Status
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Gene information
General information
TC014335 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9279452..9286486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014335
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 4

    antenna orientation irregular - Number of animals showing the phenotype: 3
    prothoracic leg shortened - Number of animals showing the phenotype: 4

    wings shortened - Number of animals showing the phenotype: 5

    wings partially potentiallynecrotic - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014335 RA
      MKKPEEDKN FSIKSENSC KNKKRRTGV HKMETATES 
      LIFENHRFL STLSLGKRK ISEDLPLGT HGTFRQKSS 
      TFEHTLSFS GCARAARQS IGNIGNILS RKMAASTSQ 
      PSVPWPNSK DDYELGKVI GVGATAVVH GAFCKPRNE 
      KCAIKRINL EKWNTSMDE LLKEIQAMS SCNHENVVT 
      YYTSFVVRE ELWLVLRLL EGGSLLDII KHKMRVANC 
      KHGVFDEST IATVLREVL KGLEYFHSN GQIHRDIKA 
      GNILLGEDG TVQIADFGV SAWLATGRD LSRQKVRHT 
      FVGTPCWMA PEVMEQDHG YDFKADIWS FGITAIEMA 
      TGTAPYHKY PPMKVLMLT LQNDPPNLD TGAEEKDQY 
      KAYGKTFRK MITDCLQKD ASKRPTASE LLKHPFFKK 
      AKDKKYLQQ TLVAIGPSL ETRVQKASK RQPGASGRL 
      HRKETGEWV WSSEDEGED AASSESDTD SKPMNTLAS 
      AGSSGSDHE SSEGDPPPV NLVLRMRNA KRELNDIRF 
      EFAVGKDSA EGIASELVG AGLVDGKDI EVITQNLQK 
      LIDSRDMIK VVTFPLNSG LTNNETPDD KALIGFAQI 
      SITD
    CDS sequence
    • >TC014335 RA
      atgaaaaaa cccgaagaa gacaaaaac ttcagcatc 
      aaaagcgaa aacagttgc aagaataaa aaaagaaga 
      accggagtg cataaaatg gaaacagca actgaaagt 
      ctaattttt gaaaatcat cgctttctc agcacgctt 
      tcgctagga aaaagaaaa atatcggaa gatttgccc 
      ctggggacc catggcacc tttcgtcaa aaatcatca 
      acttttgaa cacacgtta tcatttagc ggctgcgcc 
      cgagctgct cgccagtcg atcggtaat ataggaaac 
      atactgtcg agaaagatg gcggcgtca acgtctcaa 
      ccgtcagtc ccttggcca aattccaag gatgattac 
      gaattaggc aaagtaatc ggtgttgga gcaacagca 
      gtggtccat ggagcgttt tgtaagccc agaaatgag 
      aaatgtgcc atcaaaagg atcaatttg gaaaaatgg 
      aacacctcc atggacgaa ttactcaaa gaaatccaa 
      gccatgtcc agctgcaac cacgaaaac gtggtcact 
      tactacacc agttttgtc gtgagggag gaattatgg 
      cttgtcttg agattactc gaaggagga agtttgttg 
      gacataatt aagcacaaa atgcgcgtg gcaaactgc 
      aaacatgga gtattcgac gagtcaaca attgccact 
      gttttacgg gaagtttta aaaggtcta gagtacttc 
      cacagtaac ggtcagatt cacagagat attaaagct 
      ggaaatatc ttgctcggt gaagacggc actgttcaa 
      attgccgat tttggagtc tctgcctgg ttggcaact 
      ggcagagac ctctcccgg cagaaagtc cggcacaca 
      tttgtcgga actccttgt tggatggct ccagaggtc 
      atggaacag gaccatggt tatgacttt aaggccgac 
      atttggtcg tttggaatc accgccatt gaaatggcg 
      acaggcaca gccccatat cacaaatac cccccaatg 
      aaggtactt atgctcact ctccagaac gatccgccg 
      aatttagac acaggagcc gaggaaaag gatcagtac 
      aaagcttac ggtaaaacg ttcagaaaa atgatcaca 
      gactgtctg caaaaggat gcatccaag cggcccacg 
      gcttcagaa ttgttaaag cacccgttt ttcaaaaag 
      gcgaaagat aagaaatac ttacagcag acactggtg 
      gctataggg ccgagtttg gagacgagg gtgcaaaag 
      gcgtcgaaa aggcaacct ggcgcctct ggaaggttg 
      cacaggaag gagacgggt gaatgggtg tggtcgagt 
      gaggatgaa ggagaagat gcggcctct agtgagagc 
      gacactgac tcgaaaccc atgaatacg ttagcgtca 
      gctgggtcg tcagggtca gaccacgaa agtagtgaa 
      ggtgaccca ccgccggtg aacctagtg ctacgaatg 
      agaaatgcc aagcgcgaa ttaaacgac atccggttt 
      gaattcgcc gttgggaaa gattcggcc gaaggcatt 
      gccagtgag ttggtgggt gctgggctt gtagatggt 
      aaagatatt gaggtcatc actcaaaat cttcagaag 
      cttattgat tcgagagac atgataaaa gttgtaact 
      tttccattg aattcagga ctgactaat aatgaaact 
      ccggatgat aaggcactg ataggattt gcacaaata 
      tcaatcact gactga
    mRNA sequence
    • >TC014335 RA
      tttatcggg aactgaatt ttgggaatt tcgataaaa 
      aagaaagtt tgttcagca gtaaagttt atttgctta 
      tacaaataa aaatgtgtt aaaaataat ttatgaaaa 
      atccagtcc tttattgta catttagat gtttactga 
      tgacgacac ttgcatccc tgttccact ggaatggtg 
      cgagtatgt ttccaacag ttatgtttt tttgtaaaa 
      ataggaact tgacgatag gctgtttca cacgttgga 
      ttatacacc actgtacaa aaacccatc atgactgac 
      tcctggtgt gatccagtg gcgggttta caagttata 
      ttgtggtac ctctttgga tatatctcc tcatcttct 
      gacattttt ggaacatcg tgtactgtc catttttct 
      acgcatcct aacttattt ctcctctat aaattacaa 
      aacacttaa aatatttcg aaataagca gcaactcga 
      tgcttttac tgtgtgtcc agttggtat tccacgagt 
      ttttagaga atcatcagt atcctctaa aagctgtat 
      atcatgttc gatttgatc ccaaacatc ataatcgtc 
      ataattatt attatcata attatttat gttatttaa 
      ataatttat atctgccac ctcggcaga atcagtgtc 
      aaaattgta atcatttat cggccactg ctctgctga 
      attttttga taaaaatga gaagacaat caatacctg 
      aaaataaga aacgtagtg taaaatgaa acaaaacat 
      ctgactcag tttcggcac agcctgtat ctgagttgt 
      tgttaagta tgtcaccta cgtataaaa cctactaat 
      tgattttaa tggataaat tagatcatt ttcttccat 
      ggcccctaa ggtcttgta tagatacat taagtttga 
      ttaaatgga gagaattac gaattcaag ttcgcattg 
      tagtgttct aaagccagc agccttgca ggcgaaaaa 
      gttttaaac atgaagtga tgcagttac aaatgtgtg 
      aaacttggc ggacatttt cctgtattg ttagatcaa 
      aactgtgat tttaataat aaatgacca tgaaaaaac 
      ccgaagaag acaaaaact tcagcatca aaagcgaaa 
      acagttgca agaataaaa aaagaagaa ccggagtgc 
      ataaaatgg aaacagcaa ctgaaagtc taatttttg 
      aaaatcatc gctttctca gcacgcttt cgctaggaa 
      aaagaaaaa tatcggaag atttgcccc tggggaccc 
      atggcacct ttcgtcaaa aatcatcaa cttttgaac 
      acacgttat catttagcg gctgcgccc gagctgctc 
      gccagtcga tcggtaata taggaaaca tactgtcga 
      gaaagatgg cggcgtcaa cgtctcaac cgtcagtcc 
      cttggccaa attccaagg atgattacg aattaggca 
      aagtaatcg gtgttggag caacagcag tggtccatg 
      gagcgtttt gtaagccca gaaatgaga aatgtgcca 
      tcaaaagga tcaatttgg aaaaatgga acacctcca 
      tggacgaat tactcaaag aaatccaag ccatgtcca 
      gctgcaacc acgaaaacg tggtcactt actacacca 
      gttttgtcg tgagggagg aattatggc ttgtcttga 
      gattactcg aaggaggaa gtttgttgg acataatta 
      agcacaaaa tgcgcgtgg caaactgca aacatggag 
      tattcgacg agtcaacaa ttgccactg ttttacggg 
      aagttttaa aaggtctag agtacttcc acagtaacg 
      gtcagattc acagagata ttaaagctg gaaatatct 
      tgctcggtg aagacggca ctgttcaaa ttgccgatt 
      ttggagtct ctgcctggt tggcaactg gcagagacc 
      tctcccggc agaaagtcc ggcacacat ttgtcggaa 
      ctccttgtt ggatggctc cagaggtca tggaacagg 
      accatggtt atgacttta aggccgaca tttggtcgt 
      ttggaatca ccgccattg aaatggcga caggcacag 
      ccccatatc acaaatacc ccccaatga aggtactta 
      tgctcactc tccagaacg atccgccga atttagaca 
      caggagccg aggaaaagg atcagtaca aagcttacg 
      gtaaaacgt tcagaaaaa tgatcacag actgtctgc 
      aaaaggatg catccaagc ggcccacgg cttcagaat 
      tgttaaagc acccgtttt tcaaaaagg cgaaagata 
      agaaatact tacagcaga cactggtgg ctatagggc 
      cgagtttgg agacgaggg tgcaaaagg cgtcgaaaa 
      ggcaacctg gcgcctctg gaaggttgc acaggaagg 
      agacgggtg aatgggtgt ggtcgagtg aggatgaag 
      gagaagatg cggcctcta gtgagagcg acactgact 
      cgaaaccca tgaatacgt tagcgtcag ctgggtcgt 
      cagggtcag accacgaaa gtagtgaag gtgacccac 
      cgccggtga acctagtgc tacgaatga gaaatgcca 
      agcgcgaat taaacgaca tccggtttg aattcgccg 
      ttgggaaag attcggccg aaggcattg ccagtgagt 
      tggtgggtg ctgggcttg tagatggta aagatattg 
      aggtcatca ctcaaaatc ttcagaagc ttattgatt 
      cgagagaca tgataaaag ttgtaactt ttccattga 
      attcaggac tgactaata atgaaactc cggatgata 
      aggcactga taggatttg cacaaatat caatcactg 
      actgaaaca gactggacc gtaatcgct aatatatct 
      cctggaatc tactgatca aattgtata tagcctagg 
      taggtttta taaattaac tctaatttt atgcactac 
      caaaaacgt ttccgattt gttcacgtc atgatattt 
      ttattaatt tatttcgcg aaattatgt tgtgatcaa 
      tagatttca agtatttgt acttaatag tttttacat 
      agttatttg cagctgtca atataattg ttacaataa 
      ggtctatta tagtttatt ccaactgtc atgttttta 
      aagattttt attgaactg ttagatttt aaggaaacc 
      atattgcct tttttagaa aagctagaa atgtaacag 
      attggtttt gcttcatac taaaaatat ttaattagt 
      tttaatacc ttgaatatg gcctatgat acggcaagc 
      caatatgtt gatgagtga tgaatataa taatttgta 
      tgtgggatt ggtaacgtt gtaaatata ttatttatt 
      tatttcaat aatgtagat gcaatatta agaaatgat 
      caaaaacag tggagtact tattgtctt tttcgtaac 
      ttaagcaaa agacttgta tttcctgcc tgatacttt 
      cttgttcat ccttctcat ctccaaatt gttgaacgc 
      aacgtgcgt actacacaa ctccataac tgagtaatt 
      ttatgatat ttttatagt aaaaaatta caataatta 
      tgatttccc ccatgttat ttgagtatc aaaattttt 
      gtaacccat aatctgtaa ctctcaatt ttaatagaa 
      tatcaaaaa ctccaacag tgaagtcta atatgatcc 
      tcaaacaaa aataaatat tcattgtta tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY