Status
Please wait ...
Gene information
General information
TC014350 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9514911..9517786
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014350
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02278

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    egg chamber number decreased - Number of animals showing the phenotype: 1

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Comment: one animal developed adult structures still inside the larval cuticle, pupal cuticular structures (gin traps) are visible as well

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014350 RA
      MAATTYLPA SSAVSADLD VGMNIVGGY HASASPRSA 
      ADANEMKYL PQHHHHHNH HMASSPSPG GHAAVSLSA 
      ANPWVSLQP GADPWAASM AGMHHHHHH PHQDVKPLQ 
      QDMMHHRQQ QQQSMGSPH WHAPVSSAH YNPAGAGSP 
      LQQYHAAMN GMLAHHQGA PQLHHPLHR DMQSPHHPQ 
      HSDRDVSGG EDETPTSDD LEAFAKQFK QRRIKLGFT 
      QADVGLALG TLYGNVFSQ TTICRFEAL QLSFKNMCK 
      LKPLLQKWL EEADSTTGS PTSIDKIAA QGRKRKKRT 
      SIEVSVKGA LEQHFHKQP KPSAQEISS LADSLQLEK 
      EVVRVWFCN RRQKEKRMT PPNTLGNEM MEGMPPGSH 
      MHPGYGHHP DMHGSPMGQ HSHSHSPPM LSPQGMGHQ 
      LTAH
    CDS sequence
    • >TC014350 RA
      atggcggcc acgacgtac cttccggcg agcagtgcg 
      gtaagtgcg gacctggac gtagggatg aacatcgtc 
      ggcggctac cacgccagc gcttcgccg cggtcggcg 
      gcggacgcc aacgagatg aagtacctg ccgcagcac 
      caccaccat cacaaccac cacatggcg agctcgccg 
      tcgcccggc gggcacgcg gccgtctcg ctctccgcc 
      gccaacccc tgggtgagc ctgcagccg ggcgccgac 
      ccctgggcc gcctccatg gccggcatg caccaccac 
      catcaccac ccgcaccag gacgtcaaa ccactgcag 
      caggacatg atgcaccac cgccagcag cagcaacag 
      agcatgggc tcgccgcac tggcacgcg cccgtctcc 
      tcggctcat tacaacccg gcgggcgcg ggctcgcct 
      cttcaacaa taccatgcc gctatgaac gggatgctg 
      gcgcaccac caaggcgct ccccagctt caccaccct 
      cttcatcgg gacatgcaa agcccgcac cacccccaa 
      cactcagac cgcgacgtg agcggcggc gaggatgaa 
      acccccacg agcgatgac ctggaggcc ttcgccaaa 
      cagttcaaa cagcgtcgc attaaactc ggatttacg 
      caagcggac gtcggactc gccctcgga accctctac 
      ggaaacgtc ttctcccag accacgata tgccgattc 
      gaggcgtta caattaagt tttaaaaat atgtgtaaa 
      ttgaaaccg ctgttacaa aagtggctc gaggaggcc 
      gattcgacg accggctct ccgacgtcc atcgataaa 
      atcgcggcg cagggccgc aaacggaaa aagcgcacc 
      agcatcgaa gtctccgtg aaaggcgcc ctcgagcag 
      cacttccac aaacagcca aaaccgtca gctcaagaa 
      atatcgagt ttggccgat agtctccaa ttggaaaag 
      gaagtagtg agagtgtgg ttctgcaat cggcggcag 
      aaagaaaaa aggatgacg cctccaaac acactcgga 
      aacgagatg atggaggga atgccgccg ggaagtcac 
      atgcacccc ggttacggg caccacccg gacatgcac 
      gggtcgccc atgggccaa cattcgcat tcccacagt 
      cctcccatg ctatctccc caaggcatg ggccatcaa 
      ctgacggcg cactag
    mRNA sequence
    • >TC014350 RA
      gccgtgcgg tatgcataa cgcatgaga gctgaccat 
      tagagtttt ttggtttga tagcataaa tgacagtcc 
      gtggttggt ggcggcgat aacggccgt gatctagtg 
      agtgtcgca aacggtggc aaagtgtac gaacacgaa 
      gacagtgtg gactcggcg gttgggcgg cccttgcag 
      ggcatggcg gccacgacg taccttccg gcgagcagt 
      gcggtaagt gcggacctg gacgtaggg atgaacatc 
      gtcggcggc taccacgcc agcgcttcg ccgcggtcg 
      gcggcggac gccaacgag atgaagtac ctgccgcag 
      caccaccac catcacaac caccacatg gcgagctcg 
      ccgtcgccc ggcgggcac gcggccgtc tcgctctcc 
      gccgccaac ccctgggtg agcctgcag ccgggcgcc 
      gacccctgg gccgcctcc atggccggc atgcaccac 
      caccatcac cacccgcac caggacgtc aaaccactg 
      cagcaggac atgatgcac caccgccag cagcagcaa 
      cagagcatg ggctcgccg cactggcac gcgcccgtc 
      tcctcggct cattacaac ccggcgggc gcgggctcg 
      cctcttcaa caataccat gccgctatg aacgggatg 
      ctggcgcac caccaaggc gctccccag cttcaccac 
      cctcttcat cgggacatg caaagcccg caccacccc 
      caacactca gaccgcgac gtgagcggc ggcgaggat 
      gaaaccccc acgagcgat gacctggag gccttcgcc 
      aaacagttc aaacagcgt cgcattaaa ctcggattt 
      acgcaagcg gacgtcgga ctcgccctc ggaaccctc 
      tacggaaac gtcttctcc cagaccacg atatgccga 
      ttcgaggcg ttacaatta agttttaaa aatatgtgt 
      aaattgaaa ccgctgtta caaaagtgg ctcgaggag 
      gccgattcg acgaccggc tctccgacg tccatcgat 
      aaaatcgcg gcgcagggc cgcaaacgg aaaaagcgc 
      accagcatc gaagtctcc gtgaaaggc gccctcgag 
      cagcacttc cacaaacag ccaaaaccg tcagctcaa 
      gaaatatcg agtttggcc gatagtctc caattggaa 
      aaggaagta gtgagagtg tggttctgc aatcggcgg 
      cagaaagaa aaaaggatg acgcctcca aacacactc 
      ggaaacgag atgatggag ggaatgccg ccgggaagt 
      cacatgcac cccggttac gggcaccac ccggacatg 
      cacgggtcg cccatgggc caacattcg cattcccac 
      agtcctccc atgctatct ccccaaggc atgggccat 
      caactgacg gcgcactag catccagcc cactgggcc 
      tccctattg caccacagc gataacggc tagtgatcg 
      tattacatt aatagaacg ccgccccca aaatacagt 
      gtgaaaata atacaaatt gctgataga aatgatgaa 
      aatgccgac ggcgcgtga ctgactgtt ccgtttgtt 
      atcaacgct acctcaaag gattcgtgt taaactcct 
      gccgtcgcg gaggtccta gtggactga ctcaaaaac 
      tagtttcta ctgtcttat tattaattt ttctgttgt 
      taatacgcc gtgcagtgc gagtgagta ccttgtcat 
      cgtcataaa ctggacttg tttgtatat tacctgtaa 
      ataagagag tgataacgc gtctccaga cgtgagtgt 
      aatataata ataggcgaa tgctgctaa ttttacgtt 
      tttgtaaat aagaagaat cgactactg ttgggagca 
      gtagcaagt cgtgattta tacttaaaa atatacacc 
      gggacggtt gacgaataa aacaattta caatgtaag 
      tttataatg tatagaatg tcgtatata tttaaaaaa 
      tataaagtt gttcttatg ttgtttatt aaagttttt 
      tggttagtg tcgtgcgat ccagttgaa ctctctttg 
      acttttatt tttggataa taacgaaat gagaaattc 
      attcaaacg aatcggaac cgtacctac tcttgtctc 
      tttggtcat agattcatg tgtacatat ttattatta 
      taattataa ttaattata tatcgttgt acggtgtga 
      ctaaattaa aacgaaaaa tgtttttaa aatagccct 
      ctctgctgt attatttca atcgtaccc ccgtttttt 
      cgctttatc attcatcgt tcatttcta gtcagtgtt 
      taattcaag aaaaaaaag ggaaaatta aaagtactt 
      tgtgttgcg atgcgacgg atcgtttta tacctctcg 
      gggtgagtc ttgttttcg gaaaatcca tttaaaacc 
      ctcgtaaga ggtatgaat tgacatttg accggaggt 
      acggcgatt atctttgaa tcccatgtt gagcacttt 
      atcaaatat ttctcagac acatgtgac aaacacggc 
      ccataaata tgctaaatt atatgacag tggtgtgtg 
      ttaatcaac gaaatttct gctatgtag aaacacgtt 
      cagttatac ggtctcgaa acctgttta agcagttat 
      ttgggaaaa aataaccat ttaaaacga ccattatta 
      gttgagttg atagctaat ggacataaa tttcatcaa 
      aaaaccata gaataacgc aatccccgt agagattcg 
      tttccaaat tggtattcc gtacgtctc gatagttcc 
      tccagaaat tttcgtttc gaaaacaac tatgtataa 
      aaactgttt ttgtaaaaa acaatgttt actgtacta 
      tagagagag aggatattt gtgtatatt gtattttta 
      tcaatgtcc aaaaataaa aatcaataa aatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY