Status
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Gene information
General information
TC014335 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9279452..9286486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014335
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 4

    antenna orientation irregular - Number of animals showing the phenotype: 3
    prothoracic leg shortened - Number of animals showing the phenotype: 4

    wings shortened - Number of animals showing the phenotype: 5

    wings partially potentiallynecrotic - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014335 RA
      MKKPEEDKN FSIKSENSC KNKKRRTGV HKMETATES 
LIFENHRFL STLSLGKRK ISEDLPLGT HGTFRQKSS 
TFEHTLSFS GCARAARQS IGNIGNILS RKMAASTSQ 
PSVPWPNSK DDYELGKVI GVGATAVVH GAFCKPRNE 
KCAIKRINL EKWNTSMDE LLKEIQAMS SCNHENVVT 
YYTSFVVRE ELWLVLRLL EGGSLLDII KHKMRVANC 
KHGVFDEST IATVLREVL KGLEYFHSN GQIHRDIKA 
GNILLGEDG TVQIADFGV SAWLATGRD LSRQKVRHT 
FVGTPCWMA PEVMEQDHG YDFKADIWS FGITAIEMA 
TGTAPYHKY PPMKVLMLT LQNDPPNLD TGAEEKDQY 
KAYGKTFRK MITDCLQKD ASKRPTASE LLKHPFFKK 
AKDKKYLQQ TLVAIGPSL ETRVQKASK RQPGASGRL 
HRKETGEWV WSSEDEGED AASSESDTD SKPMNTLAS 
AGSSGSDHE SSEGDPPPV NLVLRMRNA KRELNDIRF 
EFAVGKDSA EGIASELVG AGLVDGKDI EVITQNLQK 
LIDSRDMIK VVTFPLNSG LTNNETPDD KALIGFAQI 
SITD
    CDS sequence
    • >TC014335 RA
      atgaaaaaa cccgaagaa gacaaaaac ttcagcatc 
aaaagcgaa aacagttgc aagaataaa aaaagaaga 
accggagtg cataaaatg gaaacagca actgaaagt 
ctaattttt gaaaatcat cgctttctc agcacgctt 
tcgctagga aaaagaaaa atatcggaa gatttgccc 
ctggggacc catggcacc tttcgtcaa aaatcatca 
acttttgaa cacacgtta tcatttagc ggctgcgcc 
cgagctgct cgccagtcg atcggtaat ataggaaac 
atactgtcg agaaagatg gcggcgtca acgtctcaa 
ccgtcagtc ccttggcca aattccaag gatgattac 
gaattaggc aaagtaatc ggtgttgga gcaacagca 
gtggtccat ggagcgttt tgtaagccc agaaatgag 
aaatgtgcc atcaaaagg atcaatttg gaaaaatgg 
aacacctcc atggacgaa ttactcaaa gaaatccaa 
gccatgtcc agctgcaac cacgaaaac gtggtcact 
tactacacc agttttgtc gtgagggag gaattatgg 
cttgtcttg agattactc gaaggagga agtttgttg 
gacataatt aagcacaaa atgcgcgtg gcaaactgc 
aaacatgga gtattcgac gagtcaaca attgccact 
gttttacgg gaagtttta aaaggtcta gagtacttc 
cacagtaac ggtcagatt cacagagat attaaagct 
ggaaatatc ttgctcggt gaagacggc actgttcaa 
attgccgat tttggagtc tctgcctgg ttggcaact 
ggcagagac ctctcccgg cagaaagtc cggcacaca 
tttgtcgga actccttgt tggatggct ccagaggtc 
atggaacag gaccatggt tatgacttt aaggccgac 
atttggtcg tttggaatc accgccatt gaaatggcg 
acaggcaca gccccatat cacaaatac cccccaatg 
aaggtactt atgctcact ctccagaac gatccgccg 
aatttagac acaggagcc gaggaaaag gatcagtac 
aaagcttac ggtaaaacg ttcagaaaa atgatcaca 
gactgtctg caaaaggat gcatccaag cggcccacg 
gcttcagaa ttgttaaag cacccgttt ttcaaaaag 
gcgaaagat aagaaatac ttacagcag acactggtg 
gctataggg ccgagtttg gagacgagg gtgcaaaag 
gcgtcgaaa aggcaacct ggcgcctct ggaaggttg 
cacaggaag gagacgggt gaatgggtg tggtcgagt 
gaggatgaa ggagaagat gcggcctct agtgagagc 
gacactgac tcgaaaccc atgaatacg ttagcgtca 
gctgggtcg tcagggtca gaccacgaa agtagtgaa 
ggtgaccca ccgccggtg aacctagtg ctacgaatg 
agaaatgcc aagcgcgaa ttaaacgac atccggttt 
gaattcgcc gttgggaaa gattcggcc gaaggcatt 
gccagtgag ttggtgggt gctgggctt gtagatggt 
aaagatatt gaggtcatc actcaaaat cttcagaag 
cttattgat tcgagagac atgataaaa gttgtaact 
tttccattg aattcagga ctgactaat aatgaaact 
ccggatgat aaggcactg ataggattt gcacaaata 
tcaatcact gactga
    mRNA sequence
    • >TC014335 RA
      tttatcggg aactgaatt ttgggaatt tcgataaaa 
aagaaagtt tgttcagca gtaaagttt atttgctta 
tacaaataa aaatgtgtt aaaaataat ttatgaaaa 
atccagtcc tttattgta catttagat gtttactga 
tgacgacac ttgcatccc tgttccact ggaatggtg 
cgagtatgt ttccaacag ttatgtttt tttgtaaaa 
ataggaact tgacgatag gctgtttca cacgttgga 
ttatacacc actgtacaa aaacccatc atgactgac 
tcctggtgt gatccagtg gcgggttta caagttata 
ttgtggtac ctctttgga tatatctcc tcatcttct 
gacattttt ggaacatcg tgtactgtc catttttct 
acgcatcct aacttattt ctcctctat aaattacaa 
aacacttaa aatatttcg aaataagca gcaactcga 
tgcttttac tgtgtgtcc agttggtat tccacgagt 
ttttagaga atcatcagt atcctctaa aagctgtat 
atcatgttc gatttgatc ccaaacatc ataatcgtc 
ataattatt attatcata attatttat gttatttaa 
ataatttat atctgccac ctcggcaga atcagtgtc 
aaaattgta atcatttat cggccactg ctctgctga 
attttttga taaaaatga gaagacaat caatacctg 
aaaataaga aacgtagtg taaaatgaa acaaaacat 
ctgactcag tttcggcac agcctgtat ctgagttgt 
tgttaagta tgtcaccta cgtataaaa cctactaat 
tgattttaa tggataaat tagatcatt ttcttccat 
ggcccctaa ggtcttgta tagatacat taagtttga 
ttaaatgga gagaattac gaattcaag ttcgcattg 
tagtgttct aaagccagc agccttgca ggcgaaaaa 
gttttaaac atgaagtga tgcagttac aaatgtgtg 
aaacttggc ggacatttt cctgtattg ttagatcaa 
aactgtgat tttaataat aaatgacca tgaaaaaac 
ccgaagaag acaaaaact tcagcatca aaagcgaaa 
acagttgca agaataaaa aaagaagaa ccggagtgc 
ataaaatgg aaacagcaa ctgaaagtc taatttttg 
aaaatcatc gctttctca gcacgcttt cgctaggaa 
aaagaaaaa tatcggaag atttgcccc tggggaccc 
atggcacct ttcgtcaaa aatcatcaa cttttgaac 
acacgttat catttagcg gctgcgccc gagctgctc 
gccagtcga tcggtaata taggaaaca tactgtcga 
gaaagatgg cggcgtcaa cgtctcaac cgtcagtcc 
cttggccaa attccaagg atgattacg aattaggca 
aagtaatcg gtgttggag caacagcag tggtccatg 
gagcgtttt gtaagccca gaaatgaga aatgtgcca 
tcaaaagga tcaatttgg aaaaatgga acacctcca 
tggacgaat tactcaaag aaatccaag ccatgtcca 
gctgcaacc acgaaaacg tggtcactt actacacca 
gttttgtcg tgagggagg aattatggc ttgtcttga 
gattactcg aaggaggaa gtttgttgg acataatta 
agcacaaaa tgcgcgtgg caaactgca aacatggag 
tattcgacg agtcaacaa ttgccactg ttttacggg 
aagttttaa aaggtctag agtacttcc acagtaacg 
gtcagattc acagagata ttaaagctg gaaatatct 
tgctcggtg aagacggca ctgttcaaa ttgccgatt 
ttggagtct ctgcctggt tggcaactg gcagagacc 
tctcccggc agaaagtcc ggcacacat ttgtcggaa 
ctccttgtt ggatggctc cagaggtca tggaacagg 
accatggtt atgacttta aggccgaca tttggtcgt 
ttggaatca ccgccattg aaatggcga caggcacag 
ccccatatc acaaatacc ccccaatga aggtactta 
tgctcactc tccagaacg atccgccga atttagaca 
caggagccg aggaaaagg atcagtaca aagcttacg 
gtaaaacgt tcagaaaaa tgatcacag actgtctgc 
aaaaggatg catccaagc ggcccacgg cttcagaat 
tgttaaagc acccgtttt tcaaaaagg cgaaagata 
agaaatact tacagcaga cactggtgg ctatagggc 
cgagtttgg agacgaggg tgcaaaagg cgtcgaaaa 
ggcaacctg gcgcctctg gaaggttgc acaggaagg 
agacgggtg aatgggtgt ggtcgagtg aggatgaag 
gagaagatg cggcctcta gtgagagcg acactgact 
cgaaaccca tgaatacgt tagcgtcag ctgggtcgt 
cagggtcag accacgaaa gtagtgaag gtgacccac 
cgccggtga acctagtgc tacgaatga gaaatgcca 
agcgcgaat taaacgaca tccggtttg aattcgccg 
ttgggaaag attcggccg aaggcattg ccagtgagt 
tggtgggtg ctgggcttg tagatggta aagatattg 
aggtcatca ctcaaaatc ttcagaagc ttattgatt 
cgagagaca tgataaaag ttgtaactt ttccattga 
attcaggac tgactaata atgaaactc cggatgata 
aggcactga taggatttg cacaaatat caatcactg 
actgaaaca gactggacc gtaatcgct aatatatct 
cctggaatc tactgatca aattgtata tagcctagg 
taggtttta taaattaac tctaatttt atgcactac 
caaaaacgt ttccgattt gttcacgtc atgatattt 
ttattaatt tatttcgcg aaattatgt tgtgatcaa 
tagatttca agtatttgt acttaatag tttttacat 
agttatttg cagctgtca atataattg ttacaataa 
ggtctatta tagtttatt ccaactgtc atgttttta 
aagattttt attgaactg ttagatttt aaggaaacc 
atattgcct tttttagaa aagctagaa atgtaacag 
attggtttt gcttcatac taaaaatat ttaattagt 
tttaatacc ttgaatatg gcctatgat acggcaagc 
caatatgtt gatgagtga tgaatataa taatttgta 
tgtgggatt ggtaacgtt gtaaatata ttatttatt 
tatttcaat aatgtagat gcaatatta agaaatgat 
caaaaacag tggagtact tattgtctt tttcgtaac 
ttaagcaaa agacttgta tttcctgcc tgatacttt 
cttgttcat ccttctcat ctccaaatt gttgaacgc 
aacgtgcgt actacacaa ctccataac tgagtaatt 
ttatgatat ttttatagt aaaaaatta caataatta 
tgatttccc ccatgttat ttgagtatc aaaattttt 
gtaacccat aatctgtaa ctctcaatt ttaatagaa 
tatcaaaaa ctccaacag tgaagtcta atatgatcc 
tcaaacaaa aataaatat tcattgtta tg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY