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Gene information
General information
TC014317 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 1107147..1109081
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014317
    iBeetle screen iB_02274
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014317 RA
      MLAKPSLLV PLQSSRLLQ LLARLCHGK APDKPAPKK 
      SDLEVCTIA ECMVDPCKP EPAVVIVGA GIAGLSAAQ 
      RLAQCGLTN FTVLEATDR PGGRIHSCW LGDVIAEMG 
      AQFIEGGCI GNPVYNLAA QEGLLKPPL QRAKPLSGI 
      FCTSDGRAI DQPVAVLAY QTFKQIEHE AASLFSMGG 
      AKQHGSLLN FLSLRIQQE LQNFPDEQK YDVARIMYG 
      LTNAVRTKC GEDLSQISA DNYGSFIQI PGGQIRIPL 
      GFIGVLSPL MRELPENAL RLNKPVGNI RWGAVQARN 
      KGGPRAVVQ CCDGQEFPA DYVILTVSL GVLKEHADK 
      MFCPALPSS KMEAINNIG YGNVDKIFL DYDRPFWVW 
      CEGGINFAW SPDELANRT DWTKGLSAI EEVHGSKHV 
      LCAYISGPE AAIMEHASD EEVAEGITR ILRQFTGDA 
      SLPYPSTVL RSKWATDPF FCGSYSYMG LNSHVGHQC 
      DLSCPVPGT CEPIPPILL FAGEATCAG HHSTVHGAR 
      LSGIREAER VIQLTKSYG GPPPSI
    CDS sequence
    • >TC014317 RA
      atgttggca aaaccgtcc ctattagta cccctccag 
      tccagccgc ctcctgcaa ctactcgcc cgcctttgc 
      catggcaaa gcccccgac aagcccgcc cccaaaaaa 
      tccgacctc gaagtttgc acaatcgcc gagtgcatg 
      gtcgacccc tgcaagcct gaacctgcc gtcgtgatc 
      gtcggcgca ggaattgca ggattatca gcagcccaa 
      cgtttagcc caatgcggc ttgacgaac tttacagtc 
      ctggaagct accgacagg ccaggcggc aggattcat 
      tcctgctgg ctaggcgac gtcatcgcg gaaatggga 
      gcacagttc atagagggc ggctgtatc ggcaatccg 
      gtttataat ctagccgcc caggagggg ctcttaaag 
      ccgcccctg caaagggcc aaacccttg agtgggatt 
      ttttgtacc agcgatggg cgggctatt gaccagccg 
      gtggccgtt ttggcctat cagactttc aagcagata 
      gagcacgaa gcggccagt ctttttagt atgggcggc 
      gcgaaacaa catggttcg cttttgaat ttcttgagt 
      ttaaggatt cagcaggag ttgcagaac ttccccgat 
      gagcaaaag tacgacgtg gcgaggatt atgtacggg 
      ttgactaat gcagttagg acaaaatgt ggcgaggac 
      ttgagtcaa ataagtgcc gacaattac ggcagtttc 
      atccaaatt ccgggcggt caaatccgg attcctctc 
      ggcttcatc ggcgtcctc tcccccttg atgcgtgaa 
      ctccccgaa aacgccctc cgcctgaac aagcccgtg 
      ggtaacatt cgatggggc gccgtccag gcccgtaac 
      aaagggggc ccccgagcc gtagtccaa tgctgcgac 
      ggccaggag ttccccgcc gattacgtc atcctcacc 
      gtatcccta ggagtcctc aaggaacac gccgacaag 
      atgttctgt cccgctctc ccctccagc aaaatggaa 
      gccatcaac aacatcggc tacgggaac gtcgataaa 
      atcttcctc gactacgac cggcctttc tgggtctgg 
      tgcgagggc ggcattaat ttcgcctgg tctccagat 
      gaactcgcg aataggacc gactggacc aaaggcctc 
      agcgcgatc gaggaagtg cacgggagc aagcacgtc 
      ctctgtgcg tatatttcc ggcccagag gcggccatt 
      atggaacat gcgagtgac gaggaggtg gcggaaggg 
      attaccagg attttgagg cagttcaca ggggatgcc 
      tccctgccg tatccgtcg acggtgttg aggtccaaa 
      tgggcaacg gacccgttt ttctgcggg tcttacagt 
      tacatgggt ctgaattca cacgtgggg caccaatgc 
      gacttgagt tgcccggtt ccgggcacg tgtgagccc 
      atcccgccc attttgttg tttgcagga gaggccacg 
      tgtgctgga caccactcg acggtacat ggggcaagg 
      ctgagtgga atcagggag gcagaaagg gtgatccag 
      ttgacgaag tcatatgga gggccgcca ccgtcgatt 
      taa
    mRNA sequence
    • >TC014317 RA
      gtttgaaaa aaaatgttg gcaaaaccg tccctatta 
      gtacccctc cagtccagc cgcctcctg caactactc 
      gcccgcctt tgccatggc aaagccccc gacaagccc 
      gcccccaaa aaatccgac ctcgaagtt tgcacaatc 
      gccgagtgc atggtcgac ccctgcaag cctgaacct 
      gccgtcgtg atcgtcggc gcaggaatt gcaggatta 
      tcagcagcc caacgttta gcccaatgc ggcttgacg 
      aactttaca gtcctggaa gctaccgac aggccaggc 
      ggcaggatt cattcctgc tggctaggc gacgtcatc 
      gcggaaatg ggagcacag ttcatagag ggcggctgt 
      atcggcaat ccggtttat aatctagcc gcccaggag 
      gggctctta aagccgccc ctgcaaagg gccaaaccc 
      ttgagtggg attttttgt accagcgat gggcgggct 
      attgaccag ccggtggcc gttttggcc tatcagact 
      ttcaagcag atagagcac gaagcggcc agtcttttt 
      agtatgggc ggcgcgaaa caacatggt tcgcttttg 
      aatttcttg agtttaagg attcagcag gagttgcag 
      aacttcccc gatgagcaa aagtacgac gtggcgagg 
      attatgtac gggttgact aatgcagtt aggacaaaa 
      tgtggcgag gacttgagt caaataagt gccgacaat 
      tacggcagt ttcatccaa attccgggc ggtcaaatc 
      cggattcct ctcggcttc atcggcgtc ctctccccc 
      ttgatgcgt gaactcccc gaaaacgcc ctccgcctg 
      aacaagccc gtgggtaac attcgatgg ggcgccgtc 
      caggcccgt aacaaaggg ggcccccga gccgtagtc 
      caatgctgc gacggccag gagttcccc gccgattac 
      gtcatcctc accgtatcc ctaggagtc ctcaaggaa 
      cacgccgac aagatgttc tgtcccgct ctcccctcc 
      agcaaaatg gaagccatc aacaacatc ggctacggg 
      aacgtcgat aaaatcttc ctcgactac gaccggcct 
      ttctgggtc tggtgcgag ggcggcatt aatttcgcc 
      tggtctcca gatgaactc gcgaatagg accgactgg 
      accaaaggc ctcagcgcg atcgaggaa gtgcacggg 
      agcaagcac gtcctctgt gcgtatatt tccggccca 
      gaggcggcc attatggaa catgcgagt gacgaggag 
      gtggcggaa gggattacc aggattttg aggcagttc 
      acaggggat gcctccctg ccgtatccg tcgacggtg 
      ttgaggtcc aaatgggca acggacccg tttttctgc 
      gggtcttac agttacatg ggtctgaat tcacacgtg 
      gggcaccaa tgcgacttg agttgcccg gttccgggc 
      acgtgtgag cccatcccg cccattttg ttgtttgca 
      ggagaggcc acgtgtgct ggacaccac tcgacggta 
      catggggca aggctgagt ggaatcagg gaggcagaa 
      agggtgatc cagttgacg aagtcatat ggagggccg 
      ccaccgtcg atttaacag agggtcggt tgtttggtt 
      tttttgagt aaattggtc agtgtctag tcccgtatt 
      ttaataaaa g