Status
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Gene information
General information
TC014376 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9916505..9920793
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014376
    iBeetle screen iB_02280

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially ectopicallypresent - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    thorax partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Many cases where meso- and metathorax seem to be absent, while the prothorax is still present.
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs podomer potentiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen posterior constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014376 RA
      MGGSVSQVF QSGSALLSN GHGHKVSDS TRQKVQTIF 
      DALRANPKI GVQRLEGLL QQIPKNENI LAIHDEAGY 
      NLLQKCVGA NNVELVRWL LARHTAADV NRFPCSLPL 
      HIACLKGHD ECVELLLKH GAKVDVEAR MCWPGAHSS 
      NCEERGKYT SAIQQEENC IERESRDRP SSKLQSAIY 
      YALDGDQVT ILTMLSQRS EDPWNGIFR ARKPLLHAA 
      CERGAWRCT QFLVTERTD EIHILKDEY YPIHYAVLH 
      DSKFLELLI SSGADTTVR TCTQQMTLL HVVLLVAHK 
      SAEDTMSTI RLLLDHGCK ELINTPDSL GNTPLHALI 
      VRYALEEAQ YGYDKWKKW DVLHLVRYL IQSGAGQSI 
      NHAGNSAVA CVLRHIRDW EVCYDLLNM LLHEGGDPN 
      MVGRDGSVP LMVCLVPLI NKDPLHHFT HSMKVCYLN 
      CIRILLKEG ANPNCSYRA NLTPLHVLV FTVSENITL 
      NCDVQKELN FEFIKNLLV LLLIHGLDP NVRVSMRTQ 
      HILQSCMDM IQNVRDSKD IHYVYDLTL TLVQYGANP 
      DLSLNMSEA IKNHPLHLQ SIWKTKNYI LYYYIMLIS 
      RKENLIMDP NMSFSKIIM LFYFIMQHK PLFECLKIL 
      HTQQLSLVP GKMTEPLTC IIRDLYKRP RSLKQICRV 
      KIHNCLGRR PGLYINKLN LPNQLKDYL LNFEP
    CDS sequence
    • >TC014376 RA
      atggggggc tccgtaagc caggtgttc cagtctgga 
      agcgcattg ctgtcgaac ggccacgga cacaaagta 
      tctgactcg accagacaa aaggtacaa accattttc 
      gacgctctc agagccaac ccaaagatt ggagtacaa 
      agactagag gggctgttg caacaaata cccaaaaat 
      gagaatatt ttggctata catgacgag gcgggttat 
      aacctgctg caaaagtgc gtgggggcg aataatgtg 
      gagctagtt cggtggctg ttggctcga cacaccgct 
      gctgatgtc aataggttt ccttgtagt cttccgctt 
      catattgct tgcttaaaa ggtcatgat gaatgtgtg 
      gagttgctg ctgaaacat ggggctaaa gtcgatgtc 
      gaagctcga atgtgctgg ccaggggcc cacagcagt 
      aactgcgag gagagggga aaatacacg tcggccatc 
      cagcaagaa gaaaactgt atcgaacgc gagagccga 
      gaccgtcct tctagcaaa cttcaatca gcaatttac 
      tacgcctta gacggcgac caagtaaca attttgacg 
      atgttatcg caaagaagc gaagaccca tggaacggt 
      atttttcgt gcgaggaaa cctctcctt catgccgct 
      tgcgaacgt ggggcttgg cgctgtact caattctta 
      gttaccgaa agaactgac gaaatccat attctaaag 
      gatgaatat tatccaatc cactacgct gtgctacac 
      gatagcaag tttttagaa cttttaatt agttccggt 
      gctgacacc acggtcagg acgtgtact caacagatg 
      acgttgttg catgttgtt ctcctcgtg gcacacaaa 
      tcggcagaa gatacaatg tcgacaatt cggttattg 
      ttagaccac gggtgcaaa gaattgata aatacgccc 
      gatagttta ggaaataca ccgttacat gcgcttatt 
      gtaagatat gccctggag gaagcccag tacgggtac 
      gataaatgg aaaaaatgg gacgttcta catttggtt 
      aggtattta atccaatcg ggcgccggc cagtcaatc 
      aatcatgcg gggaattcg gccgtggcc tgcgttttg 
      agacatatt cgagattgg gaggtttgt tacgacctg 
      ctcaatatg ttgctgcat gaaggaggc gatcctaat 
      atggtcggg agggacggt tcagtgcct ttaatggtc 
      tgcttagtt cccttaata aataaagat cctcttcat 
      cacttcacg catagcatg aaggtctgt tacttaaac 
      tgcatcagg attttgctg aaagagggc gccaacccc 
      aactgcagc tacagggcc aacttgacg cctctccac 
      gttttagtt ttcaccgtg tcggagaac ataacgttg 
      aactgcgac gttcagaaa gagctcaat ttcgaattc 
      atcaagaat ttgttggtc cttttgttg attcacggt 
      ctcgatccg aacgttaga gttagcatg cggacgcag 
      cacattctg cagtcgtgc atggacatg atacagaac 
      gtgcgcgac tcgaaggat atccactac gtctacgac 
      cttacgctt actttggta caatacggg gcgaatccc 
      gacctgagc ttgaacatg tccgaagca ataaagaac 
      catccgttg cacctccaa tccatttgg aagacgaaa 
      aactacatc ttgtactat tacataatg cttatatcg 
      cgtaaagag aatctcata atggacccg aacatgagc 
      ttttcgaaa ataatcatg ttattttat tttataatg 
      caacacaag ccgctattc gaatgtctg aagattttg 
      cacactcag cagttgagt ttggtaccg gggaaaatg 
      actgagccg ttgacctgt attattaga gatttatac 
      aaaagacca agaagtctg aaacagatc tgccgcgtt 
      aaaatacac aactgtctc gggcgcaga ccgggactc 
      tacattaac aaactgaat ctgcccaat cagctgaaa 
      gattatttg ttgaacttt gaaccgtag
    mRNA sequence
    • >TC014376 RA
      gtgttgtgc gcattataa aagtccttt taaaatgtt 
      ttttcctaa atgtgcttt taaataaca taaataaat 
      aatgtaaat aatgacaac atcattaaa gaccgaccc 
      cagtagcaa gcgtgtcgt cgaaaaagt gccccattt 
      acgtcaatc ccaaactgt caaatttat ctaatcgaa 
      aatgactca aaattgaca gattttagc caaatcgaa 
      ttaaaaatg ggattacgg cagcgttcg gcgtagaca 
      gttttgcct ccagagcag tcatgttga gcagttgtc 
      agtagaccc acctctgag gatgggggg ctccgtaag 
      ccaggtgtt ccagtctgg aagcgcatt gctgtcgaa 
      cggccacgg acacaaagt atctgactc gaccagaca 
      aaaggtaca aaccatttt cgacgctct cagagccaa 
      cccaaagat tggagtaca aagactaga ggggctgtt 
      gcaacaaat acccaaaaa tgagaatat tttggctat 
      acatgacga ggcgggtta taacctgct gcaaaagtg 
      cgtgggggc gaataatgt ggagctagt tcggtggct 
      gttggctcg acacaccgc tgctgatgt caataggtt 
      tccttgtag tcttccgct tcatattgc ttgcttaaa 
      aggtcatga tgaatgtgt ggagttgct gctgaaaca 
      tggggctaa agtcgatgt cgaagctcg aatgtgctg 
      gccaggggc ccacagcag taactgcga ggagagggg 
      aaaatacac gtcggccat ccagcaaga agaaaactg 
      tatcgaacg cgagagccg agaccgtcc ttctagcaa 
      acttcaatc agcaattta ctacgcctt agacggcga 
      ccaagtaac aattttgac gatgttatc gcaaagaag 
      cgaagaccc atggaacgg tatttttcg tgcgaggaa 
      acctctcct tcatgccgc ttgcgaacg tggggcttg 
      gcgctgtac tcaattctt agttaccga aagaactga 
      cgaaatcca tattctaaa ggatgaata ttatccaat 
      ccactacgc tgtgctaca cgatagcaa gtttttaga 
      acttttaat tagttccgg tgctgacac cacggtcag 
      gacgtgtac tcaacagat gacgttgtt gcatgttgt 
      tctcctcgt ggcacacaa atcggcaga agatacaat 
      gtcgacaat tcggttatt gttagacca cgggtgcaa 
      agaattgat aaatacgcc cgatagttt aggaaatac 
      accgttaca tgcgcttat tgtaagata tgccctgga 
      ggaagccca gtacgggta cgataaatg gaaaaaatg 
      ggacgttct acatttggt taggtattt aatccaatc 
      gggcgccgg ccagtcaat caatcatgc ggggaattc 
      ggccgtggc ctgcgtttt gagacatat tcgagattg 
      ggaggtttg ttacgacct gctcaatat gttgctgca 
      tgaaggagg cgatcctaa tatggtcgg gagggacgg 
      ttcagtgcc tttaatggt ctgcttagt tcccttaat 
      aaataaaga tcctcttca tcacttcac gcatagcat 
      gaaggtctg ttacttaaa ctgcatcag gattttgct 
      gaaagaggg cgccaaccc caactgcag ctacagggc 
      caacttgac gcctctcca cgttttagt tttcaccgt 
      gtcggagaa cataacgtt gaactgcga cgttcagaa 
      agagctcaa tttcgaatt catcaagaa tttgttggt 
      ccttttgtt gattcacgg tctcgatcc gaacgttag 
      agttagcat gcggacgca gcacattct gcagtcgtg 
      catggacat gatacagaa cgtgcgcga ctcgaagga 
      tatccacta cgtctacga ccttacgct tactttggt 
      acaatacgg ggcgaatcc cgacctgag cttgaacat 
      gtccgaagc aataaagaa ccatccgtt gcacctcca 
      atccatttg gaagacgaa aaactacat cttgtacta 
      ttacataat gcttatatc gcgtaaaga gaatctcat 
      aatggaccc gaacatgag cttttcgaa aataatcat 
      gttatttta ttttataat gcaacacaa gccgctatt 
      cgaatgtct gaagatttt gcacactca gcagttgag 
      tttggtacc ggggaaaat gactgagcc gttgacctg 
      tattattag agatttata caaaagacc aagaagtct 
      gaaacagat ctgccgcgt taaaataca caactgtct 
      cgggcgcag accgggact ctacattaa caaactgaa 
      tctgcccaa tcagctgaa agattattt gttgaactt 
      tgaaccgta gatcaaggt gactcgaag tcgttcagg 
      atttcttct aatatcggt ttagaatta agtggcgat 
      gtctaaatg tgaccctta gcccagtgt tgaatattg 
      tttctttct cgctcgaaa tcatgaaaa tactcgtcg 
      caagtcaat tatcaaaac gtatcattg tttgacttg 
      tttctggcg ctagctgtg atatgttaa aaaatcctg 
      gacgcgctt cagccgttg ctagttttt aagggagat 
      aatatactt gtgatttct tgttaaaat ttctaaatt 
      ctagtaatc gtaagtgtt cagttgaac ggtacaatg 
      gggtcgact aaaagcaag gatgttgta gaactacga 
      cttatgata tcttaatac gcttttgag ctgtgttcg 
      tgttgaaca gggttttgt gcagattag acagttttt 
      aaatgaacc actcacatt gttgtttat tccgtgcct 
      cttacgttt gaaacacac tgcacacag acgtgtgcg 
      aacggtttt tggttccgt caccggttt ttctttgac 
      aataatatt tagcttggt ttgttgtag gaatctggc 
      aaagtccta tgctataca aaatatcag ctctgtaca 
      ttttaatat atatgccaa atattatta agagctaag 
      tataatata taaattgga tagtatatt ttatgttaa 
      gatttaaga ttatgtgat tttattgtt gaggcatat 
      tggtatcat atttggata aaaagtatt taataaaaa 
      aatgtctat att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY