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Gene information
General information
TC014277 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8344215..8350970
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014277
      iBeetle screen iB_02269

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      Fertility
      previtellogenic egg chamber not present - Number of animals showing the phenotype: 3

      tropharium size decreased - Number of animals showing the phenotype: 3

      vitellogenic egg chamber fused - Number of animals showing the phenotype: 3

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014277 RA
        MSDMGDDDL LGGDSDCEP YDFSEEKEE LLLQGDDDY 
        SKTEAVYKK ASNSDSDDQ DDVLNLDIE EDFQDVDDK 
        LLFEEEQEP KVKKDEKFS SEKKPQNTQ HIPVVKTHP 
        IAPKKQDNA PPKERSAPK VLTRNDNRW RNPGRGNSQ 
        QRPNYAKNN FQRRRNGTV LINPRYEGT VRVSENTRL 
        AWDNNAQYP QNNTSNYVQ PWVSGNNLN YETTPQNQI 
        FNNLAALLP QTSNSIFQP PVNSYQPTL SFEPPPGIQ 
        TSPPPYNTQ VQPYCQWNS PSVDNQVSR GQYAAQSST 
        SSHQPCLPR GPQNFNNSY TNYYNSSFN LQNYSSYNV 
        RPNYETRRK PYNNMPSQN TNFQNKNSS YASNNSYNR 
        NTNLSSVPK RPNYSSPHI VPIENRNQF NETRANSYR 
        NDFKQGQNQ NVQSGQQFQ TRTENRPNN TTFKKNNDN 
        FDVQRNRRT NDNSGAKSD QPEKRPKFD ESSNKKSAD 
        STKESKNED KKVPPDENI DFEDEETRL YRLKIEEQK 
        RERERILAL KEERRKQMM LQRKQEEAK KLPPPPPPP 
        QPENSANFS KPANFQRPQ FERVADTAT VQNQYAKPK 
        NSAQYSNVD SDLSDEFPN LTFKITNKI PQSAPVQST 
        PKTEASSSG FMSNRVVMV KGKTGGLPV SSTGAPKPS 
        NEVNESSNV GQGLSAFFN NRKVLKKDS SLLDTRLVV 
        VNNLSANIN QNKLVALTR GIGEVQVR
      • >TC014277 RB
        MSDMGDDDL LGGDSDCEP YDFSEEKEE LLLQGDDDY 
        SKTEAVYKK ASNSDSDDQ DDVLNLDIE EDFQDVDDK 
        LLFEEEQEP KVKKDEKFS SEKKPQNTQ HIPVVKTHP 
        IAPKKQDNA PPKERSAPK VLTRNDNRW RNPGRGNSQ 
        QRPNYAKNN FQRRRNGTV LINPRYEGT VRVSENTRL 
        AWDNNAQYP QNNTSNYVQ PWVSGNNLN YETTPQNQI 
        FNNLAALLP QTSNSIFQP PVNSYQPTL SFEPPPGIQ 
        TSPPPYNTQ VQPYCQWNS PSVDNQVSR GQYAAQSST 
        SSHQPCLPR GPQNFNNSY TNYYNSSFN LQNYSSYNV 
        RPNYETRRK PYNNMPSQN TNFQNKNSS YASNNSYNR 
        NTNLSSVPK RPNYSSPHI VPIENRNQF NETRANSYR 
        NDFKQGQNQ NVQSGQQFQ TRTENRPNN TTFKKNNDN 
        FDVQRNRRT NDNSGAKSD QPEKRPKFD ESSNKKSAD 
        STKESKNED KKVPPDENI DFEDEETRL YRLKIEEQK 
        RERERILAL KEERRKQMM LQRKQEEAK KLPPPPPPP 
        QPENSANFS KPANFQRPQ FERVADTAT VQNQYAKPK 
        NSAQYSNVD SDLSDEFPN LTFKITNKI PQSAPVQST 
        PKTEASSSG FMSNRVVMV KGKTGGLPV SSTGAPKPS 
        NEVNESSNV GQGLSAFFN NRKVLKKDS SLLDTRLVV 
        VNNLSANIN QNKLVALTR GIGEVQKLR VDRQERQAT 
        ILFKSVASA HAFFKKYQR RILDDLSVI EVKLEPMI
      • >TC014277 RC
        MSDMGDDDL LGGDSDCEP YDFSEEKEE LLLQGDDDY 
        SKTEAVYKK ASNSDSDDQ DDVLNLDIE EDFQDVDDK 
        LLFEEEQEP KVKKDEKFS SEKKPQNTQ HIPVVKTHP 
        IAPKKQDNA PPKERSAPK VLTRNDNRW RNPGRGNSQ 
        QRPNYAKNN FQRRRNGTV LINPRYEGT VRVSENTRL 
        AWDNNAQYP QNNTSNYVQ PWVSGNNLN YETTPQNQI 
        FNNLAALLP QTSNSIFQP PVNSYQPTL SFEPPPGIQ 
        TSPPPYNTQ VQVSRGQYA AQSSTSSHQ PCLPRGPQN 
        FNNSYTNYY NSSFNLQNY SSYNVRPNY ETRRKPYNN 
        MPSQNTNFQ NKNSSYASN NSYNRNTNL SSVPKRPNY 
        SSPHIVPIE NRNQFNETR ANSYRNDFK QGQNQNVQS 
        GQQFQTRTE NRPNNTTFK KNNDNFDVQ RNRRTNDNS 
        GAKSDQPEK RPKFDESSN KKSADSTKE SKNEDKKVP 
        PDENIDFED EETRLYRLK IEEQKRERE RILALKEER 
        RKQMMLQRK QEEAKKLPP PPPPPQPEN SANFSKPAN 
        FQRPQFERV ADTATVQNQ YAKPKNSAQ YSNVDSDLS 
        DEFPNLTFK ITNKIPQSA PVQSTPKTE ASSSGFMSN 
        RVVMVKGKT GGLPVSSTG APKPSNEVN ESSNVGQGL 
        SAFFNNRKV LKKDSSLLD TRLVVVNNL SANINQNKL 
        VALTRGIGE VQVR
      • >TC014277 RD
        MSDMGDDDL LGGDSDCEP YDFSEEKEE LLLQGDDDY 
        SKTEAVYKK ASNSDSDDQ DDVLNLDIE EDFQDVDDK 
        LLFEEEQEP KVKKDEKFS SEKKPQNTQ HIPVVKTHP 
        IAPKKQDNV APPKERSAP KVLTRNDNR WRNPGRGNS 
        QQRPNYAKN NFQRRRNGT VLINPRYEG TVRVSENTR 
        LAWDNNAQY PQNNTSNYV QPWVSGNNL NYETTPQNQ 
        IFNNLAALL PQTSNSIFQ PPVNSYQPT LSFEPPPGI 
        QTSPPPYNT QVQPYCQWN SPSVDNQVS RGQYAAQSS 
        TSSHQPCLP RGPQNFNNS YTNYYNSSF NLQNYSSYN 
        VRPNYETRR KPYNNMPSQ NTNFQNKNS SYASNNSYN 
        RNTNLSSVP KRPNYSSPH IVPIENRNQ FNETRANSY 
        RNDFKQGQN QNVQSGQQF QTRTENRPN NTTFKKNND 
        NFDVQRNRR TNDNSGAKS DQPEKRPKF DESSNKKSA 
        DSTKESKNE DKKVPPDEN IDFEDEETR LYRLKIEEQ 
        KRERERILA LKEERRKQM MLQRKQEEA KKLPPPPPP 
        PQPENSANF SKPANFQRP QFERVADTA TVQNQYAKP 
        KNSAQYSNV DSDLSDEFP NLTFKITNK IPQSAPVQS 
        TPKTEASSS GFMSNRVVM VKGKTGGLP VSSTGAPKP 
        SNEVNESSN VGQGLSAFF NNRKVLKKD SSLLDTRLV 
        VVNNLSANI NQNKLVALT RGIGEVQVR
      CDS sequence
      • >TC014277 RA
        atgtccgat atgggtgac gacgatctt ttgggcggt 
        gattccgac tgtgaacct tacgacttc agtgaagag 
        aaagaggag cttctgctt caaggggat gacgactac 
        tcaaaaacg gaagcggtg tacaagaaa gcctcgaat 
        tccgattcc gatgatcaa gacgatgtt ctcaattta 
        gacattgag gaagatttt caagatgtt gatgataaa 
        cttttgttt gaggaggag caggaaccc aaagttaaa 
        aaagacgag aagttctca tcagaaaag aaaccacaa 
        aacacgcaa catataccg gtcgttaaa acccaccct 
        attgcaccc aagaaacaa gataatgcg cctcctaaa 
        gagagaagt gcgccaaag gttctgaca cgaaacgac 
        aacaggtgg aggaacccg ggacgcggc aattcccaa 
        caacgccca aactacgcc aaaaacaat tttcaaaga 
        cgcaggaac ggaaccgtt ctaatcaac ccaagatac 
        gagggaaca gtgcgtgtt agtgaaaat acccgactt 
        gcttgggat aacaacgct caatatccc caaaacaac 
        acatccaat tacgttcaa ccatgggtc tccggaaat 
        aatttaaac tacgaaacc actccccaa aatcaaatt 
        tttaataac ttagccgcg ctgttacct caaacttcg 
        aatagtatt ttccagcct ccagtgaac agttatcaa 
        ccaacgtta tcatttgaa cctccgcca ggcattcaa 
        acttctccg ccgccatac aatactcaa gtccagcct 
        tattgccag tggaattcg ccaagtgtt gataatcag 
        gtctctcga ggacagtat gcagcgcag tccagtaca 
        tcatctcat cagccgtgt ttaccaaga ggaccacaa 
        aatttcaac aactcatat acaaactac tataacagt 
        agtttcaat ttgcaaaat tattcttct tataacgtt 
        agaccaaac tatgaaact aggagaaaa ccttacaat 
        aatatgccc agccagaat accaacttt cagaacaaa 
        aattcaagt tacgccagt aacaattct tataataga 
        aatactaat cttagtagt gttccaaaa cgcccaaac 
        tatagtagt ccccacatt gttccaatt gaaaataga 
        aatcaattc aacgaaact cgagccaat tcttacaga 
        aatgatttt aaacaaggc caaaatcag aacgtacaa 
        agtggtcaa caatttcag accagaact gaaaataga 
        cctaacaat accacgttt aagaaaaat aatgataat 
        ttcgacgtg caacgaaat aggcgcaca aatgacaat 
        tctggcgct aaaagcgac caaccagaa aagcgacca 
        aaatttgac gaatctagt aataaaaag tcagcggac 
        tcaactaaa gaatcgaag aatgaggac aaaaaagta 
        cctccagac gaaaatata gatttcgaa gatgaagaa 
        acaagattg taccgtttg aagattgaa gaacagaag 
        cgcgaacgc gagcgaata ttggccctg aaagaagag 
        agaaggaag caaatgatg ctgcagaga aaacaagaa 
        gaagcgaaa aaattgcca ccgccgccg ccaccacca 
        caaccggaa aattccgcg aatttctcc aaacctgct 
        aactttcaa agaccgcag tttgaacgt gtcgctgac 
        acagccacc gttcaaaat caatacgcg aaaccgaaa 
        aattcggcc cagtattct aacgttgat tcggatctt 
        tctgacgaa tttcctaat cttacgttt aaaattacc 
        aataagatt ccgcaaagc gctccagtt caaagcacg 
        ccgaaaact gaagcgtcc tctagtggc tttatgtct 
        aatagagtg gtcatggtt aaggggaaa accggtggt 
        ttacccgtt tcgtcaact ggggctcct aaacccagc 
        aatgaggtt aacgaatct tccaatgta gggcagggt 
        ctgtcggct tttttcaat aatcggaag gtgttgaag 
        aaagatagt tcgttgctc gataccagg ctggttgtt 
        gtcaataat ttgtcggca aatattaat caaaacaaa 
        ttggtggct ttgactcga ggcattggc gaagtacag 
        gtaagataa
      • >TC014277 RB
        atgtccgat atgggtgac gacgatctt ttgggcggt 
        gattccgac tgtgaacct tacgacttc agtgaagag 
        aaagaggag cttctgctt caaggggat gacgactac 
        tcaaaaacg gaagcggtg tacaagaaa gcctcgaat 
        tccgattcc gatgatcaa gacgatgtt ctcaattta 
        gacattgag gaagatttt caagatgtt gatgataaa 
        cttttgttt gaggaggag caggaaccc aaagttaaa 
        aaagacgag aagttctca tcagaaaag aaaccacaa 
        aacacgcaa catataccg gtcgttaaa acccaccct 
        attgcaccc aagaaacaa gataatgcg cctcctaaa 
        gagagaagt gcgccaaag gttctgaca cgaaacgac 
        aacaggtgg aggaacccg ggacgcggc aattcccaa 
        caacgccca aactacgcc aaaaacaat tttcaaaga 
        cgcaggaac ggaaccgtt ctaatcaac ccaagatac 
        gagggaaca gtgcgtgtt agtgaaaat acccgactt 
        gcttgggat aacaacgct caatatccc caaaacaac 
        acatccaat tacgttcaa ccatgggtc tccggaaat 
        aatttaaac tacgaaacc actccccaa aatcaaatt 
        tttaataac ttagccgcg ctgttacct caaacttcg 
        aatagtatt ttccagcct ccagtgaac agttatcaa 
        ccaacgtta tcatttgaa cctccgcca ggcattcaa 
        acttctccg ccgccatac aatactcaa gtccagcct 
        tattgccag tggaattcg ccaagtgtt gataatcag 
        gtctctcga ggacagtat gcagcgcag tccagtaca 
        tcatctcat cagccgtgt ttaccaaga ggaccacaa 
        aatttcaac aactcatat acaaactac tataacagt 
        agtttcaat ttgcaaaat tattcttct tataacgtt 
        agaccaaac tatgaaact aggagaaaa ccttacaat 
        aatatgccc agccagaat accaacttt cagaacaaa 
        aattcaagt tacgccagt aacaattct tataataga 
        aatactaat cttagtagt gttccaaaa cgcccaaac 
        tatagtagt ccccacatt gttccaatt gaaaataga 
        aatcaattc aacgaaact cgagccaat tcttacaga 
        aatgatttt aaacaaggc caaaatcag aacgtacaa 
        agtggtcaa caatttcag accagaact gaaaataga 
        cctaacaat accacgttt aagaaaaat aatgataat 
        ttcgacgtg caacgaaat aggcgcaca aatgacaat 
        tctggcgct aaaagcgac caaccagaa aagcgacca 
        aaatttgac gaatctagt aataaaaag tcagcggac 
        tcaactaaa gaatcgaag aatgaggac aaaaaagta 
        cctccagac gaaaatata gatttcgaa gatgaagaa 
        acaagattg taccgtttg aagattgaa gaacagaag 
        cgcgaacgc gagcgaata ttggccctg aaagaagag 
        agaaggaag caaatgatg ctgcagaga aaacaagaa 
        gaagcgaaa aaattgcca ccgccgccg ccaccacca 
        caaccggaa aattccgcg aatttctcc aaacctgct 
        aactttcaa agaccgcag tttgaacgt gtcgctgac 
        acagccacc gttcaaaat caatacgcg aaaccgaaa 
        aattcggcc cagtattct aacgttgat tcggatctt 
        tctgacgaa tttcctaat cttacgttt aaaattacc 
        aataagatt ccgcaaagc gctccagtt caaagcacg 
        ccgaaaact gaagcgtcc tctagtggc tttatgtct 
        aatagagtg gtcatggtt aaggggaaa accggtggt 
        ttacccgtt tcgtcaact ggggctcct aaacccagc 
        aatgaggtt aacgaatct tccaatgta gggcagggt 
        ctgtcggct tttttcaat aatcggaag gtgttgaag 
        aaagatagt tcgttgctc gataccagg ctggttgtt 
        gtcaataat ttgtcggca aatattaat caaaacaaa 
        ttggtggct ttgactcga ggcattggc gaagtacag 
        aaactgcga gtggatagg caagagcga caagcaaca 
        attctattt aaatctgtt gcatccgct catgcattt 
        tttaagaaa tatcaacgg cgaatactt gatgatttg 
        tctgtgatt gaagttaag ttagaaccg atgatttaa
      • >TC014277 RC
        atgtccgat atgggtgac gacgatctt ttgggcggt 
        gattccgac tgtgaacct tacgacttc agtgaagag 
        aaagaggag cttctgctt caaggggat gacgactac 
        tcaaaaacg gaagcggtg tacaagaaa gcctcgaat 
        tccgattcc gatgatcaa gacgatgtt ctcaattta 
        gacattgag gaagatttt caagatgtt gatgataaa 
        cttttgttt gaggaggag caggaaccc aaagttaaa 
        aaagacgag aagttctca tcagaaaag aaaccacaa 
        aacacgcaa catataccg gtcgttaaa acccaccct 
        attgcaccc aagaaacaa gataatgcg cctcctaaa 
        gagagaagt gcgccaaag gttctgaca cgaaacgac 
        aacaggtgg aggaacccg ggacgcggc aattcccaa 
        caacgccca aactacgcc aaaaacaat tttcaaaga 
        cgcaggaac ggaaccgtt ctaatcaac ccaagatac 
        gagggaaca gtgcgtgtt agtgaaaat acccgactt 
        gcttgggat aacaacgct caatatccc caaaacaac 
        acatccaat tacgttcaa ccatgggtc tccggaaat 
        aatttaaac tacgaaacc actccccaa aatcaaatt 
        tttaataac ttagccgcg ctgttacct caaacttcg 
        aatagtatt ttccagcct ccagtgaac agttatcaa 
        ccaacgtta tcatttgaa cctccgcca ggcattcaa 
        acttctccg ccgccatac aatactcaa gtccaggtc 
        tctcgagga cagtatgca gcgcagtcc agtacatca 
        tctcatcag ccgtgttta ccaagagga ccacaaaat 
        ttcaacaac tcatataca aactactat aacagtagt 
        ttcaatttg caaaattat tcttcttat aacgttaga 
        ccaaactat gaaactagg agaaaacct tacaataat 
        atgcccagc cagaatacc aactttcag aacaaaaat 
        tcaagttac gccagtaac aattcttat aatagaaat 
        actaatctt agtagtgtt ccaaaacgc ccaaactat 
        agtagtccc cacattgtt ccaattgaa aatagaaat 
        caattcaac gaaactcga gccaattct tacagaaat 
        gattttaaa caaggccaa aatcagaac gtacaaagt 
        ggtcaacaa tttcagacc agaactgaa aatagacct 
        aacaatacc acgtttaag aaaaataat gataatttc 
        gacgtgcaa cgaaatagg cgcacaaat gacaattct 
        ggcgctaaa agcgaccaa ccagaaaag cgaccaaaa 
        tttgacgaa tctagtaat aaaaagtca gcggactca 
        actaaagaa tcgaagaat gaggacaaa aaagtacct 
        ccagacgaa aatatagat ttcgaagat gaagaaaca 
        agattgtac cgtttgaag attgaagaa cagaagcgc 
        gaacgcgag cgaatattg gccctgaaa gaagagaga 
        aggaagcaa atgatgctg cagagaaaa caagaagaa 
        gcgaaaaaa ttgccaccg ccgccgcca ccaccacaa 
        ccggaaaat tccgcgaat ttctccaaa cctgctaac 
        tttcaaaga ccgcagttt gaacgtgtc gctgacaca 
        gccaccgtt caaaatcaa tacgcgaaa ccgaaaaat 
        tcggcccag tattctaac gttgattcg gatctttct 
        gacgaattt cctaatctt acgtttaaa attaccaat 
        aagattccg caaagcgct ccagttcaa agcacgccg 
        aaaactgaa gcgtcctct agtggcttt atgtctaat 
        agagtggtc atggttaag gggaaaacc ggtggttta 
        cccgtttcg tcaactggg gctcctaaa cccagcaat 
        gaggttaac gaatcttcc aatgtaggg cagggtctg 
        tcggctttt ttcaataat cggaaggtg ttgaagaaa 
        gatagttcg ttgctcgat accaggctg gttgttgtc 
        aataatttg tcggcaaat attaatcaa aacaaattg 
        gtggctttg actcgaggc attggcgaa gtacaggta 
        agataa
      • >TC014277 RD
        atgtccgat atgggtgac gacgatctt ttgggcggt 
        gattccgac tgtgaacct tacgacttc agtgaagag 
        aaagaggag cttctgctt caaggggat gacgactac 
        tcaaaaacg gaagcggtg tacaagaaa gcctcgaat 
        tccgattcc gatgatcaa gacgatgtt ctcaattta 
        gacattgag gaagatttt caagatgtt gatgataaa 
        cttttgttt gaggaggag caggaaccc aaagttaaa 
        aaagacgag aagttctca tcagaaaag aaaccacaa 
        aacacgcaa catataccg gtcgttaaa acccaccct 
        attgcaccc aagaaacaa gataatgta gcgcctcct 
        aaagagaga agtgcgcca aaggttctg acacgaaac 
        gacaacagg tggaggaac ccgggacgc ggcaattcc 
        caacaacgc ccaaactac gccaaaaac aattttcaa 
        agacgcagg aacggaacc gttctaatc aacccaaga 
        tacgaggga acagtgcgt gttagtgaa aatacccga 
        cttgcttgg gataacaac gctcaatat ccccaaaac 
        aacacatcc aattacgtt caaccatgg gtctccgga 
        aataattta aactacgaa accactccc caaaatcaa 
        atttttaat aacttagcc gcgctgtta cctcaaact 
        tcgaatagt attttccag cctccagtg aacagttat 
        caaccaacg ttatcattt gaacctccg ccaggcatt 
        caaacttct ccgccgcca tacaatact caagtccag 
        ccttattgc cagtggaat tcgccaagt gttgataat 
        caggtctct cgaggacag tatgcagcg cagtccagt 
        acatcatct catcagccg tgtttacca agaggacca 
        caaaatttc aacaactca tatacaaac tactataac 
        agtagtttc aatttgcaa aattattct tcttataac 
        gttagacca aactatgaa actaggaga aaaccttac 
        aataatatg cccagccag aataccaac tttcagaac 
        aaaaattca agttacgcc agtaacaat tcttataat 
        agaaatact aatcttagt agtgttcca aaacgccca 
        aactatagt agtccccac attgttcca attgaaaat 
        agaaatcaa ttcaacgaa actcgagcc aattcttac 
        agaaatgat tttaaacaa ggccaaaat cagaacgta 
        caaagtggt caacaattt cagaccaga actgaaaat 
        agacctaac aataccacg tttaagaaa aataatgat 
        aatttcgac gtgcaacga aataggcgc acaaatgac 
        aattctggc gctaaaagc gaccaacca gaaaagcga 
        ccaaaattt gacgaatct agtaataaa aagtcagcg 
        gactcaact aaagaatcg aagaatgag gacaaaaaa 
        gtacctcca gacgaaaat atagatttc gaagatgaa 
        gaaacaaga ttgtaccgt ttgaagatt gaagaacag 
        aagcgcgaa cgcgagcga atattggcc ctgaaagaa 
        gagagaagg aagcaaatg atgctgcag agaaaacaa 
        gaagaagcg aaaaaattg ccaccgccg ccgccacca 
        ccacaaccg gaaaattcc gcgaatttc tccaaacct 
        gctaacttt caaagaccg cagtttgaa cgtgtcgct 
        gacacagcc accgttcaa aatcaatac gcgaaaccg 
        aaaaattcg gcccagtat tctaacgtt gattcggat 
        ctttctgac gaatttcct aatcttacg tttaaaatt 
        accaataag attccgcaa agcgctcca gttcaaagc 
        acgccgaaa actgaagcg tcctctagt ggctttatg 
        tctaataga gtggtcatg gttaagggg aaaaccggt 
        ggtttaccc gtttcgtca actggggct cctaaaccc 
        agcaatgag gttaacgaa tcttccaat gtagggcag 
        ggtctgtcg gcttttttc aataatcgg aaggtgttg 
        aagaaagat agttcgttg ctcgatacc aggctggtt 
        gttgtcaat aatttgtcg gcaaatatt aatcaaaac 
        aaattggtg gctttgact cgaggcatt ggcgaagta 
        caggtaaga taa
      mRNA sequence
      • >TC014277 RA
        ggcttcggc aaagttcgt taactttcg tcagcaatt 
        tgttaacct acaaacaac cacaaaact catgttttt 
        gggcctttt ttcttgaaa aatttgcgt tcaaagttt 
        aaattagca gtctgatcg gtaatgtaa gttagtatt 
        tttcaaatt tacttcgaa tagtgtttt ttacaacct 
        cacaaaatg tccgatatg ggtgacgac gatcttttg 
        ggcggtgat tccgactgt gaaccttac gacttcagt 
        gaagagaaa gaggagctt ctgcttcaa ggggatgac 
        gactactca aaaacggaa gcggtgtac aagaaagcc 
        tcgaattcc gattccgat gatcaagac gatgttctc 
        aatttagac attgaggaa gattttcaa gatgttgat 
        gataaactt ttgtttgag gaggagcag gaacccaaa 
        gttaaaaaa gacgagaag ttctcatca gaaaagaaa 
        ccacaaaac acgcaacat ataccggtc gttaaaacc 
        caccctatt gcacccaag aaacaagat aatgcgcct 
        cctaaagag agaagtgcg ccaaaggtt ctgacacga 
        aacgacaac aggtggagg aacccggga cgcggcaat 
        tcccaacaa cgcccaaac tacgccaaa aacaatttt 
        caaagacgc aggaacgga accgttcta atcaaccca 
        agatacgag ggaacagtg cgtgttagt gaaaatacc 
        cgacttgct tgggataac aacgctcaa tatccccaa 
        aacaacaca tccaattac gttcaacca tgggtctcc 
        ggaaataat ttaaactac gaaaccact ccccaaaat 
        caaattttt aataactta gccgcgctg ttacctcaa 
        acttcgaat agtattttc cagcctcca gtgaacagt 
        tatcaacca acgttatca tttgaacct ccgccaggc 
        attcaaact tctccgccg ccatacaat actcaagtc 
        cagccttat tgccagtgg aattcgcca agtgttgat 
        aatcaggtc tctcgagga cagtatgca gcgcagtcc 
        agtacatca tctcatcag ccgtgttta ccaagagga 
        ccacaaaat ttcaacaac tcatataca aactactat 
        aacagtagt ttcaatttg caaaattat tcttcttat 
        aacgttaga ccaaactat gaaactagg agaaaacct 
        tacaataat atgcccagc cagaatacc aactttcag 
        aacaaaaat tcaagttac gccagtaac aattcttat 
        aatagaaat actaatctt agtagtgtt ccaaaacgc 
        ccaaactat agtagtccc cacattgtt ccaattgaa 
        aatagaaat caattcaac gaaactcga gccaattct 
        tacagaaat gattttaaa caaggccaa aatcagaac 
        gtacaaagt ggtcaacaa tttcagacc agaactgaa 
        aatagacct aacaatacc acgtttaag aaaaataat 
        gataatttc gacgtgcaa cgaaatagg cgcacaaat 
        gacaattct ggcgctaaa agcgaccaa ccagaaaag 
        cgaccaaaa tttgacgaa tctagtaat aaaaagtca 
        gcggactca actaaagaa tcgaagaat gaggacaaa 
        aaagtacct ccagacgaa aatatagat ttcgaagat 
        gaagaaaca agattgtac cgtttgaag attgaagaa 
        cagaagcgc gaacgcgag cgaatattg gccctgaaa 
        gaagagaga aggaagcaa atgatgctg cagagaaaa 
        caagaagaa gcgaaaaaa ttgccaccg ccgccgcca 
        ccaccacaa ccggaaaat tccgcgaat ttctccaaa 
        cctgctaac tttcaaaga ccgcagttt gaacgtgtc 
        gctgacaca gccaccgtt caaaatcaa tacgcgaaa 
        ccgaaaaat tcggcccag tattctaac gttgattcg 
        gatctttct gacgaattt cctaatctt acgtttaaa 
        attaccaat aagattccg caaagcgct ccagttcaa 
        agcacgccg aaaactgaa gcgtcctct agtggcttt 
        atgtctaat agagtggtc atggttaag gggaaaacc 
        ggtggttta cccgtttcg tcaactggg gctcctaaa 
        cccagcaat gaggttaac gaatcttcc aatgtaggg 
        cagggtctg tcggctttt ttcaataat cggaaggtg 
        ttgaagaaa gatagttcg ttgctcgat accaggctg 
        gttgttgtc aataatttg tcggcaaat attaatcaa 
        aacaaattg gtggctttg actcgaggc attggcgaa 
        gtacaggta agataactg attttttta ttctcttgg 
        aggggtttc cggttccca ttttcacca actcgattt 
        ttcgttttg tcgatattt tccacttaa atttgtttc 
        ttggttgta acttttcca cgatttaaa atttttaat 
        tttggataa gaggctttt attgaacgt cggctttag 
        cttttctga gtttcgatt tttttcgta aaaatcttt 
        aggtaagat gtggtcgtg aggtcatgt tcatcgaaa 
        acgagtttt ccactgaag gttttttct ctcaatgac 
        ttattttct tttaattgt tattcatgg tttaacaaa 
        gaaataaat tttaattcc acttccttc tagtacctg 
        aggtgccat ggattttat gtgattttg aaagctgtg 
        attacaaag ttttattac gaattcagt gaaatttaa 
        tattaaatt ttaattgac ttttggacc ttttcaata 
        aaaataatt cattttc
      • >TC014277 RB
        ggcttcggc aaagttcgt taactttcg tcagcaatt 
        tgttaacct acaaacaac cacaaaact catgttttt 
        gggcctttt ttcttgaaa aatttgcgt tcaaagttt 
        aaattagca gtctgatcg gtaatgtaa gttagtatt 
        tttcaaatt tacttcgaa tagtgtttt ttacaacct 
        cacaaaatg tccgatatg ggtgacgac gatcttttg 
        ggcggtgat tccgactgt gaaccttac gacttcagt 
        gaagagaaa gaggagctt ctgcttcaa ggggatgac 
        gactactca aaaacggaa gcggtgtac aagaaagcc 
        tcgaattcc gattccgat gatcaagac gatgttctc 
        aatttagac attgaggaa gattttcaa gatgttgat 
        gataaactt ttgtttgag gaggagcag gaacccaaa 
        gttaaaaaa gacgagaag ttctcatca gaaaagaaa 
        ccacaaaac acgcaacat ataccggtc gttaaaacc 
        caccctatt gcacccaag aaacaagat aatgcgcct 
        cctaaagag agaagtgcg ccaaaggtt ctgacacga 
        aacgacaac aggtggagg aacccggga cgcggcaat 
        tcccaacaa cgcccaaac tacgccaaa aacaatttt 
        caaagacgc aggaacgga accgttcta atcaaccca 
        agatacgag ggaacagtg cgtgttagt gaaaatacc 
        cgacttgct tgggataac aacgctcaa tatccccaa 
        aacaacaca tccaattac gttcaacca tgggtctcc 
        ggaaataat ttaaactac gaaaccact ccccaaaat 
        caaattttt aataactta gccgcgctg ttacctcaa 
        acttcgaat agtattttc cagcctcca gtgaacagt 
        tatcaacca acgttatca tttgaacct ccgccaggc 
        attcaaact tctccgccg ccatacaat actcaagtc 
        cagccttat tgccagtgg aattcgcca agtgttgat 
        aatcaggtc tctcgagga cagtatgca gcgcagtcc 
        agtacatca tctcatcag ccgtgttta ccaagagga 
        ccacaaaat ttcaacaac tcatataca aactactat 
        aacagtagt ttcaatttg caaaattat tcttcttat 
        aacgttaga ccaaactat gaaactagg agaaaacct 
        tacaataat atgcccagc cagaatacc aactttcag 
        aacaaaaat tcaagttac gccagtaac aattcttat 
        aatagaaat actaatctt agtagtgtt ccaaaacgc 
        ccaaactat agtagtccc cacattgtt ccaattgaa 
        aatagaaat caattcaac gaaactcga gccaattct 
        tacagaaat gattttaaa caaggccaa aatcagaac 
        gtacaaagt ggtcaacaa tttcagacc agaactgaa 
        aatagacct aacaatacc acgtttaag aaaaataat 
        gataatttc gacgtgcaa cgaaatagg cgcacaaat 
        gacaattct ggcgctaaa agcgaccaa ccagaaaag 
        cgaccaaaa tttgacgaa tctagtaat aaaaagtca 
        gcggactca actaaagaa tcgaagaat gaggacaaa 
        aaagtacct ccagacgaa aatatagat ttcgaagat 
        gaagaaaca agattgtac cgtttgaag attgaagaa 
        cagaagcgc gaacgcgag cgaatattg gccctgaaa 
        gaagagaga aggaagcaa atgatgctg cagagaaaa 
        caagaagaa gcgaaaaaa ttgccaccg ccgccgcca 
        ccaccacaa ccggaaaat tccgcgaat ttctccaaa 
        cctgctaac tttcaaaga ccgcagttt gaacgtgtc 
        gctgacaca gccaccgtt caaaatcaa tacgcgaaa 
        ccgaaaaat tcggcccag tattctaac gttgattcg 
        gatctttct gacgaattt cctaatctt acgtttaaa 
        attaccaat aagattccg caaagcgct ccagttcaa 
        agcacgccg aaaactgaa gcgtcctct agtggcttt 
        atgtctaat agagtggtc atggttaag gggaaaacc 
        ggtggttta cccgtttcg tcaactggg gctcctaaa 
        cccagcaat gaggttaac gaatcttcc aatgtaggg 
        cagggtctg tcggctttt ttcaataat cggaaggtg 
        ttgaagaaa gatagttcg ttgctcgat accaggctg 
        gttgttgtc aataatttg tcggcaaat attaatcaa 
        aacaaattg gtggctttg actcgaggc attggcgaa 
        gtacagaaa ctgcgagtg gataggcaa gagcgacaa 
        gcaacaatt ctatttaaa tctgttgca tccgctcat 
        gcatttttt aagaaatat caacggcga atacttgat 
        gatttgtct gtgattgaa gttaagtta gaaccgatg 
        atttaaaaa ggcttagaa tagttgttt ttgattttt 
        tatttagtg tatggactt atgattttg ttttcacta 
        gtttttatt cgtttgtaa ttcttattc tgatattgt 
        aaatataaa tgatatatt gtgaattct ctgagtacc 
        aatatttta tcatgtagg ggatttcat aaataggag 
        tgtgcagcg tgattgtga atcattggt cgtcaaaat 
        tttacaaac tctgaactc tggcttttg gacatgttt 
        ccgcacaaa caatcaaat tttggttca taagtgttc 
        agaatgaag tcttcttcg ttggatctg agtttttca 
        tattgtgtt ttaaccgga cgaacaatt agtaataac 
        atgtccacg attatttcg atttgacgt cataattat 
        ttatgattt atttttaat gtatgttct tttaaatca 
        tttcgtttc gcgtgtggc caacaattg tttttatct 
        gagtttttt gtaaacatt aatatttag ttttggtgt 
        tgttcttat agacagacg accattgag ttaatttgt 
        ttcgtactt ttgtcagtt tcgtctatt ataataaag 
        agctttcaa tgtttataa ttacctgat tattcacca 
        catccacaa acatgcgaa acccgtact aaaaactga 
        aatttttat caaatttaa tttttgtaa caaacatcg 
        tcaataaat gtgaattat tttcccgac gaagtagtt 
        ttattggta gatattacc actgattaa tttaacaca 
        tcaccaaca ggatgattt atttcgcaa atttcacgc 
        ttaatggga acgtaatta tgggaatga caccataaa 
        cacagcagc cgtgtgaga tttttgtgt atctcgagg 
        cggaagtga caatacgaa ggtttcctg cttttataa 
        aagcaatat tatttcatc taaatcctt cactttcaa 
        atctgcacc gctttttgt ggccgcgtg tttatcagt 
        cttgtggtt gggcacaaa aaatcttca ttttcaagt 
        gttgaacaa tcgcctcaa atctaattt tatttatga 
        aattatttt caatctctg cgcgcgacc aagttgaat 
        gaaatcgta gaggggaac gtattcata aaatgtaat 
        gtgtgtttt atacctcta gtgactgtg tgccaacag 
        tgctttaca aaccaaata tttagtatt gacttacat 
        gtgacacaa aaatactac ttggcaagg aaaattcaa 
        ggtaagtta attttttta tttgagttt tttcacttg 
        gatggagat ctgcaacca caaaggatg aattctaaa 
        tttaaacaa taacacggc gtataaata gagcgtgga 
        ggtttgttt tttgttaag gaaaaagat ttttttcaa 
        agaatttta ttaaaactg gactagaaa a
      • >TC014277 RC
        ggcttcggc aaagttcgt taactttcg tcagcaatt 
        tgttaacct acaaacaac cacaaaact catgttttt 
        gggcctttt ttcttgaaa aatttgcgt tcaaagttt 
        aaattagca gtctgatcg gtaatgtaa gttagtatt 
        tttcaaatt tacttcgaa tagtgtttt ttacaacct 
        cacaaaatg tccgatatg ggtgacgac gatcttttg 
        ggcggtgat tccgactgt gaaccttac gacttcagt 
        gaagagaaa gaggagctt ctgcttcaa ggggatgac 
        gactactca aaaacggaa gcggtgtac aagaaagcc 
        tcgaattcc gattccgat gatcaagac gatgttctc 
        aatttagac attgaggaa gattttcaa gatgttgat 
        gataaactt ttgtttgag gaggagcag gaacccaaa 
        gttaaaaaa gacgagaag ttctcatca gaaaagaaa 
        ccacaaaac acgcaacat ataccggtc gttaaaacc 
        caccctatt gcacccaag aaacaagat aatgcgcct 
        cctaaagag agaagtgcg ccaaaggtt ctgacacga 
        aacgacaac aggtggagg aacccggga cgcggcaat 
        tcccaacaa cgcccaaac tacgccaaa aacaatttt 
        caaagacgc aggaacgga accgttcta atcaaccca 
        agatacgag ggaacagtg cgtgttagt gaaaatacc 
        cgacttgct tgggataac aacgctcaa tatccccaa 
        aacaacaca tccaattac gttcaacca tgggtctcc 
        ggaaataat ttaaactac gaaaccact ccccaaaat 
        caaattttt aataactta gccgcgctg ttacctcaa 
        acttcgaat agtattttc cagcctcca gtgaacagt 
        tatcaacca acgttatca tttgaacct ccgccaggc 
        attcaaact tctccgccg ccatacaat actcaagtc 
        caggtctct cgaggacag tatgcagcg cagtccagt 
        acatcatct catcagccg tgtttacca agaggacca 
        caaaatttc aacaactca tatacaaac tactataac 
        agtagtttc aatttgcaa aattattct tcttataac 
        gttagacca aactatgaa actaggaga aaaccttac 
        aataatatg cccagccag aataccaac tttcagaac 
        aaaaattca agttacgcc agtaacaat tcttataat 
        agaaatact aatcttagt agtgttcca aaacgccca 
        aactatagt agtccccac attgttcca attgaaaat 
        agaaatcaa ttcaacgaa actcgagcc aattcttac 
        agaaatgat tttaaacaa ggccaaaat cagaacgta 
        caaagtggt caacaattt cagaccaga actgaaaat 
        agacctaac aataccacg tttaagaaa aataatgat 
        aatttcgac gtgcaacga aataggcgc acaaatgac 
        aattctggc gctaaaagc gaccaacca gaaaagcga 
        ccaaaattt gacgaatct agtaataaa aagtcagcg 
        gactcaact aaagaatcg aagaatgag gacaaaaaa 
        gtacctcca gacgaaaat atagatttc gaagatgaa 
        gaaacaaga ttgtaccgt ttgaagatt gaagaacag 
        aagcgcgaa cgcgagcga atattggcc ctgaaagaa 
        gagagaagg aagcaaatg atgctgcag agaaaacaa 
        gaagaagcg aaaaaattg ccaccgccg ccgccacca 
        ccacaaccg gaaaattcc gcgaatttc tccaaacct 
        gctaacttt caaagaccg cagtttgaa cgtgtcgct 
        gacacagcc accgttcaa aatcaatac gcgaaaccg 
        aaaaattcg gcccagtat tctaacgtt gattcggat 
        ctttctgac gaatttcct aatcttacg tttaaaatt 
        accaataag attccgcaa agcgctcca gttcaaagc 
        acgccgaaa actgaagcg tcctctagt ggctttatg 
        tctaataga gtggtcatg gttaagggg aaaaccggt 
        ggtttaccc gtttcgtca actggggct cctaaaccc 
        agcaatgag gttaacgaa tcttccaat gtagggcag 
        ggtctgtcg gcttttttc aataatcgg aaggtgttg 
        aagaaagat agttcgttg ctcgatacc aggctggtt 
        gttgtcaat aatttgtcg gcaaatatt aatcaaaac 
        aaattggtg gctttgact cgaggcatt ggcgaagta 
        caggtaaga taactgatt tttttattc tcttggagg 
        ggtttccgg ttcccattt tcaccaact cgatttttc 
        gttttgtcg atattttcc acttaaatt tgtttcttg 
        gttgtaact tttccacga tttaaaatt tttaatttt 
        ggataagag gcttttatt gaacgtcgg ctttagctt 
        ttctgagtt tcgattttt ttcgtaaaa atctttagg 
        taagatgtg gtcgtgagg tcatgttca tcgaaaacg 
        agttttcca ctgaaggtt ttttctctc aatgactta 
        ttttctttt aattgttat tcatggttt aacaaagaa 
        ataaatttt aattccact tccttctag tacctgagg 
        tgccatgga ttttatgtg attttgaaa gctgtgatt 
        acaaagttt tattacgaa ttcagtgaa atttaatat 
        taaatttta attgacttt tggaccttt tcaataaaa 
        ataattcat tttc
      • >TC014277 RD
        ggcttcggc aaagttcgt taactttcg tcagcaatt 
        tgttaacct acaaacaac cacaaaact catgttttt 
        gggcctttt ttcttgaaa aatttgcgt tcaaagttt 
        aaattagca gtctgatcg gtaatgtaa gttagtatt 
        tttcaaatt tacttcgaa tagtgtttt ttacaacct 
        cacaaaatg tccgatatg ggtgacgac gatcttttg 
        ggcggtgat tccgactgt gaaccttac gacttcagt 
        gaagagaaa gaggagctt ctgcttcaa ggggatgac 
        gactactca aaaacggaa gcggtgtac aagaaagcc 
        tcgaattcc gattccgat gatcaagac gatgttctc 
        aatttagac attgaggaa gattttcaa gatgttgat 
        gataaactt ttgtttgag gaggagcag gaacccaaa 
        gttaaaaaa gacgagaag ttctcatca gaaaagaaa 
        ccacaaaac acgcaacat ataccggtc gttaaaacc 
        caccctatt gcacccaag aaacaagat aatgtagcg 
        cctcctaaa gagagaagt gcgccaaag gttctgaca 
        cgaaacgac aacaggtgg aggaacccg ggacgcggc 
        aattcccaa caacgccca aactacgcc aaaaacaat 
        tttcaaaga cgcaggaac ggaaccgtt ctaatcaac 
        ccaagatac gagggaaca gtgcgtgtt agtgaaaat 
        acccgactt gcttgggat aacaacgct caatatccc 
        caaaacaac acatccaat tacgttcaa ccatgggtc 
        tccggaaat aatttaaac tacgaaacc actccccaa 
        aatcaaatt tttaataac ttagccgcg ctgttacct 
        caaacttcg aatagtatt ttccagcct ccagtgaac 
        agttatcaa ccaacgtta tcatttgaa cctccgcca 
        ggcattcaa acttctccg ccgccatac aatactcaa 
        gtccagcct tattgccag tggaattcg ccaagtgtt 
        gataatcag gtctctcga ggacagtat gcagcgcag 
        tccagtaca tcatctcat cagccgtgt ttaccaaga 
        ggaccacaa aatttcaac aactcatat acaaactac 
        tataacagt agtttcaat ttgcaaaat tattcttct 
        tataacgtt agaccaaac tatgaaact aggagaaaa 
        ccttacaat aatatgccc agccagaat accaacttt 
        cagaacaaa aattcaagt tacgccagt aacaattct 
        tataataga aatactaat cttagtagt gttccaaaa 
        cgcccaaac tatagtagt ccccacatt gttccaatt 
        gaaaataga aatcaattc aacgaaact cgagccaat 
        tcttacaga aatgatttt aaacaaggc caaaatcag 
        aacgtacaa agtggtcaa caatttcag accagaact 
        gaaaataga cctaacaat accacgttt aagaaaaat 
        aatgataat ttcgacgtg caacgaaat aggcgcaca 
        aatgacaat tctggcgct aaaagcgac caaccagaa 
        aagcgacca aaatttgac gaatctagt aataaaaag 
        tcagcggac tcaactaaa gaatcgaag aatgaggac 
        aaaaaagta cctccagac gaaaatata gatttcgaa 
        gatgaagaa acaagattg taccgtttg aagattgaa 
        gaacagaag cgcgaacgc gagcgaata ttggccctg 
        aaagaagag agaaggaag caaatgatg ctgcagaga 
        aaacaagaa gaagcgaaa aaattgcca ccgccgccg 
        ccaccacca caaccggaa aattccgcg aatttctcc 
        aaacctgct aactttcaa agaccgcag tttgaacgt 
        gtcgctgac acagccacc gttcaaaat caatacgcg 
        aaaccgaaa aattcggcc cagtattct aacgttgat 
        tcggatctt tctgacgaa tttcctaat cttacgttt 
        aaaattacc aataagatt ccgcaaagc gctccagtt 
        caaagcacg ccgaaaact gaagcgtcc tctagtggc 
        tttatgtct aatagagtg gtcatggtt aaggggaaa 
        accggtggt ttacccgtt tcgtcaact ggggctcct 
        aaacccagc aatgaggtt aacgaatct tccaatgta 
        gggcagggt ctgtcggct tttttcaat aatcggaag 
        gtgttgaag aaagatagt tcgttgctc gataccagg 
        ctggttgtt gtcaataat ttgtcggca aatattaat 
        caaaacaaa ttggtggct ttgactcga ggcattggc 
        gaagtacag gtaagataa ctgattttt ttattctct 
        tggaggggt ttccggttc ccattttca ccaactcga 
        tttttcgtt ttgtcgata ttttccact taaatttgt 
        ttcttggtt gtaactttt ccacgattt aaaattttt 
        aattttgga taagaggct tttattgaa cgtcggctt 
        tagcttttc tgagtttcg atttttttc gtaaaaatc 
        tttaggtaa gatgtggtc gtgaggtca tgttcatcg 
        aaaacgagt tttccactg aaggttttt tctctcaat 
        gacttattt tcttttaat tgttattca tggtttaac 
        aaagaaata aattttaat tccacttcc ttctagtac 
        ctgaggtgc catggattt tatgtgatt ttgaaagct 
        gtgattaca aagttttat tacgaattc agtgaaatt 
        taatattaa attttaatt gacttttgg accttttca 
        ataaaaata attcatttt c
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