Status
Please wait ...
Gene information
General information
TC014338 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9376621..9377590
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014338
    iBeetle screen iB_02276

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellarium shortened - Number of animals showing the phenotype: 4

    egg chamber potentially sometimesfused - Number of animals showing the phenotype: 4

    egg chamber potentially ectopicallypresent - Number of animals showing the phenotype: 4

    tropharium shape irregular - Number of animals showing the phenotype: 4
    tropharium shortened - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014338 RA
      MTLVEFFGC TFLAFGPPF AMFVFTIAH 
      DPVRIIILI AAAFFWLVS LLLSSLWWY 
      IVVPLRQDL AFGVVFSVF FQETFRYII 
      YKILRKAEN GLKKITDDS TQLIENKHI 
      LAYVSGLGF GIISGAFSL VNILADAVG 
      PGTMGLKGG NEMFFLTSA ATGLCFTLL 
      HTFWGVIFF NALDNSNKC YVGVVVVSH 
      LLSSCITLL NFKVGPAGT LIPLYFNLV 
      LMGLMAYRV AGGSYASFK SCITFK
    CDS sequence
    • >TC014338 RA
      atgactttg gttgagttc ttcgggtgc acctttttg 
      gcctttggc cctccgttt gcgatgttc gtgttcaca 
      atcgcccat gaccctgtg cgaatcata atcctgata 
      gcggcagcg ttcttctgg ctggtctcc cttctgttg 
      tcttcgttg tggtggtac attgtggtc ccgctgagg 
      caagacttg gcttttgga gtggtcttc tcagtgttc 
      ttccaggaa accttccgc tacatcatt tacaaaatc 
      ctgagaaaa gcggaaaat ggccttaag aaaatcacc 
      gacgacagc actcagctg attgaaaac aagcatatt 
      ctggcctat gtcagtggg ctgggtttt gggattatc 
      agcggggcc ttttcgttg gtcaacatc cttgctgac 
      gcagtgggt cctgggacc atgggactg aaaggcggg 
      aatgagatg tttttcctc acaagtgca gcaactgga 
      ctttgtttt acattgttg catactttc tggggtgtg 
      atttttttc aacgctcta gataatagt aataagtgt 
      tacgtggga gttgttgtg gtttcgcac cttttgtcc 
      tcttgtatt acgttgctt aattttaaa gtcggacca 
      gcaggcact ttaatccca ctgtatttt aatctagtc 
      ctcatgggg ttaatggca tacagggtc gcaggtggc 
      tcatatgcc tcatttaaa agctgtata acttttaag 
      taa
    mRNA sequence
    • >TC014338 RA
      catttcgac tgtcatgta gatctttga agctttttc 
      gaaaataaa ggaaaatga ctttggttg agttcttcg 
      ggtgcacct ttttggcct ttggccctc cgtttgcga 
      tgttcgtgt tcacaatcg cccatgacc ctgtgcgaa 
      tcataatcc tgatagcgg cagcgttct tctggctgg 
      tctcccttc tgttgtctt cgttgtggt ggtacattg 
      tggtcccgc tgaggcaag acttggctt ttggagtgg 
      tcttctcag tgttcttcc aggaaacct tccgctaca 
      tcatttaca aaatcctga gaaaagcgg aaaatggcc 
      ttaagaaaa tcaccgacg acagcactc agctgattg 
      aaaacaagc atattctgg cctatgtca gtgggctgg 
      gttttggga ttatcagcg gggcctttt cgttggtca 
      acatccttg ctgacgcag tgggtcctg ggaccatgg 
      gactgaaag gcgggaatg agatgtttt tcctcacaa 
      gtgcagcaa ctggacttt gttttacat tgttgcata 
      ctttctggg gtgtgattt ttttcaacg ctctagata 
      atagtaata agtgttacg tgggagttg ttgtggttt 
      cgcaccttt tgtcctctt gtattacgt tgcttaatt 
      ttaaagtcg gaccagcag gcactttaa tcccactgt 
      attttaatc tagtcctca tggggttaa tggcataca 
      gggtcgcag gtggctcat atgcctcat ttaaaagct 
      gtataactt ttaagtaat ttattagag aaataaaac 
      ttttcataa tatataatt atttcggta ttatctgac 
      ccacaacaa ccattcttt tgattacac tttatgttt 
      tatccaatg taaatctct tgtttgact gaaaagtta 
      tgtttacta catttttat ttatttaaa tgtagagat 
      ttttgtaac taattaata aaaccttca aagaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY