Status
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Gene information
General information
TC014288 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8458915..8470586
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014288
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02271

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014288 RA
      MFASLKNKI REETGSDLS KLTAKITSS TVQRIDSLR 
      GRSHQGSSS SINSLVSSD GVKEDGPID SEELRKRIL 
      KIEAEFTRK LDQKELEWR EIVAEKDKQ LQSLEKEKE 
      EAQKQIQML RETLKSAEE FKQKLVEHQ EDKEQIENF 
      QTQELSKVK HLVLLREQE LAEKTAALK EATAQLEKL 
      RTEVSRLCR QEEQLSDLQ DDLESLRHS SSRDLAALA 
      TQLAKSEEE RRHLSDLVV VLRQRVANE TSDDEHVAS 
      ERRLLEQRL EEAHLHLAD IKTSWSDKI ASLETQVGR 
      LSRQAAEEG SERRRAVQE KETLAEKVR QMEAELECN 
      KLEINNKET KIKRLTLDV EELSKELNA LRSESEEEV 
      AFLRTQLQN ALTEVKVVR KNLENTESE LDRTGEECS 
      KLKISVDSE HEANSSLRL IITKLEKEL SEEKTNSLN 
      VQKTLSRVT AEKNTALLR NAEVSQQME IIKQEKRRQ 
      EGEMNDLLG KLSQLEEES RKHNESKAM ELELRNNIA 
      ELEDQISEK NKNIKTLQL RLADMKKTL QQELRTPGN 
      PNYHSDLVE NSAAILTPS QVSTKSFPS VVRRDEDDV 
      NFKYLKHVV IKFLTSREY EAQHLTKAI ATLLKFTAE 
      EEKLVNDTL EWKRSWFGS KPKLGNGQK ARALPHS
    CDS sequence
    • >TC014288 RA
      atgtttgcc tctctgaaa aacaaaatt cgggaggaa 
      actggcagc gacctgtcg aaactaacc gcgaaaata 
      acctcctcc acggtccag agaatagac tcgttaagg 
      gggcgctcg caccagggc tcctcctca agcatcaac 
      tccctcgta tcgtcggac ggtgttaag gaggatggc 
      cctatcgat tctgaagag ctacgcaaa cgaatattg 
      aaaatcgag gctgaattc acgcggaag ttggatcag 
      aaggaacta gaatggagg gaaatagtg gccgagaag 
      gacaagcag ttgcaaagt ctggagaag gagaaagaa 
      gaggcccaa aaacaaatc cagatgctc agagagacg 
      ctgaaaagc gccgaagag ttcaagcag aagttggtg 
      gagcaccag gaggacaaa gagcagatt gaaaacttc 
      caaactcag gaattaagc aaagtcaaa cacttggtg 
      ttactgagg gagcaagag ctggccgag aagacggca 
      gccttgaag gaggccacc gcccagttg gagaaactc 
      cgcactgag gtgtcccga ctctgccgc caggaggag 
      caattaagt gatttacag gacgacttg gagtcgttg 
      agacactcc agttcgcgc gatttggcc gctttggcc 
      acccagttg gccaaaagc gaggaggaa aggcgtcat 
      ttgtccgac cttgttgtt gttttgaga cagagagtg 
      gccaatgag acctcagac gacgaacat gtcgcctct 
      gagaggcgc ctgttggag cagaggctc gaagaggcg 
      catttgcac ctagctgac attaagacc tcctggagc 
      gataaaatc gcctcgcta gagacccag gtggggcgc 
      ttgagcaga caggcggct gaagaaggc agcgagaga 
      cggcgggcg gtgcaggag aaggaaacc ctggccgag 
      aaagtgcgg caaatggaa gcggaatta gagtgtaat 
      aaactagaa attaataat aaagagacg aaaatcaag 
      cgattgact ttggatgtt gaagagttg tccaaggag 
      ctgaacgct ctgcggtct gaatctgaa gaggaagtt 
      gcgttcctc aggacccaa cttcaaaat gcccttaca 
      gaggtgaag gtcgttcgc aagaatctg gagaacacc 
      gaaagtgag ttggacagg accggggaa gagtgtagc 
      aaattgaaa atctcggtg gattcggaa catgaggcg 
      aacagttcg ctcaggctg ataataacc aagttggag 
      aaggaactt agcgaagaa aagacgaat tcgttgaat 
      gtgcagaaa accttgtcg agggtcact gctgagaaa 
      aatacggca ctgttgcgt aatgctgaa gtttcgcaa 
      cagatggag ataatcaag caggagaaa aggcgacag 
      gagggagaa atgaacgac ttgcttggc aaactgagt 
      caattagaa gaggagagt aggaaacat aacgagagt 
      aaagcgatg gaactggag ttgaggaat aacatcgcg 
      gagttggaa gatcaaata tcggagaaa aataagaat 
      atcaagact ctccagctg cgcctggcc gacatgaag 
      aaaaccctg cagcaagag ttgcgaacg ccaggtaac 
      ccgaattat catagtgac ttagtggaa aattcagca 
      gctattctc actcccagt caagtatca acaaaaagt 
      tttcccagt gtggtgaga agggacgaa gacgatgtt 
      aatttcaaa tatttaaaa catgtagtg attaaattt 
      ttaacgagc cgggagtac gaagctcaa cacttaact 
      aaagcaata gccacctta cttaagttt acagccgag 
      gaggaaaaa ttagtcaat gatacttta gaatggaag 
      agatcgtgg ttcggctcg aaaccgaag ctggggaac 
      ggccagaaa gcccgggct ttgccgcac agctga
    mRNA sequence
    • >TC014288 RA
      cttccaagc tgatgtcga tgtggtgat gcagatgct 
      gctgtagcc attcatcat tttcttaat aatttctgt 
      gtgagctaa tcgataaat tttattatc ataattttg 
      caaacgacg ttcaatggc ttcctctcc tttcagcag 
      accttcaag ccaaatcag tccaaggac acgcacgcg 
      attggtgtg caaacgtca gcggttgtt gtcacacct 
      gcctgtcac ctgtcggtg aactactaa taaaattgt 
      taattatta tcacagtta ttatcaaca aatacgtgt 
      aaatgcgcg tacccgacc gttttgcca atagttgtg 
      ttcacattt tcgaaaatt ctgccacat tttgcaaaa 
      ccagtgctc aataatgcc cgtaaaata gctccagtg 
      cgtgaaaca tgtttgcct ctctgaaaa acaaaattc 
      gggaggaaa ctggcagcg acctgtcga aactaaccg 
      cgaaaataa cctcctcca cggtccaga gaatagact 
      cgttaaggg ggcgctcgc accagggct cctcctcaa 
      gcatcaact ccctcgtat cgtcggacg gtgttaagg 
      aggatggcc ctatcgatt ctgaagagc tacgcaaac 
      gaatattga aaatcgagg ctgaattca cgcggaagt 
      tggatcaga aggaactag aatggaggg aaatagtgg 
      ccgagaagg acaagcagt tgcaaagtc tggagaagg 
      agaaagaag aggcccaaa aacaaatcc agatgctca 
      gagagacgc tgaaaagcg ccgaagagt tcaagcaga 
      agttggtgg agcaccagg aggacaaag agcagattg 
      aaaacttcc aaactcagg aattaagca aagtcaaac 
      acttggtgt tactgaggg agcaagagc tggccgaga 
      agacggcag ccttgaagg aggccaccg cccagttgg 
      agaaactcc gcactgagg tgtcccgac tctgccgcc 
      aggaggagc aattaagtg atttacagg acgacttgg 
      agtcgttga gacactcca gttcgcgcg atttggccg 
      ctttggcca cccagttgg ccaaaagcg aggaggaaa 
      ggcgtcatt tgtccgacc ttgttgttg ttttgagac 
      agagagtgg ccaatgaga cctcagacg acgaacatg 
      tcgcctctg agaggcgcc tgttggagc agaggctcg 
      aagaggcgc atttgcacc tagctgaca ttaagacct 
      cctggagcg ataaaatcg cctcgctag agacccagg 
      tggggcgct tgagcagac aggcggctg aagaaggca 
      gcgagagac ggcgggcgg tgcaggaga aggaaaccc 
      tggccgaga aagtgcggc aaatggaag cggaattag 
      agtgtaata aactagaaa ttaataata aagagacga 
      aaatcaagc gattgactt tggatgttg aagagttgt 
      ccaaggagc tgaacgctc tgcggtctg aatctgaag 
      aggaagttg cgttcctca ggacccaac ttcaaaatg 
      cccttacag aggtgaagg tcgttcgca agaatctgg 
      agaacaccg aaagtgagt tggacagga ccggggaag 
      agtgtagca aattgaaaa tctcggtgg attcggaac 
      atgaggcga acagttcgc tcaggctga taataacca 
      agttggaga aggaactta gcgaagaaa agacgaatt 
      cgttgaatg tgcagaaaa ccttgtcga gggtcactg 
      ctgagaaaa atacggcac tgttgcgta atgctgaag 
      tttcgcaac agatggaga taatcaagc aggagaaaa 
      ggcgacagg agggagaaa tgaacgact tgcttggca 
      aactgagtc aattagaag aggagagta ggaaacata 
      acgagagta aagcgatgg aactggagt tgaggaata 
      acatcgcgg agttggaag atcaaatat cggagaaaa 
      ataagaata tcaagactc tccagctgc gcctggccg 
      acatgaaga aaaccctgc agcaagagt tgcgaacgc 
      caggtaacc cgaattatc atagtgact tagtggaaa 
      attcagcag ctattctca ctcccagtc aagtatcaa 
      caaaaagtt ttcccagtg tggtgagaa gggacgaag 
      acgatgtta atttcaaat atttaaaac atgtagtga 
      ttaaatttt taacgagcc gggagtacg aagctcaac 
      acttaacta aagcaatag ccaccttac ttaagttta 
      cagccgagg aggaaaaat tagtcaatg atactttag 
      aatggaaga gatcgtggt tcggctcga aaccgaagc 
      tggggaacg gccagaaag cccgggctt tgccgcaca 
      gctgatggt tgctttaat tcttgtgtt gtgatagct 
      cgaccattc cgcttgggg catgtgttt cttccattt 
      taatacttg cacgtttct gttccctgt tttattgta 
      gcgattaag acttattta ttctatttt tataaagca 
      aatgcccgg ataggatag ttgtacaaa attgtaaat 
      accaacaag tttatttgt caattttta aatcgtgga 
      tgctttata ctgtattta ttatttttg aaagtttaa 
      ataaaacta cagattgtt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY