tgagttcga ccgtcacca 
ggatctcta cacttctgc ttcctatat tcttcaaag 
cagcatgag ggcgtttgc gttcacaat ggacacttt 
gtgcagttt ctacatttc gtttattgt tgtcaaatt 
tttgtttac cgatgatga tgttttggc cggtcatgt 
tggtctcgc catatcaca agcctttaa tctgactgg 
gactttaca atggggcat tcgccagtg ggccgaaat 
ggaaaaccg aatgacttc ggtcgaaag agtattgga 
atataccga aattaaaaa agaaaacaa acaaggctt 
ggaattaga caactggcc aagtgtggg aatggtcaa 
gtatgagaa cgtttgcct gacttatac gaattcgaa 
cgaacaagt cttaaaaaa tatcaactt cgtggctaa 
tcccaaaga gaagattgg gattgtcgg tcgaaccgg 
tgccggaaa atcgtcgat tatttccac cttgttccg 
gttatacga agtcgaggg gaagattct gatcgacgg 
tgtggacac gaagaccgt ctcgcttga ctgcctcag 
agccaacat ttccatcat cccgcagga ccccgtcct 
gttcacggg aaccatccg cgaaaacat cgaccctac 
ccatcgata caaagacga cgagatctg gaaagccat 
cgaaacggc ccacttgaa gaaactcat cccgagcct 
agactttga gatcgttga aggaggctc gaatttcag 
tatagggca aagacagtt gatttgtct cgccagagc 
cgtcatcca gaagaacaa gatcatcgt catggacga 
agccacggc taacatgga cccggaaac ggacgcctt 
gatccacaa aaccatgga ggagagttt caaggagtg 
taccgtttt cacgatcgc tcacaagtt gcagtcgat 
tttgcgaag tgataaagt tatggttat ggacaaggg 
cgagattgt tgagtttga cgaaccgac gaatttatt 
ggaaaataa ggacgggat cttctacaa aatggtggc 
gaaggcggg actgctgcc gcagaaata gctaataaa 
aagtgtaca gcaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY