Status
Please wait ...
Gene information
General information
TC014378 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 2012526..2017161
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014378
    iBeetle screen iB_02281
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014378 RA
      MSDSDPYVQ KHQQLLRDL KNTVEGLLV SQVANVWSI 
      YGGLNRLHC SVEKIFKHG CKSPGIEST SYFDFIQGL 
      EWLQPENSK SYFSIDCEY KPHLPQNLK DDKASIWLY 
      RSLENHSLS QKLSWLLSD KTHLLKCYQ EYAFLSQEK 
      YAEAALICL RAVERNQAS LMSEINPCL FLLEANAKE 
      FHKMHRRCS SYPDDHFKK DYSGGDKKP KGPPTKTPV 
      IRGKLKPWH SMPSLRSDS FKIKSKQMF TQSKTTPST 
      PLYTKKIST SALKIDYKS MQYPKVRKS NLKKGFSKA 
      KKETRHVII NNCDIIEHT PPLSSSQSS GGAMGNEDL 
      MNLSTKSLP ETRNLAHSP RLVDSFLPL PGEKDYRKA 
      PKKSFIEDG GMSVLPMAT GYFPRPVKG QSLLSFLTS 
      SQFARANAE LDRENAHFS ISEAIISAM EQIRCRRDF 
      KLTDDQFDD SDPEIMDLK QRIRLRRRE KMVEKQKKV 
      WSESLLSDG RTDTATTVS PCSTTPDTL SESASSDEV 
      EDFDIDEAS NLSEDHGLS ASMASLYSE ADVLKRPRG 
      APDGASDVF SAEGVALSL ISKFNEKQL PRASDLEWL 
      VSEEDAPQA LLPLPKSWP VSPDDSETT STLLRGTQE 
      WAPPRPQII FTIHPPPTR KTLIAKQNY RCAGCSMKV 
      AQQYASRYR YCDYLGRYF CTGCHTNQL ALIPGRVIQ 
      KWDFTRYPV SSFSYRLLE QMYSDPLFR VFELNKHIW 
      KMSKNLQLC RRFRLGLFY LKDFIFACR FAESIRERL 
      EQEKSYLLT DPEVYSMED LVNVRNGDM KLRLKYLIE 
      VCCRHTSEC KLCVARGFI CEICNAPEV IFPWQMRKV 
      SRCNQCGTC YHISCWNPS DKPCNKCLR ISKRRESIG 
      NSSHDNF
    CDS sequence
    • >TC014378 RA
      atgtccgac tcggacccg tacgtgcaa aaacaccag 
      caactatta cgcgactta aaaaacaca gttgagggg 
      ctgttggtg tctcaagtg gctaacgtg tggtcaatt 
      tacggtggt ttgaaccgt ctgcactgc tccgtggag 
      aaaatattc aaacacggg tgcaaatct cctggaatt 
      gagtctacg agttatttc gactttatt caaggattg 
      gagtggtta caacccgaa aattctaaa tcgtatttt 
      tccattgac tgcgagtac aagccgcac ttgccccag 
      aatctcaaa gacgataaa gcttcgatt tggctgtac 
      cgaagccta gaaaaccac tctttgtcg cagaaattg 
      tcatggctg ctttccgac aagacacac ttgctgaaa 
      tgctaccaa gaatacgcg tttttgagt caggaaaag 
      tacgcggaa gcggccctc atctgcttg agggcggtg 
      gagagaaac caggctagt ctcatgtcg gaaatcaac 
      ccctgtctc ttccttctc gaagccaac gctaaggaa 
      tttcacaaa atgcaccgc cgttgctcc tcttacccc 
      gacgaccat ttcaaaaaa gactactca gggggtgac 
      aagaagcca aaagggccc ccaactaag acccccgtg 
      ataaggggc aagttgaaa ccgtggcat agtatgccc 
      agtctgcgg tcagactct ttcaaaatc aagagcaaa 
      caaatgttc acacagagt aaaaccact ccgtccacg 
      ccactctat accaaaaaa atttcaact agtgcttta 
      aaaatagat tataagtcg atgcagtat cccaaagta 
      aggaagtcg aatttgaag aagggcttt tcaaaggcg 
      aagaaagag acgaggcat gtgattata aacaattgt 
      gatattatt gagcacacg ccgcctttg agcagctcc 
      cagagtagc ggcggcgct atggggaat gaggatttg 
      atgaatctc tcgactaag tcgttgcct gagacaaga 
      aatttggct cattctccg agacttgtt gacagtttt 
      ttgcctttg ccgggggag aaggactac aggaaagcc 
      cccaaaaag tcttttatt gaggacggg ggcatgagt 
      gtacttccc atggccacg gggtatttt ccaaggccg 
      gtaaagggc cagagtttg ctctccttc ctgacgtcc 
      agtcagttc gctcgagca aacgccgaa ctggatcgc 
      gaaaacgcc catttcagc atctctgaa gccatcatt 
      tcagcgatg gaacaaatc cgatgtcgt cgcgatttc 
      aaactaacc gacgaccaa ttcgacgac agtgatcca 
      gaaatcatg gatttaaag caacgaatt cggttacgg 
      cgacgcgaa aaaatggtc gaaaaacaa aaaaaagtc 
      tggtcggaa tctttatta agcgacgga cgaactgac 
      accgccacc accgtgagc ccctgctcc accacgccc 
      gacaccctc tccgaaagc gccagctcc gacgaagtg 
      gaggacttc gacatagac gaagcttcg aatctttcg 
      gaagaccac gggctttcg gcgtcgatg gcgtcgttg 
      tattcggaa gccgatgtt ttgaaacgg ccacgtggg 
      gcaccagac ggtgcttct gatgtgttt tcagcagaa 
      ggtgtcgcc ttgtcgctc attagcaag ttcaatgaa 
      aagcagttg ccacgagcg tccgatttg gagtggttg 
      gtgtccgaa gaagatgct ccccaagct ctgctaccg 
      ctgccaaag agttggcca gtgagtccg gacgattcc 
      gagacgact tctacgctg ctgaggggg acacaggaa 
      tgggcaccg cccaggccc cagatcatc ttcacgata 
      catccgcca cctacgaga aaaaccctg attgcgaag 
      caaaactac cgatgcgct ggctgtagc atgaaagtg 
      gcgcagcag tacgcctcc aggtaccgc tactgcgac 
      tacttgggt cgctatttc tgcacaggg tgtcacacc 
      aaccaactg gcgttaata ccggggcgt gttatccaa 
      aagtgggat tttacgaga tatccagtt tcctccttc 
      tcgtatcga cttctagaa caaatgtat tccgatccc 
      ttattccga gtcttcgaa ctgaacaaa cacatctgg 
      aagatgtcg aaaaatctc cagttgtgt cgacgattc 
      cgcttgggt ttattctac ttgaaagac tttattttt 
      gcttgtaga tttgccgaa tcaattcgg gagagatta 
      gaacaagaa aaatcgtac cttttgacc gaccctgaa 
      gtttattcc atggaggat ctagtcaat gttagaaac 
      ggtgatatg aaacttagg ctgaaatat ttaatcgag 
      gtgtgctgt agacacact tcggagtgt aaattgtgt 
      gtagcgagg gggtttatc tgtgaaatt tgcaatgcc 
      cctgaagtc atatttccg tggcagatg cgtaaagtc 
      agccgatgt aatcaatgt gggacctgc tatcatatt 
      agttgttgg aaccctagt gataaacca tgtaacaaa 
      tgcttgaga attagcaaa cgtagggaa agtataggg 
      aatagttca cacgacaat ttttaa
    mRNA sequence
    • >TC014378 RA
      gttgatcta acatcatga ttaattcta atccattaa 
      cacgcgtac attttttaa ttgccgttt taaaccagt 
      gcttggaag tagccccct aaaatgtcc gactcggac 
      ccgtacgtg caaaaacac cagcaacta ttacgcgac 
      ttaaaaaac acagttgag gggctgttg gtgtctcaa 
      gtggctaac gtgtggtca atttacggt ggtttgaac 
      cgtctgcac tgctccgtg gagaaaata ttcaaacac 
      gggtgcaaa tctcctgga attgagtct acgagttat 
      ttcgacttt attcaagga ttggagtgg ttacaaccc 
      gaaaattct aaatcgtat ttttccatt gactgcgag 
      tacaagccg cacttgccc cagaatctc aaagacgat 
      aaagcttcg atttggctg taccgaagc ctagaaaac 
      cactctttg tcgcagaaa ttgtcatgg ctgctttcc 
      gacaagaca cacttgctg aaatgctac caagaatac 
      gcgtttttg agtcaggaa aagtacgcg gaagcggcc 
      ctcatctgc ttgagggcg gtggagaga aaccaggct 
      agtctcatg tcggaaatc aacccctgt ctcttcctt 
      ctcgaagcc aacgctaag gaatttcac aaaatgcac 
      cgccgttgc tcctcttac cccgacgac catttcaaa 
      aaagactac tcagggggt gacaagaag ccaaaaggg 
      cccccaact aagaccccc gtgataagg ggcaagttg 
      aaaccgtgg catagtatg cccagtctg cggtcagac 
      tctttcaaa atcaagagc aaacaaatg ttcacacag 
      agtaaaacc actccgtcc acgccactc tataccaaa 
      aaaatttca actagtgct ttaaaaata gattataag 
      tcgatgcag tatcccaaa gtaaggaag tcgaatttg 
      aagaagggc ttttcaaag gcgaagaaa gagacgagg 
      catgtgatt ataaacaat tgtgatatt attgagcac 
      acgccgcct ttgagcagc tcccagagt agcggcggc 
      gctatgggg aatgaggat ttgatgaat ctctcgact 
      aagtcgttg cctgagaca agaaatttg gctcattct 
      ccgagactt gttgacagt tttttgcct ttgccgggg 
      gagaaggac tacaggaaa gcccccaaa aagtctttt 
      attgaggac gggggcatg agtgtactt cccatggcc 
      acggggtat tttccaagg ccggtaaag ggccagagt 
      ttgctctcc ttcctgacg tccagtcag ttcgctcga 
      gcaaacgcc gaactggat cgcgaaaac gcccatttc 
      agcatctct gaagccatc atttcagcg atggaacaa 
      atccgatgt cgtcgcgat ttcaaacta accgacgac 
      caattcgac gacagtgat ccagaaatc atggattta 
      aagcaacga attcggtta cggcgacgc gaaaaaatg 
      gtcgaaaaa caaaaaaaa gtctggtcg gaatcttta 
      ttaagcgac ggacgaact gacaccgcc accaccgtg 
      agcccctgc tccaccacg cccgacacc ctctccgaa 
      agcgccagc tccgacgaa gtggaggac ttcgacata 
      gacgaagct tcgaatctt tcggaagac cacgggctt 
      tcggcgtcg atggcgtcg ttgtattcg gaagccgat 
      gttttgaaa cggccacgt ggggcacca gacggtgct 
      tctgatgtg ttttcagca gaaggtgtc gccttgtcg 
      ctcattagc aagttcaat gaaaagcag ttgccacga 
      gcgtccgat ttggagtgg ttggtgtcc gaagaagat 
      gctccccaa gctctgcta ccgctgcca aagagttgg 
      ccagtgagt ccggacgat tccgagacg acttctacg 
      ctgctgagg gggacacag gaatgggca ccgcccagg 
      ccccagatc atcttcacg atacatccg ccacctacg 
      agaaaaacc ctgattgcg aagcaaaac taccgatgc 
      gctggctgt agcatgaaa gtggcgcag cagtacgcc 
      tccaggtac cgctactgc gactacttg ggtcgctat 
      ttctgcaca gggtgtcac accaaccaa ctggcgtta 
      ataccgggg cgtgttatc caaaagtgg gattttacg 
      agatatcca gtttcctcc ttctcgtat cgacttcta 
      gaacaaatg tattccgat cccttattc cgagtcttc 
      gaactgaac aaacacatc tggaagatg tcgaaaaat 
      ctccagttg tgtcgacga ttccgcttg ggtttattc 
      tacttgaaa gactttatt tttgcttgt agatttgcc 
      gaatcaatt cgggagaga ttagaacaa gaaaaatcg 
      taccttttg accgaccct gaagtttat tccatggag 
      gatctagtc aatgttaga aacggtgat atgaaactt 
      aggctgaaa tatttaatc gaggtgtgc tgtagacac 
      acttcggag tgtaaattg tgtgtagcg agggggttt 
      atctgtgaa atttgcaat gcccctgaa gtcatattt 
      ccgtggcag atgcgtaaa gtcagccga tgtaatcaa 
      tgtgggacc tgctatcat attagttgt tggaaccct 
      agtgataaa ccatgtaac aaatgcttg agaattagc 
      aaacgtagg gaaagtata gggaatagt tcacacgac 
      aatttttaa ttgtgtatt tattcgtta gacaatttt 
      ttataaagt gcaattgtg tatgagatt atttaatat 
      atatttttc gaacaa