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Gene information
General information
TC033115
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014279
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 448191..449433
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033115
    iBeetle screen iB_02270
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033115 RA
      MDRVVICAS EICATAILV FLGCAGCIN FGIPPNHLQ 
      ICLTFGLAV MVSVQCFGH ISGSHINPL VTVAAATLG 
      NIPLIQVPI YFVGQMLGA LSGFGLVKL ATPTEYFET 
      NSTVGLCSP ALHEGVTPF QGLLIEFLI SLMLTLVCC 
      GVWDCRNNT KHDSVPLRF GLAIAVLAL SGGPYTGGN 
      MNPARSFAP ALINGDWDN HWIYWVGPL SASFIGALF 
      YRFLFGKEP VEEEQGNVA ETIALNDKA
    CDS sequence
    • >TC033115 RA
      atggaccga gtggtaatt tgtgcctcg gagatttgt 
      gcaactgca atccttgta tttcttgga tgcgccggc 
      tgcataaac ttcggaatc cctcctaat cacttacag 
      atctgcctt actttcggt ctcgctgtc atggtctca 
      gtccagtgt ttcggccac atttccgga tctcacatt 
      aacccactg gtgacagta gcggccgcc actttgggt 
      aacattcca ctaattcaa gtacctatt tatttcgtg 
      ggccaaatg ttgggtgca ttgtctggt ttcggctta 
      gtgaagtta gcaaccccc actgaatat ttcgagacc 
      aactcgaca gtgggcttg tgttcaccg gccctccac 
      gagggagtc acccccttc cagggcctt ttgatcgag 
      tttctaatt tcgctaatg ctcacactt gtgtgttgt 
      ggcgtttgg gattgcagg aataacaca aagcacgac 
      tcagtcccg ctcaggttc gggctggct attgccgtt 
      ttggcctta tctgggggg ccgtacact ggagggaac 
      atgaatccg gctaggagt ttcgctccg gctttgatc 
      aatggagac tgggacaac cattggatc tactgggtt 
      ggaccgctg agtgcttcg tttatcggc gctctgttc 
      tataggttc ctgtttggg aaggaacca gtggaggag 
      gagcaaggc aatgtcgcc gaaactatt gctttgaat 
      gataaagct taa
    mRNA sequence
    • >TC033115 RA
      ttttccagg gaaaaaaat ccaagtcgg tgataactt 
      aaccacgat ggaccgagt ggtaatttg tgcctcgga 
      gatttgtgc aactgcaat ccttgtatt tcttggatg 
      cgccggctg cataaactt cggaatccc tcctaatca 
      cttacagat ctgccttac tttcggtct cgctgtcat 
      ggtctcagt ccagtgttt cggccacat ttccggatc 
      tcacattaa cccactggt gacagtagc ggccgccac 
      tttgggtaa cattccact aattcaagt acctattta 
      tttcgtggg ccaaatgtt gggtgcatt gtctggttt 
      cggcttagt gaagttagc aacccccac tgaatattt 
      cgagaccaa ctcgacagt gggcttgtg ttcaccggc 
      cctccacga gggagtcac ccccttcca gggcctttt 
      gatcgagtt tctaatttc gctaatgct cacacttgt 
      gtgttgtgg cgtttggga ttgcaggaa taacacaaa 
      gcacgactc agtcccgct caggttcgg gctggctat 
      tgccgtttt ggccttatc tggggggcc gtacactgg 
      agggaacat gaatccggc taggagttt cgctccggc 
      tttgatcaa tggagactg ggacaacca ttggatcta 
      ctgggttgg accgctgag tgcttcgtt tatcggcgc 
      tctgttcta taggttcct gtttgggaa ggaaccagt 
      ggaggagga gcaaggcaa tgtcgccga aactattgc 
      tttgaatga taaagctta actcagttt atgtaatta 
      attacattt tttaaatta ttaaatact gtgtgttta 
      aaatgtcgg cacaattac ggtttctca agtacatgt 
      tattgcgta tgctttcac gtaagctgc cttgaaaat 
      tttttacca attgtaagg attttttac aacaaattg 
      taagaaatg taaagtgcc ggaaaactc ctttacacc 
      tatttcatt gcgtcttca tcaaatttt acagtacaa 
      acgtatcct tgtagatca atttgagaa ataaacttg 
      tattttaat t